DISSECTION INFORMATION GUIDE *Please read this guide. The material on pages 1, 2, and 6 are included on the Muscular System Exam.* Cats will be distributed on Tuesday, February 16th. One student from each dissection group should report to Mr. Specks during their off period. I will provide a specific location. Your group will be assigned a cat/dissection kit and you will be briefed on general dissection instructions. Please know your Group # so that you will be assigned the correct materials. Cat dissection will officially begin Thursday, February 18th. Your cat needs to be skinned by the start of class on the 18th. If done correctly and instructions have been read beforehand, skinning should take approximately one hour. You are welcome to do this in your off period, lunch period, before, or after school once the cats have been assigned. Mr. Specks will instruct you to the rooms you may use. NO LIVE ANIMALS ARE TO BE PRESENT. DO NOT ENTER THE SURGICAL AREA UNDER ANY CIRCUMSTANCES. Please use the information in this guide, the dissection powerpoint, as well as your notes from class and the book to aid you in your studies and dissection of the cat. The labs will take place in the basement, during your regularly scheduled A&P class time. Your group will be assigned to a specific basement classroom. The instructions for each day are listed in this guide. If all of the day’s tasks have been completed, continue on to the instructions for the following day. Arriving late/leaving early is not allowed. An absence will result in a 5 point deduction from your Dissection Exam grade. USE YOUR TIME WISELY! STUDY HARD! HAVE FUN! We will dissect for 4 days: Thursday, February 18th to Tuesday, February 23rd. The Dissection Exam will take place on Wednesday, February 24th. You will be tested on the following about the muscles and structures listed in this guide: location, origin, insertion, and function. *THESE CATS HAVE BEEN PREPARED FOR YOUR EDUCATIONAL PURPOSES. YOU MUST TREAT THEM WITH RESPECT AND CONDUCT YOURSELVES PROFESSIONALLY IN THE LAB. ANY STUDENT BEHAVING INAPPROPRIATELY WILL BE ASKED TO LEAVE AND HAVE PROFESSIONALISM POINTS DEDUCTED.* EVERY DAY GUIDELINES: 1. Pull your hair back from your face and remove any jewelry that will tear your gloves or dangle into the dissection field. 2. Wear your face mask! Don’t lose it. It will be used during the entire dissection week. 3. Each group should have one dissection tray, one dissection kit with a blade, and one dissection guide book. You must use the materials assigned to your group. 4. Glove up! You will have one pair of gloves for each day so be careful. Notify instructor if your gloves tear. 5. Remove your cat from the bag and place it on the dissection tray. PLEASE KEEP THE PRESERVATIVE FLUIDS THAT COME WITH THE CAT IN THE BAG! THESE WILL HELP KEEP THE CAT MOIST FOR THE NEXT WEEK. 6. You must clean up your work station and supplies daily. Do not allow any tissue to enter the sink/tub when rinsing your tray. Be sure to store your cat so that the fluids do not leak out of the bag. 7. You are welcome to bring pins to identify and label the cat as you dissect it. Paper/tape directly on the cat does not stay on. 8. You are welcome to take pictures/videos to help in your learning as long as they are not misused or placed on the internet. REMOVING THE SKIN 1. This must be completed before the start of dissection. Read the instructions beforehand to lessen the time spent on skinning. 2. Carefully place the scalpel blade on the handle if there is not an attached blade in your kit. 3. Use the scalpel or a pair of scissors to start a longitudinal incision on the ventral midline at the neck. Be careful not to cut into the muscle or body cavities. There should already be a precut area in the neck. Begin there. 4. Extend the incision caudally to the inguinal region between the legs. Avoid cutting the genitals. 5. Make horizontal incisions along both thoracic and pelvic limbs as shown and cut skin around all 4 paws. 6. Cut around the base of the tail, leaving the skin on the tail. Cut the skin around the face of the cat, leaving the skin on the ears, eyes, and forehead. Remove the skin over the cheeks. 7. Peel the skin away from the underlying musculature using your fingers. The skin should only be left around the face, ears, forehead, and tail and should be removed in one piece. 8. Remove as much of the subcutaneous fat and as possible. This will save time when you start the actual dissection. You should be able to see muscles after the skin is removed. The cutaneous trunci muscle will probably be removed with the skin due to its location. You do not need to separate it from the skin. 9. Wrap the skin around the cat and store your cat in its plastic bag with the preservative fluids. The skin will prevent the tissues from drying out and prevent the growth of bacteria and mold. Dispose of any removed fascia and fat in a trash can, NEVER in the sink. 10. Do not begin dissecting the muscles until we start class dissection. 11. Do not open the body cavities of the cat. We will do this at the end of the week if time permits. 12. Identify the gender of your cat. DAY 1 ACTIVITIES IDENTIFY THE FOLLOWING MUSCLE OF THE SKIN Cutaneous trunci – thin, broad muscle in the fascia just under the skin; FUNCTION: twitch the skin IDENTIFY THE FOLLOWING MUSCLES OF THE HEAD AND NECK Masseter- large, most powerful muscle of mastication; FUNCTION: close the jaw Sternomastoid –straplike muscle on the ventrolateral surface of the neck; FUNCTION: turn the head Sternohyoid-straplike muscle on the ventral surface of the neck; FUNCTION: pull the hyoid dorsally IDENTIFY THE MUSCLES OF THE CHEST Pectorals- these chest muscles include the pectoantebrachialis, pectoralis major, pectoralis minor, and xiphihumeralis. Identify all 4 muscles of the pectorals. FUNCTION: adduct the front leg. IDENTIFY THE FOLLOWING MUSCLES OF THE ABDOMEN *FUNCTIONS: support the abdominal organs, flex (arch) the back, and participate in functions that involve straining (urination, defecation, vomiting, parturition)* External Abdominal Oblique – most superficial of the abdominal muscles; fibers run caudoventral Internal Abdominal Oblique – these fibers run cranioventral Rectus Abdominis – broad band of muscle on either side of the linea alba that forms the floor of the abdomen from the sternum to the pubis. These fibers run in a craniocaudal direction. Transversus Abdominis- deepest of the abdominal muscles; These fibers run in a transverse direction. Linea Alba – white aponeuroses down midline of abdomen that serves as the attachment site for abdominal muscles MUSCLE ORIGIN INSERTION Masseter External abdominal oblique Internal abdominal oblique Rectus abdominis Zygomatic arch Ribs and fascia Ilium and fascia First and second ribs Mandible Ventral midline Ventral midline Pubis DAY 2 ACTIVITIES IDENTIFY THE FOLLOWING SUPERFICIAL MUSCLES OF THE BACK & SHOULDER Trapezius – lies on the dorsal aspect of the neck; FUNCTION: extend (raise) the head and neck. It actually consists of 3 separate muscles - the clavotrapezius, acromiotrapezius, and spinotrapezius. Identify all 3 parts of the trapezius. Latissimus dorsi –broad triangular muscle that extends from the spinal column to the humerus; FUNCTION: Pulls front leg backwards (caudo-dorsal direction) Deltoids – These muscles of the shoulder consist of 3 separate muscles: the clavodeltoid, acromiodeltoid, and the spinodeltoid); Identify all 3 muscles of the deltoids; FUNCTION: abduct and flex the shoulder joint. NOTE: Some texts refer to the clavodeltoid as the clavobrachialis. This muscle extends from the clavicle to the radius and ulna. In addition to the above functions, it assists the biceps by acting as a synergist, as it aids in forearm flexion. IDENTIFY THE FOLLOWING DEEP MUSCLES OF THE BACK & SHOULDER Supraspinatus- fills the supraspinous fossa of the scapula; FUNCTION: extend the shoulder and stabilize the joint Infraspinatus – fills the infraspinous fossa of the scapula; FUNCTION: flex the shoulder and stabilize the joint *locate the spine of the scapula IDENTIFY THE FOLLOWING MUSCLES OF THE BRACHIUM Clavobrachialis- see note above Brachialis – extends from the lateral surface of the humerus to the proximal end of the ulna; FUNCTION: flex the elbow Biceps brachii – consists of 2 heads that extend from the scapula to the proximal radius; FUNCTION: flex the elbow Triceps brachii – 3 heads (long head of the triceps, lateral head of the triceps, and medial head of the triceps) that extend from the scapula and humerus to the olecranon process of the ulna; FUNCTION: extend (straighten) the elbow joint. Identify all 3 parts of the triceps MUSCLE Latissimus dorsi Pectorals Deltoids (acromio/spino) Brachialis Biceps brachii Triceps brachii ORIGIN lumbar vertebrae Sternum Scapula Lateral humerus Scapula Scapula and Humerus INSERTION Humerus Humerus Humerus Proximal ulna Radius Olecranon process of ulna DAY 3 ACTIVITIES IDENTIFY THE FOLLOWING MUSCLES OF THE PELVIC LIMB MUSCLES OF THE THIGH Gluteal muscles – this group consists of 2 separate muscles (the gluteus medius and gluteus maximus) that extend from the pelvis down to the femur; FUNCTION: abduct thigh. Hamstring muscles - this group consists of 3 separate muscles (the semimembranosus, semitendinosus, and biceps femoris) on the caudal aspect of the thigh: FUNCTIONS: extend the hip joint; flexes the stifle joint Biceps femoris-most lateral Semimembranosus-most medial Semitendinosus-most caudal Quadriceps femoris – this group consists of 4 separate muscles (rectus femoris, vastus lateralis, vastus medialis, vastus intermedius-not found in the cat) and is located on the cranial aspect of the thigh. The patella is located in the tendon of this muscle. Identify all 4 muscles of the quadriceps. FUNCTION: extend the stifle joint. MUSCLES OF THE LOWER LIMB Gastrocnemius muscle- this is the calf muscle; FUNCTION: extend the hock -Achilles tendon – large strong tendon of the gastrocnemius that runs down the back of the leg to attach on the calcaneal tuberosity IDENTIFY THE MUSLCES OF RESPIRATION Diaphragm – this muscle separates the thoracic cavity from the abdominal cavity. FUNCTION: assist in inspiration External intercostals – most superficial muscles between the ribs; FUNCTION: assist in inspiration Internal intercostals- lies deep to the external intercostals; FUNCTION: assist in expiration. MUSCLE Gluteal muscles Biceps femoris Semimembranosus Semitendinosus Quadriceps femoris Gastrocnemius ORIGIN Ilium and associated fascia Ischium Ischium Ischium Illium and femur Condyles of the femur INSERTION Greater trochanter of femur Tibia and patella Tibia Tibia Tibial tuberosity Calcaneal tuberosity of fibular tarsal bone DAY 4 ACTIVITIES This is the final day to review each muscle on your cat. When you feel that you are confident with the information, you may move forward and explore the thoracic and abdominal cavities, keeping the muscles of the chest and abdomen intact. After class, the dissection practical set-up will begin. The classroom will be LOCKED until the start of the exam on Wednesday, February 24th. For the exam, you will be expected to know the name, location, and function of ALL of the muscles that we have studied during dissection. The muscles listed in the charts are the only ones that will be tested on for origin and insertion. The exam will be worth 100 points. You will rotate to different stations around the room during the exam. You will be allowed one minute per station. The exam will be administered twice during each A & P class period. You will be assigned a testing time. NO SWITCHING OF TIMES WILL BE ALLOWED.