i
th
PROCEEDING OF THE 10 ANNUAL CONFERENCE OF THE
ASIAN REPRODUCTIVE BIOTECHNOLOGY SOCIETY
Sea-links Beach Resort Hotel, Mui Ne, Vietnam
August 19 - 25, 2013
“Innovation in Biotechnology for Health, Medicine and Agriculture”
Edited on behalf of the Society by Program Chair
Nguyen Van Thuan, Ph.D.
International University, Vietnam National University HCMC, Vietnam
Tan Tao University, Ho Chi Minh City, Vietnam
2013 ARBS SCIENTIFIC STEERING COMMITTEES
ARBS SCIENTIFIC STEERING COMMITTEE
2013 ORGANIZING COMMITTEE
Dr. A.W.S. Chan (China)
Dr. Atsuo Ogura (Japan)
Dr. Bui Hong Thuy (Vietnam)
Dr. Changxin Wu (China)
Dr. Chong Li (China)
Dr. Eimei Sato (Japan)
Dr. Graham Jenkin (Australia)
Dr. Hoon Taek Lee (South Korea)
Dr. Hung-Chih Kuo (Taiwan)
Dr. Il-keun Kong (Korea)
Dr. Jin-Hoi Kim (South Korea)
Dr. Ke-Huan Lu (China)
Dr. Mark Nottle (Australian)
Dr. Mashashi Miyake (Japan)
Dr. Mongkol Techakumphu (Thailand)
Dr. Nam-Hyung Kim (South Korea)
Dr. Naojiro Minami (Japan)
Dr. Nisar A.Wani (UAE)
Dr. Noboru Manabe (Japan)
Dr. Nguyen Van Thuan (Vietnam)
Dr. Qing-Yuan Sun (China)
Dr. Ramli Abdullah (Malaysia)
Dr. Rangsun Parnpai (Thailand)
Dr. Sandeep Goel (India)
Dr. Satoshi Kishigami (Japan)
Dr. SC Ng (Singapore)
Dr. Shaorong Gao (China)
Dr. Shien Zhu (China)
Dr. Takashi Miyano (Japan)
Dr. Takashi Nagai (Japan)
Dr. Teoan Kim (South Korea)
Dr. Teruhiko Wakayama (Japan)
Dr. Xiuchun Cindy Tian (China)
Dr. Xuan Nguyen Bui (Vietnam)
H.E. Minister Nguyen Quan, Chairman
Ministry of Science and Technology of Vietnam
Prof. Phan Thanh Binh, Co-Chairman
President
Vietnam National University, HCMC, Vietnam
Prof. Ho Thanh Phong, Co-Chairman
Rector
International University, VNU-HCMC, Vietnam
Prof. Vo Tong Xuan, Co-Chairman
Rector
Tan Tao University, Long An, Vietnam
Prof. Nguyen Van Thuan, Advisor
International University, VNU-HCMC, Vietnam
Tan Tao University, Long An, Vietnam
Secretary-general:
Ms. Vo Ngoc Quynh Nhu
International University, VNU-HCMC, Vietnam
Ms. Nguyen Thi Mai Tram
International University, VNU-HCMC, Vietnam
Secretary:
Dr. Bui Chi Bao
Dr. Nguyen Thi Thu Hoai
Mr. Nguyen Hong Long
Mr. Nguyen Thien Quang
Mr. Phan Cong Hoang
Mr. Ton Ngoc Bao
Ms. Tran Thi Quynh Dao
Ms. Tran Thi Nhu Hao
Ms. Le Tran Hong Ngoc
Ms. Truong Thi Bich Lieu
ARBS ORGANIZING OFFICE
President: Dr. Nguyen Van Thuan
Website Administrator: Mr. Toan Dang Phong
Secretary: Ms. Vo Ngoc Quynh Nhu
Email: arbs@arbsociety.org
Co-hosted by:
Ministry of Science and Technology, VIETNAM
International University, Vietnam National University HCMC, VIETNAM
Tan Tao University, Long An, VIETNAM
ii
The Conference Steering Committee of The Asian Reproductive Biotechnology would
like to Acknowledge and Thank the Following Organization and Companies for Their
Support and Sponsor to this Conference:
MINISTRY OF SCIENCE AND TECHNOLOGY
OF VIETNAM, VIETNAM
http://www.most.gov.vn/Desktop.aspx/Home-EN/
INTERNATIONAL UNIVERSITY, VIETNAM
NATIONAL UNIVERSITY OF HO CHI MINH CITY, VIETNAM
http://www.hcmiu.edu.vn/vn/
TAN TAO UNIVERSITY, LONG AN, VIETNAM
http://ttu.edu.vn/index.php?language=vn
6
PRIME TECH K.K
http://www.primetech-jp.com/
HAMILTON THORNE BIOSCIENCES K.K
http://www.hamiltonthorne.com/
VK SCIENTIFIC EQUIPMENT CO., LTD
http://vietkhoaco.blogspot.com/
SAIGON INSTRUMENTATION JOINT STOCK COMPANY
http://www.sisc.com.vn/
BCE VIETNAM CO., LTD
http://www.bcevietnam.com.vn/
Contents
10th Annual Conference of Asian Reproductive Biotechnology Society 2013
iii
ASIAN REPRODUCTIVE BIOTECHNOLOGY SOCIETY
The Tenth Annual Conference ARBS 2013
Sea-links Beach Resort Hotel, Mui Ne, Vietnam
August 19 - 25, 2013
Conference Program
Monday 19th August, 2013
14:00 PM – 16:00 PM
Registration & Local organizing committee meeting
16:00 PM – 18:00 PM
ARBS Executive Committee Meeting
18:00 PM – 20:00 PM
Welcome Party for ARBS Executive Committee and previous Local
Organizing Committee
Tuesday 20th August, 2013
7:00 AM – 8:30 AM
Registration
8:30 AM – 10:30 AM
-
Opening remarks and welcome by:
H.E. Minister Nguyen Quan
Ministry of Science and Technology, Vietnam
Professor Phan Thanh Binh
President
Vietnam National University, HCMC, Vietnam
Professor Ho Thanh Phong
Rector
International University, VNU-HCMC, Vietnam
Professor Vo Tong Xuan
Rector
Tan Tao University, Long An, Vietnam
-
Overview of the 10 Years ARBS establishment by ARBS
Chairman
-
Take a Photograph for all the participants
Session I
Session Chairs: Dr. Atsuo Ogura, Dr. Ji Wu
10:30 AM – 11:30 AM
Cells into tissues: a cadherin story
Dr. Masatoshi Takeichi
Contents
10th Annual Conference of Asian Reproductive Biotechnology Society 2013
iv
RIKEN Center for Developmental Biology, Kobe, Japan
11:30 AM – 12:10 PM
Genomic reprogramming errors do not accumulate with serial
recloning in the mouse
Dr. Teruhiko Wakayama
University of Yamanashi, Japan
12:10 PM – 13:30 PM
Lunch Break
Session II
Session Chairs: Dr. Teruhiko Wakayama, Dr. Qing-Yuan Sun
13:30 PM – 14:00 PM
In vivo and in vitro differentiation of postnatal female rat germline
stem cells into oocytes
Dr. Ji Wu
Shanghai Jiaotong University, China
14:00 PM – 14:30 PM
Research on goat cloning and stem cell for possible application in
agriculture and human medicine in Malaysia
Dr. Ramli Abdullah
Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603
Kuala Lumpur, Malaysia
14:30 PM – 15:00 PM
Mitochondrial during human oocyte maturation - aiming at
mitochondrial transfer
Dr. Yoshiharu Morimoto
CEO, IVF Japan
15:00 PM – 15:10 PM
Break
Session III
Session Chairs: Dr. Haruhiko Koseki, Dr. Yoshiharu Morimoto
15:10 PM – 15:40 PM
Microfilaments and microtubules in oocytes maturation: current
frontiers and beyond
Dr. Nam-Hyung Kim
Chungbuk National University, Republic of Korea
15:40 PM – 16:10 PM
The role of oocyte nucleolus precursor bodies (NPBs)
Dr. Helena Fulka
Institute of Animal Science, Pratelstvi 815, Prague, Czech Republic
16:10 PM – 16:40 PM
Identification, proliferation, and reprogramming of putative stem
cell in the adult pig ovary
Dr. Hong-Thuy Bui
International University, Vietnam National University HCMC, Vietnam
Tan Tao University, Long An, Vietnam
16:40 PM – 17:10 PM
Survivin is essential for fertile egg production and female fertility
in mice
Contents
10th Annual Conference of Asian Reproductive Biotechnology Society 2013
v
Dr. Qing-Yuan Sun
Chinese Academy of Sciences, Beijing 100101, China
19:00 PM – 22:00 PM
Welcome party
Wednesday 21st August, 2013
Session IV
Session Chairs: Dr. Masatoshi Takeichi, Dr. Mark B. Nottle
8:00 AM – 8:30 AM
Status of transcription regulation in mammalian oocytes and
embryos
Dr. Dong Il Jin
Chungnam National University, Daejeon 305-764, Korea
8:30 AM – 9:00 AM
The role of enhancer of polycomb during spermiogenesis
Dr. Haruhiko Koseki
RIKEN Center for Integrative medical Science
9:00 AM – 9:30 AM
Epigenetic reprogramming of somatic cells by nuclear transfer,
chemical and viral inductions
Dr. Xiuchun (Cindy)Tian
Center for Regenerative Biology, University of Connecticut, United States
9:30 AM – 10:00 AM
Recent developments in assisted reproduction in camels
Dr. Nisar Wani
Camel Reproductive Center Dubai, UAE
10:00 AM – 10:10 AM
Break
Session V
Session Chairs: Dr. Nguyen Van Thuan, Dr. Xiuchun Cindy Tian
10:10 AM – 10:40 AM
Influence of acupuncture stimulation on pregancy rates for
women undergoing embryo transfer at infertility department,
Hung Vuong hospital
Dr. Ly Thai Loc
Hung Vuong Hospital, Ho Chi Minh City, Vietnam
10:40 AM – 11:05 AM
Results of modified protocols in in-vitro maturation (IVM) of
oocytes
Dr. Ho Manh Tuong
Research Center for Genetics and Reproductive Health (CGRH), School of
Medicine, Vietnam
11:05 AM – 11:30 AM
Growth retardation in human blastocysts decreases their
implantation potential possibly due to an increase of the incidence
Contents
10th Annual Conference of Asian Reproductive Biotechnology Society 2013
vi
of abnormal spindles
Dr. Shu Hashimoto
IVF Namba Clinic, Nishiku, Osaka, Japan
11:30 AM – 11:55 AM
Isolation of porcine embryonic stem cells from heterozygous
parthenogenetic embryos
Dr. Mark B. Nottle
University of Adelaide, Adelaide, Australia
11:55 AM – 13:00 PM
Lunch Break
Session VI
Session Chairs: Dr. Satoshi Kishigami, Dr. Mukesh Gupta
13:00 PM – 13:30 PM
Generation of mammalian haploid embryonic stem cells
Dr. Jinsong Li
Shanghai Institutes for Biological Sciences Chinese Academy of Sciences, China
13:30 PM – 14:00 PM
Cord lining stem cells – a novel stem cell platform for tissue
regeneration with promising commercial potential in the sphere of
regenerative medicine
Dr. Thang Toan Phan
Department of Surgery, Yong Loo Lin School of Medicine, National University of
Singapore Cell Research Corp, Singapore
Session VII
Poster session (with break)
14:05 PM – 16:30 PM
Poster session
16:30 PM – 18:30 PM
Demonstration of ICSI, SCNT, Embryonic manipulation
Thursday 22nd August, 2013
Session VIII
Session Chairs: Dr. Teoan Kim, Dr. Phan Toan Thang
8:00 AM – 8:30 AM
Inter-species and inter-generic somatic cell nuclear transfer:
challenging for conservation endangered species
Dr. Rangsun Parnpai
Suranaree University of Technology, Thailand
8:30 AM – 9:00 AM
Reprogramming events in somatic cells induced by extract from
germinal vesicle (GV) stage pig oocytes
Dr. Nguyen Van Thuan
Department of Animal Biotechnology, Konkuk University, Korea
9:00 AM – 9:30 AM
Stem cells research and application in Vietnam, results and
Contents
vii
10th Annual Conference of Asian Reproductive Biotechnology Society 2013
perspectives
Dr. Bui Xuan Nguyen
Institute of Biotechnology, Vietnam
9:30 AM – 9:40 AM
Break
Session IX
Session Chairs: Dr. Nam-Hyung Kim, Dr. Bui Xuan Nguyen
9:40 AM – 10:05 AM
Sperm: not just a carrier of father’s DNA
Dr. Mukesh Gupta
Department of Animal Biotechnology, Konkuk University, Korea
10:05 AM – 10:30 AM
Porcine blastocyst vitrification after artificial shrinkage of the
blastocoele
Dr. Marlon B. Ocampo
Reproductive Biotechnology Unit, Philippines
10:30 AM – 10:55 AM
Recent advances in
asymmetric division
Dr. Shao-Chen Sun
the
molecular
mechanism
of
oocyte
College of Animal Science and Technology, China
Session X
Session Chairs: Dr. Ke-Huan Lu, Dr. Rangsun Parnpai
10:55 AM – 11:20 AM
Hypoosmotic swelling test to assess the functional membrane
integrity of water buffalo spermatozoa
Dr. Danilda –Hufana Duran
Reproductive Biotechnology Laboratory, Philippines
11:20 AM – 11:45 AM
Reproductive effects of two neonicotinoid insecticides on mouse
sperm function and early embryonic development in vitro
Dr. Li Yan
Reproductive Medical Center, China
11:45 AM – 12:10 PM
Follicular dynamics during ovum pick-up at different intervals in
water buffalo
Dr. Peregrino G. Duran
Reproductive Biotechnology Laboratory, Philippines
12:10 PM – 13:30 PM
Session XI
13:30 PM – 13:50 PM
Lunch Break
Session Chairs: Dr. Jinsong Li, Dr. Ramli B. Abdullah
Determination of hybrid individuals between domestic female cow
(bos taurus) and wild male gaur (bos gaurus) in national park of
Phuoc Binh - adjointive forests of Lam Dong-Ninh Thuan-Khanh
Hoa
Contents
10th Annual Conference of Asian Reproductive Biotechnology Society 2013
viii
Dr. Le Xuan Tham
Department of Science and Technology, Vietnam
13:50 PM – 14:10 PM
In vitro tert mRNA expression in relation to chromosomal stability,
senescence and telomere length and SCNT cloned embryo
production in bubalus bubalis
Dr. Neelam Gupta
Transgenic Research Laboratory, India
14:10 PM – 14:30 PM
Effect of space environment on mammalian reproduction (space
pup)
Dr. Sayaka Wakayama
Center for Developmental Biology (CDB), RIKEN, Japan
Session XII
Session Chairs: Dr. Dong Il Jin, Dr. Chong Li
14:30 PM – 14:50 PM
The roles of tet genes in mouse early embryo development and
pig induced pluripotent stem cells
Dr. Ziyi Li
Jilin University, China
14:50 PM – 15:10 PM
Vitrification of in-vitro matured goat (capra hircus) oocytes using
cryotop method optimized with cytochalasin-b
Dr. Eufrocina P. Atabay
Reproductive Biotechnology Unit, Philippines
Session XIII
Session Chairs: Dr. Ho Manh Tuong, Dr. Nisar Ahmad Wani
15:10 PM – 15:30 PM
Establish MESC lines using long-term preserved mouse sperm at
refrigerator temperature
Dr. Chong Li
Advanced Institute of Translational Medicine, Tongji University, China
15:30 PM – 15:50 PM
Optimized sequential culture system for the production of bovine
(bos taurus) embryos in-vitro
Dr. Edwin C. Atabay
Philippine Carabao Center at CLSU, Science City of Munoz, Philippines
15:50 PM – 16:10 PM
Cryopreservation of immature and in vitro matured goat oocytes
by the cryoloop vitrification method
Dr. Guo Bo Quan
Yunnan Animal Science and Veterinary Institute, Jindian, Yunnan Province, China
Session XIV
Closing Ceremony
Session Chairs: Dr. Ho Thanh Phong, Dr. Vo Tong Xuan &
Dr. Nguyen Van Thuan
16:10 PM – 16:30 PM
Award for Poster Presentation
Contents
10th Annual Conference of Asian Reproductive Biotechnology Society 2013
16:30 PM – 16:40 PM
Introduce the hosted country for the 11th ARBS meeting
16:40 PM – 17:00 PM
Closing Ceremony by Local Organizing Committee
18:30 PM – 22:00 PM
Farewell Party
ix
Friday 23 – Saturday 24 August, 2013: Post-conference Scientific Trip
Contents
10th Annual Conference of Asian Reproductive Biotechnology Society 2013
x
PROCEEDINGS OF THE 10TH ANNUAL CONFERENCE OF THE ASIAN REPRODUCTIVE
BIOTECHNOLOGY SOCIETY
Sea-links Beach Resort, Mui Ne, Vietnam, August 19 - 25, 2013
PLATFORM SESSION
Cells into tissues: a cadherin story
Masatoshi Takeichi
1
Genomic reprogramming errors do not accumulate with serial recloning in the mouse
Teruhiko Wakayama
2
In vivo and in vitro differentiation of postnatal female rat germline stem cells into oocytes
Ji Wu
3
Research on goat cloning and stem cell for possible application in agriculture
and human medicine in Malaysia
Ramli Abdullah
4
Mitochondrial during human oocyte maturation - aiming at mitochondrial transfer
Yoshiharu Morimoto
5
Microfilaments and microtubules in oocytes maturation: current frontiers and beyond
Nam-Hyung Kim
6
RIKEN Center for Developmental Biology, Kobe, Japan
University of Yamanashi, Japan
Shanghai Jiaotong University, China
Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia
CEO, IVF Japan
Chungbuk National University, Republic of Korea
The role of oocyte nucleolus precursor bodies (NPBs)
Helena Fulka
7
Institute of Animal Science, Pratelstvi 815, Prague, Czech Republic
Identification, proliferation, and reprogramming of putative stem cell in the adult pig ovary
Hong-Thuy Bui
8
Survivin is essential for fertile egg production and female fertility in mice
Qing-Yuan Sun
9
Status of transcription regulation in mammalian oocytes and embryos
Dong Il Jin
10
The role of enhancer of polycomb during spermiogenesis
Haruhiko Koseki
11
Epigenetic reprogramming of somatic cells by nuclear transfer, chemical and viral inductions
Xiuchun (Cindy)Tian
12
International University, Vietnam
Tan Tao University, Long An, Vietnam
Chinese Academy of Sciences, Beijing 100101, China
Chungnam National University, Daejeon 305-764, Korea
RIKEN Center for Integrative medical Science
Center for Regenerative Biology, University of Connecticut, United States
Contents
10th Annual Conference of Asian Reproductive Biotechnology Society 2013
xi
Recent developments in assisted reproduction in camels
Nisar Wani
13
Influence of acupuncture stimulation on pregancy rates for women undergoing
embryo transfer at infertility department, Hung Vuong hospital
Ly Thai Loc
14
Camel Reproductive Center Dubai, UAE
Hung Vuong Hospital, Ho Chi Minh City, Vietnam
Results of modified protocols in in-vitro maturation (IVM) of oocytes
Ho Manh Tuong
15
Growth retardation in human blastocysts decreases their implantation potential possibly
due to an increase of the incidence of abnormal spindles
Shu Hashimoto
16
Isolation of porcine embryonic stem cells from heterozygous parthenogenetic embryos
Mark B. Nottle
17
Generation of mammalian haploid embryonic stem cells
Jinsong Li
18
Cord lining stem cells – a novel stem cell platform for tissue regeneration with promising
commercial potential in the sphere of regenerative medicine
Thang Toan Phan
19
Inter-species and inter-generic somatic cell nuclear transfer: challenging for conservation
endangered species
Rangsun Parnpai
20
Induced pluripotent stem cell by GV oocytes extract: gvIPS Cells
Nguyen Van Thuan
21
Stem cells research and application in Vietnam, results and perspectives
Bui Xuan Nguyen
22
Sperm: not just a carrier of father’s DNA
Mukesh Gupta
23
Porcine blastocyst vitrification after artificial shrinkage of the blastocoele
Marlon B. Ocampo
24
Recent advances in the molecular mechanism of oocyte asymmetric division
Shao-Chen Sun
25
Research Center for Genetics and Reproductive Health (CGRH), School of Medicine, Vietnam
IVF Namba Clinic, Nishiku, Osaka, Japan
University of Adelaide, Adelaide, Australia
Shanghai Institutes for Biological Sciences Chinese Academy of Sciences, China
Department of Surgery, Yong Loo Lin School of Medicine, National University of Singapore
Cell Research Corp, Singapore
Suranaree University of Technology, Thailand
International University, Vietnam National University HCMC, Vietnam
TanTao University, Long An, Vietnam
Institute of Biotechnology, Ha Noi, Vietnam
Department of Animal Biotechnology, Konkuk University, Korea
Reproductive Biotechnology Unit, Philippines
College of Animal Science and Technology, China
Contents
10th Annual Conference of Asian Reproductive Biotechnology Society 2013
xii
Hypoosmotic swelling test to assess the functional membrane integrity of
water buffalo spermatozoa
Danilda –Hufana Duran
26
Reproductive effects of two neonicotinoid insecticides on mouse sperm function and
early embryonic development in vitro
Li Yan
27
Follicular dynamics during ovum pick-up at different intervals in water buffalo
Peregrino G. Duran
28
Determination of hybrid individuals between domestic female cow (bos taurus) and
wild male gaur (bos gaurus) in national park of Phuoc Binh - adjointive forests of
Lam Dong-Ninh Thuan-Khanh Hoa
Le Xuan Tham
29
In vitro tert mRNA expression in relation to chromosomal stability, senescence and
telomere length and SCNT cloned embryo production in bubalus bubalis
Neelam Gupta
30
Effect of space environment on mammalian reproduction (space pup)
Sayaka Wakayama
31
The roles of tet genes in mouse early embryo development and pig induced pluripotent
stem cells
Ziyi Li
32
Vitrification of in-vitro matured goat (capra hircus) oocytes using cryotop method
optimized with cytochalasin-b
Eufrocina P. Atabay
33
Establish MESC lines using long-term preserved mouse sperm at refrigerator temperature
Chong Li
34
Optimized sequential culture system for the production of bovine (bos taurus) embryos in-vitro
Edwin C. Atabay
35
Cryopreservation of immature and in vitro matured goat oocytes by the cryoloop
vitrification method
Guo Bo Quan
36
Reproductive Biotechnology Laboratory, Philippines
Reproductive Medical Center, China
Reproductive Biotechnology Laboratory, Philippines
Department of Science and Technology, Vietnam
Transgenic Research Laboratory, India
Center for Developmental Biology (CDB), RIKEN, Japan
Jilin University, China
Reproductive Biotechnology Unit, Philippines
Advanced Institute of Translational Medicine, Tongji University, China
Philippine Carabao Center at CLSU, Science City of Munoz, Philippines
Yunnan Animal Science and Veterinary Institute, Jindian, Yunnan Province, China
Contents
10th Annual Conference of Asian Reproductive Biotechnology Society 2013
xiii
POSTER SESSION
1.
SGO1 maintains bovine meiotic centromeric cohesions of sister chromatids and
directly affects embryo development
Feng-xia Yin and Guang-peng Li*
37
The Key Laboratory of National Education Ministry for Mammalian Reproductive Biology and Biotechnology, Inner
Mongolia University, Inner Mongolia, Hohhot, China
2.
Development patterns of water buffalo (bubalus bubalis) IVEP embryos at various age
38
and stages
Prudencio B. Pedro*, Marlon B. Ocampo, Eufrocina P. Atabay, Flocerfida P. Aquino,
Lerma C. Ocampo, Excel Rio Maylem, and Edwin C. Atabay
Reproductive Biotechnology Laboratory, Office of the Executive Director, Philippine Carabao Center, National
Headquarters and Gene Pool, Science city of Munoz 3120, Nueva Ecija, Philippines
3.
Toxicity and protective efficiency of cryoprotectants to catfish (pangasidae hypophthamus)
embryos stored at low temperature
Nguyen Thi Hong1*, Nguyen Thi Nhung1, Nguyen Thi Uoc1, Nguyen Thi Hiep1,
Nguyen Viet Linh1, Nguyen Viet Hanh1, Pham Van Khanh2, Ha Thi Ngoc Nga2,
Tran Van Thuong3, and Bui Xuan Nguyen1*
39
Effect of immunomodulators on reproductive performance of sahiwal cows
Anjali Aggarwal* and Gulab Chandra
40
Can glucose and oxygen levels affect the development and sex ratio in mouse
embryo culture?
Mai Tram Nguyen1,2*, Ji Yeon Cailyn Kim2, and Zhen Xiang2
41
1)
Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, Vietnam; 2)Research Institute
for Aquaculture No.2, Ho Chi Minh City, Vietnam; 3)Research Institute for Aquaculture No.1, Vietnam
4.
Dairy Cattle Physiology Division, National Dairy Research Institute, Karnal-132001 (Haryana), India
5.
1)
Department of Biotechnology, School of Biotechnology, International University, Vietnam National University, Ho
Chi Minh City, Vietnam; 2)Education Program in Reproduction and Development (EPRD), Department of Obstetrics
and Gynaecology, Monash University, Clayton, Australia
6.
The effectiveness of hyaluronic acid versus polyvinylpyrrolidone in intracytoplasmic
42
sperm injection
Kha TH Le1,2*, Quynh N Nguyen1,2, Duy H Nguyen1,2, Khang A Nguyen2, Mai T Nguyen1,
Bich N Nguyen1,2, Lian S Liow2, and Vinh Q Dang1,2
1)
IVFVH , Van Hanh General Hospital, Vietnam; 2)Research Center for Genetics and Reproductive Health (CGRH),
School of Medicine, Vietnam National University - Ho Chi Minh City, Vietnam
7.
Live-cell imaging analysis of mouse oocytes injected with freeze-dried spermatozoa
Eiji Mizutani*, Sayaka Wakayama, Kana Kishida, and Teruhiko Wakayama
43
Effects of taxol in cryopreservation of mature bovine oocytes by vitrification
Nguyen Thi Thuong Huyen1, 2*, Le Thanh Long3, Nguyen Quoc Dat4,
Hoang Nghia Son3, and Phan Kim Ngoc1
44
Faculty of Life and Environmental Sciences, University of Yamanashi, Kofu, Japan
8.
1)
University of Science, Vietnam National University, Ho Chi Minh City, Vietnam; 2)Department of Animal
Physiology, Faculty of Biology, University of Pedagogy, Ho Chi Minh City, Vietnam; 3)Institute of Tropical
Biology, VAST, Vietnam; 4)National Institute of Animal Husbandry, Vietnam
Contents
9.
10th Annual Conference of Asian Reproductive Biotechnology Society 2013
xiv
Measurement reactive oxygen species (ROS) levels in neat semen of 600 infertile couples
45
Huynh Thi Hong Vinh1*, Nguyen Ngoc Yen Nhi1, Tran Tu Cam1, Nguyen Thi Lien Thi1,
Nguyen Thi Mai1, and Ho Manh Tuong1,2
1)
Ngoc Lan Clinic, Ho Chi Minh City, Vietnam; 2)Research Center for Genetics and Reproductive Health (CGRH),
School of Medicine, Vietnam National University - Ho Chi Minh City, Vietnam
10. Preimplantation genetic diagnosis on day 3 human embryos
Lan TT Nguyen1,2*, Hoang VM Bui3, Binh TT Truong1, Thuc N Vo2, Vinh Q Dang2,
Tuong M Ho1,2, and Kiet D Truong3
46
1)
IVFAS, An Sinh General Hospital, Ho Chi Minh City, Vietnam; 2)Research Center for Genetics and Reproductive
Health (CGRH), School of Medicine, Vietnam National University - Ho Chi Minh City, Vietnam; 3)University of
Medicine and Pharmacy, Ho Chi Minh City, Vietnam
11. Development of non-surgical embryo transfer technique for production of transgenic cow
47
Sang Jun Uhm1*, Jung Seok Yang1, Young-Tae Heo2, Yong-Nan Xu2, Bon-Chul Koo3,
Nam-Hyung Kim2, and Dae Hwan Ko1
1)
Department of Animal Science and Biotechnology, Sangji Youngseo College, Wonju, South Korea; 2)Department
of Animal Science, Chungbuk National University, Cheongju, South Korea; 3)Department of Physiology, Catholic
University of Daegu School of Medicine, Daegu, South Korea.
12. Effect of application modified IMSI in assisted reproduction
Bao G Huynh1,2*, Anh H Le1,2, Xuan T Pham1, Nghia T Luong2, Tuan A Lam1,2,
and Tuong M Ho1,2
48
1)
IVF An Sinh, An Sinh General Hospital, Ho Chi Minh City, Vietnam; 2)Research Center for Genetics and
Reproductive Health (CGRH), School of Medicine, Vietnam National University - Ho Chi Minh City, Vietnam
13. Rho-GTPase effector rock regulates actin-mediated cytokinesis in mouse oocyte
49
Xing Duan1, Zhen-Bo Wang3, Xiang-Shun Cui2, Nam-Hyung Kim2, Qing-Yuan Sun3,
and Shao-Chen Sun1*
1)
Nanjing Agricultural University, Nanjing, China; 2)Chungbuk National University, Cheongju, Republic of Korea;
University of Chinese Academy of Sciences, Beijing, China
3)
14. Artifical oocyte activation by using calcium ionophore a23187 in severe
teratozoospermia patients
Lan TT Nguyen1* and Tuong M Ho1,2
50
1)
IVF An Sinh, An Sinh General Hospital, Ho Chi Minh City, Vietnam; 2)Research Center for Genetics and
Reproductive Health (CGRH), School of Medicine, Vietnam National University - Ho Chi Minh City, Vietnam
15. Vitrification of transgenic bovine embryos by PNC container
51
Dae Hwan Ko*1, Jung Seok Yang1, Joon Gyo Lim2, Young-Tae Heo2, Yong-Nan Xu2,
Bon-Chul Koo3, Nam-Hyung Kim2, and Sang Jun Uhm1
1)
Department of Animal Science and Biotechnology, Sangji Youngseo College, Wonju, South Korea; 2)Department
of Animal Science, Chungbuk National University, Cheongju, South Korea; 3)Department of Physiology, Catholic
University of Daegu School of Medicine, Daegu, South Korea.
16. Comparison of 5% and ambient oxygen during days 1-3 of in vitro culture of
human embryos
An Vo*
52
Hanh Phuc Hospital, Ho Chi Minh City, Vietnam
17. Ethanol sclerotherapy after ultrasound-guided aspiration can prolong the time to recurrence 53
of endometrial cyst
Yoshiharu Morimoto, Shu Hashimoto, Yoshiharu Nakaoka, Tomoko Inoue, Takao
Himeno, Yoko Ohnishi, Rie Nakahira, and Keijiro Ito*
Medical Department, IVF Namba Clinic, Nishiku, Osaka, Japan
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10th Annual Conference of Asian Reproductive Biotechnology Society 2013
xv
18. Mycotoxins containing diet decreases oocyte quality in mouse
Cheng-Cheng Zhu1, Ziang-Shun Cui2, Nam-Hyung Kim2, and Shao-Chen Sun1*
54
1)
College of Animal Science and Technology, Nanjing Agricultural University, Jiangsu, Nanjing, China;
Department of Animal Science, Chungbuk National University, Chungbuk, Cheongju, Republic of Korea
2)
19. The correlation between the morphological characters of the oocyte and the ICSI outcomes
Dung TP Nguyen1*, Lan TT Nguyen1,2, Xuan T Pham2, and Tuong M Ho1,2
55
1)
IVF An Sinh, An Sinh General Hospital, Ho Chi Minh City, Vietnam
Research Center for Genetics and Reproductive Health (CGRH), School of Medicine, Vietnam
National University - Ho Chi Minh City, Vietnam
2)
20. Viability of extended goat semen stored at refrigerated condition
Flocerfida P. Aquino1*, Krystel Grace B. Vergara2, Lerma C. Ocampo1,
and Eufrocina P. Atabay1
56
1)
Reproductive Biotechnology Unit, Philippine Carabao Center, Science City of Munoz, Nueva Ecija, Phillipines,
Central Luzon State University, Science City of Munoz, Nueva Ecija, Philippines
2)
21. The maturation rate and ICSI outcomes of different morphological classifications of
germinal vesicle (gv) oocytes
Lan TT Nguyen1,2*, Xuan T Pham1, and Lan TN Vuong1,3
57
22. Effect of varying sperm concentration on the post-thaw liveability of frozen goat semen
Lerma C. Ocampo*1, Flocerfida P. Aquino1, Edeneil Jerome P. Valete2,
Marlon B. Ocampo1,2, and Eufrocina P. Atabay1
58
1)
IVF An Sinh, An Sinh General Hospital, Ho Chi Minh City, Vietnam; 2)Research Center for Genetics and
Reproductive Health (CGRH), School of Medicine, Vietnam National University - Ho Chi Minh City, Vietnam;
3)
University of Medicine and Pharmacy, Ho Chi Minh City, Vietnam
1)
Philippine Carabao Center–Reproductive Biotechnology Unit at National Headquarters and Genepool, Science
City of Munoz, Nueva Ecija, Philippines; 2) Central Luzon State University- Institute of Graduate Studies, Science
City of Munoz, Nueva Ecija, Philippines
23. Effect of medium with GM – CSF on repeated implantation failure in assisted reproduction
Bao G Huynh1,2*, Lan TT Nguyen1,2, Tram TB Le2, and Vinh Q Dang1,2
59
24. Ensuring the quality of bovine embryos produced in-vitro through the inner cell mass
and trophectoderm ratio
ER. S. Maylem*, E. P. Atabay, L. C. Ocampo, M. B. Ocampo, P. B. Pedro, and
F. P. Aquino
60
1)
IVF An Sinh, An Sinh General Hospital, Ho Chi Minh City, Vietnam; 2)Research Center for Genetics and
Reproductive Health (CGRH), School of Medicine, Vietnam National University - Ho Chi Minh City, Vietnam
Reproductive Biotechnology Unit, Philippine Carabao Center, National Headquarters, Science City of Muñoz,
Nueva Ecija, Philippines
25. Critical role of mitochondrial activity in the porcine oocyte in vitro maturation
Lee Seul Ki, Zhao Minghui, Kwon Jung-Woo, Li Yinghua, Lin Zili, Jin Yongxun,
Kim Nam-Hyung, and Cui Xiangshun*
61
Department of Animal Sciences, Chungbuk National University, South Korea
26. Effect of extra-nucleolus injection on development of icsi and SCNT embryos
Hirohisa Kyogoku1,2*, Teruhiko Wakayama2,3, and Takashi Miyano1
1)
Graduate School ofAgricultural Science, Kobe University, Kobe 657-8501, Japan; 2)Laboratory for Genomic
Reprogramming, Center for Developmental Biology, RIKEN, Kobe 650-0047, Japan; 3)Faculty of Life and
Environmental Sciences, University of Yamanashi, Kofu 400-8501, Japan
62
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10th Annual Conference of Asian Reproductive Biotechnology Society 2013
xvi
27. Greatwall kinase is required for meiotic maturation in porcine oocytes
Ying-Hua Li1, Hyoen Kang2, Yong-Nan Xu1, Young- Tae Heo1, Xiang-Shun Cui1,
Nam-Hyung Kim1*, and Jeong Su Oh2*.
63
28. Similar but distinct in vitro aging phenotypes of mouse SCNT oocytes
Satoshi Kishigami*, Ah Reum Lee, Thanh Ha Le, and Yoshihiko Hosoi
64
1)
Deparment of Animal Science, Chungbuk National University, Chungbuk, Cheongju, Republic of Korea, 2)Genetic
Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University, Gyeonggi-do, Suwon, Korea.
Department of Biology-Oriented Science and Technology, Kinki University, Wakayama, Kinokawa, Japan
29. Production of transgenic cynomolgus monkey embryos using interspecies somatic cell
nuclear transfer
Yazaki Satoko1, Oishi Takatsugu1, Inoue Kimiko2, Ogura Atsuo2, and
Sankai Tadashi3*
65
1)
Deparment of Advanced Technology Development team, PRIME TECH LTD, Ibaraki-ken, Tshuchiura-shi, Japan,
RIKEN BioResource Center, Ibaraki-ken, Tsukuba-shi, Japan; 3)Tsukuba Primate Center for Medical Science,
National Institute of Infectious Diseases, Ibaraki-ken, Tsukuba-shi, Japan
2)
30. Production of cloned mice from a drop of periheral blood
Satoshi Kamimura*, Kimiko Inoue, Narumi Ogonuki, Michiko Hirose, Mami
Oikawa, Masahiro Yo, Osamu Ohara, Hiroyuki Miyoshi, and Atsuo Ogura
66
RIKEN BioResource Center, Ibaraki, Tsukuba, Japan
31. Proliferation rate of ear skin fibroblast cells derived from brakmas cattle of different ages
67
Habsah Bidin1*, Najlaa Zulhemay1, Kwong Phek Jin2, Ahmad Johari1, Ajis Hassan1,
Norjahan Banu Alitheen3, Nadiah Abu3, and Musaddin Kamaruddin4
1)
National Animal Embryo Centre (NAEC), MARDI Kluang Research Station, Locked Bag 525, 86009 Kluang Johore,
Malaysia; 2)Department of Agriculture and Food Science, Faculty of Science, University Tunku Abdul Rahman,
31900 Kampar, Perak, Malaysia; 3)Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia,
43400 Serdang, Selangor, Malaysia; 4)Strategic Livestock Research Centre, MARDI HQ, P.O. Box 12301, 50774;
Kuala Lumpur, Malaysia
32. Generation of induced pluripotent stem cells from goat fibroblasts
Hui Song and Feng Wang*
68
33. Next generation sequencing target to human breast cancer stem cells
Diem-My Vu, Minh Nguyen Anh Ho, and Chi-Bao Bui*
69
34. Stage specific colony formation in vitro cultured spermatogonia derived from porcine,
murine and canine testes
Kyung Hoon Lee, Won Yong Lee, Ran Lee, Hee Chan Kim, and Hyuk Song*
70
35. Goat oocytes from lopu versus abattoir after ear fibroblast cells injection gives better
SCNT performance
Asdiana, A., W.E. Wan Khadijah*, and R.B. Abdullah
71
36. A zinc dependent regulatory mechanism controlling meiotic cell cycle
Zhao Minghui1, Li Yinghua1, Lin Zili1, Oh Jeong-Su2, Kim Nam-Hyung1,
and Cui Xiangshun1*
72
Center of Animal Embryo Engineering and Technology, Nanjing Agricultural University, Nanjing 210095, China
University of Medicine and Pharmacy HCMC, Ho Chi Minh, Vietnam
Department of Animal and Food Bioscience, College of Biomedical and Health Science, Konkuk University,
Chung-ju, Republic of Korea
Animal Biotechnology-Embryo Laboratory (ABEL),
Institute of Biological Sciences, Faculty of Science,
University of Malaya,
50603 Kuala Lumpur, Malaysia
1)
Department of Animal Science Chungbuk National University,Cheongju, 361-763, Korea; 2)Department
of Genetic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University, Suwon,
Contents
10th Annual Conference of Asian Reproductive Biotechnology Society 2013
xvii
Gyeonggi-do, 440-746, Korea
37. Coexpression of hemagglutinin, neuraminidase and M1 matrix proteins in CHO-K1 cell line
Tin C. Pham1*, Thuan D. Lao2, Thu T. N. Le3, and Si M. Do4
73
38. Ameliorative effect of zinc to lead exposed goat kids on lymphocyte proliferation and
superoxide dismutase (SOD) expression
Harjit Kaur1*, Muneendra Kumar2, Neelam Gupta1, Veena Mani3, Neelam J.
Kewalramani3, and B.T. Phondba4
74
39. The expressions of GATA-4 and GFRA-1 in mouse seminiferous epithelium
Guan-yu Che, Yi-xue Sun, Ai-bing Wang, Zi-yi Li, and Xueming Zhang
75
40. First report on the virulence of some Vibrio Cholerae strains isolated in Vietnam recently
Le Thi Thanh Thao1*, Le Tran Hong Ngoc1, Nguyen Thi Thu Hoai1,
and Pham Hung Van2
76
41. Expression and roles of bone morphogenetic protein 15 and growth differentiation
factor 9 in porcine oocytes maturing in vitro
Zi-Li Lin, Xiang-Shun Cui, Nam-Hyung Kim
77
1)
Department of Biotechnology, School of Biotechnology, International University, Vietnam National University,
Ho Chi Minh City, Vietnam; 2)School of Biotechnology, Open University of Ho Chi Minh City, Ho Chi Minh City,
Vietnam; 3)School of Biotechnology, University of Industry of Ho Chi Minh City, Ho Chi Minh City, Vietnam;
4)
Department of Biotechnology, University of Sciences, Vietnam National University, Ho Chi Minh City, Vietnam
1)
Indian Council of Agricultural Research, New Delhi, 110001, India, 2)College of Veterinary Science and
Animal Husbandry, DUVASU, Mathura, 281001, India, 3)National Dairy Research Institute, Karnal, 132001,
India, 4)National Dairy Development Board, Anand, 388001, India
Jilin Province Key Laboratory of Animal Embryo Engineering, and the Center for Animal Embryo Engineering
of Jilin Province, College of Veterinary Medicine, Jilin University, Changchun 130062, China
1)
Department of Biotechnology, School of Biotechnology, International University, Vietnam National University,
Ho Chi Minh City, Vietnam, 2)NamKhoaBiotek company, Ho Chi Minh City, Vietnam
Deparment of Animal Science, Chungbuk National University, Chungbuk, Cheongju, Republic of Korea
42. Roles of actin capping proteins (CAPZ) in mammalian oocyte maturation
Yu-Jin Jo, Suk Namgoong, Wooin Yang, and Nam-Hyung Kim
78
Deparment of Animal Science, Chungbuk National University, Chungbuk, Cheongju,
Republic of Korea
43. Generation of five human lactoferrin transgenic cloned goats using fibroblast cells
and their methylation status of putative IGF2R and H19 imprinted genes
Li Meng, Yongjie Wan, and Feng Wang*
79
44. Examination of the relationship between the mutation RS3741378 of SIPA1 gene and
breast cancer in Vietnamese women
Le Ngoc Yen*, Nguyen Dieu Hoai Chan, Le Tran Hong Ngoc, and Nguyen Thi Hue
80
45. Generation of transgenic chickens producing HGH for oral administration
Bon Chul Koo, Mo Sun Kwon, Sang A Kim, Hae Jin Son, and Teoan Kim
81
46. Localization of the DIPEPTIDYL PEPTIDASE-4 gene in porcine COCS and
pre-implantation embryos
Ji-Hyun Lim, Hak-Jae Chung, Byoung-Chul Yang, Kyoung-Woon Kim,
Sun-Young Back, Won-Gil Lee, Sung-Ku Hong, Woon-Kyoung Chang,
Jin-Ki Park, and Mi-Ryung Park*
82
Center of Animal Embryo Engineering and Technology, Nanjing Agricultural University, Nanjing 210095, China
Department of Biotechnology, School of Biotechnology, International University, Vietnam National University,
Ho Chi Minh City, Vietnam
Department of Physiology, Catholic University of Daegu School of Medicine, Daegu, Korea
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10th Annual Conference of Asian Reproductive Biotechnology Society 2013
xviii
Animal Biotechnology, National Institute of Animal Science, Gyeonggi-do, Suwon, Republic of Korea
47. Effects of serum starvation, roscovitine treatment and contact inhibition on the cell-cycle
synchronization of new-born bama mini-pig transgenic kidney fibroblast cells and the
development of transgenic clone embryo
Linlin Hu, Juan Lou, Xiangxing Zhu, Shouneng Quan, Shengsheng Lu*, and
Kehuan Lu*
83
48. Sex reversal (XY, female) in cloned dog
Hwang Kyu-Chan1, Kim Joung-Joo1, Jung Jong-Hun1, Ko Kyeong-Hee1,
Jeong Yeon-Woo1, Kim Nam-Hyung2, Moon Changjong3, Shin Taeyoung1,
and Hwang Woo-Suk1*
84
49. Comparison of gene expression profiles of cumulus cell of in vivo ovulated oocytes
with in vitro matured oocytes
Su-Jin Cho1,2,Yu-Gon Kim1, Dong-Hoon Kim3, Jae-Gyu Ryoo3, Jin-Ki Park3,
and Il-Keun Kong1,2*
85
50. Establishment of effective knockin-platform using TALENS
Hwang Kyu-Chan1, Jung Jong-Hun1, Ko Kyeong-Hee1, Kim Sun1, Song Seul-Ki1,
Yong Hwan-Yul1, Kim Nam-Hyung2, Shin Taeyoung1, and Hwang Woo-Suk1*
86
51. The freeze-drying of antibody-conjugated gold nanoparticles for
immunodiagnostics of malaria
Nhu Ngoc Quynh Vo1,2*, Masuda N. Khursheid2, Sakandar Rauf2*,
and Krassen Dimitrov2
87
52. The significance of chlamydia trachomatis IgG antibodies in the prediction for patients
with oviduct inflammatory disease
Guo Yanjun*, Ye Yang, Xu Ying, and Zhang Hongxia
88
Animal Reproduction Institute, Key Laboratory for Conservation and Utilization of Subtropical
Agro-Bioresources, Guangxi University, Guangxi 530004, China
1)
SooAm Biotech Research Foundation, Kyunginro 64, Guro-gu, Seoul, Korea; 2)Department of Animal Sciences,
Chungbuk National University, Cheongju, Korea; 3)College of Veterinary Medicine and Animal Medical Institute,
Chonnam National University, Gwangju, Korea
1)
Department of Animal Science, Division of Applied Life Science, Gyeongsang National
University,
Jinju 660-701, Gyeongsangnam Province, Republic of Korea; 2)Institute
of Agriculture and Life Science,
Gyeongsang National University, Jinju 660-701,
Gyeongsangnam Province, Republic of Korea;
3)
Animal Biotechnology
Division, National Institute of Animal Science, Suwon 441-706, Gyeonggi
Province, Republic of
Korea
1)
SooAm Biotech Research Foundation, Kyunginro 64, Guro-gu, Seoul, Korea; 2)Department of Animal Sciences,
Chungbuk National University, Cheongju, Korea
1)
Department of Biotechnology, School of Biotechnology, International University, Vietnam National University,
Ho Chi Minh City, Vietnam, 2)School of Chemistry and Molecular Biosciences, The University of Queensland,
St. Lucia, Queensland, Australia, 3)Australian Institute for Bioengineering and Nanotechnology (AIBN) Corner College
and Cooper Roads (Bldg 75), The University of Queensland, St Lucia, Queensland, Australia
1)
Department of Anatomy and Histology, Embriology, Medical College, Jiaxing, Zhejiang, China;
Department of Clinical Medicine, Medical College, Jiaxing, Zhejiang, China; 3)Department of Histology,
Embriology, Medical College, Jiaxing, Zhejiang, China; 4)Department of Human Reproduction Center, Maternity
and Child Care Hospital of Jiaxing, Jiaxing, Zhejiang, China
2)
53. Nitrate removal in a recirculating aquaculture system using a biological hybrid
configuration of free water surface constructed wetland and facultative anaerobic
biofilter with subsurface horizontal flow
Tran Cong Trung1* and Petch Pengchai2
1)
Quang Binh University, Quang Binh, Vietnam; 2)Environmental Engineering, Engineering,
Mahasarakham University, Mahasarakham, Thailand
89
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10th Annual Conference of Asian Reproductive Biotechnology Society 2013
xix
54. The rate of growth of tialpia (Oreochromis Niloticus Peters) reared in aquaria fed with
compounded fish diet out of holothuria rigida selenka and ipomea aquatic torks
Serapion N. Tanduyan*
90
Cebu Technological University 6050 San Francisco, Cebu, Philippines
55. Real-time monitoring of wine fermentation using disposable, interdigitated
gold microelectrodes
Yen T. K. Dang1* and Loc T. Nguyen2
91
1)
School of Biotechnology, International University, Vietnam National University, Ho Chi Minh City, Vietnam
Department of Food Technology, School of Biotechnology, International University, Vietnam National University,
Ho Chi Minh City, Vietnam
2)
56. Optimization of culture conditions for cellulase production on bagasse by anaerobic bacteria
Bui Thi Thien Thanh, Vo Ngoc Thao Nguyen, and Vo Van Song Toan*
92
57. Determination of the effects of acid citric supplementation and nitrogen-phosphorus
deficiency on the lipid accumulation of Nannochloropsis sp.
Nguyen Dan Van* and Nguyen Huu Hiep
93
58. Isolation and identification of bacillus subtilis from mud-dreg and waste water of
beer production
Nguyen Minh Thuy and Tran Nhan Dung*
94
59. Production and optimization of guava wine
Pham Hong Quang* and Ha Thien Huong
95
60. Yield and antibacterial activity of Ganoderma Lucidum on bagasses and sawdust substrates
Bui Thi My Huong, Tran Non Nuoc*, and Tran Nhan Dung
96
61. Design specific primers for the detection of the entomopathogenic fungus
METARHIZIUM NISOPLIAE VAR. ACRIDUM by PCR technique
Truong Khanh Anh Co and Tran Thi Xuan Mai*
97
62. Isolation of high yield phosphate solubilizing bacteria associated with sugarcane
Nguyen Thi Hong Phuong and Nguyen Huu Hiep*
98
63. Effect of thyroid hormones on porcine oocyte maturation in vitro
Walker E.*, Vassiliev I., Mclfatrick S., and Nottle M.B
99
Biotechnology Research and Development Institute, Can Tho University, Can Tho, Vietnam
Biotechnology Research and Development Institute, Can Tho University, Can Tho, Vietnam
Biotechnology Research and Development Institute, Can Tho University, Can Tho, Vietnam
Biotechnology Research and Development Institute, Can Tho University, Can Tho, Vietnam
Biotechnology Research and Development Institute, Can Tho University, Can Tho, Vietnam
Biotechnology Research and Development Institute, Can Tho University, Can Tho, Vietnam
Biotechnology Research and Development Institute, Can Tho University, Can Tho, Vietnam
Reproductive Biotechnology Group, School of Pediatrics and Reproductive Health & Centre for Stem Cell
Research, Robinson Institute, University of Adelaide, Adelaide, South Australia, Australia