CELLULAR BIOLOGY
SPRING 2011
CELL BIOLOGY
General Information – Spring 2011
1.
Instructor:
Office:
Office Hours:
Email:
Dr. John Sternfeld
225 Bowers Hall.
MWF 3-4 pm; Th 10-12 am
sternfeld@cortland.edu
Dr. Lisa DiMarco
313 Bowers
M 8:00-10:00, Tuesday 1:30-3:00
dimarcol@cortland.edu
2.
Course Materials:
Text:
Lab Manual:
Cell and Molecular Biology (6th edition) by Gerald Karp.
Laboratory Investigations in Cell and Molecular Biology by Allyn Bregman.
3. Communication: We want to keep open lines of communication. One way we will do this is through email, but
also material will be posted to a web site: “web.cortland.edu/sternfeld”. (Note that this is “web”, not “www”.) We
will be using this site to make announcements and to post lab data.
4. Labs: There will be lab every full week during the semester (see attached Laboratory Schedule). There are three
elements to the lab grade. (1) There will be 10 quizzes each worth 10 points. The lowest quiz grade will be dropped.
No make-ups will be given for missed quizzes. (2) There will be 2 Lab Reports, each worth 25 points. (3) There will
be a laboratory computer exercise you may do on your own time. This exercise will be graded by a 20-point practical
examination on March 9th. The lab, as a whole, will be worth a total of 160 points.
5. Lecture Exams: The lecture part of the course will be worth 300 points. There will be two hour exams, each
worth 70 points and a comprehensive final worth 120 points. The exact timing of the hour exams will be a little
flexible, but must occur approximately one third and two thirds through the semester. There will be quizzes and
homework assignments worth a total of 40 points.
Make-up exams. Only under unusual circumstances will I give a make-up for one of the hour exams. All make-ups
will be held on May 3rd. There will be no make-ups for quizzes or homework.
6. Grading: For each hour exam I will make a graph (histogram) plotting everyone’s average. I will post the
histograms so that you will be able to determine exactly how you have done relative to everyone else in the class.
Letter grades will be assigned as an approximation for you to judge how you are doing. The reason for the
approximation is that the actual exam score, not the letter grade, will be used in computing your final grade.
At the end of the course there will be a total of 480 points possible; 300 points from lecture, 160 from lab, and 20
points that will be determined from overall performance in lab and lecture. I will determine an average for each
person and plot those on a new histogram. Then, judging the overall quality of the grades (taken as a whole) and the
normal breaks in the histogram, I will divide the histogram into groups to assign the final letter grades.
7. Calculators: For any quiz or exam question that requires a calculator, a NON-graphing calculator must be used.
If you do not have one, there will be some you can use. If you want to use one of the department’s, you may want to
familiarize yourself with it a little before you actually need it.
8. Cell phones: Cell phones must be turned off and put away during all classes.
9. Students majoring in Adolescence Education: Biology 7-12 will focus on acquiring knowledge and developing
skills aligned with learning outcomes from the College's Conceptual Framework for Teacher Education and those
established by the National Science Teachers Association. In particular, this course addresses Conceptual Framework
Learning Outcome 2: Possess in-depth knowledge of the subject area to be taught; Conceptual Framework Learning
Outcome 13: Demonstrate sufficient technology skills and the ability to integrate technology into classroom
teaching/learning; NSTA Standard I: Content; NSTA; Standard 2: Nature of Science; NSTA Standard 3: Inquiry;
NSTA Standard 4: Issues; and NSTA Standard 7: Science in the Community.
1
CELLULAR BIOLOGY
SPRING 2011
CELL BIOLOGY
Lecture Schedule – Spring 2011
TOPICS
READINGS
I. Introductory Topics
Chapter 1
p. 1-7
p. 20-21
p. 737-752
p. 7-25
p. 25-29
1. Discovery & Characteristics of Cells
2. Size and Measurements
3. Microscopy
4. Viruses, Prokaryotes & Eukaryotes
5. Evolution of Cells
B. Organic Molecules
6. Chemical Bonds
7. Properties of Water
8. pH and Buffers
9. Hydrocarbons & Functional Groups
Chapter 2
p. 32-38
p. 38-39
p. 39-41
p. 41-43
10. Carbohydrates
11. Lipids
12. Proteins
13. Nucleic acids
p.
p.
p.
p.
p.
p.
43-49
49-51
51-69
75-77
403-407
428-432
II. Membranes
C. Membrane Structure and Function
14. Composition and Structure
15. Transport
Chapter 4
p. 122-149
p. 149-160
D. The Cell Surface
16. Receptors and Signal Transduction
Chapter 15
p. 627-643
III. Cellular Processes Involving Membranes
E. Enzymes and Metabolic Processes
18. Energy, Enzymes and Metabolic Pathways
19. Glycolysis, Mitochondria and Respiration
Ch. 3,5 & 6
p. 86-107
p. 108-114
p. 182-209
p. 217-239
20. Chloroplasts and Photosynthesis
2
CELLULAR BIOLOGY
SPRING 2011
TOPICS
READINGS
F. Internal Membranes & Synthesis of Macromolecules
21. Endoplasmic Reticulum
22. Golgi
23. Lysosomes
24. Endocytosis and Membrane Flow
IV. Cellular Processes not Involving Membranes (as much)
G. Cellular Information and Its Dissemination
25. The Nucleus and the Organization of Chromosomes
26. Transcription and Processing
27. Translation
H. Cell Division
28. DNA Replication and The Cell Cycle
Chapter 8
p. 279-299
p. 299-311
p. 311-313
p. 316-323
Ch. 12 & 11
p. 492-504
p. 439-447
p. 452-464
p. 470-484
p.
p.
I. The Cytoskeleton: Intracellular Structure and Movement
30. Microtubules
Cilia & Flagella
Intermediate Filaments and Microfilaments.
J. Specialized Cell Types
31. Nerve Cells
32. Contractile Cells
Chapter 9
p. 334-355
p. 588-603
p. 356-364
p. 364-372
p.
p.
"Long ago it became evident that the key to every
biological problem must finally be sought in the cell,
for every living organism is, or at sometime has been, a cell."
Edmund B. Wilson, 1925
3
550-558
578-581
166-173
375-381
CELLULAR BIOLOGY
SPRING 2011
CELL BIOLOGY
Laboratory Schedule – Spring 2011
PROJECT
Subject of QUIZ
or Lab Report
1. Microscopy
1
---------------
2. Nucleic Acids
3
Microscopy
Feb 10
3. pH and Buffers
HANDOUT
Nucleic Acids
Feb 17
4. Spectrophotometry
HANDOUT
pH and Buffers
Feb 24
5. Spectrophotometry of DNA and RNA
5
Spectrophoto.
Mar 3
6. Electrophoresis
6
DNA/RNA L.R.
Mar 10
7. Membrane Permeability
9 and Handout
Electrophoresis
Mar 17
SPRING BREAK
-------------------
-------------------
DATE
TOPIC
Jan 27
Feb
3
Mar 24
8. Cell Fractionation
10
Memb. Permeability
Mar 31
9. Succinate Dehydrogenase Activity
11
Cell Fractionation
Apr 7
10. Enzyme Kinetics
HANDOUT
Succin. Dehydro. L.R.
Apr 14
11. Chromatography
12
Enzyme Kinetics
Apr 21
12. Hill Reaction
13
Chromatography
Apr 28
13. Restriction Mapping of Lambda DNA
17 and Handout
Hill Reaction
May 5
TBA
Lab Reports and Quizzes: There will be two lab reports each worth 25 points, due as shown in boldface type
above. There will be 10 quizzes during the semester, one for each lab as indicated above. Each quiz will be
worth 10 points. Note that quizzes for a particular lab will be given at the beginning of the subsequent lab
period. There will be NO make-ups for missed quizzes. The quiz of anyone arriving late will be collected
no later than the last quiz handed in by someone who was present at the beginning of the class period. A
maximum of 5 quiz points will be awarded to anyone who has missed the laboratory upon which those
points are based. The lowest quiz grade will be dropped in determining the final grade for the course.
4