Components of Living Things Notes 2/29/2008 10:03:00 AM DO NOT PRINT THIS DOCUMENT. IT IS TOO LONG. element: a substance that cannot be further broken down by a chemical reaction (there are 92 naturally occurring elements) compound: combination of elements in a fixed ratio (ex NaCl one sodicum atom and one chlorine atom) “C, O, H, N make up 96% of living matter” Campbell, 1996. Carbohydrates 1) What is the role of a carbohydrate (sugar) in a cell? Main use for energy/fuel and for building structures with in a cell or organism (goes through glycolysis to harvest ATP to power the) Role of carb at cellular level: to help build structures within the cell, to produce energy by going through the path of glycolysis and producing ATP – high energy molecules to fuel the cell so it can carry out all of its proceses 2) What is the role of a carbohydrate (sugar) in an organism (living thing)? To provide energy/fuel for the organism, to provide nutrients for the organism to live, some cells require only carbohydrates to do their jobs – and can only use carbohydrates (sugars) for the energy to do their jobs Role of carb at the organism level: to power the entire organism – give it energy and matter to build different tissues and structures within the organism’s body 3) What is the chemical make up of a carbohydrate? What elements are in it? Carbon, hydrogen, oxygen in fixed ratios Ex: Glucose is a hexose sugar (6 carbon sugar) C6H12O6 Ribose is a pentose sugar (5 carbon sugar) C5H10O5 4) What does the chemical structure of a carbohydrate look like? How are the elements bonded together? Glucose ball and stick model http://staff.jccc.net/PDECELL/biochemistry/glucose.gif Structural formula of glucose http://www.palaeos.com/Fungi/FPieces/Images/Glucose.gif Lactose: http://www.drugdeliverytech.com/cufm/jpg/002175.jpg Where can you find carbohydrates? Carbs come from: http://www.faqs.org/nutrition/images/nwaz_01_img0048.jpg and: http://newsletter.wsu.edu/eplay/user_pics/375x250_veggies.j pg and from: http://www.fruitforyou.com.au/fruit-hampers- melbourne/corporate-fruit-box.jpg Carbohydrates are made of carbon, oxygen, and hydrogen in fixed ratios Carbs are found in cell walls of plants, in the mitochondria going through the process of glycolysis to harvest the energy from the carbohydrate, carbs are located on the surface of cells and used in cell surface recognition, 5) How does a living organism obtain carbohydrates? Does an organism need to eat carbs in order to obtain it? An organism either eats a plant/fruit/starch/or other organism that has ingested carbohydrates. If you are a plant, you make your own carbs through the process of photosynthesis. If you are a human you need to eat things that contain carbs. 6) Do any living things produce carbohydrates? Yes, plants, and other photosynthetic algaes, plankton etc 7) What is glucose? Simple sugar Carbohydrate – sugars – simple and complex Glucose is a simple sugar ( monosaccharide – a single sugar) 8) What is starch? Starch: http://www.elmhurst.edu/~chm/vchembook/images/547starch.gif starch granules: http://www.public.iastate.edu/~pkeeling/startype.jpg starch: storage polysaccharide found only in plants; starch is made of glucose monomers strung together ; plants store starch in granules inside organelles like plastids and chloroplasts animals have enzymes that can break down starch into glucose monomers and use the glucose for energy 9) What is cellulose? cellulose: structural sugar in plants – used to make cell wall, cellulose is also known as fiber, which humans cannot digest 10) What is chitin? Chitin: polysaccharide made by arthropods (insects, spiders etc) to make their exoskeleton – their hard crunchy case chitin surgical thread: thread made of chitin used in surgery to stitch wounds b/c the thread will eventually decompose and does not need to be removed 11) What is a mono, di, polysaccharide? Mono- simple sugar (ex glucose) Disaccharide – 2 monosaccharides linked together by a dehydration reaction (condensation reaction) (lactose) Polysaccharide – a bunch of monosaccharides linked together to form a chain of sugars (starch), glycogen, cellulose, chitin Proteins What is the role of protein in a cell? Proteins control almost everything in a cell and in an organism. Proteins are responsible for moving things in a cell, for controlling the chemical reactions within a cell, for controlling cellular processes (like glycolysis), for allowing cells to be recognized by other cells, for copying DNA. What is the role of protein in an organism? To build tissues and organs, to control the movement of things throughout the body, to control metabolic reactions, to control chemical processes, to store energy for the body, they help w/ all biological processs, they are catalysts, transport other molecules, store molecules, provide support for the cell and organism, they provide defense (immune system), they help w/ movement, they help w/ nerve impulses, growth, they do almost everything in a cell or an organism What is the chemical makeup of a protein? What elements are proteins made of? What does a protein look like? How is a protein bonded together? What is the chemical structure of a protein? Proteins made from the 20 amino acids. Amino acids are the monomers (single unit, building block) of a polypeptide chain (polymer – multiple monomers linked together). Proteins fold and created a 3D structure based upon the amino acids in the chain, structure and function based on the amino acid sequence and 3d Structure Basic structure: central carbon w/ a hydrogen, carboxyl gp (COO or COOH), amine gp NH3, and then a side chain ® that varies ex: http://www.hcc.mnscu.edu/programs/dept/chem/V.27/amino_acid _structure_2.jpg So an amino acid has nitrogen, carbon, hydrogen, oxygen, and then elements that compose the side group – which are made of carbon, hydrogen, oxygen, sulfur, nitrogen, Bonded together by single covalent bonds, and some side groups have double covalent bonds Here are the 20 amino acids: http://upload.wikimedia.org/wikipedia/commons/thumb/c/c5/Am ino_acids_2.png/600px-Amino_acids_2.png A protein goes through 4 steps before becoming an actual protein. It starts with linking the amino acids together into a polypeptide chain, and then the chain folds in different ways, and ends up creating a 3D structure that has a specific function. http://cropandsoil.oregonstate.edu/classes/css430/lecture%2 09-07/figure-09-03.JPG Hemoglobin is a protein in your body that is in red blood cells and transports oxygen to the rest of your body. It has a specific 3D structure that fits its function http://www.nlm.nih.gov/medlineplus/ency/imagepages/19510.ht m Lactose intolerance: lactose is the sugar found in milk (yes there is sugar in milk! Remember lactose is a disaccharide, which means it is made of two simple sugar compounds chemically bound together). If you are lactose intolerant then you have a deficiency in the enzyme (protein) lactase which breaks down lactose for your body to process Here is the computer generated image of lactase http://160.114.99.91/astrojan/protein/pictures/lactase6.jpg Where can you find protein in an organism? Everywhere! In every cell, all over the body, doing a specific job. How does a living organism obtain protein? Does a living organism make protein? Most microorganisms (like E. coli) can make all 20 amino acids through various complex chemical reactions. Humans cannot make 9 of the 20 amino acids, and must get those 9 amino acids from consuming food. When you consume protein (eating a steak) your body digests the protein and breaks it down into amino acids. Then your body can use those amino acids to make proteins to work in the cells in your body. Proteins that already exist in your body are constantly broken down and the amino acids are reused in the synthesis of new proteins. A protein is tagged with a specific “label” (ubiquitin, a protein) if it needs to be broken down into its amino acid components. In order to make the amino acids, our bodies/cells have to obtain nitrogen from a molecule of ammonia. The carbon in amino acids comes from breaking down carbs and having carbs go through different biochemical pathways So some organisms make all their protein, some organisms need to eat protein and break it down to synthesize the proteins their bodies need What is an amino acid? An amino acid is the monomer (most simple part) of a protein. Amino acids are the building blocks of proteins. They are made of an ammonia group linked to a carbon and other elements into a compound. An amino acid bonds to another amino acid to form a dipeptide (or 2 amino acids linked together). A polypeptide chain is a chain of amino acids linked together by chemical bonds. There are 20 different amino acids. They have a basic structure in common, but they differ from each other in one of their side chains, which is made up of different elements in different combinations to give the amino acid its specific characteristics. An enzyme is a protein with a specific job. Enzymes are catalysts, which means they are used to speed up the process of a chemical reaction in your body. They are responsible for many of the metabolic chemical reactions in your body. They help break down your food and get the energy out of your food for your body to use. How are proteins used in cellular transport? How are proteins used in movement? They are used to move molecules and compounds throughout the cell and throughout an organism’s body. For example, hemoglobin is a protein. It helps transport oxygen to all the parts of your body. How are proteins used in defense (immune system)? Some proteins (lysozymes) are enzymes that break down invasive bacteria in your body, Lipids Lipids don’t like water, mostly nonpolar in structure, mostly made of hydrogen and carbon linked together 3 families within the lipid category are: fats, phospholipids, steroids What is glycerol? Glycerol is a hydrocarbon with an OH group attached to it. It looks like this: http://www.burns-petnutrition.co.uk/Images/TRIGLYCERIDE.gif Glycerol links to a fatty acid. A fatty acid looks like this (see below) and when 3 fatty acids link to a glycerol molecule (by forming covalent bonds) a triglyceride is formed. A triglyceride (triacylglycerol) is what we know as a “fat”. http://www.oliveoilsource.com/images/triglyceride.jpg Saturated – refers to that there are no double bonds in the hydrocarbon chain of the fatty acid Unsaturated: means there is one or more double bonds in the hydrocarbon chain http://pirate.shu.edu/~rawncarr/molmodel/fattyacids.jpg Animal fats are saturated http://www.dairy.com.au/consumers/images/dairyaustralia/agd a_07_tatura_butter.jpg Vegetable fats and fish fats are unsaturated (oils found at room emp that are liquid like canola oil, olive oil, cod oil) http://www.antoniocelentano.com/images/group%20small%20web. jpg Hydrogenated vegetable oil means that a fat that is normally unsaturated has been chemically changed to be a saturated fat by adding more hydrogen to the hydrocarbon tail of the fatty acid What is the role of fat in a cell/organism? Role of fat: to store energy, remember 1 g of fat has 9 cal of energy for your body to use, to cushion organs, to insulate your body, to use as a chemical messenger for reactions, Where can you find lipids in an organism? In every cell, tissue, in cell membranes, in fat cells that store the fat as energy for use later, in fat tissue that cushions your organs, fat forms a layer under your skin to insulate your entire body, steroids are used as chemical messengers throughout your body, What is a phospholipid? Made of only 2 fatty acids attached to glycerol, and a phosphate group is attached to glycerol too The “head” of the phospholipids likes water b/c of its chemical structure, the “tail” of the phospholipids do not like water because of their chemical structure. Phospholipids are used to make cell membranes. What is a steroid? Steroids are lipids that are made of chemical ring structures. Cholesterol is an example of a steroid. Steroids are used as chemical messengers that transfer messages throughout the cells and body. http://www.abc.net.au/health/library/img/cholesterol_struct ure.gif How does an organism obtain lipids? They eat other things that have lipids (we eat fatty foods) but an organism can also synthesize lipids, especially phospholipids and steroids. Nucleic acids What is a nucleic acid? Nucleic acid is the general term for the compounds DNA and RNA (deoxyribonucleic acid and ribonucleic acid). What is heredity? Heredity is passing a genome (the entire set of genes of an organism) from one generation to the next generation. You inherited your genes from your parents. What is a gene? Genes are made of DNA – they are a unit of inheritance. They are the directions for making proteins and for making you. What is a genome? The entire set of an organism’s genes. What is DNA? Deoxyribonucleic acid (the instructions for life – the genetic material that contains all the directions for programming everything in every cell, in an entire organism. http://www.biologycorner.com/resources/DNA-colored.gif http://ocw.mit.edu/NR/rdonlyres/Biology/7-A12Fall2005/D4134A30-F348-4615-8B0A-D0CB5ED86081/0/chp_dna.jpg What is the purpose of DNA? DNA is the genetic material that you get from your parents and makes you, you. It codes for synthesizing all the proteins and structures your cells and your body needs to function. DNA controls the cell and its processes. It is made of NUCLEOTIDES. What are nucleotides? The building blocks for DNA and RNA. There are 4 different nucleotides. They have a pentose sugar (a sugar w/ 5 carbons) and the sugar is called deoxyribose. It has a nitrogenous base (a structure made of nitrogen bound to other elements) and it has a phosphate group (phosphorous and oxygen bonded together). There are 4 different nitrogenous bases: cytosine (C), thymine (T) adenine (A), and Guanine (G). http://www.chemsoc.org/ExemplarChem/entries/2003/imperial_B urgoine/nucleotide.jpg http://www.viewingspace.com/genetics_culture/images_genetic s_culture/gc_assign/nucleotide2.gif http://www.nvo.com/jin/nssfolder/scrapbookcell/4%20nucleotide.jpg What is RNA? What is the purpose of RNA RNA is another type of nucleic acid. mRNA is used to transfer the message from DNA to a machine that will translate the message into protein, that will then have a job in your body. mRNA is a messenger (m = messenger). Where are DNA and RNA found? In the nucleus of a cell. The nucleus of a cell is like a control center – it controls all the activities and processes of a cell. (A cell is the smallest unit of life.) Cycles What is the nitrogen cycle? http://www.biocrawler.com/encyclopedia/Nitrogen_cycle Like every natural element, there is a fixed amount of nitrogen available on our planet. We don’t “run out” of nitrogen because there is a very specific cycle that it follows, and it is constantly being recycled through this system known as the “nitrogen cycle”. Living organisms require nitrogen to synthesize the molecular compounds in their bodies, especially nucleic acids and proteins. You do not need to memorize the cycle. You just need to understand that nitrogen is constantly being used in different forms by different organisms. http://www.cst.cmich.edu/centers/mwrc/nitrogen_cycle1.gif What is the carbon cycle? http://eo.ucar.edu/kids/green/cycles6.htm http://www.kidsgeo.com/images/carbon-cycle.gif Just like the nitrogen cycle, carbon is recycled too. We have a fixed amount of carbon on earth, and so it is always cycling through this cycle. Why is water important for living organisms? Water is involved in many chemical reactions within an organism. It is necessary for plant growth, it is necessary for all the organisms that live in an aquatic or marine environment. It is necessary for you to have so your body functions properly. Your cells need water. Water supplies fluid for your blood, for many different things to work internally in an organism. What is organic chemistry? It is the study of compounds containing carbon and hydrogen. It was thought that organic compounds were necessary for life, and they are, but life requires other compounds as well. Many organic compounds, such as nucleic acids, carbohydrates, lipids, and proteins are necessary for life. Why are the following elements important for life: carbon, oxygen, hydrogen, nitrogen, phosphorus, and sulfur? Most of the compounds that exist – that make up the cells, tissues, organs, systems in an organism consist of those elements. Carbon, hydrogen, oxygen, and nitrogen make up 96% of all living matter (Campbell, 1996). P, S, Ca, K, and a few other elements make up the remaining 4% of an organism (Campbell, 1996). 2/29/2008 10:03:00 AM 2/29/2008 10:03:00 AM