MARE 394 – Dogfish Dissection #1 – External Anatomy, Musculature, & Digestive System You will be responsible for all systems listed in the lab whether there is a specific figure to represent each in your dissection guide. You are asked to draw each of the tissues as they appear in your animal - not as they appear in the dissection guide. 1. External Anatomy Orient yourself to the shark by identifying the following external features: a. Lateral line b. Anterior dorsal fin c. Posterior dorsal fin d. Pectoral fin e. Caudal fin f. Pelvic fin g. Spiracle h. Lateral line i. Eye j. Gill slits k. Nostrils l. Cloacal opening m. Ampullae of Lorenzini n. Clasper (♂) o. Endolymphatic pore 2. Musculature Using a scalpel and forceps, carefully remove the skin covering the muscles illustrated below from the mouth to the anterior dorsal fin that appear in Fig. 5 (p.10). Identify the following features: 3. Dorsal longitudinal bundles 4. Dorsal superficial constrictor 6. Extensor of fin 10. Lateral longitudinal bundle 13. Quadratomandibularis 17. Ventral longitudinal bundle 3. Digestive and Respiratory Systems Using scissors, make a cut from the corner of the jaw through the five gill arches. Just posterior to the fifth gill arch, make a cut across the throat just between the heart (anterior) and the abdominal cavity (posterior). Identify the following features from the mouth and pharynx in Fig 8 (p.15). 3. Esophagus 9. Gill rakers 17. Pharynx 18. Spiracle 20. Tongue Using the scalpel, make a shallow incision in the middle of the abdominal cavity halfway between the cloaca and the pharyngeal cavity. Once the incision has been made, carefully insert forceps to pull the abdominal wall away from the internal organs so that the incision can be extended to the pharynx (anteriorly). Cut transversely across the abdominal wall at the pharynx and just above the cloacal opening so that the wall sections can be folded open to expose the internal organs. Pin the abdominal walls to the dissection pad to hold them in place; you may need to score the internal abdominal wall to relieve the tension. Identify the following features of the digestive system from Fig. 11 (p.19). 3. Bile duct 4. Body of stomach 5. Cardiac region of stomach 9. Colon 13. Dorsal lobe of pancreas 14. Duodenum 15. Falciform ligament 16. Gall bladder 22. Left lobe of liver 25. Median lobe of liver 33. Pyloric region of stomach 34. Rectal gland 35. Right lobe of liver 37. Spleen 39. Valvular intestine 41. Ventral lobe of pancreas Using scissors or scalpel, cut open portions of the esophagus to expose the papillae, the stomach to expose the rugae, and the intestine to expose the spiral valve following the figure on page 22. Examine the gut contents of the stomach and compare with other students.