i th PROCEEDING OF THE 10 ANNUAL CONFERENCE OF THE ASIAN REPRODUCTIVE BIOTECHNOLOGY SOCIETY Sea-links Beach Resort Hotel, Mui Ne, Vietnam August 19 - 25, 2013 “Innovation in Biotechnology for Health, Medicine and Agriculture” Edited on behalf of the Society by Program Chair Nguyen Van Thuan, Ph.D. International University, Vietnam National University HCMC, Vietnam Tan Tao University, Ho Chi Minh City, Vietnam 2013 ARBS SCIENTIFIC STEERING COMMITTEES ARBS SCIENTIFIC STEERING COMMITTEE 2013 ORGANIZING COMMITTEE Dr. A.W.S. Chan (China) Dr. Atsuo Ogura (Japan) Dr. Bui Hong Thuy (Vietnam) Dr. Changxin Wu (China) Dr. Chong Li (China) Dr. Eimei Sato (Japan) Dr. Graham Jenkin (Australia) Dr. Hoon Taek Lee (South Korea) Dr. Hung-Chih Kuo (Taiwan) Dr. Il-keun Kong (Korea) Dr. Jin-Hoi Kim (South Korea) Dr. Ke-Huan Lu (China) Dr. Mark Nottle (Australian) Dr. Mashashi Miyake (Japan) Dr. Mongkol Techakumphu (Thailand) Dr. Nam-Hyung Kim (South Korea) Dr. Naojiro Minami (Japan) Dr. Nisar A.Wani (UAE) Dr. Noboru Manabe (Japan) Dr. Nguyen Van Thuan (Vietnam) Dr. Qing-Yuan Sun (China) Dr. Ramli Abdullah (Malaysia) Dr. Rangsun Parnpai (Thailand) Dr. Sandeep Goel (India) Dr. Satoshi Kishigami (Japan) Dr. SC Ng (Singapore) Dr. Shaorong Gao (China) Dr. Shien Zhu (China) Dr. Takashi Miyano (Japan) Dr. Takashi Nagai (Japan) Dr. Teoan Kim (South Korea) Dr. Teruhiko Wakayama (Japan) Dr. Xiuchun Cindy Tian (China) Dr. Xuan Nguyen Bui (Vietnam) H.E. Minister Nguyen Quan, Chairman Ministry of Science and Technology of Vietnam Prof. Phan Thanh Binh, Co-Chairman President Vietnam National University, HCMC, Vietnam Prof. Ho Thanh Phong, Co-Chairman Rector International University, VNU-HCMC, Vietnam Prof. Vo Tong Xuan, Co-Chairman Rector Tan Tao University, Long An, Vietnam Prof. Nguyen Van Thuan, Advisor International University, VNU-HCMC, Vietnam Tan Tao University, Long An, Vietnam Secretary-general: Ms. Vo Ngoc Quynh Nhu International University, VNU-HCMC, Vietnam Ms. Nguyen Thi Mai Tram International University, VNU-HCMC, Vietnam Secretary: Dr. Bui Chi Bao Dr. Nguyen Thi Thu Hoai Mr. Nguyen Hong Long Mr. Nguyen Thien Quang Mr. Phan Cong Hoang Mr. Ton Ngoc Bao Ms. Tran Thi Quynh Dao Ms. Tran Thi Nhu Hao Ms. Le Tran Hong Ngoc Ms. Truong Thi Bich Lieu ARBS ORGANIZING OFFICE President: Dr. Nguyen Van Thuan Website Administrator: Mr. Toan Dang Phong Secretary: Ms. Vo Ngoc Quynh Nhu Email: arbs@arbsociety.org Co-hosted by: Ministry of Science and Technology, VIETNAM International University, Vietnam National University HCMC, VIETNAM Tan Tao University, Long An, VIETNAM ii The Conference Steering Committee of The Asian Reproductive Biotechnology would like to Acknowledge and Thank the Following Organization and Companies for Their Support and Sponsor to this Conference: MINISTRY OF SCIENCE AND TECHNOLOGY OF VIETNAM, VIETNAM http://www.most.gov.vn/Desktop.aspx/Home-EN/ INTERNATIONAL UNIVERSITY, VIETNAM NATIONAL UNIVERSITY OF HO CHI MINH CITY, VIETNAM http://www.hcmiu.edu.vn/vn/ TAN TAO UNIVERSITY, LONG AN, VIETNAM http://ttu.edu.vn/index.php?language=vn 6 PRIME TECH K.K http://www.primetech-jp.com/ HAMILTON THORNE BIOSCIENCES K.K http://www.hamiltonthorne.com/ VK SCIENTIFIC EQUIPMENT CO., LTD http://vietkhoaco.blogspot.com/ SAIGON INSTRUMENTATION JOINT STOCK COMPANY http://www.sisc.com.vn/ BCE VIETNAM CO., LTD http://www.bcevietnam.com.vn/ Contents 10th Annual Conference of Asian Reproductive Biotechnology Society 2013 iii ASIAN REPRODUCTIVE BIOTECHNOLOGY SOCIETY The Tenth Annual Conference ARBS 2013 Sea-links Beach Resort Hotel, Mui Ne, Vietnam August 19 - 25, 2013 Conference Program Monday 19th August, 2013 14:00 PM – 16:00 PM Registration & Local organizing committee meeting 16:00 PM – 18:00 PM ARBS Executive Committee Meeting 18:00 PM – 20:00 PM Welcome Party for ARBS Executive Committee and previous Local Organizing Committee Tuesday 20th August, 2013 7:00 AM – 8:30 AM Registration 8:30 AM – 10:30 AM - Opening remarks and welcome by: H.E. Minister Nguyen Quan Ministry of Science and Technology, Vietnam Professor Phan Thanh Binh President Vietnam National University, HCMC, Vietnam Professor Ho Thanh Phong Rector International University, VNU-HCMC, Vietnam Professor Vo Tong Xuan Rector Tan Tao University, Long An, Vietnam - Overview of the 10 Years ARBS establishment by ARBS Chairman - Take a Photograph for all the participants Session I Session Chairs: Dr. Atsuo Ogura, Dr. Ji Wu 10:30 AM – 11:30 AM Cells into tissues: a cadherin story Dr. Masatoshi Takeichi Contents 10th Annual Conference of Asian Reproductive Biotechnology Society 2013 iv RIKEN Center for Developmental Biology, Kobe, Japan 11:30 AM – 12:10 PM Genomic reprogramming errors do not accumulate with serial recloning in the mouse Dr. Teruhiko Wakayama University of Yamanashi, Japan 12:10 PM – 13:30 PM Lunch Break Session II Session Chairs: Dr. Teruhiko Wakayama, Dr. Qing-Yuan Sun 13:30 PM – 14:00 PM In vivo and in vitro differentiation of postnatal female rat germline stem cells into oocytes Dr. Ji Wu Shanghai Jiaotong University, China 14:00 PM – 14:30 PM Research on goat cloning and stem cell for possible application in agriculture and human medicine in Malaysia Dr. Ramli Abdullah Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia 14:30 PM – 15:00 PM Mitochondrial during human oocyte maturation - aiming at mitochondrial transfer Dr. Yoshiharu Morimoto CEO, IVF Japan 15:00 PM – 15:10 PM Break Session III Session Chairs: Dr. Haruhiko Koseki, Dr. Yoshiharu Morimoto 15:10 PM – 15:40 PM Microfilaments and microtubules in oocytes maturation: current frontiers and beyond Dr. Nam-Hyung Kim Chungbuk National University, Republic of Korea 15:40 PM – 16:10 PM The role of oocyte nucleolus precursor bodies (NPBs) Dr. Helena Fulka Institute of Animal Science, Pratelstvi 815, Prague, Czech Republic 16:10 PM – 16:40 PM Identification, proliferation, and reprogramming of putative stem cell in the adult pig ovary Dr. Hong-Thuy Bui International University, Vietnam National University HCMC, Vietnam Tan Tao University, Long An, Vietnam 16:40 PM – 17:10 PM Survivin is essential for fertile egg production and female fertility in mice Contents 10th Annual Conference of Asian Reproductive Biotechnology Society 2013 v Dr. Qing-Yuan Sun Chinese Academy of Sciences, Beijing 100101, China 19:00 PM – 22:00 PM Welcome party Wednesday 21st August, 2013 Session IV Session Chairs: Dr. Masatoshi Takeichi, Dr. Mark B. Nottle 8:00 AM – 8:30 AM Status of transcription regulation in mammalian oocytes and embryos Dr. Dong Il Jin Chungnam National University, Daejeon 305-764, Korea 8:30 AM – 9:00 AM The role of enhancer of polycomb during spermiogenesis Dr. Haruhiko Koseki RIKEN Center for Integrative medical Science 9:00 AM – 9:30 AM Epigenetic reprogramming of somatic cells by nuclear transfer, chemical and viral inductions Dr. Xiuchun (Cindy)Tian Center for Regenerative Biology, University of Connecticut, United States 9:30 AM – 10:00 AM Recent developments in assisted reproduction in camels Dr. Nisar Wani Camel Reproductive Center Dubai, UAE 10:00 AM – 10:10 AM Break Session V Session Chairs: Dr. Nguyen Van Thuan, Dr. Xiuchun Cindy Tian 10:10 AM – 10:40 AM Influence of acupuncture stimulation on pregancy rates for women undergoing embryo transfer at infertility department, Hung Vuong hospital Dr. Ly Thai Loc Hung Vuong Hospital, Ho Chi Minh City, Vietnam 10:40 AM – 11:05 AM Results of modified protocols in in-vitro maturation (IVM) of oocytes Dr. Ho Manh Tuong Research Center for Genetics and Reproductive Health (CGRH), School of Medicine, Vietnam 11:05 AM – 11:30 AM Growth retardation in human blastocysts decreases their implantation potential possibly due to an increase of the incidence Contents 10th Annual Conference of Asian Reproductive Biotechnology Society 2013 vi of abnormal spindles Dr. Shu Hashimoto IVF Namba Clinic, Nishiku, Osaka, Japan 11:30 AM – 11:55 AM Isolation of porcine embryonic stem cells from heterozygous parthenogenetic embryos Dr. Mark B. Nottle University of Adelaide, Adelaide, Australia 11:55 AM – 13:00 PM Lunch Break Session VI Session Chairs: Dr. Satoshi Kishigami, Dr. Mukesh Gupta 13:00 PM – 13:30 PM Generation of mammalian haploid embryonic stem cells Dr. Jinsong Li Shanghai Institutes for Biological Sciences Chinese Academy of Sciences, China 13:30 PM – 14:00 PM Cord lining stem cells – a novel stem cell platform for tissue regeneration with promising commercial potential in the sphere of regenerative medicine Dr. Thang Toan Phan Department of Surgery, Yong Loo Lin School of Medicine, National University of Singapore Cell Research Corp, Singapore Session VII Poster session (with break) 14:05 PM – 16:30 PM Poster session 16:30 PM – 18:30 PM Demonstration of ICSI, SCNT, Embryonic manipulation Thursday 22nd August, 2013 Session VIII Session Chairs: Dr. Teoan Kim, Dr. Phan Toan Thang 8:00 AM – 8:30 AM Inter-species and inter-generic somatic cell nuclear transfer: challenging for conservation endangered species Dr. Rangsun Parnpai Suranaree University of Technology, Thailand 8:30 AM – 9:00 AM Reprogramming events in somatic cells induced by extract from germinal vesicle (GV) stage pig oocytes Dr. Nguyen Van Thuan Department of Animal Biotechnology, Konkuk University, Korea 9:00 AM – 9:30 AM Stem cells research and application in Vietnam, results and Contents vii 10th Annual Conference of Asian Reproductive Biotechnology Society 2013 perspectives Dr. Bui Xuan Nguyen Institute of Biotechnology, Vietnam 9:30 AM – 9:40 AM Break Session IX Session Chairs: Dr. Nam-Hyung Kim, Dr. Bui Xuan Nguyen 9:40 AM – 10:05 AM Sperm: not just a carrier of father’s DNA Dr. Mukesh Gupta Department of Animal Biotechnology, Konkuk University, Korea 10:05 AM – 10:30 AM Porcine blastocyst vitrification after artificial shrinkage of the blastocoele Dr. Marlon B. Ocampo Reproductive Biotechnology Unit, Philippines 10:30 AM – 10:55 AM Recent advances in asymmetric division Dr. Shao-Chen Sun the molecular mechanism of oocyte College of Animal Science and Technology, China Session X Session Chairs: Dr. Ke-Huan Lu, Dr. Rangsun Parnpai 10:55 AM – 11:20 AM Hypoosmotic swelling test to assess the functional membrane integrity of water buffalo spermatozoa Dr. Danilda –Hufana Duran Reproductive Biotechnology Laboratory, Philippines 11:20 AM – 11:45 AM Reproductive effects of two neonicotinoid insecticides on mouse sperm function and early embryonic development in vitro Dr. Li Yan Reproductive Medical Center, China 11:45 AM – 12:10 PM Follicular dynamics during ovum pick-up at different intervals in water buffalo Dr. Peregrino G. Duran Reproductive Biotechnology Laboratory, Philippines 12:10 PM – 13:30 PM Session XI 13:30 PM – 13:50 PM Lunch Break Session Chairs: Dr. Jinsong Li, Dr. Ramli B. Abdullah Determination of hybrid individuals between domestic female cow (bos taurus) and wild male gaur (bos gaurus) in national park of Phuoc Binh - adjointive forests of Lam Dong-Ninh Thuan-Khanh Hoa Contents 10th Annual Conference of Asian Reproductive Biotechnology Society 2013 viii Dr. Le Xuan Tham Department of Science and Technology, Vietnam 13:50 PM – 14:10 PM In vitro tert mRNA expression in relation to chromosomal stability, senescence and telomere length and SCNT cloned embryo production in bubalus bubalis Dr. Neelam Gupta Transgenic Research Laboratory, India 14:10 PM – 14:30 PM Effect of space environment on mammalian reproduction (space pup) Dr. Sayaka Wakayama Center for Developmental Biology (CDB), RIKEN, Japan Session XII Session Chairs: Dr. Dong Il Jin, Dr. Chong Li 14:30 PM – 14:50 PM The roles of tet genes in mouse early embryo development and pig induced pluripotent stem cells Dr. Ziyi Li Jilin University, China 14:50 PM – 15:10 PM Vitrification of in-vitro matured goat (capra hircus) oocytes using cryotop method optimized with cytochalasin-b Dr. Eufrocina P. Atabay Reproductive Biotechnology Unit, Philippines Session XIII Session Chairs: Dr. Ho Manh Tuong, Dr. Nisar Ahmad Wani 15:10 PM – 15:30 PM Establish MESC lines using long-term preserved mouse sperm at refrigerator temperature Dr. Chong Li Advanced Institute of Translational Medicine, Tongji University, China 15:30 PM – 15:50 PM Optimized sequential culture system for the production of bovine (bos taurus) embryos in-vitro Dr. Edwin C. Atabay Philippine Carabao Center at CLSU, Science City of Munoz, Philippines 15:50 PM – 16:10 PM Cryopreservation of immature and in vitro matured goat oocytes by the cryoloop vitrification method Dr. Guo Bo Quan Yunnan Animal Science and Veterinary Institute, Jindian, Yunnan Province, China Session XIV Closing Ceremony Session Chairs: Dr. Ho Thanh Phong, Dr. Vo Tong Xuan & Dr. Nguyen Van Thuan 16:10 PM – 16:30 PM Award for Poster Presentation Contents 10th Annual Conference of Asian Reproductive Biotechnology Society 2013 16:30 PM – 16:40 PM Introduce the hosted country for the 11th ARBS meeting 16:40 PM – 17:00 PM Closing Ceremony by Local Organizing Committee 18:30 PM – 22:00 PM Farewell Party ix Friday 23 – Saturday 24 August, 2013: Post-conference Scientific Trip Contents 10th Annual Conference of Asian Reproductive Biotechnology Society 2013 x PROCEEDINGS OF THE 10TH ANNUAL CONFERENCE OF THE ASIAN REPRODUCTIVE BIOTECHNOLOGY SOCIETY Sea-links Beach Resort, Mui Ne, Vietnam, August 19 - 25, 2013 PLATFORM SESSION Cells into tissues: a cadherin story Masatoshi Takeichi 1 Genomic reprogramming errors do not accumulate with serial recloning in the mouse Teruhiko Wakayama 2 In vivo and in vitro differentiation of postnatal female rat germline stem cells into oocytes Ji Wu 3 Research on goat cloning and stem cell for possible application in agriculture and human medicine in Malaysia Ramli Abdullah 4 Mitochondrial during human oocyte maturation - aiming at mitochondrial transfer Yoshiharu Morimoto 5 Microfilaments and microtubules in oocytes maturation: current frontiers and beyond Nam-Hyung Kim 6 RIKEN Center for Developmental Biology, Kobe, Japan University of Yamanashi, Japan Shanghai Jiaotong University, China Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia CEO, IVF Japan Chungbuk National University, Republic of Korea The role of oocyte nucleolus precursor bodies (NPBs) Helena Fulka 7 Institute of Animal Science, Pratelstvi 815, Prague, Czech Republic Identification, proliferation, and reprogramming of putative stem cell in the adult pig ovary Hong-Thuy Bui 8 Survivin is essential for fertile egg production and female fertility in mice Qing-Yuan Sun 9 Status of transcription regulation in mammalian oocytes and embryos Dong Il Jin 10 The role of enhancer of polycomb during spermiogenesis Haruhiko Koseki 11 Epigenetic reprogramming of somatic cells by nuclear transfer, chemical and viral inductions Xiuchun (Cindy)Tian 12 International University, Vietnam Tan Tao University, Long An, Vietnam Chinese Academy of Sciences, Beijing 100101, China Chungnam National University, Daejeon 305-764, Korea RIKEN Center for Integrative medical Science Center for Regenerative Biology, University of Connecticut, United States Contents 10th Annual Conference of Asian Reproductive Biotechnology Society 2013 xi Recent developments in assisted reproduction in camels Nisar Wani 13 Influence of acupuncture stimulation on pregancy rates for women undergoing embryo transfer at infertility department, Hung Vuong hospital Ly Thai Loc 14 Camel Reproductive Center Dubai, UAE Hung Vuong Hospital, Ho Chi Minh City, Vietnam Results of modified protocols in in-vitro maturation (IVM) of oocytes Ho Manh Tuong 15 Growth retardation in human blastocysts decreases their implantation potential possibly due to an increase of the incidence of abnormal spindles Shu Hashimoto 16 Isolation of porcine embryonic stem cells from heterozygous parthenogenetic embryos Mark B. Nottle 17 Generation of mammalian haploid embryonic stem cells Jinsong Li 18 Cord lining stem cells – a novel stem cell platform for tissue regeneration with promising commercial potential in the sphere of regenerative medicine Thang Toan Phan 19 Inter-species and inter-generic somatic cell nuclear transfer: challenging for conservation endangered species Rangsun Parnpai 20 Induced pluripotent stem cell by GV oocytes extract: gvIPS Cells Nguyen Van Thuan 21 Stem cells research and application in Vietnam, results and perspectives Bui Xuan Nguyen 22 Sperm: not just a carrier of father’s DNA Mukesh Gupta 23 Porcine blastocyst vitrification after artificial shrinkage of the blastocoele Marlon B. Ocampo 24 Recent advances in the molecular mechanism of oocyte asymmetric division Shao-Chen Sun 25 Research Center for Genetics and Reproductive Health (CGRH), School of Medicine, Vietnam IVF Namba Clinic, Nishiku, Osaka, Japan University of Adelaide, Adelaide, Australia Shanghai Institutes for Biological Sciences Chinese Academy of Sciences, China Department of Surgery, Yong Loo Lin School of Medicine, National University of Singapore Cell Research Corp, Singapore Suranaree University of Technology, Thailand International University, Vietnam National University HCMC, Vietnam TanTao University, Long An, Vietnam Institute of Biotechnology, Ha Noi, Vietnam Department of Animal Biotechnology, Konkuk University, Korea Reproductive Biotechnology Unit, Philippines College of Animal Science and Technology, China Contents 10th Annual Conference of Asian Reproductive Biotechnology Society 2013 xii Hypoosmotic swelling test to assess the functional membrane integrity of water buffalo spermatozoa Danilda –Hufana Duran 26 Reproductive effects of two neonicotinoid insecticides on mouse sperm function and early embryonic development in vitro Li Yan 27 Follicular dynamics during ovum pick-up at different intervals in water buffalo Peregrino G. Duran 28 Determination of hybrid individuals between domestic female cow (bos taurus) and wild male gaur (bos gaurus) in national park of Phuoc Binh - adjointive forests of Lam Dong-Ninh Thuan-Khanh Hoa Le Xuan Tham 29 In vitro tert mRNA expression in relation to chromosomal stability, senescence and telomere length and SCNT cloned embryo production in bubalus bubalis Neelam Gupta 30 Effect of space environment on mammalian reproduction (space pup) Sayaka Wakayama 31 The roles of tet genes in mouse early embryo development and pig induced pluripotent stem cells Ziyi Li 32 Vitrification of in-vitro matured goat (capra hircus) oocytes using cryotop method optimized with cytochalasin-b Eufrocina P. Atabay 33 Establish MESC lines using long-term preserved mouse sperm at refrigerator temperature Chong Li 34 Optimized sequential culture system for the production of bovine (bos taurus) embryos in-vitro Edwin C. Atabay 35 Cryopreservation of immature and in vitro matured goat oocytes by the cryoloop vitrification method Guo Bo Quan 36 Reproductive Biotechnology Laboratory, Philippines Reproductive Medical Center, China Reproductive Biotechnology Laboratory, Philippines Department of Science and Technology, Vietnam Transgenic Research Laboratory, India Center for Developmental Biology (CDB), RIKEN, Japan Jilin University, China Reproductive Biotechnology Unit, Philippines Advanced Institute of Translational Medicine, Tongji University, China Philippine Carabao Center at CLSU, Science City of Munoz, Philippines Yunnan Animal Science and Veterinary Institute, Jindian, Yunnan Province, China Contents 10th Annual Conference of Asian Reproductive Biotechnology Society 2013 xiii POSTER SESSION 1. SGO1 maintains bovine meiotic centromeric cohesions of sister chromatids and directly affects embryo development Feng-xia Yin and Guang-peng Li* 37 The Key Laboratory of National Education Ministry for Mammalian Reproductive Biology and Biotechnology, Inner Mongolia University, Inner Mongolia, Hohhot, China 2. Development patterns of water buffalo (bubalus bubalis) IVEP embryos at various age 38 and stages Prudencio B. Pedro*, Marlon B. Ocampo, Eufrocina P. Atabay, Flocerfida P. Aquino, Lerma C. Ocampo, Excel Rio Maylem, and Edwin C. Atabay Reproductive Biotechnology Laboratory, Office of the Executive Director, Philippine Carabao Center, National Headquarters and Gene Pool, Science city of Munoz 3120, Nueva Ecija, Philippines 3. Toxicity and protective efficiency of cryoprotectants to catfish (pangasidae hypophthamus) embryos stored at low temperature Nguyen Thi Hong1*, Nguyen Thi Nhung1, Nguyen Thi Uoc1, Nguyen Thi Hiep1, Nguyen Viet Linh1, Nguyen Viet Hanh1, Pham Van Khanh2, Ha Thi Ngoc Nga2, Tran Van Thuong3, and Bui Xuan Nguyen1* 39 Effect of immunomodulators on reproductive performance of sahiwal cows Anjali Aggarwal* and Gulab Chandra 40 Can glucose and oxygen levels affect the development and sex ratio in mouse embryo culture? Mai Tram Nguyen1,2*, Ji Yeon Cailyn Kim2, and Zhen Xiang2 41 1) Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, Vietnam; 2)Research Institute for Aquaculture No.2, Ho Chi Minh City, Vietnam; 3)Research Institute for Aquaculture No.1, Vietnam 4. Dairy Cattle Physiology Division, National Dairy Research Institute, Karnal-132001 (Haryana), India 5. 1) Department of Biotechnology, School of Biotechnology, International University, Vietnam National University, Ho Chi Minh City, Vietnam; 2)Education Program in Reproduction and Development (EPRD), Department of Obstetrics and Gynaecology, Monash University, Clayton, Australia 6. The effectiveness of hyaluronic acid versus polyvinylpyrrolidone in intracytoplasmic 42 sperm injection Kha TH Le1,2*, Quynh N Nguyen1,2, Duy H Nguyen1,2, Khang A Nguyen2, Mai T Nguyen1, Bich N Nguyen1,2, Lian S Liow2, and Vinh Q Dang1,2 1) IVFVH , Van Hanh General Hospital, Vietnam; 2)Research Center for Genetics and Reproductive Health (CGRH), School of Medicine, Vietnam National University - Ho Chi Minh City, Vietnam 7. Live-cell imaging analysis of mouse oocytes injected with freeze-dried spermatozoa Eiji Mizutani*, Sayaka Wakayama, Kana Kishida, and Teruhiko Wakayama 43 Effects of taxol in cryopreservation of mature bovine oocytes by vitrification Nguyen Thi Thuong Huyen1, 2*, Le Thanh Long3, Nguyen Quoc Dat4, Hoang Nghia Son3, and Phan Kim Ngoc1 44 Faculty of Life and Environmental Sciences, University of Yamanashi, Kofu, Japan 8. 1) University of Science, Vietnam National University, Ho Chi Minh City, Vietnam; 2)Department of Animal Physiology, Faculty of Biology, University of Pedagogy, Ho Chi Minh City, Vietnam; 3)Institute of Tropical Biology, VAST, Vietnam; 4)National Institute of Animal Husbandry, Vietnam Contents 9. 10th Annual Conference of Asian Reproductive Biotechnology Society 2013 xiv Measurement reactive oxygen species (ROS) levels in neat semen of 600 infertile couples 45 Huynh Thi Hong Vinh1*, Nguyen Ngoc Yen Nhi1, Tran Tu Cam1, Nguyen Thi Lien Thi1, Nguyen Thi Mai1, and Ho Manh Tuong1,2 1) Ngoc Lan Clinic, Ho Chi Minh City, Vietnam; 2)Research Center for Genetics and Reproductive Health (CGRH), School of Medicine, Vietnam National University - Ho Chi Minh City, Vietnam 10. Preimplantation genetic diagnosis on day 3 human embryos Lan TT Nguyen1,2*, Hoang VM Bui3, Binh TT Truong1, Thuc N Vo2, Vinh Q Dang2, Tuong M Ho1,2, and Kiet D Truong3 46 1) IVFAS, An Sinh General Hospital, Ho Chi Minh City, Vietnam; 2)Research Center for Genetics and Reproductive Health (CGRH), School of Medicine, Vietnam National University - Ho Chi Minh City, Vietnam; 3)University of Medicine and Pharmacy, Ho Chi Minh City, Vietnam 11. Development of non-surgical embryo transfer technique for production of transgenic cow 47 Sang Jun Uhm1*, Jung Seok Yang1, Young-Tae Heo2, Yong-Nan Xu2, Bon-Chul Koo3, Nam-Hyung Kim2, and Dae Hwan Ko1 1) Department of Animal Science and Biotechnology, Sangji Youngseo College, Wonju, South Korea; 2)Department of Animal Science, Chungbuk National University, Cheongju, South Korea; 3)Department of Physiology, Catholic University of Daegu School of Medicine, Daegu, South Korea. 12. Effect of application modified IMSI in assisted reproduction Bao G Huynh1,2*, Anh H Le1,2, Xuan T Pham1, Nghia T Luong2, Tuan A Lam1,2, and Tuong M Ho1,2 48 1) IVF An Sinh, An Sinh General Hospital, Ho Chi Minh City, Vietnam; 2)Research Center for Genetics and Reproductive Health (CGRH), School of Medicine, Vietnam National University - Ho Chi Minh City, Vietnam 13. Rho-GTPase effector rock regulates actin-mediated cytokinesis in mouse oocyte 49 Xing Duan1, Zhen-Bo Wang3, Xiang-Shun Cui2, Nam-Hyung Kim2, Qing-Yuan Sun3, and Shao-Chen Sun1* 1) Nanjing Agricultural University, Nanjing, China; 2)Chungbuk National University, Cheongju, Republic of Korea; University of Chinese Academy of Sciences, Beijing, China 3) 14. Artifical oocyte activation by using calcium ionophore a23187 in severe teratozoospermia patients Lan TT Nguyen1* and Tuong M Ho1,2 50 1) IVF An Sinh, An Sinh General Hospital, Ho Chi Minh City, Vietnam; 2)Research Center for Genetics and Reproductive Health (CGRH), School of Medicine, Vietnam National University - Ho Chi Minh City, Vietnam 15. Vitrification of transgenic bovine embryos by PNC container 51 Dae Hwan Ko*1, Jung Seok Yang1, Joon Gyo Lim2, Young-Tae Heo2, Yong-Nan Xu2, Bon-Chul Koo3, Nam-Hyung Kim2, and Sang Jun Uhm1 1) Department of Animal Science and Biotechnology, Sangji Youngseo College, Wonju, South Korea; 2)Department of Animal Science, Chungbuk National University, Cheongju, South Korea; 3)Department of Physiology, Catholic University of Daegu School of Medicine, Daegu, South Korea. 16. Comparison of 5% and ambient oxygen during days 1-3 of in vitro culture of human embryos An Vo* 52 Hanh Phuc Hospital, Ho Chi Minh City, Vietnam 17. Ethanol sclerotherapy after ultrasound-guided aspiration can prolong the time to recurrence 53 of endometrial cyst Yoshiharu Morimoto, Shu Hashimoto, Yoshiharu Nakaoka, Tomoko Inoue, Takao Himeno, Yoko Ohnishi, Rie Nakahira, and Keijiro Ito* Medical Department, IVF Namba Clinic, Nishiku, Osaka, Japan Contents 10th Annual Conference of Asian Reproductive Biotechnology Society 2013 xv 18. Mycotoxins containing diet decreases oocyte quality in mouse Cheng-Cheng Zhu1, Ziang-Shun Cui2, Nam-Hyung Kim2, and Shao-Chen Sun1* 54 1) College of Animal Science and Technology, Nanjing Agricultural University, Jiangsu, Nanjing, China; Department of Animal Science, Chungbuk National University, Chungbuk, Cheongju, Republic of Korea 2) 19. The correlation between the morphological characters of the oocyte and the ICSI outcomes Dung TP Nguyen1*, Lan TT Nguyen1,2, Xuan T Pham2, and Tuong M Ho1,2 55 1) IVF An Sinh, An Sinh General Hospital, Ho Chi Minh City, Vietnam Research Center for Genetics and Reproductive Health (CGRH), School of Medicine, Vietnam National University - Ho Chi Minh City, Vietnam 2) 20. Viability of extended goat semen stored at refrigerated condition Flocerfida P. Aquino1*, Krystel Grace B. Vergara2, Lerma C. Ocampo1, and Eufrocina P. Atabay1 56 1) Reproductive Biotechnology Unit, Philippine Carabao Center, Science City of Munoz, Nueva Ecija, Phillipines, Central Luzon State University, Science City of Munoz, Nueva Ecija, Philippines 2) 21. The maturation rate and ICSI outcomes of different morphological classifications of germinal vesicle (gv) oocytes Lan TT Nguyen1,2*, Xuan T Pham1, and Lan TN Vuong1,3 57 22. Effect of varying sperm concentration on the post-thaw liveability of frozen goat semen Lerma C. Ocampo*1, Flocerfida P. Aquino1, Edeneil Jerome P. Valete2, Marlon B. Ocampo1,2, and Eufrocina P. Atabay1 58 1) IVF An Sinh, An Sinh General Hospital, Ho Chi Minh City, Vietnam; 2)Research Center for Genetics and Reproductive Health (CGRH), School of Medicine, Vietnam National University - Ho Chi Minh City, Vietnam; 3) University of Medicine and Pharmacy, Ho Chi Minh City, Vietnam 1) Philippine Carabao Center–Reproductive Biotechnology Unit at National Headquarters and Genepool, Science City of Munoz, Nueva Ecija, Philippines; 2) Central Luzon State University- Institute of Graduate Studies, Science City of Munoz, Nueva Ecija, Philippines 23. Effect of medium with GM – CSF on repeated implantation failure in assisted reproduction Bao G Huynh1,2*, Lan TT Nguyen1,2, Tram TB Le2, and Vinh Q Dang1,2 59 24. Ensuring the quality of bovine embryos produced in-vitro through the inner cell mass and trophectoderm ratio ER. S. Maylem*, E. P. Atabay, L. C. Ocampo, M. B. Ocampo, P. B. Pedro, and F. P. Aquino 60 1) IVF An Sinh, An Sinh General Hospital, Ho Chi Minh City, Vietnam; 2)Research Center for Genetics and Reproductive Health (CGRH), School of Medicine, Vietnam National University - Ho Chi Minh City, Vietnam Reproductive Biotechnology Unit, Philippine Carabao Center, National Headquarters, Science City of Muñoz, Nueva Ecija, Philippines 25. Critical role of mitochondrial activity in the porcine oocyte in vitro maturation Lee Seul Ki, Zhao Minghui, Kwon Jung-Woo, Li Yinghua, Lin Zili, Jin Yongxun, Kim Nam-Hyung, and Cui Xiangshun* 61 Department of Animal Sciences, Chungbuk National University, South Korea 26. Effect of extra-nucleolus injection on development of icsi and SCNT embryos Hirohisa Kyogoku1,2*, Teruhiko Wakayama2,3, and Takashi Miyano1 1) Graduate School ofAgricultural Science, Kobe University, Kobe 657-8501, Japan; 2)Laboratory for Genomic Reprogramming, Center for Developmental Biology, RIKEN, Kobe 650-0047, Japan; 3)Faculty of Life and Environmental Sciences, University of Yamanashi, Kofu 400-8501, Japan 62 Contents 10th Annual Conference of Asian Reproductive Biotechnology Society 2013 xvi 27. Greatwall kinase is required for meiotic maturation in porcine oocytes Ying-Hua Li1, Hyoen Kang2, Yong-Nan Xu1, Young- Tae Heo1, Xiang-Shun Cui1, Nam-Hyung Kim1*, and Jeong Su Oh2*. 63 28. Similar but distinct in vitro aging phenotypes of mouse SCNT oocytes Satoshi Kishigami*, Ah Reum Lee, Thanh Ha Le, and Yoshihiko Hosoi 64 1) Deparment of Animal Science, Chungbuk National University, Chungbuk, Cheongju, Republic of Korea, 2)Genetic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University, Gyeonggi-do, Suwon, Korea. Department of Biology-Oriented Science and Technology, Kinki University, Wakayama, Kinokawa, Japan 29. Production of transgenic cynomolgus monkey embryos using interspecies somatic cell nuclear transfer Yazaki Satoko1, Oishi Takatsugu1, Inoue Kimiko2, Ogura Atsuo2, and Sankai Tadashi3* 65 1) Deparment of Advanced Technology Development team, PRIME TECH LTD, Ibaraki-ken, Tshuchiura-shi, Japan, RIKEN BioResource Center, Ibaraki-ken, Tsukuba-shi, Japan; 3)Tsukuba Primate Center for Medical Science, National Institute of Infectious Diseases, Ibaraki-ken, Tsukuba-shi, Japan 2) 30. Production of cloned mice from a drop of periheral blood Satoshi Kamimura*, Kimiko Inoue, Narumi Ogonuki, Michiko Hirose, Mami Oikawa, Masahiro Yo, Osamu Ohara, Hiroyuki Miyoshi, and Atsuo Ogura 66 RIKEN BioResource Center, Ibaraki, Tsukuba, Japan 31. Proliferation rate of ear skin fibroblast cells derived from brakmas cattle of different ages 67 Habsah Bidin1*, Najlaa Zulhemay1, Kwong Phek Jin2, Ahmad Johari1, Ajis Hassan1, Norjahan Banu Alitheen3, Nadiah Abu3, and Musaddin Kamaruddin4 1) National Animal Embryo Centre (NAEC), MARDI Kluang Research Station, Locked Bag 525, 86009 Kluang Johore, Malaysia; 2)Department of Agriculture and Food Science, Faculty of Science, University Tunku Abdul Rahman, 31900 Kampar, Perak, Malaysia; 3)Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia, 43400 Serdang, Selangor, Malaysia; 4)Strategic Livestock Research Centre, MARDI HQ, P.O. Box 12301, 50774; Kuala Lumpur, Malaysia 32. Generation of induced pluripotent stem cells from goat fibroblasts Hui Song and Feng Wang* 68 33. Next generation sequencing target to human breast cancer stem cells Diem-My Vu, Minh Nguyen Anh Ho, and Chi-Bao Bui* 69 34. Stage specific colony formation in vitro cultured spermatogonia derived from porcine, murine and canine testes Kyung Hoon Lee, Won Yong Lee, Ran Lee, Hee Chan Kim, and Hyuk Song* 70 35. Goat oocytes from lopu versus abattoir after ear fibroblast cells injection gives better SCNT performance Asdiana, A., W.E. Wan Khadijah*, and R.B. Abdullah 71 36. A zinc dependent regulatory mechanism controlling meiotic cell cycle Zhao Minghui1, Li Yinghua1, Lin Zili1, Oh Jeong-Su2, Kim Nam-Hyung1, and Cui Xiangshun1* 72 Center of Animal Embryo Engineering and Technology, Nanjing Agricultural University, Nanjing 210095, China University of Medicine and Pharmacy HCMC, Ho Chi Minh, Vietnam Department of Animal and Food Bioscience, College of Biomedical and Health Science, Konkuk University, Chung-ju, Republic of Korea Animal Biotechnology-Embryo Laboratory (ABEL), Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia 1) Department of Animal Science Chungbuk National University,Cheongju, 361-763, Korea; 2)Department of Genetic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University, Suwon, Contents 10th Annual Conference of Asian Reproductive Biotechnology Society 2013 xvii Gyeonggi-do, 440-746, Korea 37. Coexpression of hemagglutinin, neuraminidase and M1 matrix proteins in CHO-K1 cell line Tin C. Pham1*, Thuan D. Lao2, Thu T. N. Le3, and Si M. Do4 73 38. Ameliorative effect of zinc to lead exposed goat kids on lymphocyte proliferation and superoxide dismutase (SOD) expression Harjit Kaur1*, Muneendra Kumar2, Neelam Gupta1, Veena Mani3, Neelam J. Kewalramani3, and B.T. Phondba4 74 39. The expressions of GATA-4 and GFRA-1 in mouse seminiferous epithelium Guan-yu Che, Yi-xue Sun, Ai-bing Wang, Zi-yi Li, and Xueming Zhang 75 40. First report on the virulence of some Vibrio Cholerae strains isolated in Vietnam recently Le Thi Thanh Thao1*, Le Tran Hong Ngoc1, Nguyen Thi Thu Hoai1, and Pham Hung Van2 76 41. Expression and roles of bone morphogenetic protein 15 and growth differentiation factor 9 in porcine oocytes maturing in vitro Zi-Li Lin, Xiang-Shun Cui, Nam-Hyung Kim 77 1) Department of Biotechnology, School of Biotechnology, International University, Vietnam National University, Ho Chi Minh City, Vietnam; 2)School of Biotechnology, Open University of Ho Chi Minh City, Ho Chi Minh City, Vietnam; 3)School of Biotechnology, University of Industry of Ho Chi Minh City, Ho Chi Minh City, Vietnam; 4) Department of Biotechnology, University of Sciences, Vietnam National University, Ho Chi Minh City, Vietnam 1) Indian Council of Agricultural Research, New Delhi, 110001, India, 2)College of Veterinary Science and Animal Husbandry, DUVASU, Mathura, 281001, India, 3)National Dairy Research Institute, Karnal, 132001, India, 4)National Dairy Development Board, Anand, 388001, India Jilin Province Key Laboratory of Animal Embryo Engineering, and the Center for Animal Embryo Engineering of Jilin Province, College of Veterinary Medicine, Jilin University, Changchun 130062, China 1) Department of Biotechnology, School of Biotechnology, International University, Vietnam National University, Ho Chi Minh City, Vietnam, 2)NamKhoaBiotek company, Ho Chi Minh City, Vietnam Deparment of Animal Science, Chungbuk National University, Chungbuk, Cheongju, Republic of Korea 42. Roles of actin capping proteins (CAPZ) in mammalian oocyte maturation Yu-Jin Jo, Suk Namgoong, Wooin Yang, and Nam-Hyung Kim 78 Deparment of Animal Science, Chungbuk National University, Chungbuk, Cheongju, Republic of Korea 43. Generation of five human lactoferrin transgenic cloned goats using fibroblast cells and their methylation status of putative IGF2R and H19 imprinted genes Li Meng, Yongjie Wan, and Feng Wang* 79 44. Examination of the relationship between the mutation RS3741378 of SIPA1 gene and breast cancer in Vietnamese women Le Ngoc Yen*, Nguyen Dieu Hoai Chan, Le Tran Hong Ngoc, and Nguyen Thi Hue 80 45. Generation of transgenic chickens producing HGH for oral administration Bon Chul Koo, Mo Sun Kwon, Sang A Kim, Hae Jin Son, and Teoan Kim 81 46. Localization of the DIPEPTIDYL PEPTIDASE-4 gene in porcine COCS and pre-implantation embryos Ji-Hyun Lim, Hak-Jae Chung, Byoung-Chul Yang, Kyoung-Woon Kim, Sun-Young Back, Won-Gil Lee, Sung-Ku Hong, Woon-Kyoung Chang, Jin-Ki Park, and Mi-Ryung Park* 82 Center of Animal Embryo Engineering and Technology, Nanjing Agricultural University, Nanjing 210095, China Department of Biotechnology, School of Biotechnology, International University, Vietnam National University, Ho Chi Minh City, Vietnam Department of Physiology, Catholic University of Daegu School of Medicine, Daegu, Korea Contents 10th Annual Conference of Asian Reproductive Biotechnology Society 2013 xviii Animal Biotechnology, National Institute of Animal Science, Gyeonggi-do, Suwon, Republic of Korea 47. Effects of serum starvation, roscovitine treatment and contact inhibition on the cell-cycle synchronization of new-born bama mini-pig transgenic kidney fibroblast cells and the development of transgenic clone embryo Linlin Hu, Juan Lou, Xiangxing Zhu, Shouneng Quan, Shengsheng Lu*, and Kehuan Lu* 83 48. Sex reversal (XY, female) in cloned dog Hwang Kyu-Chan1, Kim Joung-Joo1, Jung Jong-Hun1, Ko Kyeong-Hee1, Jeong Yeon-Woo1, Kim Nam-Hyung2, Moon Changjong3, Shin Taeyoung1, and Hwang Woo-Suk1* 84 49. Comparison of gene expression profiles of cumulus cell of in vivo ovulated oocytes with in vitro matured oocytes Su-Jin Cho1,2,Yu-Gon Kim1, Dong-Hoon Kim3, Jae-Gyu Ryoo3, Jin-Ki Park3, and Il-Keun Kong1,2* 85 50. Establishment of effective knockin-platform using TALENS Hwang Kyu-Chan1, Jung Jong-Hun1, Ko Kyeong-Hee1, Kim Sun1, Song Seul-Ki1, Yong Hwan-Yul1, Kim Nam-Hyung2, Shin Taeyoung1, and Hwang Woo-Suk1* 86 51. The freeze-drying of antibody-conjugated gold nanoparticles for immunodiagnostics of malaria Nhu Ngoc Quynh Vo1,2*, Masuda N. Khursheid2, Sakandar Rauf2*, and Krassen Dimitrov2 87 52. The significance of chlamydia trachomatis IgG antibodies in the prediction for patients with oviduct inflammatory disease Guo Yanjun*, Ye Yang, Xu Ying, and Zhang Hongxia 88 Animal Reproduction Institute, Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Guangxi 530004, China 1) SooAm Biotech Research Foundation, Kyunginro 64, Guro-gu, Seoul, Korea; 2)Department of Animal Sciences, Chungbuk National University, Cheongju, Korea; 3)College of Veterinary Medicine and Animal Medical Institute, Chonnam National University, Gwangju, Korea 1) Department of Animal Science, Division of Applied Life Science, Gyeongsang National University, Jinju 660-701, Gyeongsangnam Province, Republic of Korea; 2)Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 660-701, Gyeongsangnam Province, Republic of Korea; 3) Animal Biotechnology Division, National Institute of Animal Science, Suwon 441-706, Gyeonggi Province, Republic of Korea 1) SooAm Biotech Research Foundation, Kyunginro 64, Guro-gu, Seoul, Korea; 2)Department of Animal Sciences, Chungbuk National University, Cheongju, Korea 1) Department of Biotechnology, School of Biotechnology, International University, Vietnam National University, Ho Chi Minh City, Vietnam, 2)School of Chemistry and Molecular Biosciences, The University of Queensland, St. Lucia, Queensland, Australia, 3)Australian Institute for Bioengineering and Nanotechnology (AIBN) Corner College and Cooper Roads (Bldg 75), The University of Queensland, St Lucia, Queensland, Australia 1) Department of Anatomy and Histology, Embriology, Medical College, Jiaxing, Zhejiang, China; Department of Clinical Medicine, Medical College, Jiaxing, Zhejiang, China; 3)Department of Histology, Embriology, Medical College, Jiaxing, Zhejiang, China; 4)Department of Human Reproduction Center, Maternity and Child Care Hospital of Jiaxing, Jiaxing, Zhejiang, China 2) 53. Nitrate removal in a recirculating aquaculture system using a biological hybrid configuration of free water surface constructed wetland and facultative anaerobic biofilter with subsurface horizontal flow Tran Cong Trung1* and Petch Pengchai2 1) Quang Binh University, Quang Binh, Vietnam; 2)Environmental Engineering, Engineering, Mahasarakham University, Mahasarakham, Thailand 89 Contents 10th Annual Conference of Asian Reproductive Biotechnology Society 2013 xix 54. The rate of growth of tialpia (Oreochromis Niloticus Peters) reared in aquaria fed with compounded fish diet out of holothuria rigida selenka and ipomea aquatic torks Serapion N. Tanduyan* 90 Cebu Technological University 6050 San Francisco, Cebu, Philippines 55. Real-time monitoring of wine fermentation using disposable, interdigitated gold microelectrodes Yen T. K. Dang1* and Loc T. Nguyen2 91 1) School of Biotechnology, International University, Vietnam National University, Ho Chi Minh City, Vietnam Department of Food Technology, School of Biotechnology, International University, Vietnam National University, Ho Chi Minh City, Vietnam 2) 56. Optimization of culture conditions for cellulase production on bagasse by anaerobic bacteria Bui Thi Thien Thanh, Vo Ngoc Thao Nguyen, and Vo Van Song Toan* 92 57. Determination of the effects of acid citric supplementation and nitrogen-phosphorus deficiency on the lipid accumulation of Nannochloropsis sp. Nguyen Dan Van* and Nguyen Huu Hiep 93 58. Isolation and identification of bacillus subtilis from mud-dreg and waste water of beer production Nguyen Minh Thuy and Tran Nhan Dung* 94 59. Production and optimization of guava wine Pham Hong Quang* and Ha Thien Huong 95 60. Yield and antibacterial activity of Ganoderma Lucidum on bagasses and sawdust substrates Bui Thi My Huong, Tran Non Nuoc*, and Tran Nhan Dung 96 61. Design specific primers for the detection of the entomopathogenic fungus METARHIZIUM NISOPLIAE VAR. ACRIDUM by PCR technique Truong Khanh Anh Co and Tran Thi Xuan Mai* 97 62. Isolation of high yield phosphate solubilizing bacteria associated with sugarcane Nguyen Thi Hong Phuong and Nguyen Huu Hiep* 98 63. Effect of thyroid hormones on porcine oocyte maturation in vitro Walker E.*, Vassiliev I., Mclfatrick S., and Nottle M.B 99 Biotechnology Research and Development Institute, Can Tho University, Can Tho, Vietnam Biotechnology Research and Development Institute, Can Tho University, Can Tho, Vietnam Biotechnology Research and Development Institute, Can Tho University, Can Tho, Vietnam Biotechnology Research and Development Institute, Can Tho University, Can Tho, Vietnam Biotechnology Research and Development Institute, Can Tho University, Can Tho, Vietnam Biotechnology Research and Development Institute, Can Tho University, Can Tho, Vietnam Biotechnology Research and Development Institute, Can Tho University, Can Tho, Vietnam Reproductive Biotechnology Group, School of Pediatrics and Reproductive Health & Centre for Stem Cell Research, Robinson Institute, University of Adelaide, Adelaide, South Australia, Australia