Biology 304H Histology Course Outline Fall 2007
Introduction and Course Objectives
Histology is not just the study of tissues; it is a field of research where anatomy
and physiology can be visualized at the cellular level. Through observation of the
four basic tissue types, the way in which these four types are integrated to form
organs and how organs are combined to form systems, this course will introduce
the physical description of the normal at the light microscope level, while looking
at research examples and techniques used to investigate the abnormal.
In a scientific era where focus is turning to molecular biomarkers, new
fluorescence technology and other immuno/cyto/histo-chemical techniques have
rejuvenated the field of histology, thus enhancing our knowledge of
histopathology and pathophysiology for investigation in the medical, forensics,
biological, and toxicological fields. Combining histology with information gained
from electron microscopic investigation, as well as physiological and biochemical
evidence that relates to the integrative, we will introduce functional aspects that
exist between the cells in tissues, the tissues in organs, and the organs in
systems.
The histologist must not only be familiar with the appearance of the tissues, but
must also understand why we look at tissues. The aim of this course is not only
to provide an opportunity for becoming familiar with the appearance and
functional aspects of a number of different tissues, organs and systems, but will
also offer some practical learning experience in the technical aspects of histology
including: tissue processing, embedding, sectioning and staining, digitally
photographing, observing and describing results, while ultimately introducing the
application of histology in scientific research with the goal to generate more
questions…. what do you see?
Instructor:
Christina M.
Fridgen
Research Associate and
MSc. Candidate
Amphibian Toxicology
and Pathology
Lab Demonstrator:
Debbie Leitz
Histology and EM
Technician
Lecture
Laboratory
Textbook
Email: cfridgen@trentu.ca
Office: ES C103
Office Hours:
Tues: 9:00-17:00,
Wed. 13:00-17:00
Email: dlietz@trentu.ca ES
C203, ext. 7486
Wednesdays 10:00 – 11:50
SC 115
Both labs will be held on
ESC A205 and A209
Monday 13:00 to 15:50
Wheater’s Functional Histology, Fifth Edition, Churchill Livingstone
This syllabus, lecture objectives and notes, laboratory outlines, project
description, the microtoming project outline and methods, as well as
announcements and other course materials will be posted on Webct .
Please be sure to check routinely for updates.
Examination: Will cover both theoretical and practical aspects of the course and
will be held during the formal examination period.
Marking Scheme
Submitted project*
Quizzes (6x5%)
Mid Term
Final Exam
20%
30%
20%
30%
Projects: Each student must submit a completed project as part of the
requirement for completion of the course on or before the due date December
7th, 2007. Satisfactory completion of this component will include:
• Tissues that have been sectioned, stained, and mounted on 5 clearly labeled
slides must be submitted with the with best slide indicated for evaluation. Each
student must independently perform all technical steps.
• The write-up, as well as the presentation of micrographs and illustrations
generated from your own slides, will be done following appropriate journal format.
At least 3 references to primary research must be used in addition to other
literature cited. Examples are available in the lab and should be consulted as a
guide.
Submission of Assignments and Late Submission Policy: Assignments are to be
handed in to Debbie Lietz ES C203 by 4:00 p.m. on December 7, 2007.
Assignments handed in after the due date will be penalized at the rate of 5% per
day. Assignments submitted after Dec 14, 2007 will only be accepted for grading
with a documented excuse and following a meeting with the course instructor.
The lecture topics are given in the schedule below. It is highly recommended that
students read pertinent chapters from the text before attending the lectures.
Lab Topics will correspond to and follow as closely as possible, the lecture topics
–with the lab content being covered the week after lecture. Laboratory Guides
will be made available to you through WebCT.
BRING TEXT BOOKS AND PRINTED LAB GUIDE TO LABORATORY SESSIONS!!!!
Short quizzes will be held at the end of the laboratories as noted below to test
your new knowledge –content accumulated from both the previous lecture and
the current laboratory material. There will be no make-up quizzes, and the results
from your best 6 will be used to calculate your final grade.
BIOL 304H, HISTOLOGY SCHEDULE 2007
Week
Lab (Monday)
Lecture (Wednesday)
-Microscope review
-Introduction to Histology
-Intro. to histological
-Developmental Histology
analysis (text Ch. 1,
-Histological study at Trent
appendix 2)
-Intro. to Project
Project Lab:
-Cell Organization
Week 2
-Rat dissection
-Epithelial Tissue
(Sept 17-21)
-Microtoming safety
-Muscle Tissue
and techniques
-Respiratory system
Ch.5: Epithelial Tissue -Connective Tissue
Week 3
Ch.6: Muscle Tissue
-Nervous Tissue
(Sept 24-28)
Ch.12: Respiratory
-Central Nervous System
System
QUIZ 1
Ch.4:Connective
Review
Week 4
Tissue
-Cellular Form and Function
(Oct. 1-5)
Ch.7: Nervous Tissue
-Intro. to Histopathology
Ch.20:CNS
QUIZ 2 -Immune System
Thanksgiving
-Circulatory system
Week 5
No lab!!
-Blood
(October 8-12)
Ch.8: Circulatory
Guest lectures:
Week 6
Histology Research at Trent
(October 15-19) system
Ch.3: Blood
QUIZ 3 (details - to be announced)
Week 7 (October 22-26) ---------- Reading Week --------------------Skin
Week 8
-Special Sensory organs
(Oct 29-Nov 2)
Mid-Term
-Oral tissues
Ch.9: Skin
QUIZ 4 Gastrointestinal System
Week 9
Ch.21:Special Sensory
(Nov. 5-9)
organs
Ch.13: Oral tissues
Ch.14:
QUIZ 5 Urinary System
Week 10
Gastrointestinal
System
(Nov. 12-16)
Ch.16:
QUIZ 6 -Liver and Pancreas
Week 11
Urinary System
-Skeletal System
(Nov. 19-23)
Ch.15:
QUIZ 7 Endocrine System
Week 12
Liver and Pancreas
(Nov. 19-23)
Ch.10: Skeletal
System
Ch.17:
QUIZ 8 Reproductive systems
Week 13
Endocrine Glands
(Nov. 26-30)
Lecture
Readings:
Week 1
(Sept 10-14)
Ch 5
Ch 6
Ch 12
Ch 4
Ch 7
Ch 20
Ch 11
Assigned
readings
Ch 3
Ch 8
Ch 9
Ch 21
Ch 13
Ch 14
Ch 16
Ch 15
Ch 10
Ch 17
Ch 18
Ch 19
Week 14
(Dec. 3-7)
Ch.18, 19:
QUIZ 9 Last Lecture:
Reproductive Systems Review and Surprise??
Laboratory Workbook Requirements: Each student is encouraged to maintain a
laboratory workbook that will include representative drawings of the tissue
studied in the laboratory. The primary purpose of such a requirement is to ensure
that the student has an ongoing reference of the laboratory material that can act
as a study aid for the exams.
Academic Dishonesty
Academic dishonesty, which includes plagiarism and cheating, is an extremely
serious academic offence and carries penalties varying from failure in an
assignment to suspension from the University. Definitions, penalties, and
procedures for dealing with plagiarism and cheating are set out in Trent
University’s Academic Dishonesty Policy which is printed in the University
Calendar. (To be added, if applicable: Departmental interpretations of this policy
are posted on the departmental website).
Access to Instruction
It is Trent University’s intent to create an inclusive learning environment. If a
student has a disability and/or health consideration and feels that he/she may
need accommodations to succeed in this course, the student should contact the
Disability Services Office (BL Suite 109, 748-1281, disabilityservices@trentu.ca)
as soon as possible. Complete text can be found under Access to Instruction in
the Academic Calendar.