MARE 394 – Dissection #5 – Ratfish You will be responsible for all systems listed in the lab whether there is a specific figure to represent each in your dissection guide. You are asked to draw each of the tissues as they appear in your animal not as they appear in the dissection guide. 1. External Anatomy Orient yourself to the ratfish by identifying the following external features: a. Lateral line b. Anterior dorsal fin c. Posterior dorsal fin d. Pectoral fin e. Caudal fin f. Pelvic fin g. Spiracle h. Lateral line i. Eye j. Gill slits k. Nostrils l. Cloacal opening m. Ampullae of Lorenzini n. Clasper (♂) o. Endolymphatic pore 2. Digestive and Respiratory Systems Using scissors, make a cut from the corner of the jaw through the five gill arches. Just posterior to the fifth gill arch, make a cut across the throat just between the heart (anterior) and the abdominal cavity (posterior). Identify the following features from the mouth and pharynx in Fig 8 (p.15). 3. Esophagus 9. Gill rakers 17. Pharynx 18. Spiracle 20. Tongue Using the scalpel, make a shallow incision in the middle of the abdominal cavity halfway between the cloaca and the pharyngeal cavity. Once the incision has been made, carefully insert forceps to pull the abdominal wall away from the internal organs so that the incision can be extended to the pharynx (anteriorly). Cut transversely across the abdominal wall at the pharynx and just above the cloacal opening so that the wall sections can be folded open to expose the internal organs. Pin the abdominal walls to the dissection pad to hold them in place; you may need to score the internal abdominal wall to relieve the tension. Identify the following features of the digestive system from Fig. 11 (p.19). 3. Bile duct 4. Body of stomach 5. Cardiac region of stomach 9. Colon 13. Dorsal lobe of pancreas 14. Duodenum 15. Falciform ligament 16. Gall bladder 22. Left lobe of liver 25. Median lobe of liver 33. Pyloric region of stomach 34. Rectal gland 35. Right lobe of liver 37. Spleen 39. Valvular intestine 41. Ventral lobe of pancreas Using scissors or scalpel, cut open portions of the esophagus to expose the papillae, the stomach to expose the rugae, and the intestine to expose the spiral valve following the figure on page 22. Examine the gut contents of the stomach and compare with other students. 3. Heart Use the schematic on page 23, Fig 13 (p. 24) and Fig 17 (p. 29) to identify the following structures of the heart. Conus arteriosus Atrium Ventricle Sinus venosus Dorsal aorta Coronary artery Afferent branchial arteries Efferent branchial arteries Celiac artery Ventral aorta 4. Urogenital system Use figure 19 (p. 33) to identify the following features of the ♂ urogenital system. 4. Clasper 5. Cloaca 6. Colon 8. Ductus deferens 9. Epididymis 10. Kidney 12. Left testis 13. Leydig’s gland 16. Opening siphon into clasper tube 21. Right testis 22. Seminal vesicle 23. Siphon 24. Sperm sac 25. Urogenital papilla IF WE HAVE FEMALE RATFISH Use figure 20 (p. 35) to identify the following features of the ♀ urogenital system 4. Cloaca 5. Colon 9. Embryo with uterus 12. Kidney 13. Left oviduct 16. Opening of uterus into cloaca 5. Brain Cerebellum Cerebrum Optic lobe Olfactory Lobe Olfactory Bulb 17. Ostium to oviducts 18. Ovary containing eggs 20. Oviduct 23. Urogenital papilla 24. Uterus containing embryos 26. Yolk sac