As many of you know, as of May 1st 2013, Qiagen is no longer a UC Agreement supplier with products listed in . . We have collaborated with our Agreement suppliers Fisher Scientific, Life Technologies, & Bio-Rad, to identify alternative products that can be easily ordered from Bio Services, without having to order these products via an iRequest. These items are available for immediate pickup and are often cheaper than the Qiagen products. Click on the suppliers below to check out their offerings! Fermentas through Fisher Scientific Life Technologies Bio-Rad 5 Prime through Fisher Scientific If you have any technical questions regarding the products’ suitability or performance, please contact the sales associate listed at the end of each section. 250 rxns Cost per Fisher Fermentas through Fisher Size Prep/Rxn/ Fermentas # Description Unit/mL $1.47 Genejet Plasmid Miniprep $1.71 FERK0502 kit 50 preps Genejet Genomic DNA $2.53 FERK0722 purification kit 250 rxns 50 preps $2.06 FERK0691 50 preps $2.06 FERK0701 $5.90 FERK0731 12362 RNeasy Plus Mini Kit (50) 50 preps EndoFree Plasmid Maxi Kit 10 preps (10) $30.49 FERK0491 Genejet Gel extraction kit Genejet PCR Purification kit Genejet RNA purification kit Genejet Plasmid Maxiprep kit 28604 MinElute Gel Extraction kit 50 preps (50) FERK0831 Genejet Gel Extraction and DNA cleanup Micro kit 50 preps QIAGEN # QIAGEN Description Size 79654 QIAshredder (50) 50 preps 27104 QIAprep Spin Column (50) DNeasy Blood & Tissue Kit (250) QIAquick Gel Extraction Kit (50) QIAquick PCR Purification Kit (50) 50 preps 69506 28704 28104 74134 28004 MinElute PCR Purification kit (50) 50 preps DNeasy Blood & Tissue Kit (50) 50 rxns $2.29 $2.29 FERK0831 $2.94 FERK0721 FERK0731 28106 RNeasy Mini Kit (50) 50 preps $5.23 QIAquick Gel Extraction Kit (250) 250 preps $1.95 QIAquick PCR Spin Column (250) 250 preps $1.95 12163 Plasmid Maxi Kit (25) FERK0492 69504 74104 28706 25 preps $20.16 FERK0692 FERK0702 Cost per Prep/Rxn/ Unit/mL $1.26 $1.79 50 preps $0.56 50 preps $0.60 10 preps $3.94 50 preps $16.18 Genejet Gel Extraction and DNA cleanup Micro kit 50 rxns Genejet Genomic DNA purification kit 50 preps Genejet RNA purification kit 250 preps Genejet Gel extraction kit 250 preps Genejet PCR purification kit 25 preps Genejet Plasmid Maxiprep kit 250 preps $1.43 $1.43 $1.98 $3.94 $1.18 $1.26 $14.47 QIAGEN # 74106 12643 27106 12663 204143 30210 79254 76104 203445 203443 301305 301425 301307 301105 301427 203205 QIAGEN Description RNeasy Mini Kit (250) HiSpeed Plasmid Midi Kit (25) QIAprep Spin Column (250) HiSpeed Maxi Kit (25) QuantiTect SYBR Green PCR Kit (200) Ni-NTA Agarose (25ml) RNase-free DNase Set (50) RNAlater RNA Stabilization Reagent HotStarTaq Master Mix Kit (1000) HotStarTaq Master Mix Kit (250) SuperFect Transfection Reagent (1.2ml) Effectene Transfection Reagent SuperFect Transfection Reagent (4 x 1.2ml) PolyFect Transfection Reagent (1 ml) Effectene Transfection Reagent (4 x 1ml) HotStarTaq DNA Polymerase (1000) Cost per Fisher Fermentas through Fisher Size Prep/Rxn/ Fermentas # Description Unit/mL Genejet RNA purification 250 preps 4.60 FERK0732 kit Genejet Plasmid Midiprep 25 preps $12.16 FERK0481 kit Genejet Plasmid Miniprep 250 preps $1.44 FERK0503 kit 25 preps $24.31 Color HiGreen qPCR 200 rxns $1.92 FERK0362 Master Mixes 25 ml $10.55 1 each $89.50 FERK2981 50 ml $1.39 1000 u $0.69 FERK1052 250 u $0.78 FEREP0602 1.2 ml $204.62 FERR0531 1 ml $268.89 FERR0531 4 x1.2 ml $215.20 FERR0532 1 ml $136.31 FERR0531 4 x 1ml $233.41 FERR0532 Maxima Hotstart PCR Master Mix Maxima Hotstart Taq DNA Polymerase Turbofect Transfection Reagent Turbofect Transfection Reagent Turbofect Transfection Reagent Turbofect Transfection Reagent Turbofect Transfection Reagent 1000 u $0.49 FEREP0603 Maxima Hotstart Taq - Size Cost per Prep/Rxn/ Unit/mL 25 preps $3.05 250 preps $6.85 250 - $2.08 - 200 rxns - $1.44 - Rapidout DNA Removal Kit 50 $1.51 - - 500 units $0.59 500 units $0.44 1ml $2.18 1ml $2.18 5 x 1ml $182.92 1ml $2.18 5 x 1ml $182.92 5 x 500 units $0.38 QIAGEN # 201205 201443 201225 201203 201445 QIAGEN Description Taq DNA Polymerase (1000) Taq PCR Master Mix Kit (250) Taq PCR Core Kit (1000) Taq DNA Polymerase (250) Taq PCR Master Mix Kit (4 x 250) Angela Young Application Specialist -- Life Science Mobile: (858) 412-9428 UCSDhelp@FisherSci.com Size Cost per Fisher Fermentas through Fisher Prep/Rxn/ Fermentas # Description Unit/mL Size Cost per Prep/Rxn/ Unit/mL 1000 u $0.34 FEREP0405 Taq DNA Polymerase 5 x 500 units $0.15 250 u 1000 u $0.52 $0.43 FERK0171 FERK1072 PCR Master mix 2x Dream Taq Master mix 200 reactions 1000 $1.09 $0.36 250 u $0.37 FEREP0402 Taq DNA Polymerase 500 units $0.20 250 u x 4 $0.46 FERK0172 PCR Master mix 2x 1000 reactions $0.39 QIAGEN # QIAGEN Description Cost per Life Life Technologies Size Prep/Rxn/ Technologies # Description Unit/mL 50 preps $1.47 12183026 Homogenizer PURELINK QUICK MINI 50 50 preps $1.71 K210010 50 REACTIONS PureLink® Genomic DNA 250 rxns $2.53 K182002 Mini Kit 79654 QIAshredder (50) 27104 QIAprep Spin Column (50) DNeasy Blood & Tissue Kit (250) QIAquick Gel Extraction Kit (50) 50 preps $2.06 QIAquick PCR Purification Kit (50) 50 preps $2.06 69506 28704 28104 74134 50 preps Cost per Prep/Rxn/U nit/mL $1.45 50 preps $1.26 250 preps $2.13 Size K199925 S.N.A.P.Gel Purification Kit 25 rxns $2.10 CS12000 CST PCR CLEAN-UP KIT 100 PureLink® Pro 96 total RNA Purification Kit PURELINK PLASMID MAXI 10 10 REACTIONS PureLink® Quick Gel Extraction Kit 100 preps $1.70 4 Plates $2.88 10 preps $18.60 50 preps $1.63 50 preps $1.98 50 preps 50 preps $2.42 $4.54 250 preps $1.49 $1.70 50 preps $5.90 12173-011A 10 preps $30.49 K210006 50 preps $2.29 K210012 50 preps $2.29 K310050 50 rxns $2.94 50 preps $5.23 K182001 12183018A 250 preps $1.95 K210025 28106 RNeasy Plus Mini Kit (50) EndoFree Plasmid Maxi Kit (10) MinElute Gel Extraction kit (50) MinElute PCR Purification kit (50) DNeasy Blood & Tissue Kit (50) RNeasy Mini Kit (50) QIAquick Gel Extraction Kit (250) QIAquick PCR Spin Column (250) 250 preps $1.95 CS12000 12163 74106 Plasmid Maxi Kit (25) RNeasy Mini Kit (250) 25 preps $20.16 250 preps $4.60 K210007 12183025 CST PCR CLEAN-UP KIT 100 100 preps PURELINK PLASMID MAXI 25 25 REACTIONS 25 preps PureLink® RNA Mini Kit 250 preps 12643 HiSpeed Plasmid Midi Kit (25) K210014 PURELINK HIPURE FILTER MIDI 25 REACTIONS 12362 28604 28004 69504 74104 28706 25 preps $12.16 PureLink® PCR Micro Kit PureLink® Genomic DNA Mini Kit PureLink® RNA Mini Kit PureLink® Quick Gel Extraction Kit 25 preps $16.96 $3.74 $9.77 QIAGEN # QIAGEN Description Size Cost per Life Prep/Rxn/ Technologies # Unit/mL Life Technologies Description Size Cost per Prep/Rxn/U nit/mL 27106 QIAprep Spin Column (250) 250 preps $1.44 12663 HiSpeed Maxi Kit (25) 25 preps $24.31 K210007 204143 30210 QuantiTect SYBR Green PCR Kit (200) 200 rxns Ni-NTA Agarose (25ml) 25 ml $1.92 $10.55 4367659 R90115 PURELINK QUICK MINI 50 50 REACTIONS PURELINK PLASMID MAXI 25 25 REACTIONS Power SYBR® Green PCR Master Mix, 1-Pack (1 x 5 mL) Ni-NTA Agarose 1 each $89.50 12185010 PureLink® DNase Set 50 ml $1.39 AM7020 1000 u $0.69 11306016 $1.21 250 u $0.78 11306016 RNAlater® 100mL PLATINUM PCR SUPERMIX 100 RXN 100 reactions PLATINUM PCR SUPERMIX 100 reactions 100 RXN 1.2 ml $204.62 11668019 Lipofectamine 2000 1.5mL $315.70 1 ml $268.89 11668019 Lipofectamine 2000 1.5mL $315.70 4 x1.2 ml $215.20 11668019 Lipofectamine 2000 1.5mL (order 4) $315.70 1 ml $136.31 11668019 Lipofectamine 2000 1.5mL 4 x 1ml $233.41 11668019 1.5mL (order 4) $315.70 1000 u $0.49 10966018 1000 u $0.34 10342020 Lipofectamine 2000 Platinum Taq DNA Polymerase Taq DNA Polymerase, Recombinant 79254 76104 203445 203443 301305 301425 301307 301105 301427 203205 201205 RNase-free DNase Set (50) RNAlater RNA Stabilization Reagent HotStarTaq Master Mix Kit (1000) HotStarTaq Master Mix Kit (250) SuperFect Transfection Reagent (1.2ml) Effectene Transfection Reagent SuperFect Transfection Reagent (4 x 1.2ml) PolyFect Transfection Reagent (1 ml) Effectene Transfection Reagent (4 x 1ml) HotStarTaq DNA Polymerase (1000) Taq DNA Polymerase (1000) K210010 50 preps $1.26 25 preps $16.96 200 rxns 25mL $1.29 $9.46 50 preps $1.60 $1.08 $1.21 $315.70 120 reactions $0.69 500 units $0.36 QIAGEN # QIAGEN Description Size Cost per Life Prep/Rxn/ Technologies # Unit/mL 201443 Taq PCR Master Mix Kit (250) 250 u $0.52 10572014 201225 Taq PCR Core Kit (1000) 1000 u $0.43 10572014 201203 Taq DNA Polymerase (250) 250 u $0.37 Taq PCR Master Mix Kit (4 x 250) 250 u x 4 $0.46 10342053 201445 Heidi Brunell Sr. Sales Representative Consumables Mobile: 240 654 2988 Heidi.Brunell@lifetech.com 10572014 Life Technologies Description PCR SUPERMIX 100 REACTIONS PCR SUPERMIX 100 REACTIONS TAQ DNA POLYMERASE, CLONED 100 UN PCR SUPERMIX 100 REACTIONS Size Cost per Prep/Rxn/U nit/mL 100 reactions $1.07 100 reactions $1.07 100 units $0.35 100 reactions 1.07 79654 QIAshredder (50) 50 preps Cost per Prep/Rxn/ Bio-Rad # Unit/mL $1.47 27104 QIAprep Spin Column (50) 50 preps $1.71 69506 DNeasy Blood & Tissue Kit (250) 250 rxns $2.53 QIAGEN # 28704 QIAGEN Description Size $2.06 28104 74134 QIAquick Gel Extraction Kit (50) 50 preps QIAquick PCR Purification Kit (50) 50 preps RNeasy Plus Mini Kit (50) 50 preps 12362 EndoFree Plasmid Maxi Kit (10) 10 preps $30.49 28604 MinElute Gel Extraction kit (50) 50 preps $2.29 28004 MinElute PCR Purification kit (50) 69504 74104 $2.06 $5.90 28706 28106 12163 QIAquick PCR Spin Column (250) Plasmid Maxi Kit (25) 250 preps $1.95 25 preps $20.16 74106 RNeasy Mini Kit (250) 250 preps $4.60 - - - - 7326165 Freeze 'N Squeeze DNA Gel Extraction Spin Columns 7326300 PCR Kleen Spin Columns - 100 preps Freeze 'N Squeeze DNA Gel Extraction Spin Columns 7326300 PCR Kleen Spin Columns - - 7326820 Aurum Total RNA Mini Kit 7326166 Freeze 'N Squeeze DNA Gel Extraction Spin Columns 7326300 PCR Kleen Spin Columns Quantum Prep Plasmid 7326120 Midiprep Kit $ - 2.16 - 25 preps $ 2.27 25 preps - $ 3.13 - $ 6.46 $ 8.70 25 preps $ 2.27 25 preps - $ 3.13 - 50 preps $ 4.08 100 preps 25 preps $ $ 1.69 3.13 20 preps $ 8.70 Aurum Total RNA Fatty and 7326830 50 preps Fibrous Tissue Kit Quantum Prep Plasmid 7326120 20 preps Midiprep Kit $2.29 DNeasy Blood & Tissue Kit (50) 50 rxns $2.94 RNeasy Mini Kit (50) 50 preps $5.23 QIAquick Gel Extraction Kit (250) 250 preps $1.95 Size 7326400 Aurum Plasmid Mini Kit 7326165 50 preps Bio-Rad Description Cost per Prep/Rxn/ Unit/mL - QIAGEN # 12643 27106 12663 204143 30210 79254 QIAGEN Description HiSpeed Plasmid Midi Kit (25) Size 25 preps Cost per Prep/Rxn/ Bio-Rad # Unit/mL $12.16 QIAprep Spin Column (250) HiSpeed Maxi Kit (25) QuantiTect SYBR Green PCR Kit (200) Ni-NTA Agarose (25ml) RNase-free DNase Set (50) RNAlater RNA Stabilization Reagent HotStarTaq Master Mix Kit (1000) HotStarTaq Master Mix Kit (250) SuperFect Transfection Reagent (1.2ml) 250 preps $1.44 25 preps $24.31 200 rxns 25 ml 1 each $1.92 $10.55 $89.50 50 ml $1.39 1000 u $0.69 250 u $0.78 1.2 ml $204.62 1 ml $268.89 4 x1.2 ml $215.20 1 ml $136.31 4 x 1ml $233.41 203205 201205 Effectene Transfection Reagent SuperFect Transfection Reagent (4 x 1.2ml) PolyFect Transfection Reagent (1 ml) Effectene Transfection Reagent (4 x 1ml) HotStarTaq DNA Polymerase (1000) Taq DNA Polymerase (1000) 1000 u 1000 u $0.49 $0.34 201443 Taq PCR Master Mix Kit (250) 250 u $0.52 76104 203445 203443 301305 301425 301307 301105 301427 Bio-Rad Description 7326820 Aurum Total RNA Mini Kit Quantum Prep Plasmid Midiprep Kit 7326400 Aurum Plasmid Mini Kit Quantum Prep Plasmid 7326120 Midiprep Kit 7326828 DNase I 7326120 Size 50 preps $ 4.08 20 preps 100 preps $ $ 8.70 2.16 20 preps 1 vial - $ 8.70 $ 53.67 - 1708870 iTaq DNA Polymerase 250 u iTaq Universal SYBR Green 200 rxn 1725120 Supermix 1708870 iTaq DNA Polymerase 1703351 TransFectin Lipid Reagent 1703351 TransFectin Lipid Reagent 1703352 TransFectin Lipid Reagent 1703351 TransFectin Lipid Reagent 1703351 TransFectin Lipid Reagent 1708870 iTaq DNA Polymerase 1708870 iTaq DNA Polymerase 1708870 iTaq DNA Polymerase Cost per Prep/Rxn/ Unit/mL $ 0.85 $ 0.60 250 u $ 0.85 1 mL $ 318.07 1 mL $ 318.07 5 mL $ 234.58 1 mL $ 318.07 1 mL $ 318.07 250 u 250 u $ $ 0.85 0.85 250 u $ 0.85 QIAGEN # 201225 201203 201445 QIAGEN Description Taq PCR Core Kit (1000) Taq DNA Polymerase (250) Taq PCR Master Mix Kit (4 x 250) Leeanne Young Account Manager Mobile: 619-761-9567 leeanne_young@bio-rad.com 1000 u 250 u Cost per Bio-Rad Description Prep/Rxn/ Bio-Rad # Unit/mL $0.43 1708870 iTaq DNA Polymerase $0.37 1708870 iTaq DNA Polymerase 250 u x 4 $0.46 Size 1708870 iTaq DNA Polymerase 250 u 250 u Cost per Prep/Rxn/ Unit/mL $ 0.85 $ 0.85 250 u $ Size 0.85 79654 QIAshredder (50) 50 preps Cost per 5 Prime Prep/Rxn/U Fisher # nit/mL $1.47 27104 QIAprep Spin Column (50) DNeasy Blood & Tissue Kit (250) QIAquick Gel Extraction Kit (50) QIAquick PCR Purification Kit (50) 50 preps $1.71 2300100 PerfectPrep Spin Mini Kit 250 rxns $2.53 2301910 50 preps $2.06 2300500 PerfectPure DNA Blood Kit 50 preps Agarose GelExtract Mini Kit 50 preps $1.54 50 preps $2.06 2300600 PCRExtract Mini Kit $1.54 RNeasy Plus Mini Kit (50) EndoFree Plasmid Maxi Kit (10) MinElute Gel Extraction kit (50) MinElute PCR Purification kit (50) DNeasy Blood & Tissue Kit (50) 50 preps $5.90 2400000 PerfectPro Ni-NTA Agarose 10ml Call Core for Price 10 preps $30.49 2302410 $7.83 50 preps $2.29 2300120 50 preps $2.29 2300500 PerfectPure RNA Tissue Kit 50 preps PerfectPrep Endofree 10 preps Maxi Kit Agarose GelExtract Mini Kit 50 preps 50 rxns $2.94 2300600 PCRExtract Mini Kit $1.54 RNeasy Mini Kit (50) QIAquick Gel Extraction Kit (250) QIAquick PCR Spin Column (250) Plasmid Maxi Kit (25) 50 preps $5.23 2301910 PerfectPure DNA Blood Kit 50 preps Call Core for Price 250 preps $1.95 2302410 $7.83 250 preps $1.95 25 preps $20.16 2300510 2300610 RNeasy Mini Kit (250) HiSpeed Plasmid Midi Kit (25) 250 preps $4.60 2300120 PerfectPure RNA Tissue Kit Agarose GelExtract Mini Kit PCRExtract Mini Kit PerfectPrep Endofree Maxi Kit 25 preps 2302440 PerfectPure RNA Tissue Kit 50 preps QIAGEN # 69506 28704 28104 74134 12362 28604 28004 69504 74104 28706 28106 12163 74106 12643 QIAGEN Description Size $12.16 5 Prime through Fisher Description Size Cost per Prep/Rxn/Unit/mL - - - 50 preps 50 preps 50 preps 50 preps $1.61 Call Core for Price $16.65 $1.54 200 preps $1.48 200 preps $1.48 10 preps $16.65 $8.95 QIAGEN # QIAGEN Description Size Cost per 5 Prime Prep/Rxn/U Fisher # nit/mL 5 Prime through Fisher Description PerfectPrep Endofree Maxi Kit Size Cost per Prep/Rxn/Unit/mL 27106 QIAprep Spin Column (250) 250 preps $1.44 2300120 12663 HiSpeed Maxi Kit (25) QuantiTect SYBR Green PCR Kit (200) Ni-NTA Agarose (25ml) 25 preps $24.31 2300110 200 rxns 25 ml $1.92 $10.55 2300120 2500120 RNase-free DNase Set (50) RNAlater RNA Stabilization Reagent HotStarTaq Master Mix Kit (1000) HotStarTaq Master Mix Kit (250) SuperFect Transfection Reagent (1.2ml) Effectene Transfection Reagent SuperFect Transfection Reagent (4 x 1.2ml) PolyFect Transfection Reagent (1 ml) Effectene Transfection Reagent (4 x 1ml) HotStarTaq DNA Polymerase (1000) Taq DNA Polymerase (1000) 1 each $89.50 50 ml $1.39 2200410 1000 u $0.69 250 u $0.78 1.2 ml $204.62 - - - - 1 ml $268.89 - - - - 4 x1.2 ml $215.20 - - - - 1 ml $136.31 - - - - 4 x 1ml $233.41 - - - 1000 u $0.49 1000 u $0.34 204143 30210 79254 76104 203445 203443 301305 301425 301307 301105 301427 203205 201205 - PerfectPrep Spin Mini Kit PerfectPrep Endofree Maxi Kit RNase-free DNase Set - 10 preps $16.65 200 preps $0.99 10 preps 50 rxns - $16.65 Call Core for Price Call Core for Price 2200840 5 Prime HotMasterMix 1000 u RealMasterMix Fast SYBR 250 rxns ROX Kit 2200400 5 Prime HotMasterMix Call Core for Price 100 u Call Core for Price 2200320 HotMaster Taq DNA Polymerase 1000 u Call Core for Price 2200020 Taq DNA Polymerase 1000 u Call Core for Price QIAGEN # 201443 201225 201203 201445 QIAGEN Description Size Taq PCR Master Mix Kit (250) Taq PCR Core Kit (1000) 250 u 1000 u Taq DNA Polymerase (250) 250 u Taq PCR Master Mix Kit (4 x 250) 250 u x 4 Angela Young Application Specialist -- Life Science Mobile: (858) 412-9428 UCSDhelp@FisherSci.com Cost per 5 Prime Prep/Rxn/U Fisher # nit/mL 5 Prime through Fisher Description Size Cost per Prep/Rxn/Unit/mL $0.52 $0.43 2200100 - 5 Prime MasterMix - 100 rxns - Call Core for Price - $0.37 2200010 Taq DNA Polymerase 250 u Call Core for Price $0.46 2200110 5 Prime MasterMix 1000 rxns Call Core for Price