INSTRUCTOR:
Dr. Raymond E. Menard
Office: SC 120
Phone: 341-3561
Email: menard.ray@spcollege.edu
Office Hours: Monday and Wednesday: 3:30-4:30pm
Tutoring: Thursday 12:45-1:45pm SC 213
ACADEMIC DEPARTMENT: Department of Natural Science and Wellness
Mr. John Chapin/ Dean
Office: SE LI 281
Phone: 394-6995
Department of Natural Science and Wellness
Dr. Bev Grundset/ Academic Chair
Office: SC 133
Phone: 341-4343
COURSE PREFIX AND NUMBER: MCB 2010L
COURSE NAME: Microbiology Lab
This laboratory will consist of experiments that teach bacterial techniques, characteristics of
morphology, growth responses, metabolic activity, and distribution in selected environments.
MEETING INFORMATION:
Class meets in SC 213
Tuesday and Thursday from 11:00am to 12:15pm
ATTENDANCE: Attending class and being an active participant are crucial to your success in
this class. The majority of the exam material will come from notes presented in class, so regular
attendance should improve your exam scores. Attendance will be taken every class period and
students who miss are responsible for all work due that day as well as any material presented in
class that day. If you must miss class due to a serious illness or other event, please let me know
so that I am aware of the situation
SPECIAL ACCOMMODATIONS:
If you wish to request accommodations as a student with a documented disability, please make
an appointment with the Learning Specialist on campus. If you have a documented hearing loss,
please contact the Program for the Deaf/Hard of Hearing at 791-2628. If you need assistance
during an emergency classroom evacuation, please contact your campus learning specialist
immediately about arrangements for your safety. The Office of Services for Students with
Disabilities can be reached at 791-2628 or 791-2710 (CL), 341-4758 (SP/G), 394-6108 (SE)
712-5789 (TS) or 341-4532 (AC).
COURSE GOALS:
1.
The student will develop the skills and techniques of microbiology that provide an understanding
of microorganisms and methods of identification.
2 The student will accurately perform and interpret scientific tests to gain confidence and skill in
problem solving techniques.
COURSE OBJECTIVES:
1. The student will develop the skills and techniques of microbiology that provide an
understanding of microorganisms and methods of identification by:
a. demonstrating proper aseptic techniques.
b. using appropriate microbiological media and test systems.
c. using standard microbiology laboratory equipment correctly.
d. accurately recording and interpreting laboratory data.
e. demonstrating and explaining safe laboratory practices for handling biohazardous
materials.
f. identifying microorganisms by growth habits, nutritional requirements, staining reactions, and
biochemical reaction.
g. evaluating different anti-microbial agents as control mechanisms of microorganisms.
h. demonstrating appropriate care and use of the microscope
2. The student will accurately perform and interpret scientific tests to gain confidence and skill in
problem solving techniques by:
a. formulating clear, answerable questions, developing testable hypotheses, predicting
expected results, and following experimental protocols.
b. collecting and organizing data in a systematic fashion, assessing the validity of the data, and
drawing appropriate conclusions based on the results.
c. working effectively in groups or teams so that the task, results, and analysis are shared.
d. effectively managing time and tasks allowing concurrent and/or overlapping tasks to be
done simultaneously, by individuals and within a group.
e. integrating knowledge and making informed judgments about microbiology in everyday
life.
PREREQUISITES :
Corequisite: MCB2010
GRADING:
Grading System:
3 Lab Exams (100pts)
Quizzes
Lab Reports
Unknown Lab Report
300
75
75
50
Grading Scale:
90-100% = A (450-500)
80-89% = B (400-449)
70-79% = C (350-399)
60-69% = D (300-349)
Below 60% = F (<300)
Total Points: 500
Lab Exams:
The lab exams will primarily focus on material presented in lab with some reading
material included from your lab book. The exams will consist of a combination of multiple
choice, fill in the blank, short answer, and essay questions.
Lab Reports:
Lab reports are assigned throughout the semester to help you understand the experiments
better. Refer to the schedule for the due dates on these reports. Reports are to be
uploaded in the drop box set up in mycourses by 11:30pm on the
day they are due. Once the drop box closes you will not be able to
submit your report and therefore will receive a zero.
Unknown lab Report:
You will be given an unknown bacteria to identify towards the end of the semester. You will use
techniques learned in class to identify this unknown. You will then submit a comprehensive lab
report on this specimen. Again this report needs to be submitted to the drop box that has been set
up.
QUIZZES: There will be 5 quizzes throughout the semester that you will need to take on line.
The due dates to take the quizzes are listed in the schedule at the end of this document. The
quizzes will have 15 questions and you will have 45 minutes to take the quiz. You will have one
attempt so be sure you check all of your answers before you submit it.
Make-up Exams:
Any student missing a lab exam may take a make-up provided they have a legitimate
excuse for missing class (i.e. severe illness with doctor’s note, family emergency). All scores
on make-up exams will be reduced by 10% each time you need to take one. This penalty
applies to ANYONE taking a make-up, No Exceptions! The 10% penalty is additive, so the
first make-up is -10%, the 2nd -20%, the 3rd -30% etc… Make-up exams must be taken within
one week of the original test date and note that make-up exams may be of a different format than
the original.
Lab Attire: In this lab we are working with MICRO-ORGANISMS. Therefore it is important
that you wear the proper clothes. NO OPEN TOED SHOES….you do not want to spill
any cultures on your skin. We will discuss safety during the first lab period. Also, if you
have long hair, please have it pulled back, we will use Bunsen burners in this class and
you don’t want to set your hair on fire.
You need to wear a lab coat. Please put your name on the lab coat and bring a bag to
store it in.
Grades Changes: Please follow the guidelines listed below if you feel that an answer on an
exam, quiz, homework, or other assignment has been erroneously marked incorrect. I will either
post a key or go over questions/answers in class with you for any assignments/quizzes/exams
that are given.
1) Please circle the answer in question and return to me within one week from the day
you receive the assignment/exam back.
2) I will review the appropriate answers and determine if your grade needs to be
adjusted. I will return your assignment/exam back to you within one week from the
time you give it to me.
3) The time frame therefore for changing a grade on any given assignment is TWO
WEEKS. If for some reason you are ill or otherwise engaged, you may make an
appointment with me, but the appointment needs to be made within the two week
time frame.
Please do not ask me during class time to review your papers for a grade change as there is no
time for me to assess the situation while in class.
Classroom Behavior:
Success in this and any college class requires good note taking, test taking , and study
skills. Please ask me for assistance with any of these areas. Do not wait until mid-term before
trying to improve your work. Start asking questions and looking for help early on if you need it.
In addition, attending class means more than just showing up. Come prepared to take notes, ask
questions, and listen. Please be considerate of other students as well as myself during lecture. It
is distracting to hear someone talking or laughing during lecture so please refrain from side
conversations during lecture. Students who continue to cause disruptions in class will be
asked to leave the classroom. For more detailed information on proper student conduct please
see the code of conduct in your course catalog.
Please turn off all beepers and cell phones while in class. Cell phones going off during class
are a distraction during lecture and exams. You will be given one warning if your cell phone
goes off during class before being asked to leave class for that period. If you need to use your
phone please do so outside class even if this is during a break time.
Finally, coming late to class is distracting to me and, more importantly, your fellow
students so please be on time for all class meetings. In addition, missing the first 10 minutes
of class means you will miss important announcements about the schedule, assignments, quizzes,
etc. If you do arrive late please enter the class and take your seat quietly. Do not ask students or
myself questions at this time or copy notes that you missed. You can ask questions or copy notes
after class.
Anyone arriving more than 10 minutes late for an exam will be asked to leave and will have to
take the exam late with the 10% penalty as per the make-up exam policy. For more detailed
information on proper student conduct please see the code of conduct in your course
catalog.
ACADEMIC HONESTY:
St. Petersburg College has an academic honesty policy. It is your responsibility to be
familiar with the policies, rules and consequences of violations. Read about the policy at:
http://www.spcollege.edu/webcentral/admit/honesty/htm. There is no tolerance for cheating
and academic dishonesty. The penalty can range from a zero on that assignment or exam to
a grade of F in the class.
MCB 2010L Microbiology Laboratory--
Tentative Schedule Fall 2015
St. Petersburg/Gibbs Campus
Lab Manual and Power point Picture atlas in MyCourses
Lab
1
2
TuTr
8/18
8/20
3
8/25
4
5
8/27
9/1
6
9/3
7
8
9/8
9/10
9/15
Topic
Introduction to the lab; Requirements; Lab safety
Ubiquity of Microorganisms
Hand washing Lab
Aseptic Techniques; Streak Plate
Observe Ubiquity Results and Hand washing Results
Observe streak Plate
Basic bacterial morphology.
Use of the microscope & oil immersion.
LAB REPORT 1 DUE: Ubiquity and Hand washing due in
drop box by 11:30pm
Eukaryotes: Molds and Yeast
Quiz 1: DUE Friday Sept 4 by 11:30pm
Continue Microscopy and Exam Review
EXAM 1
Simple Stain/ Make smear unknown
Lab book pg#
5-6
22
24
26-31
23, 25
31
7-11
12
13-15
9
10
9/17
9/22
11
9/24
12
9/29
13
10/1
14
10/6
15
10/8
16
17
18
10/13
10/15
10/20
10/22
19
10/27
20
10/29
21
Gram stains on gum smears. KOH method of Gram analysis
Read Gram Stains / Demo Slides: Spore Stain, Flagella,
Capsular, and Acid Fast
Effect of pH and Osmotic pressure on microbial growth
Quiz 2: Due Friday Sept 25 by 11:30pm
Aerotolerance/Temp Tubes
Observe pH/Osmotic Tubes
Antibiotic susceptibility
Observe Aerotolerance/Temp Tubes
pGlo Genetic Transformation of E. coli
Observe Antibiotic sensitivity plates
Observe pGlo Plates
Exam Review
Quiz 3: Due Friday Oct 9 by 11:30pm
EXAM 2
Simulated HIV/ELISA TEST
All College Day- No Labs
Staphylococcus Identification Lab
Quiz 4: Due Friday Oct 23 by 11:30pm
PEA/EMB
Inoculate PR and MRVP fermentation Broths, Urease, PAD
Observe Staph Identification results
Observe PR fermentation/Urease/PAD/PEA/EMB
LAB REPORT 2: Staph Identification due in drop box by
11:30pm
16-19
20-21
11/3
Set Up Citrate, SIM, Catalase, Oxidase
54-55
22
11/5
UV Radiation
Observe Citrate, MRVP, SIM
42
53,55
23
11/10
Discuss Unknown Project
Observe UV radiation results
64-66
43
24
11/12
25
26
27
11/17
11/19
11/24
11/26
12/1
12/3
Begin Unknowns
Lab Report 3 Due: UV light Due in dropbox by 11:30pm
QUIZ 5: Due Friday Nov 13 by 11:30pm
Unknowns continued
Unknowns continued
Unknowns continued
Thanksgiving Break – No Lab
Clean up Exam Review
Final Lab Exam
UNKNOWN LAB REPORT DUE in dropbox by 11:30pm
28
29
Last day to drop, get refund or change to audit 8/21/15
Last day to withdraw with a “W” 10/22/15
32-33
34-37
34
40-41
38-39
56-62
41
63-64
66-68
45-48
49
51-52
48
53