Supporting Information
© Copyright Wiley-VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, 2008
Synthesis, Characterization and Application of Cationic Water-soluble
Oligofluorenes in DNA Hybridization Detection
Bin Liu*[a] and Shudipto Konika Dishari[a]
[a]
Department of Chemical and Biomolecular Engineering
National University of Singapore
Singapore 117576 (Singapore)
E-mail: cheliub@nus.edu.sg
Supporting information
Figure S1. The normalized absorption (top) and emission (bottom) spectra of fluorenyl oligomers in
methanol at [fluorene unit] = 3.0 × 10-6M.
Figure S2A. The absorption spectra of the trimer with increased amount of NaCl. [fluorene unit] = 3 × 106
M.
Figure S2B. The absorption spectra of the tetramer with increased amount of NaCl. [fluorene unit] = 3 ×
10-6M.
Figure S2C. The absorption spectra of the pentamer with increased amount of NaCl. [fluorene unit] = 3 ×
10-6 M.
Figure S2D. The absorption spectra of the hexamer with increased amount of NaCl. [fluorene unit] = 3 ×
10-6M.
Figure S2E. The absorption spectra of the heptamer with increased amount of NaCl. [fluorene unit] = 3 ×
10-6M.
Figure S2F. The absorption spectra of the polymer with increased amount of NaCl. [fluorene unit] = 3 ×
10-6 M.
Figure S3A. The emission spectra of the trimer with increased amount of NaCl. [fluorene unit] = 3 × 10-6
M.
Figure S3B. The emission spectra of the tetramer with increased amount of NaCl. [fluorene unit] = 3 ×
10-6 M.
Figure S3C. The emission spectra of the pentamer with increased amount of NaCl. [fluorene unit] = 3 ×
10-6 M.
Figure S3D. The emission spectra of the hexamer with increased amount of NaCl. [fluorene unit] = 3 ×
10-6 M.
Figure S3E. The emission spectra of the heptamer with increased amount of NaCl. [fluorene unit] = 3 ×
10-6 M.
Figure S3F. The emission spectra of the polymer with increased amount of NaCl. [fluorene unit] = 3 × 106
M.
Figure S4. The normalized absorption (top) and emission (bottom) spectra of fluorenyl oligomers 16-20
in buffer at [fluorene unit] = 3.0 × 10-6
[ssDNA-Fl] = 1.2 × 10-5 M.
M.
The absorption of Fl (ssDNA-Fl) is also shown in the bottom.
Figure S5. Emission spectra from solutions containing the hexamer/ssDNA-Fl (solid line),
hexamer/dsDNA-Fl (solid line with dots), polymer/ssDNA-Fl (dashed line), polymer/dsDNA-Fl (dashed
line with dots) in 25 mM phosphate buffer. Excitation wavelength is 386 nm for the hexamer and 411 nm
for the polymer. [ssDNA-Fl] or [dsDNA-Fl] = 1.0 × 10-8
M,
[fluorene unit] = 3 × 10-7
spectra are normalized with respect to the residual emission of each donor.
M.
The emission