Cell Division and Differentiation – Grade Ten Ohio Standards Connection: Life Sciences Benchmark B Explain the characteristics of life as indicated by cellular processes and describe the process of cell division and development. Indicator 4 Summarize the general processes of cell division and differentiation, and explain why specialized cells are useful to organisms and explain that complex multicellular organisms are formed as highly organized arrangements of differentiated cells. Lesson Summary: The purpose of this lesson is to teach students about mitosis, meiosis and cell differentiation and their purposes and implications in the development and functioning of multicellular organisms. Students will use individual journaling and a variety of hands-on activities summarized by group discussions. Understanding of the concepts will be demonstrated through construction of models of mitosis and meiosis and individual journal summaries. Estimated Duration: Five hours Commentary: Journaling allows students to reflect and make connections between physical models and the process of cell division and development. Constructing physical models and selfreflection may increase the learning and retention of the material. The use of portfolios or journal post-assessments provide an alternate means of assessment to meet the needs of a variety of learners. Pre-Assessment: Journaling: Have each student start a journal section for this lesson. Emphasize that this will not count as a grade at this point but will be used to assess the extent of their previous knowledge. Ask them to record answers to the following questions in their journal: 1. In your journal, draw a chromosome and indicate the location of a gene on the chromosome. 2. Skin cells are constantly being shed. How does your body replace these lost cells? 3. How are genes passed on from the cell of a father to his son? 4. How are many specialized cells in the human body generated from one fertilized egg? 1 Cell Division and Differentiation – Grade Ten Scoring Guidelines: Collect journals. Make positive comments on those who have correct answers, but do not assign a grade for this portion. Read and return journals before beginning instructional procedures below. Sample responses can be found in Attachment A, Student Journal Requirements. Post-Assessment: Throughout the pre-assessment and instructional procedures, students have been making observations and entries in their journals. Encourage students to include diagrams, flow charts, tables or other representations for clarity. A list of expected journal entries is located in Attachment A, Student Journal Requirements. Scoring Guidelines: Collect and assess individual student journals according to the guidelines in Attachment A, Student Journal Requirements. Pay special attention to summary sections of discussion questions. Example student responses are included with the attached grading rubric. Instructional Procedures: Part One: Cell Division 1. Ask for a volunteer to share his/her drawing of a chromosome by copying it on the board. Most students will depict a chromosome as a simple X, or maybe as a squiggly line. Use student ideas and input to correct any misconceptions and be sure students understand that the X shape is actually two chromatids (two copies of a chromosome), and that chromosomes only appear this way when undergoing cell division. 2. Allow a second volunteer to depict how a gene would appear on this chromosome. Explain that many individual genes are found on each chromosome and each gene carries information that may be expressed by coding for a protein. The remaining questions in the pre-assessment journaling exercise will be answered throughout the rest of the lesson. Instructional Tip: Do steps three through nine of the instructional procedures first with mitosis only, then repeat with meiosis only to minimize confusion. Concept Maps 3. Prepare sets of cards for students to use in this section. Draw or locate a simple diagram of each of the events of mitosis and meiosis. Put each diagram on a separate index card along with a brief description of the event. Label the back of each card with “Mt” (mitosis) or “Me” (meiosis), or use different colored cards for mitosis and meiosis. 4. Have students work independently, in pairs, or in mixed ability groups and give them the index cards describing the steps of mitosis/meiosis in separately labeled envelopes. Instruct students to first arrange mitosis cards in order. Allow about 10 minutes for students to put the events into a logical order based on what they already know about cells and cell division. Emphasize that this is not a graded activity, but to try their best 2 Cell Division and Differentiation – Grade Ten even if they do not know the answer; they may be surprised at how logical the steps will be. 5. Combine two groups (four students) to compare results. Instruct the group to tape its index cards on a poster board in the proper order. Display the posters on the chalkboard. Instructional Tip: The process of cell division is the main emphasis for the activity, and replaces rote memorization of the names of the phases. The terms prophase, metaphase, etc. should be used during instruction but not made into a memorization exercise. Some students may require one-to-one instructional support to understand these processes. Interactive Lecture 6. Go over the concept maps and put the steps of mitosis or meiosis in the correct order, talking through each step. Use one of the concept map posters as a model. If there are differences among the groups’ posters allow students to “vote” for first step, second step, etc. Arrange events in the correct order. 7. Discuss the life cycle of reproduction and development using humans as an example. A multicellular organism that is diploid (2n) develops gametes (egg or sperm cells) that are haploid (n). Fertilization occurs when an egg and a sperm cell (n + n) join to produce a zygote (2n). The cells of the zygote divide through the process of mitosis and they differentiate to form various parts of the body. Mitosis continues to promote growth and development. A multicellular organism is produced that is diploid (2n). Instructional Tip: There are many excellent online and conventional videos available that depict the processes of mitosis and meiosis. Using one would help students to understand the continuous flow of the processes from one step to the next. Show a clip of the type of cell division students have just finished mapping. Journal Entry 8. Have students take journal notes that emphasize the sequential logic inherent in the events of cell division. For example, the nuclear membrane must dissolve before the chromosomes can attach to spindles and line up. Later on, the nuclear membrane reforms after chromosomes have migrated to their new cells. The steps of mitosis are simpler and easier to guess than those of meiosis. Tell students that there will be more practice on these concepts, so they should not worry about memorizing specific details at this time. 3 Cell Division and Differentiation – Grade Ten Group time: Model Building 9. Have students retrieve their cell division posters and return to their groups. Ask them to quickly reorganize the concept maps of mitosis and meiosis based on the interactive lecture. 10. Use the corrected concept maps as a template, have each pair of students construct a model of mitosis and one of meiosis in an organism with a diploid number (2n) of four chromosomes. Provide model-building materials. Instructional Tip: Materials could include: pipe cleaners with a sticker for centromere, modeling clay of different colors, paper cutouts with a brad for a centromere, coins for centrioles, tissue paper for nuclear membrane, wax-coated string. 11. Instruct students to choose roles of reader or artist. They can alternate roles, if they desire. The reader will read the description and the notes on the first step depicted on the concept map as the artist creates it, then on to the next step, etc. Have each pair of students go through all the steps of mitosis and meiosis. Circulate around the room to keep student groups on track and provide direction. Instructional Tip: Encourage students to try to depict steps as accurately as possible and to make cell parts “move” as they go from one step to the next. Students should realize that in reality there is a continuous flow through the steps rather than a series of snapshots that suddenly change from one step to the next. Ask them to “re-enact” the video clip. 12. Assign each pair of students a separate step in mitosis or meiosis to depict for a classroom summary model. After the students have completed their models, attach them to one poster board, putting the model next to the index card describing each step. Display the poster with the models in the room. Direct Observation 13. Set up microscope stations with slides of various stages of mitosis and meiosis. Slides of white fish blastulae, ascarid eggs or allium root tips provide good examples. Arrange microscopes in order of events. 14. Direct students to look for various phases of cell division and draw what they see in their journals. Allow enough time for an observation and a quick sketch at each station. If microscopes and/or time are not available, use photographs of different stages of mitosis and meiosis set out at stations for students to view and sketch. Many Web sites are available with photo micrographs of cells undergoing mitosis and meiosis. 15. Set out samples or pictures of actual human karyotypes for students to observe. 4 Cell Division and Differentiation – Grade Ten Mitosis and Meiosis Wrap-up 16. Answer the following questions in journals: a. Note similarities and differences in the processes of mitosis and meiosis. b. Note similarities and differences in the results of mitosis and meiosis. c. Note locations and uses for each type of cell division in the body. After students have had some time to write independently, have them pair up and share ideas with their partners. They should add new answers or ideas in the journals. Sample responses can be found in Attachment A, Student Journal Requirements. Part One Closure Discussion 17. Conduct a class discussion of wrap-up questions (Step 14). Students should contribute to the discussion and should revise journals with added information as needed. Part Two: Cell Differentiation Cell-type Brainstorming 18. Have each group of four students make a list of types of cells in the body and what organelles or features might be especially important for each type (e.g., muscle cells have a higher number of mitochondria for energy than other cells and white blood cells have more lysosomes). Encourage students to think about the main function of the cell and what structures are needed to perform that function on a cellular level. After some time, ask for volunteers to share answers. List answers on flip chart or blackboard. Interactive Lecture 19. Relate the concept that specific genes are “turned on” or activated in different types of cells to enable that particular cell to be differentiated for a specific function. All cell types carry the full complement of genetic material (DNA), but only a select amount of genetic material will be expressed in a given cell. The genes that are translated into proteins will determine structures present in a particular cell and, therefore, the functions of that cell. Emphasize that structure relates to function in the cell. Exploring Embryogenesis 20. Divide students into four groups and have each student fill out a nametag with one of the following: endoderm (three to four students), ectoderm (three to four students) mesoderm (three to four students) and trophoblast (remaining students). 21. Explain that they are a zygote that has just undergone a series of mitotic divisions without gaining new cytoplasm. This “late cleavage” stage with its ball of small cells (stem cells) is now about to form an early embryo. 22. Ask students to review the number of chromosomes in each stage: gamete, zygote and now cells of the blastocyst. (Answers: n, 2n, 2n) 23. Direct the students as they represent the different types of cells present in the early embryo. Have “Trophoblast” students form a circle around the other three groups. Next have “Ectoderms” stand in a row, “Mesoderms” form a second row and “Endoderms” form a third row. 24. Ask a student from each group to recall word roots: ecto-, meso-, endo- and troph5 Cell Division and Differentiation – Grade Ten 25. Give clues as needed. (Answers: outside, middle, inner, nourish.) 26. Tell students what each will be able to become: Ectoderm—epithelium and nervous system; Mesoderm—connective tissue, muscles, bones, most organs; Endoderm—mucous membrane linings of digestive and respiratory tracts; Trophoblasts—part of the placenta (nourishment). 26. Compare these groups of cells (students) to actors at a casting call prepared to take on different roles, then trained specifically for their parts. Direct students in each group to choose a cell type to portray as you go through these lists. Instructional Tip: A person in the ectoderm group might become a brain cell, a skin cell, the lens of the eye, or a hair. Mesoderm can become a bone, blood, a vocal cord, a bicep muscle, a kidney or an appendix. Endoderm can line your nose, stomach or lung bronchioles. Trophoblasts will stay in their circle and lend support and nourishment to the developing embryo. Direct Observation 27. Set up stations around the room for the following three activities: Set up microscope stations with starfish development composite slides. Observe and sketch. Record observations and sketches in journal; Give students an unlabeled handout picturing the fertilized ovum, early and late cleavage, blastocyst with inner cell mass and trophoblast, implantation and early embryo showing three germ layers (ectoderm, mesoderm and endoderm). Ask students to label the diagram with help from textbook or other visual aids set out at stations. In the embryo picture, students should circle the layers they represented in the embryogenesis exploration and give examples of what type of cells they could become. Add this handout to journal. A third station has pictures or Websites featuring embryos of different species. Students sketch at least three different embryos in their journal. All students should rotate through all three stations. Have students record answers to the following questions in their journal: 1. What were some similarities and differences you saw between the embryos of the various species? 2. Which part of the embryo did you portray in the skit? What possible cell types could you have become? 3. Comparing the cells of the late cleavage stage with an ectoderm cell, which one do you think has a greater capacity for differentiation? Explain your answer. Sample responses can be found in Attachment A, Student Journal Requirements. 6 Cell Division and Differentiation – Grade Ten Conclusion Discussion 28. Lead a discussion to review the concepts of specialized cells differentiated for specific functions, arising from three embryonic germ layers and allowing for complex multicellular life. Connections should be made back to chromosomes, genes, and specific genetic expression in specific cells. What are embryonic stem cells in light of what we have just learned? “Pluripotent cell” is the current way of referring to the cells of the embryonic ectoderm, endoderm and mesoderm that have a potential to become many different types of cells. “Totipotential cells” are early developmental cells that can be duplicated and have the potential to become any type of cell. Student Journaling 29. Give students time to summarize cell differentiation in terms of how and when it occurs and why it is necessary in multicellular organisms. 30. Have the students address the following critical thinking questions to conclude the lesson: a. Why are the offspring produced by sexual reproduction both similar to and different from the parents? b. How do the processes of cell division and cell differentiation illustrate the interdependence of structure and function? Sample responses can be found in Attachment A, Student Journal Requirements. Differentiated Instructional Support: Instruction is differentiated according to learner needs, to help all learners either meet the intent of the specified indicator(s) or, if the indicator is already met, to advance beyond the specified indicator(s). Journaling may be more directed using written questions and providing a specific format for student entries. Provide a handout with steps of mitosis and meiosis for students to cut out, illustrate and put in order for instructional support and home practice. Have cell cycle tutorials from Internet educational sites. Students create a study guide with graphic organizers to reinforce key vocabulary and processes. Challenge students to research and review steps of cell division and come up with their own file cards with illustrations for concept mapping. Have students study and present the differentiation of a hemocytoblast into various blood cells in a variety of presentation modes including poster, presentation software, etc. Extensions: Research the arguments for and against stem-cell research. Review quotes from witnesses given to Congress in hearings on stem-cell research legislation. Explore questions such as: What diseases might be cured using stem-cell technology? What are some advantages and disadvantages to using adult stem cells vs. embryonic stem cells? 7 Cell Division and Differentiation – Grade Ten Are science and technology ahead of society’s preparation for new ideas? Write an essay, article or editorial based on your research. Make a collection of recent major newspaper and magazine articles regarding embryology and stem-cell research. Explore the ideas of surface area to volume ratios and cues for mitosis. Calculate cell surface areas and volumes. Relate these ideas to why cells are microscopic. Research current articles about cancer that focus on cell signals for mitosis to start or stop. What is different in cancer cells that causes them to undergo continuous mitosis? Invite a guest speaker from the American Cancer Society or other medical groups. Choreograph a mitosis or meiosis dance using classmates as chromatids/chromosomes. You be the “caller” or choreographer, depending on the style of the dance. Interdisciplinary Connections: English Language Arts Acquisition of Vocabulary Benchmark E: Apply knowledge of roots and affixes to determine the meanings of complex words and subject area vocabulary. Indicator 5: Use knowledge of Greek, Latin and Anglo-Saxon roots, prefixes and suffixes to understand complex words and new subject-area vocabulary (e.g., unknown words in science, mathematics and social studies). Materials and Resources: The inclusion of a specific resource in any lesson formulated by the Ohio Department of Education should not be interpreted as an endorsement of that particular resource, or any of its contents, by the Ohio Department of Education. The Ohio Department of Education does not endorse any particular resource. The Web addresses listed are for a given site’s main page, therefore, it may be necessary to search within that site to find the specific information required for a given lesson. Please note that information published on the Internet changes over time, therefore the links provided may no longer contain the specific information related to a given lesson. Teachers are advised to preview all sites before using them with students. For the teacher: Pipe cleaners, modeling clay of different colors, paper, coins, tissue paper, wax-coated string, index cards, slides or photos of mitosis and meiosis in white fish blastulae, ascarid eggs or allium root tips, photos of embryonic development. For the students: Journals, pipe cleaners, modeling clay of different colors, paper, coins, tissue paper, wax-coated string, index cards, slides or photos of mitosis and meiosis in white fish blastulae, ascarid eggs or allium root tips, photos of embryonic development. 8 Cell Division and Differentiation – Grade Ten Vocabulary: mitosis meiosis gonad gene chromosome chromatid centromere centriole nuclear membrane cytokinesis replication stem cell pluripotential cells totipotential cells cell differentiation embryo cell tissue diploid haploid tetrad crossing over ectoderm endoderm mesoderm trophoblast blastocyst gamete zygote Technology Connections: There are many cell-cycle tutorials available for student use on the Internet. Use Web sites for extension exercises. Research Connections: Marzano, R., Pickering, D., Pollock, J. Classroom Instruction that Works: Research-based Strategies for Increasing Student Achievement, Alexandria, Va: Association for Supervision and Curriculum Development. 2001. Identifying similarities and differences enhance students’ understanding of and ability to use knowledge. This process includes comparing, classifying, creating metaphors and creating analogies and may involve the following: 9 Cell Division and Differentiation – Grade Ten Presenting students with explicit guidance in identifying similarities and differences; Asking students to independently identify similarities and differences; Representing similarities and differences in graphic or symbolic form. Nonlinguistic representations help students think about and recall knowledge. They include the following: Creating graphic representations (organizers); Making physical models; Generating mental pictures; Drawing pictures and pictographs; Engaging in kinesthetic activity. Cooperative-learning groups have a powerful effect on student learning. This type of grouping includes the following elements: Positive interdependence; Face-to-face promotive interaction; Individual and group accountability; Interpersonal and small-group skills; Group processing. Setting objectives and providing feedback establish a direction for learning and a way to monitor progress. This provides focus on learning targets and specific information to allow the student to make needed adjustments during the learning process, resulting in increase student learning. Attachments: Attachment A, Student Journal Requirements 10 Cell Division and Differentiation – Grade Ten Attachment A Student Journal Requirements 1. 2. 3. 4. 5. 6. 7. 8. Pre-assessment questions and revised answers Sketches of mitosis and meiosis from microscope observation Human karyotype sketch Mitosis and meiosis wrap-up questions and revised answers Cell types and specializations list (at least five) Embryo sketches and observation questions Cell differentiation summary Answers to the following critical thinking questions: a. Why are the offspring produced by sexual reproduction both similar to and different from the parents? b. How do the processes of cell division and cell differentiation illustrate the interdependence of structure and function? 9. Any extension activities completed 11 Cell Division and Differentiation – Grade Ten Attachment A (continued) Student Journal Requirements Scoring Guidelines: Criteria Required elements Understanding: Accuracy Processes of mitosis and meiosis Level 4 All parts completed, including an extension. Similarities and differences are accurate, thoughtfully explained. Communication:Clarity, Information is Focus, Organization communicated clearly and precisely, effectively focused and organized and also may include inventive/expressive dimensions. Evidence of Inquiry: Analyses are Analysis and accurate. conclusions Conclusions are Embryogenesis valid, detailed and observations and consistent with data. questions, Level 3 All parts completed. Level 2 Level 1 At least six Fewer than six parts completed. parts completed. Similaritites and differences are accurate. Similarities and differences have occasional inaccuracies or are simplified. Information is communicated clearly, and is focused and organized. Information has some clarity, focus and organization. Analyses are accurate. Conclusions are valid and consistent with data. Analyses are mostly accurate. Conclusions are related to the data. Analyses are unclear or inaccurate. Conclusions are unclear or unrelated to the data. Applications to personal and societal issues are identified. Applications to personal and societal issues are suggested or implied. Applications are unclear or absent. Critical-thinking questions Relevance to Society: Person and society Cell differentiation summary, Critical-thinking questions Relevant applications to personal and societal issues are identified and insightfully described. Similarities and differences have major inaccuracies or are overly simplified. Information is unclear, lacks focus and organization. Adapted from Council of Chief State School Officers State Collaborative on Assessment and Student Standards (SCASS) Science Project, April 1997. 12 Cell Division and Differentiation – Grade Ten Attachment A (continued) Student Journal Requirements Examples of acceptable student responses: Pre-assessment Questions: 1. Answers will vary from a simple X or a wavy line to an attempt at a double helix structure, DNA. Students should circle a small area or areas along the chromosome they have drawn to indicate the location of a gene. 2. The process of mitosis makes an exact copy of the DNA for each daughter cell. Lost surface cells are constantly being replaced by new cells being produced my mitosis in a deeper cell layer. 3. The process of meiosis results in a sperm cell with one copy of each gene from the father to be included in the fertilized egg. 4. Every cell holds a complete copy of an individual’s DNA. Cell differentiation occurs throughout embryonic and fetal development as stem cells become various cell types depending on which portions of their DNA are expressed. Mitosis and Meiosis Wrap-up Questions: a. Note similarities and differences in the processes of mitosis and meiosis. Sample Answer: a. Mitosis involves only one nuclear division, whereas in meiosis the nucleus undergoes two divisions. In mitosis, chromosomes are copied and distributed identically to daughter cells, whereas in meiosis, chromosomes undergo crossing over and redistribution of genes to gametes. b. Note similarities and differences in the results of mitosis and meiosis. Sample Answer: Mitosis results in identical daughter cells, two, each containing the diploid (2n) number of chromosomes. Meiosis results in gametes containing the haploid (n) number of chromosomes. Each of four resulting gametes will differ from one another genetically. c. Note locations and uses for each type of cell division in the body. Sample Answer: Mitosis occurs in all cells of the body with the exception of the gonads. Meiosis takes place in the ovary and testis and produces the gametes for sexual reproduction. Cell types and specializations: White blood cells: lysosomes; Muscle cells: mitochondria; Skin cells: flat sheets, melanin pigment; Red blood cells: no nucleus, full of hemoglobin protein; Nerve cells: cell extensions (axons), rough endoplasmic reticulum; Intestinal lining cells: microvilli for absorption, tight junctions between cells; Gland cells: secretory granules, rough endoplasmic reticulum, hormone receptors; Sensory receptor cells: stereocilia, chemical receptors. 13 Cell Division and Differentiation – Grade Ten Attachment A (continued) Student Journal Requirements Questions about the embryo observations: 1. What were some similarities and differences you saw between the embryos of the various species? Sample Answer: There are differences in size, shape, tail length, limb development. The similarities lie in the shape of the embryos, especially in the early stages. 2. Which part of the embryo did you portray in the skit? What possible cell types could you have become? Sample Answer: Ectoderm: brain cell, a skin cell, the lens of the eye, or a hair. Mesoderm: bone, blood, subcutaneous fat, vocal cord, muscle, heart, kidney or appendix. Endoderm: inside of mouth and nose, stomach lining or lung air sacs. Trophoblast: support and nourishment for developing embryo. 3. Comparing the cells of the late-cleavage stage with an ectoderm cell, which one do you think has a greater capacity for differentiation? Explain your answer. Sample Answer: Late-cleavage cells have greater capacity for differentiation because they can become all of the different germ layers and, ultimately, every type of body cell. Once in the embryo stage, the different germ layer cells can differentiate, but only within limits. Cell Differentiation Summary: Summarize cell differentiation in terms of how and when it occurs and why it is necessary in multicellular organisms. Answer should include the following points: Specializations are needed for cells to perform specialized functions; Specialized cells are needed for division of labor in multicellular organisms; Structure of specific cell types reflects their functions; Specializations are made possible because all cells contain the full complement of DNA but not all DNA is expressed in every cell. Certain genes are activated in specific cells; Differentiation of the major germ layers occurs as the embryo is formed. As the embryo develops further these germ layer cells (eg. Ectoderm) further differentiate into specific cell types found in the fully developed fetus. Critical Analysis Questions: a. Why are the offspring produced by sexual reproduction both similar to and different from the parents? During the process of meiosis, which results in gametes (egg or sperm cells), chromosomes from the father or mothers’ parents are randomly arranged and divided so that alleles (sections of DNA encoding specific proteins) from both the grandfather and grandmother will be present for various traits in each gamete formed. Most alleles are 14 Cell Division and Differentiation – Grade Ten Attachment A (continued) Student Journal Requirements passed on independently from one another (independent assortment), although many appear to be connected and will be passed on together. The randomness of which alleles will be received by a given egg or sperm cell is increased due to a process during meiosis called “crossing over,” where parts of homologous chromosomes from the grandparents are exchanged before chromosomes divide and move into their respective gametes. Each gamete (egg or sperm) contains genes from both grandparents with only half the normal number of chromosomes. When egg and sperm unite, the genetic material unites as well and a complete set of chromosomes results in the fertilized egg. Both parents contribute to the DNA of the offspring, and, in turn, the genes of the grandparents also are present. The assortment of combinations of the traits from previous generations allows for offspring to be different from either parent, yet similar at the same time. b. How do the processes of cell division and cell differentiation illustrate the interdependence of structure and function? Cell division can occur only because specific structures such as centrioles, spindle fibers and DNA polymerase are present in the cell. Each of these structures is specifically designed to carry out its specific function. Without any part of the cell machinery present, cell division could not occur. As a result of cell differentiation, one sees the various organelles and structural proteins in the cell or tissue that determine the function of that cell or tissue. Examples include mitochondria for making ATP in skeletal muscle, lysosomes in white blood cells that engage in phagocytosis and collagen fibers produced by connective tissue cells that impart strength to skin, cartilage and bone. Each cell and tissue type is uniquely fit to carry out its given functions because of the structures it possesses. 15