University of Florida
Department of Microbiology and Cell Science
IMMUNOLOGY
PCB 4233 (3 credits)
SPRING SEMESTER 2015
COURSE DESCRIPTION:
PCB 4233 is a comprehensive course in basic immunology designed for advanced undergraduate
students. Emphasis will be placed on fundamental aspects of immunology, and its application to
real-world immunological research and concerns. Upon successful completion of the course,
students will have a solid immunological information foundation suitable for future educational
endeavors in the areas of biomedical research, or human/veterinary clinical applications. In
addition, students will have a fundamental understanding of basic immunological experimental
design. Student assessments in PCB 4233 will focus heavily on immunological facts, concepts,
and problem solving based on the application of concepts. PCB 4233 will be co-taught with
PCB 5235.
Prerequisite: MCB 3023 or equivalent.
instructor prior to enrolling in this course.
INSTRUCTOR
&
OFFICE HOURS:
Students lacking prerequisite should consult the
Dr. Joseph Larkin III
Microbiology & Cell Science Building, Room 1253
Phone: 352-392-6884
Mondays and Tuesdays 2:45-3:45
(Note: Students unable to meet these hours may schedule
appointments: email jlarkin3@ufl.edu. I will not be available for
scheduled appointments on February 27th or April 3rd)
TAs: Dr. Bhagyalaxmi Sukka-Ganesh
Ms. Zaynab Sidi Mohamed
Mr. Brandon Lam
Mr. William Allen
Phone: 352-392-9676
WEB PAGE:
E-learning (Sakaii)
LECTURES:
Monday, Wednesday, and Friday (4th period) 10:40-11:30 MCCA
G186. The majority of lectures administered throughout the
semester will be posted on the UF mediasite website after their
generation, and can be accessed through the Sakai (under heading
Lectures 2014).
Camtasia: Some lectures will be administered via the program Camtasia and will be accessed
through Sakaii
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MATERIALS:
REQUIRED TEXTBOOK:
Owen, Punt, and Stranford. 2013. Kuby Immunology. Seventh Edition. W. H. Freeman
and Company, New York. ISBN-13: 978-14292-1919-8.
5x7 Notecards: Not for a grade-but as a measure of participation- questions will be asked
at random in class. The name of the student, UFID, and answer will be placed on the
card and turned in.
Also please note the website: http://www.whfreeman.com/immunology7e. In addition,
valuable study tools can also be found at this website.
RECOMMENDED TEXTBOOK:
Murphy, K. 2012. Janeway’s Immunobiology 8th edition. Garland Science, Taylor &
Francis Group, LLC., New York. ISBN 978-0-8153-4243-4
Please note the website below. Essentially all animations used in class can be found on
this website.
http://www.youtube.com/user/garlandscience
PUNCTUALITY:
Class will begin promptly at 10:40 a.m. Please be on time and
seated, with your cell phone turned off. Should you arrive late to
class, please use the doors located at the rear of the room.
STUDENT LEARNING OUTCOMES: Upon successful completion students will • Be able to clearly state the role of the immune system
• Be able to compare and contrast the innate versus adaptive immune systems.
• Be able to articulate the roles of Toll-Like Receptors in the innate and adaptive immune
responses and specifically identify select receptors.
• Be able to compare and contrast humoral versus cell-mediated immune responses.
• Be able to distinguish various cell types involved in immune responses and associated
functions
• Be able to distinguish and characterize CD4+ T helper cell lineages TH1, TH2, TH17,
and regulatory T cell (Treg).
• Be able to distinguish and characterize antibody isotypes, development, and functions.
• Understand the role of cytokines in immunity and immune cell activation; and be able to
identify and characterize cytokines of particular immune importance.
• Understand the significance of the Major Histocompatibility Complex in terms of
immune response and transplantation.
• Be able to describe lymphocyte development and the expression of antigen receptors.
• Be able to characterize processes utilized by the immune system to mediate tolerance to
self-tissues
• Understand current scientific knowledge related to autoimmune disease etiologies
• Be able to articulate the ramifications of immunodeficiency with particular emphasis on
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acquired immunodeficiency.
STUDENT EVALUATION:
Class Attendance
is strongly encouraged; however recorded lectures will be available through the UF
Mediasite.
Larkin Clause: Although attendance is not required, should you score below 70% on any
exam, active participation in live classes will be evaluated.
Students are expected to read the Chapter in Kuby Immunology corresponding to lecture
prior to the lecture.
Students attending live lectures will at random be asked to
participate in discussions pertaining to prior readings, lectures, and online Assignments.
Online Assignments
Online Assignments will be based on readings from the text and the lectures. Online
assessments will consist of multiple choice questions and short answer. Online
assessments are available on the course website in Sakaii, with dues dates are listed
below. There will be 8 assignments (located in test and quizzes), due at 10pm on their
respective due dates, with the lowest 2 scores dropped. The six best Online Assignments
will be worth twenty points each for a total of 120 points.
Assignment 1
Assignment 2
Assignment 3
Assignment 4
Assignment 5
Assignment 6
Assignment 7
Assignment 8
Tuesday 1/13/15
Tuesday 1/20/15
Tuesday 2/10/15
Tuesday 2/17/15
Tuesday 3/17/15
Tuesday 3/24/15
Tuesday 3/31/15
Tuesday 4/14/15
Discussions
Peer to Peer interactions play an important role in the learning process. In order to
facilitate these interactions, 6 discussions will be assigned. Discussion entries will be
due at 10pm of the dates listed below. Four of these discussions will involve the
generation of a practice exam. Significantly, some questions posted on the discussion
board will likely be used on the exams. Therefore, it is to the advantage of all students to
review the questions posted on the student designed study guides.
Discussion #1
Discussion #2
Discussion #3
Discussion #4
Discussion #5
Discussion #6
Friday 1/16/15
Friday 1/23/15
Friday 2/20/15
Friday 3/20/15
Friday 3/27/15
Friday 4/17/15
Pre-Exam
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In preparation for future exams, a 15 question pre-exam, providing the types of questions
on exams and an overview of the material covered in the class, must be completed by
Friday, January 9, 2015 at 10pm. The pre-exam also allows the instructor to gauge
pre-knowledge of immunology. Participation in the pre-exam will require accessing
pretest in the Sakaii website. Preparation for the pretest is not required (or expected) as
participation in the pre-exam will result in 10 points of your final grade.
Examinations
Three (3) computer based, fifty minute In-Class exams (180 points each for a total of 540
points) will be administered consisting of approximately 30 multiple choice-format.
Although each exam will focus on a particular period of instruction, given the nature of
the subject matter, all examinations will be cumulative. Questions will be related to all
lectures given in the class, including guest lectures. Makeup exams will be given only
with advanced written permission, from the instructor, under the most extreme
circumstances. Only cases of serious illness, bereavement, or activities that fall under the
Twelve-Day rule will be considered for makeup. Makeup exams will likely be written
(not multiple choice). You must provide official documentation for all cases. Please
note: Professional Schools will reschedule interviews, if they conflict with an exam.
Take care of conflicts and other problems immediately. The Instructor reserves the right
to utilize Proctor U for makeup exams at cost to students.
A mandatory cumulative final examination (300 points) will be administered on
Wednesday, April 29th, 3p.m. -5 p.m. during final exams week. There is no make up for
the final.
We also request that students utilize restroom facilities prior to exam sessions so that
restroom usage will not occur during examination period.
Grading Format:
Online Assignments
Discussions
Pretest
In-Class Exams
Final Examination
Total
6@20 points each
6@5 points each
3@180 points each
120
30
10
540
300
1000
points
points
points
points
points
points
Final grades will be based on the following performance standard (1000 points total):
920 - 1000 points
900 - 919 points
870 - 899 points
830 - 869 points
800 – 829 points
760 - 799 points
700 - 759 points
650 - 699 points
600 – 649 points
=
A
AB+
B
BC+
C
D+
D
=
=
=
=
=
=
=
4
Less than 600 points
=
E
For questions regarding University of Florida Grading Policies please consult:
http://www.registrar.ufl.edu/catalog/policies/regulationgrades.html
COURSE SCHEDULE:
Week 1
Lect. #
W 01/07
1
F 01/09
2
Week 2
M 01/12
3
W 01/14
4
F 01/16
5
Week 3
W 01/19
M 01/21
6
F 01/23
7
Week 4
M 01/26
W 01/28
F 01/30
Week 5
M 02/02
8
W 02/04
9
F 02/06
10
Week 6
M 02/09
11
W 02/11
13
F 02/13
14
Week 7
M 02/16
16
W 02/18
17
F 02/20
18
Class Intro/ Historical Perspectives
Introduction to Immunity
chapter 1
ch. 1, ch5 (selected)
Hematopoiesis
Cells of the immune system
lymphoid organs and lymphatics
chapter 2
ch. 2 and pp 431-441
ch. 1, 11,
No Class (Martin Luther King, Jr. observed)
Adaptive immune response I
ch 1, 11, 12 (selected)
Adaptive immune response II
ch 16 (selected)
Catch up
Review
Exam 1
Receptors and Signaling
Structures of antibodies
B and T cell signaling
chapter 3
chapter 3
chapter 3
Cytokines and chemokines
Cytokines and chemokines
Innate immunity
chapter 4
chapter 4
chapter 5
Innate immunity
Introduction to Complement
Mechanisms/regulation of complement
chapter 5
chapter 6
chapter 6
Dr. Hoffmann
Week 8
M 02/23
W 02/25
F 02/27
Week 9
M 03/02
W 03/04
F 03/06
Week10
M 03/9
W 03/11
F 03/13
19
Mechanisms/regulation of complement
Review (chapters 3-6)
Exam 2 (chapters 3-6)
chapter 6
No Class
No Class
No Class
20
21
22
Experimental Techniques
Experimental Techniques II
Organization and expression of Ig genes
Week 11
5
Chapter 20
Chapter 20
Chapter 7
M 03/16
23
Immunoglobulin recombination
Chapter 7
W 03/18
24
Antigen Presentation/MHC
Chapter 8
F 03/20
25
Antigen Presentation/MHC
Chapter 8
Week 12
M 03/23
26
Antigen Presentation/MHC
Chapter 8
W 03/25
27
T cell development and function
Chapter 9
F 03/27
28
T cell development and function
Chapter 9
Week 13
M 03/30
29
T cell development and function
Chapter 9
W 04/01
30
T cell maturation/activation/diff
(Chapter 10)
F 04/03
Review
Week 14
M 04/06
Exam 3 (chapter 7-9, 20)
W 04/08
31
Mucosal Immunology
Dr. Volker Mai
F 04/10
32
Viral Immunology of the Mucosa
Dr. Stephanie Karst
Week15
M 04/13
33
Mucosal Immunology II
Dr. Langkamp-Henken
recorded lectures (2012 PCB5235 lectures April 13,
2012)
Also: www.youtube.com/watch?v=gnZEge78_78. W 04/15
34
Immunity and HIV
Dr. Yamamoto recorded lecture (2012 PCB 5235 lecture April 23, 2012)
F 04/17
Week 16
M 04/20
W 04/22
35
Tolerance
36
Catch up
Review Session
J Larkin
Cumulative Final exam: Wednesday, April 29th, 3:00 p.m.-5:00 p.m.
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Academic Honesty, Software Use, UF Counseling Services, Services for
Students with Disabilities
In 1995 the UF student body enacted a new honor code and voluntarily committed itself to the highest standards of
honesty and integrity. When students enroll at the university, they commit themselves to the standard drafted and
enacted by students.
In adopting this honor code, the students of the University of Florida recognize that academic honesty and integrity
are fundamental values of the university community. Students who enroll at the university commit to holding
themselves and their peers to the high standard of honor required by the honor code. Any individual who becomes
aware of a violation of the honor code is bound by honor to take corrective action. The quality of a University of
Florida education is dependent upon community acceptance and enforcement of the honor code.
The Honor Pledge: We, the members of the University of Florida community, pledge to hold ourselves and
our peers to the highest standards of honesty and integrity.
On all work submitted for credit by students at the university, the following pledge is either required or implied:
“On my honor, I have neither given nor received unauthorized aid in doing this assignment.”
The university requires all members of its community to be honest in all endeavors. A fundamental principle is that
the whole process of learning and pursuit of knowledge is diminished by cheating, plagiarism and other acts of
academic dishonesty. In addition, every dishonest act in the academic environment affects other students adversely,
from the skewing of the grading curve to giving unfair advantage for honors or for professional or graduate school
admission. Therefore, the university will take severe action against dishonest students. Similarly, measures will be
taken against faculty, staff and administrators who practice dishonest or demeaning behavior.
Students should report any condition that facilitates dishonesty to the instructor, department chair, college dean or
Student Honor Court.
(Source: 2009-2010 Undergraduate Catalog)
It is assumed all work will be completed independently unless the assignment is defined as a group project, in
writing by the instructor.
This policy will be vigorously upheld at all times in this course.
Software Use:
All faculty, staff and students of the university are required and expected to obey the laws and legal agreements
governing software use. Failure to do so can lead to monetary damages and/or criminal penalties for the individual
violator. Because such violations are also against university policies and rules, disciplinary action will be taken as
appropriate.
Campus Helping Resources
Students experiencing crises or personal problems that interfere with their general well-being are encouraged to
utilize the university’s counseling resources. Both the Counseling Center and Student Mental Health Services
provide confidential counseling services at no cost for currently enrolled students. Resources are available on
campus for students having personal problems or lacking clear career or academic goals, which interfere with their
academic performance. The Counseling Center is located at 301 Peabody Hall (next to Criser Hall). Student Mental
Health Services is located on the second floor of the Student Health Care Center in the Infirmary.
•
University Counseling Center, 301 Peabody Hall, 392-1575, www.counsel.ufl.edu
•
Career Resource Center, CR-100 JWRU, 392-1601 ext: 0, www.crc.ufl.edu/
•
Student Mental Health Services, Rm. 245 Student Health Care Center, 392-1171,
www.shcc.ufl.edu/smhs/
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Alcohol and Substance Abuse Program (ASAP)
Attention Deficit Hyperactivity Disorder (ADHD)
Center for Sexual Assault / Abuse Recovery & Education (CARE)
Eating Disorders Program
Employee Assistance Program
Suicide Prevention Program
Students with Disabilities
The Disability Resource Center coordinates the needed accommodations of students with disabilities. This includes
registering disabilities, recommending academic accommodations within the classroom, accessing special adaptive
computer equipment, providing interpretation services and mediating faculty-student disability related issues.
0001 Reid Hall, 392-8565, www.dso.ufl.edu/drc/
Should you have any complaints with your experience in this course please visit
http://www.distance.ufl.edu/student-complaints
to submit a complaint
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