Lab 2 next week on Protists (Chapter 28) • There will be a short quiz on this week’s material and next week’s lab handout • Bring a pencil for drawing • Bring the “Atlas” • Bring your text if you can bear to. • Overview: They’re (Almost) Everywhere! • Prokaryotes are microscopic, unicellular, numerous. • 1 g of soil contains millions/billions of prokaryote cells. • There are more bacterial cells in your body than human cells • Prokaryotes are fantastically diverse – genetically, ecologically, etc. Chapter 27 -- Prokaryotes A key to types of cells: 1 Cells internally complex, with nuclei and membrane-bound organelles ……………………………………….. Eukaryotes 1’ Cells internally simple, lacking nuclei and membrane-bound organelles …………………………………………Prokaryotes • Prokaryotic cells have a variety of shapes: spheres (cocci), rods (bacilli), and spirals 1 μm Figure 27.2a–c (a) Spherical (cocci) 2 μm (b) Rod-shaped (bacilli) 5 μm (c) Spiral The capsule of many prokaryotes surrounds the cell wall • The prokaryote cell wall maintains cell shape and provides physical protection. • Prokaryote cell walls contain polysaccharides and proteins. • Cell walls are produced outside the cell (plasma) membrane and are also found in fungi, plants and “algae”. 1 Some prokaryotes have fimbriae which facilitate attachment • Some bacteria are motile, propel themselves by flagella • Taxis – movement toward or away from a stimulus Flagellum Filament 50 nm Cell wall Hook Basal apparatus Figure 27.6 Compartmentalization of function in prokaryotes • Respiration and photosynthesis may occur on specialized membranes. • Most of the DNA is in a single circular “chromosome”. • Some DNA may be in circular plasmids Prokaryote metabolisms are diverse …. • Phototrophs use light as an energy source • Chemotrophs use chemical compounds as an energy source • Autotrophs can use CO2 as their sole source of carbon • Heterotrophs need more complex compounds as their source of carbon Plasma membrane Reproduction and adaptation • Prokaryotes reproduce rapidly by binary fission. • Conjugation results in horizontal gene transfer. • Prokaryotes evolve rapidly in response to environmental challenges, e.g. antibiotics … and here are the possibilities: • • • • Photoautotrophs Chemoautotrophs Photoheterotrophs Chemoheterotrophs 2 Phylogenetic classification of organisms based on molecular (genetic) evidence Eukaryotes Domain Eukarya Nanoarchaeotes Korarchaeotes Gram-positive bacteria Cyanobacteria Spirochetes Chlamydias Delta Epsilon Beta Gamma Alpha Proteobacteria Universal ancestor Figure 27.12 Some biological roles of prokaryotes A Modern Classification of Life • Domain Bacteria – prokaryotes, many well known and familiar species. • Domain Archaea – prokaryotes, discovered recently, many inhabit extreme environments. • Domain Eukarya – eukaryotes (animals, fungi, plants, “algae”, “protozoa”. Domain Archaea Domain Bacteria – on the basis of appearance, metabolism, structure, response to stains, etc. – or on the basis of their DNA or RNA. This produces a phylogenetic (evolutionary) classification Crenarchaeotes • Prokaryotes can be classified Euryarchaeotes Prokaryote classification • • • • Decomposers Nitrogen fixers Symbionts Pathogens 3