Welcome to Biology 523: Histology
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Side 1: large print--> first name & last initial
Side 2:
name:
major:
career goal:
your hopes for the course:
information about you that I should know:
Biology 523: Histology, 4 credits
Instructor:
Dr. Elizabeth Connor
353 Morrill IV South
telephone: 545-4855
email: econnor@bio.umass.edu
office hours: by appointment
ANNOUNCEMENTS
Flu can include: fever, cough, sore throat, runny or stuffy nose,
body aches, headache, chills, and fatigue
Preventing flu
• Get vaccinated.
•
Wash your hands often.
• Cough or sneeze into a tissue or your elbow instead of into your hands.
• Avoid touching your eyes, nose or mouth, to reduce the spread of germs.
• Don't share food, drinks, utensils and other similar items.
• If you’re sick, stay home!!! Email me about your situation and we will
work it out.
OBJECTIVES
#1- ANATOMY: To understand the structural
basis for the functioning of a living organism
Close relationship between structure and function
GROSS ANATOMY (macroscopic)
ANATOMY
HISTOLOGY (microscopic)
OBJECTIVES
#2- TECHNICAL ADVANCES: To appreciate that
our ability to answer questions is driven by the
development of new technology
Why study histology?
What are the uses of histological techniques
and microscopic tissue identification?
Tools of the Histologist
Requirements:
Tissue Preparation
Start with an organism:
Organ->Tissues-> Cells-> Molecules
fixation/sectioning/ staining
Imaging (Microscopy)
Interpretation
- cell biology
- physiology
- structure of tissues
- organ structure/function
Visual Integration
**A challenging skill**
Teaching Assistants:
Bryan Olson
bolson@nsm.umass.edu
Undergraduate Lab Assistants
Required Textbook:
Histology: A Text and Atlas, Ross, Kaye,
and Pawlina
6th Edition, Lippincott Williams & Wilkins
ISBN 0-683-30242-6
*** BRING TEXTBOOK TO LAB ***
Many free online histology
materials are available!
Jejenum
Links on Course Webpage
Recommend Histology World
Recommend MedPics UCSD
Course Web Page
• Class Preparation Page: Announcements
• Reading Assignments
• Syllabus
• Class Notes: pdfs and ppt of class presentations
• Laboratory: syllabus, lab exercises, project guides,
online tutorials, useful links
• Review Materials
- Review questions
- Sample Exams and Answers
- Animations and useful links
Grading: 300 total possible points
Two 1 hour in class exams (55 points each)
110
Final Exam
60
Homework Problems
10
Laboratory Attendance
15
Three Laboratory Practical Exams
( 15, 15, 25 )
55
Contributions to Images2012 Gallery
20
Group Oral Project Presentation
10
Individual Written Project Report (2 parts)
20
Laboratory Experience (120 Points)
Exercises using Prepared Slides
Assessment: Three Practical Exams (15, 15, 25 points)
Group Lab Project #1
As a class, we will assemble Images2012, an on-line image
gallery of tissues, cells and organs examined during the
course. (20 points)
>2500 images from previous class efforts also available on our
course website
Assessment: Evaluation of postings to Images2012 (20 pts)
Accuracy, labeling, quality of image
Group Lab Project #1
Images2012 Gallery: Your work is protected by“Please Note: The imagery created by students in this course is licensed under the
Creative Commons Attribution- NonCommercial - ShareAlike 3.0 Unported License. To
view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/ or
send a letter to Creative Commons, 444 Castro Street, Suite 900, Mountain View,
California, 94041, USA.”
Laboratory Experience (120 Points)
Group Lab Project #2
Sectioning and staining of
frozen and paraffin-embedded tissue
of a rat organ.
Techniques: microtome sectioning, frozen sectioning with
cryostat, staining, immunohistochemistry, fluorescence
microscopy, bright field microscopy, imaging, image
processing.
Assessment:
Group Oral Presentation: In class (10 pts)
Individual Written Project Report: (20 pts)
First lab:
Tuesday, January 31st or
Wednesday, February 1st
To prepare for labRead Lab 1: Microscopy and Imaging
Think about project partners (3/group)
Secrets of Success
-Attend every class and every lab.
-Come to class and lab prepared.
-Ask questions.
-Complete all assignments.
-Review & study material after each lecture.
-Answer review questions.
-Attend help and review sessions.
-Identify study partners.
Course Website!
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ACCESS TO ISB 264
Exceptions: it will be in use by other courses on:
Monday and Thursday 1:25pm-4:25pm
Friday 3:35pm-4:35pm
……….And some other times to come
Challenge:
Identify tissues. (Epithelial, Nervous,
Muscle, Connective Tissue)
Identify composite of tissues as an organ.
Use appropriate descriptive language and terms.
Understand the appropriate use of techniques to
study material at the histological level
Orientation and Plane of section
CROSS SECTION
Orientation and Plane of section
LONGITUDINAL SECTION
Challenges of Interpreting Plane of Section
= Organ
= Surrounding Tissues
Challenges of Interpreting Plane of Section
= Organ
= Surrounding Tissues
Staining techniques
Magnification, Details of structure