ChE 4552 Introduction to Biotechnology
Instructor: 胡育誠 (Andy Hu)
Office: 307 (x33642)
Email: yuchen@che.nthu.edu.tw
ychu@mx.nthu.edu.tw
Web: http://www2.che.nthu.edu.tw/biohu/
Classroom: 221
TA: 林欣怡 鍾沐恩 (327, 329)
Course contents: The topics of this course cover basic cell biology, fundamental
genetic engineering, cell culture, bioreactor design, downstream bioprocesses and
applications of biotechnology.
Textbook:
1. Colin Ratledge, Bjorn Kristiansen. (2006) Basic Biotechnology. Third Edition.
Cambridge University Press, Cambridge, UK.
Reference:
1. Shuler ML and Kargi F. (1992) Bioprocess Engineering: Basic Concepts. Prentice
Hall International, London.
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3.
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6.
Watson, JD, Gilman, M, Witkowski, J, Zoller, M. (1992) Recombinant DNA.
2nd Ed. W.H. Freeman and Co., New York.
Karp, G. (2002). Cell and Molecular Biology: Concepts and Experiments. 3rd Ed.
John Wiley &Sons. New York.
Barnum, S.R. (2005) Biotechnology: an Introduction. 2nd Ed. Thomson Learning,
New York.
Voet, D., Voet, J., (2004) Biochemistry. 3rd Ed. (Vol.1) John Wiley & Sons. New
York.
Glick BR and Pasternak JJ. (2003) Molecular Biotechnology: Principles and
Applications of Recombinant DNA. 3rd ed. ASM Press, Washington, D.C.
7. Alberts, B., Johnson, A., Lewis, J., Raff, M., Roberts, K., Walter, P. (2002)
Molecular Biology of the Cell, 4th Ed. Garland Science, New York.
8. Doran, PM (1995) Bioprocess Engineering Principles. Academic Press. San
Diego.
9. Blanch, HW, Clark, DS. (1997) Biochemical Engineering. Marcel Dekker. New
York.
10. Walsh, G. (2002) Proteins: biochemistry and biotechnology. John Wiley & Sons.
New York.
11. Nester, EW, Anderson, DG, Roberts Jr. CE, Pearsall, NN, Nester, MT. (2001)
Microbiology, A Human Perspective. McGraw Hill. New York.
12. Freshney, RI. (2005) Culture of animal cells: a manual of basic technique.
Wiley-Liss, New York.
Grading policy:
 Mid-term exam: 30%
 Final Exam: 50%
 Course participation: 10%
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Quiz: 10 %
Midterm exam: 11/18 (Tue)
Final exam: 1/13 (Tue)
Syllabus
Basics
 Chap1 Public Perception of Biotechnology
 Chap2 Overview of Biological Systems
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Chap3 Growth and Metabolism
Chap4 Genetic Engineering-Procaryote
Chap5 Genetic Engineering-Yeast and Fungi
Chap6 Culture of Animal Cells
Chap7 Microbial Process Kinetics
Chap8 Bioreactor
Chap9 Downstream Processing
Applications:
 Chap 14 Amino Acids
 Chap 15 Organic Acids
 Chap 16 Microbial Polyhydroxyalkanoates, Polysaccharides and Lipids
 Chap 18 Antibiotics
 Chap 20 Recombinant Proteins