02. Endocrine Practical 1
– Glucose Tolerance Test
(GTT)
Degree: BMed2406
v2.0 Haydn Allbutt (28/7/15)
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02. Endocrine Practical 1 – Glucose Tolerance Test (GTT)
Degree:
BMed2406
Convener:
Isabel Arnaiz
Date and time of Classes (for 2015):
 Mon 3/8/2015 9am-1pm CPC FDS 1 (9-1) & Learning Studio (9-12) + FDS 2
(12-1)
 Wed 5/8/2015 9am-1pm CPC FDS 1 & 2
 Thur 6/8/2015 9am-1pm CPC FDS 1 & SEM 2.02
 Fri 7/8/2015 8am-12pm CPC FDS 1 & 2
No. of Classes:
8; two classes run simultaneously over 4 days
No of students per class:
Number of students
(Class 1)
Number of students
(Class 2)
Monday
~35
Wednesday
~30
~35
~30
Thursday
~36
~35
Friday
~23
~23
Students working in groups of: Each student takes their own measurements
individually
Class Summary
(Class notes currently unavailable, will fill this in when they are posted again)
This class allows the students to practice performing a glucose tolerance test using
a 50g glucose drink in preparation for the next class.
Special Notes for class
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Each student will need a laptop
When the class is held in FDS 1 & 2 simultaneously (ie Wed 5/8/15 and Fri
7/8/15) the dividing doors should be shut
We currently use the Accu-check performa blood glucose monitors. The test
strips for these are approximately $49.69 for a box of 100 strips. Each
student tests their blood 12 times over the Endocrine 1 and Endocrine 2
class (6 times per class; following the protocol used by Jennie Brand-Miller’s
lab to determine the glycemic index so 6 samples are need), therefore cost of
blood strips is ~$6 per student. – Check with adel where he currently gets
them and if this is the cheapest
Not all students will participate. At the moment the number of consumables
specified for this class assume all enrolled students will participate. It is
alright to do that for this class as the glucose drink has a long shelf life.
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Each enrolled student will need a 50g glucose drink (volume not important,
current drink is 50g in 300mL) – so will ned 247 glucose drinks for BMedSci
in 2015
Need 2 x urine test strips per student (494 in 2015), students test urine
glucose at T=0 and T=1hour.
Find out from adel where he gets the Urine test strips to put here
Check we have spare batteries for blood glucose meters - What sort of
battery do we need?
Staff Needs
o Friday 5pm: Put gear on trolley ready for Monday morning – CPC staff
will get drinks from basement
o Monday 8am: setup class
Equipment Setup
General in Class
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Bio waste bins – for blood test waste
A4 sized number from 1 to 6 to use as station numbers with the name of the
station.
Signs telling students where and how to dispose of each type of waste.
Signs needed
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(flat) “Don’t dab blood everywhere” – for each table
(Flat) Isabel’s waste disposal sign – for each table
2 x “Station 1 – Consent Form” – one for each room
2 x “Station 2 – Urine Testing Station” – one for each room
14 x “Station 3 – Glucose Tolerance Test (GTT)” – one for each table where
students will be doing blood test
2 x “Station 4 – Glucose Load” – one for each room
2 x “Station 5 – Diagnostic Test for Pregnancy” – one for each room
(flat) 2 x “ Read off the Urine test strip from the bottle below and discard the
used strip into the biohazard bin before returning to class”
Station 1 – Consent Form
Table at the front of the room to have appropriate number of copies of the consent
form.
Station 2 – Urine Testing Station
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Plastic disposable containers
Urine test strips
Instructions on how to use the urine test strips
A sign outlining the name of the station that
can be seen by the students from far away
A sign of the chart for interpreting test strips
up on the wall of both man and woman
toilets.
A biological waste bin just outside the toilets.
Urine Test Strips (glucose only)
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Station 3 – Oral Glucose Tolerance Test (GTT)
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14 x bench coat covers
Cover each table in bench coat
Masking tape to fasten covers
Automatic Blood lancets (each student will do 6 blood tests so will need 48
lancets per table of 8 students)
Alcohol wipes (a bunch for each table)
Accu-chek Performa glucometer (will need spare batteries)
Test strips for above (each student will do 6 blood tests so will need 48 blood
strips per table of 8 students – students will test incorrectly and so will need
extra strips)
Yellow sharps bins
A sign outlining the name of the station that can be seen by the students
from far away.
Tissues
Instructions on how to use glucometer
Lancet
Glucometer with test strip
Station 4 – Glucose Load
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A table at the front that contains Glucose drinks containing 50g glucose in
300 ml. (1 per student)
Jugs with drinking water for those that want it
Small Plastic Cups
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Station 5 – Diagnostic Test for Pregnancy
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Put out two test kits per room one for each type of urine
“Forelife” Professional test kits (ivankas@marcodpolo.com.au).
Artificial Urine samples (with hcg and without)
Instructions for using test kits
A sign outlining the name of the station that can be seen by the students
from far away
Instructions for making up artificial Urine
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Colour ringers solution with yellow dye or methylene orange and picric acid
You then make two samples
o Urine A: Pregnant Urine (positive sample) – with HCG [500IU per
100ml; Sigma sells a 1000IU vial (C0684)]
o Urine B: Non-pregnant urine (negative sample) – without HCG
Layout of Equipment in the Class
See A3 pdf class layout map for how to set up the equipment
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