Stevia: Functional Food used as a Low Calorie Alternative Sweetener Research Paper Emily Brantley February 24, 2011 FOS 4041 Professor Ross Introduction In the past decade there has been an outburst of research and production of foods created with Stevia increased development of consumer food products that are sweetened with Stevia. Stevia is used as a functional food to sweeten a diverse variety of food consumer products. Some of these products include fruit juices, soda beverages, yogurts, ice creams, cereals and baked goods. Understanding the advantages and disadvantages of Stevia is significant because excessive sugar intakes have become a major health concern here in America. Approximately two thirds of American adults are currently overweight or obese. This means that the majority of our society is at risk for a high number of precarious health conditions with the two most severe being Type 2 Diabetes Mellitus and heart disease (1). The major culprit along with solid fats is a surplus of sugar, which on average provides 35 percent, or nearly 800 calories per person, per day (2). These disproportionate amounts create elevated levels of at risk members in our society. Staggering statistics like this have jumpstarted a Stevia phenomenon. There is a fundamental need for an alternative sweetener in place of sugar, or other chemical sweeteners. Stevia is getting more recognition than ever before as a functional food and a natural alternative to sugar to decrease or avoid these health risks altogether. The Stevia Rebaudiana (Bertoni) plant produces hydrophilic, sweet glycosides derived from its leaves. This shrub like plant belongs to the Chrysanthemum Family and it is native to South America in Paraguay and parts of Brazil. Although it has existed in this region for hundreds of years, the 2 biosynthesis of its steviol glycosides has only been researched and published within the last forty years (3). One of the notable advantages of Stevia extract is its powerful sweet flavor. In fact, only a small amount is necessary for the purpose of sweetening because it is 250 to 300 times sweeter than sucrose. Another favorable factor is its low calorie nature (4 & 5). This is beneficial for people who have excess sugar in their diet or for those who merely wish to cut back on sugar consumption. Stevia, when used as a functional food in place of sugar, can be used without the usual restrictions and recommended to the diabetic population especially since it is scientifically proven as ineffective to a person’s glucose levels (6). By examining the chemical composition, health benefits, flavor, absorption rate and marketing targets of Stevia, the purpose of this research paper is to explore the recent magnification of Stevia, a functional food ingredient used as an alternative to sugar, or other low calorie chemical sweeteners. Chemical Composition of Stevia As previously mentioned, Stevia is extracted from the Stevia Rebaudiana (Bertoni) plant. The chemical structure of Stevia leaves consists of glycosides, or combination of a non-carbohydrate group and an attached sugar. “All are glycosides of the common aglycone, or non-carbohydrate group, steviol. They differ in the number (typically three to five) and type of sugars attached” (7). In its leaves, there are nine to ten different steviol glycosides found that are accessible for extraction (8). Of those ten, Stevioside and Rebaudioside A are the only two major sweet tasting steviol glycosides that are used by food 3 manufacturers as a functional food component. But these steviol glycosides are not suitable to be added to food products in the raw leaf form. Rather, a refining procedure is necessary to generate a highly purified component of the Stevia leaves that is smoothly added to food products. In the first step, Stevia leaves are harvested and dried. Then the glycosides are extracted by steeping the dried leaves in hot water, a process much similar to the procedure used for tealeaf extraction. Next, the purification portion of the method begins when the Stevia extract is dissolved in ethanol and/or methanol where it crystallizes and is then filtered. An activated carbon is used to lighten the crystallized glycosides to a desired white or off white colored powder, closely resembling granulated sugar. The concentrate is finally dried and what remains is a high purity form of the essential Stevia extracts, Stevioside or Rebaudioside A (9). The finished refined product is a decolorized, purified and crystallized form of Stevia. And, it is freely soluble in water thus making it acceptable and suitable to add into consumer food products. The Generally Recognized as Safe (GRAS) policy makers recognizes five appropriate and acceptable highly refined preparations for Stevia in food products. Among them are McNeil Nutritionalswho produce Sun crystals, Cargill- the manufacturers of Truvia and Whole Earth Sweetener/Merisant-the creators of PureVia (10). 4 The Use of Stevia as a Functional Food From the shocking American health demographics, there comes an obvious consumer demand for lower calorie, safe tabletop “fake sugars.” Stevia is the newcomer to the industry, which is already thriving with aspartame, and sucralose. Like its fellow alternative sweeteners, Stevia offers a powerful sweetness without excess calories (4). But the main advantage that Stevia has over other sweeteners is that it originated from a plant rather than a chemical. Marketers have caught on to that fact and used it to their gain by pushing Stevia as the only “natural” alternative sweetener. They even back up this concept by using green packaging as opposed to the competition packet colors in pink, blue or yellow (NY TIMES). With that in mind, it is easy to see Stevia’s appeal to an open spectrum of food manufacturers. One physical characteristic of Stevia is that it is more stable in acidic beverages than other widely used sweeteners. By means of that advantage, Stevia is more than sufficient for the commercial production of soft drinks (7 on outline). Studies conducted by Cargill and Marisant have even revealed that the storage life of rebaudioside A in a carbonated citric acid beverage and a phosphoric acid beverage did not significantly degrade during prolonged storage at refrigeration, normal ambient, or elevated ambient temperatures. (GRAS) In terms of satisfactoriness when Stevia is used as to replace some or all of the sugar in a recipe, studies show that the recipe does not drastically affect the visual acceptability or physical characteristics of a food product (11 outline). 5 In one test where chocolates sweetened with stevioside were compared to conventional and commercial chocolates, there was no significant difference in acceptability (9 outline). And then in a double blind taste test involving baked goods, such as a cake, a cookie and shortbread, completely replacing sugar with Stevia demonstrated a different flavor with a mild variation in volume tenderness and texture. However, the overall product was generally accepted and satisfying (10 outline). There is an obvious recognition expansion for Stevia to be used as a functional food ingredient. But even with the rapid popularity growth, Stevia’s journey into our food products has not gone without controversy. For many years it was only permitted in America for use in dietary supplements. Steviol glycosides were approved to be used as a functional ingredient in dietary supplements since the Dietary Supplement Health and Education Act (DSHEA) passed in the United States in 1994 (Williams LD). At that time however, neither the extract nor any kind of refined component of Stevia was considered unobjectionably safe to be used in foods or beverages sold other than as a dietary supplement (CLOS JF). Although America was developing toleration for Stevia, in 2000 the European Commission refused to reason Stevia or stevioside as a novel food ingredient simply because there wasn’t enough critical scientific research reports. Among the various cited studies that did exist at the time, there were inconvenient discrepancies regarding possible toxilogical effects of the consumption of stevioside (4). On the other hand, in Southeast Asia in places like Japan, Korea 6 and China, Stevia and its refined components are recognized and sold as an acceptable sweetener (DE MELO9 on outline) The FDA’s response to the GRAS notice on Stevia and Stevia refined products was labeled as pending until September 9, 2009 (9). On that date, the case was closed and Stevia was accepted as safe for “scientific procedures, general-purpose sweetener in foods, excluding meat and poultry products, at levels determined by current good manufacturing practices.” After a great length of controversy, the FDA concluded, “there is no basis to object to the use of certain refined Stevia preparations in food. These refined Stevia preparations may be lawfully marketed and added to food products sold in the United States” (10). Conclusion With its overall flavor acceptability and stability, I believe the use of Stevia as a functional food ingredient has a valuable future. It may have taken a long time for many countries to decide, but today, throughout the world, Stevia has certainly earned the right to be considered a safe, natural sugar substitute and alternative sweetener. In my opinion, I believe that Stevia shows concrete rewards in the future treatment of type 2 Diabetes Mellitus. And with ongoing research and experiments, the applications of Stevia as a functional ingredient are immeasurable. Application to Dietary Practice 7 Having a comprehensive knowledge of Stevia based research is applicable to dietary practice in a variety of ways. Its advantages and drawbacks would clearly affect a great population being that currently, two thirds of adult Americans are overweight thus being at risk for a number of different harmful health conditions (1). With that being said, choosing Stevia offers a valuable alternative way to fight against those harmful health conditions like Obesity and Type 2 Diabetes Mellitus. What’s more, Stevia can be used as a substitute sweetener that provides powerful sweetness without raising blood sugar levels and without excess calories. For that reason, it can especially be recommended by dietitians to these two risk groups to help diminish their existence in our American population. The “fake sugar” industry has reached $1.2 billion-a-year. Newcomer Stevia has a distinct advantage to marketers; it comes from a plant so it can be marketed as a natural sweetener (NY Times). There are a variety of foods that have started to use Stevia Rebaudiana as a functional food ingredient because it is considered a natural sugar alternative. Stevia is appealing to many consumers and its popularity is growing along with the magnified competitive marketing of different sweeteners to the diabetic community. In addition, the popularity of Stevia is on the rise since the position of the American Dietetic Association holds that nonnutritive sweeteners, like Stevia, can safely be enjoyed by consumers when consumed in a diet that is guided by existing federal nutrition suggestions such as the Dietary References Intakes (2). 8 The list of products containing Stevia as a functional food ingredient is rather broad ranging from ice cream all the way to sports drinks. When compared to the price of sugar, the prices of crystallized packaged sweeteners made with Stevia are priced higher, but not priced impractically or unaffordable to most. For example, one hundred packets of sugar costs $1.69 whereas one hundred packets of PureVia, a crystallized form of Stevia extract, costs $3.29. It is almost impossible to overlook the fact that most of products using Stevia are sweet flavored beverages or beverage enhancers. This is undoubtedly due to the overwhelming demand of sweet drinks in America. At a whopping 36%, soda, energy drinks and sports drinks are the biggest source of added sugars in the diets of the U.S. Population for persons two years of age and older (Dietary Guidelines). These beverages range in price from under two dollars to almost seven dollars. When all is said and done, the use of Stevia as a functional food ingredient to sweeten and Stevia based research both prove very beneficial to dietetic practice. Selecting Stevia in place of sugar or a chemical sweetener demonstrates how a slight alteration in a person’s diet can drastically change their health risk. In fact, “replacing intake of added sugars with nonnutritive sweeteners could result in a deficit of 380 cal/day or 1 pound of weight loss in 9 to 10 days, if intake was at 95 g (24 tsp) daily” (8 Position of JADA). Stevia has the potential of altering the health risk demographics in America. 9 Below is a list of products that contain Stevia available to consumers found at Publix Super Market: 731 Duval Station Rd Jacksonville FL 32218 Product Name Description Truvia Crystallized, Refined Stevia rebaudiana Extract Crystallized, Refined Stevia rebaudiana Extract Crystallized, Refined Stevia rebaudiana Extract Combination of Sugar and Refined Stevia Extract Functional water beverage Functional water beverage Juice PureVia Stevia in the Raw Sun crystals Glaceau Vitamin Water Zero 0 Calorie SoBe Life Water Minute Maid Enhanced Pomegranate Tea Crystal Light Pure Fitness Odwalla Juice Powerade PLAY Powdered water supplement. Organic juice product Sports Drink Cost $6.29 for 80 packets; $3.99 for 40 packets $3.29 for 100 packets; $1.99 for 40 packets $3.59 for 50 packets; $6.99 for 9.7 oz $3.59 for 50 packets $1.49 each; $5.99 for 4 $1.59 each $3.69 $3.49 $2.99 Usually 4 for $5, Sale Price 10 for $10 References: 1. Anton SD, Martin CK, Han H, et al. Effects of stevia, aspartame, and sucrose on food intake, satiety, and postprandial glucose and insulin levels. Appetite. 2010; 55(1):37-43. 2. Dietary Guidelines for Americans 2010: U.S. Department of Agriculture, U.S. Department of Health and Human Services. Health.gov website. 2011. Available at http://www.health.gov/ dietaryguidelines. Accessed March 24, 2011. 3. Brandle JE, Telmer PG. Steviol glycoside biosynthesis. Phytochemistry. 2007; 68(14):1855-1863. 10 4. Chatsudthipong V, Muanprasat C. Stevioside and related compounds: Therapeutic benefits beyond sweetness. Pharmacol Ther. 2009; 121(1):41-54. 5. Walter JM, Soliah L. Objective Measures of Baked Products Made with Stevia. J Am Diet Assoc. 2010; 110(9, Supplement 1):A54-A54. 6. Geuns JMC. Stevioside. Phytochemistry. 2003; 64(5):913-921. 7. Williams LD, Burdock GA. Genotoxicity studies on a high-purity rebaudioside A preparation. Food and Chemical Toxicology. 2009; 47(8):1831-1836. 8. Brusick D. A critical review of the genetic toxicity of steviol and steviol glycosides. Food Chem Toxicol. 2008; 46(7):S83-S91. 9. Kraska RC, McQuate RS, Soni MG. GRAS ASSESSMENT OF HIGH PURITY STEVIOL GLYCOSIDES (> 97%). In: Food Usage Conditions for General Recognition of Safety. Bend, Oregon. 2010: 8-35. 10. FDA Basics. What refined Stevia preparations have been approved by FDA to be used as a sweetener? U.S Food and Drug Administration Department of Health & Human Services Web site. 2010. Available at http://www.fda.gov/AboutFDA/Transparency/Basics/ucm214865.htm. Accessed March 27, 2011. 11. Curry LL, Roberts A. Subchronic toxicity of rebaudioside A. Food and Chemical Toxicology. 2008; 46(7, Supplement 1):S11-S20. 12. Carakostas MC, Curry LL, Boileau AC, Brusick DJ. Overview: The history, technical function and safety of rebaudioside A, a naturally occurring steviol glycoside, for use in food and beverages. Food and Chemical Toxicology. 2008; 46(7, Supplement 1): S1-S10. 13. Position of the American Dietetic Association: Use of Nutritive and Nonnutritive Sweeteners. J Am Diet Assoc. 2004; 104(2):255-275. 14. de Melo, Lauro Luís Martins Medeiros, Bolini HMA, Efraim P. Sensory profile, acceptability, and their relationship for diabetic/reduced calorie chocolates. Food Quality and Preference. 2009; 20(2):138-143. 11 15. Kerzicnik L, Stendell N, McMuny M, Hagan D. Food Characteristics of Recipes Using Stevia Sweetener - A Proposed Herbal Sugar Substitute. J Am Diet Assoc. 1999; 99(9, Supplement 1):A29-A29. 16. Clos JF, DuBois GE, Prakash I. Photostability of Rebaudioside A and Stevioside in Beverages. J Agric Food Chem. 2008; 56(18):8507-8513. http://dx.doi.org/10.1021/jf801343e. 17. Koyama E, Sakai N, Ohori Y, et al. Absorption and metabolism of glycosidic sweeteners of stevia mixture and their aglycone, steviol, in rats and humans. Food and Chemical Toxicology. 2003; 41(6):875-883. 18. Gregersen S, Jeppesen PB, Holst JJ, Hermansen K. Antihyperglycemic effects of stevioside in type 2 diabetic subjects. Metab Clin Exp. 2004; 53(1):73-76. 19. Severson K. Showdown at the Coffee Shop. The New York Times. April 14, 2009: Dining & Wine 20. Kinghorn AD, Kim N. Sweet-tasting and sweetness-modifying constituents of plants. In: Atta-ur-Rahman, ed. Studies in Natural Products Chemistry. Vol Volume 27, Part 8. Elsevier; 2002:3-57. DOI: 10.1016/S1572-5995(02)80033-3. 12