Cell Membranes and Transport Honors Biology Chapter 5 Important Root Words Root Word Meaning hypo below hyper above ton tension -ic pertaining to iso lysis cytendoexo-osis phag plasm same loosening, splitting cell within outside condition feed, eat to form or develop 1 What is homeostasis? Review this term with your group. Come up with a great definition! Relate this term (and its importance) to cells! How do cells maintain homeostasis? The Cell and its Environment Biological Balance – Homeostasis • Every organism must constantly adjust in • • order to survive Homeostasis occurs at all levels of living things For homeostasis to occur, the cell must keep necessary balance within the limits of environmental changes 2 Movement of Materials Brownian Motion: Constant movement of molecules Diffusion: Process by which molecules of a substance move from areas of high concentration to areas of low concentration. • One of the major means of molecular transport in cells Movement of Materials Concentration gradient: Difference in concentration of molecules across a membrane What factors affect the rate of diffusion? How would you design an experiment to test these factors? 3 What is the structure of cell membranes? Draw a basic diagram of the cell membrane with your group. • What are the important • • components? Why does it have this structure? Can you name any important characteristics? Structure of the Cell Membrane Double layer of lipids with proteins that control passage of some molecules into and out of the cell “Fluid-Mosaic” Model Selectively Permeable: Controls passage of materials into and out of the cell Cell Membrane Animation 4 Characteristics of cell membranes Factors that let selectively permeable membranes operate: • Structure of Plasma Membrane itself • • • • • Water diffuses through openings called pores located in cell membranes • Process of water diffusing into cells is called osmosis Size of Particles • Oxygen and Carbon Dioxide dissolve and pass through lipid layers • Water molecules do dissolve and pass through lipid Chemical make-up of particles Whether or not particles are soluble in water Conditions inside and outside the cell Osmosis and Living Cells Equilibrium: When concentrations of two solutions separated by a membrane is the same Water continues to move back and forth across a membrane until equilibrium is reached Concentration of water on each side of membrane is determined by number of solutes (dissolved solids) in that water solution 5 Comprehension Check Here is a sample cell in a beaker of water, we placed monosaccharide and polysaccharide inside the cell. We also took iodine (the indicator for ____?_____) and placed it into the beaker. • What diffused into/out of the dialysis tubing and why? • How did you know what had moved through the tubing? Isotonic Solutions A simple rule to remember is “Solute Sucks” Isotonic Solution: Concentration of solutes outside of cell membrane is same as that inside of cell membrane • Ex: Plasma in blood is isotonic with the red blood cells 6 Hypotonic Solutions Hypotonic Solutions: Concentration of solutes outside the cell membrane is lower than inside the cell membrane • Ex: Freshwater plants and animals living in lakes and streams where water is constantly flowing Hypertonic Solutions Hypertonic Solutions: The concentration of solutes outside the cell is greater than that inside the cell • Ex: drinking sea water causing cells to lose water 7 Plant versus Animal Cells Review the major differences between plant cells and animal cells with your group. • • How would a plant cell react to a hypertonic solution? How is this different than an animal cell? How would an animal cell react to a hypotonic solution? How is this different than a plant cell? Would changes in solutions affect unicellular or multicellular organisms more? 8 Types of solutions • • • • Turgor Pressure: Pressure that builds in a plant cell as a result of osmosis Cytolysis: The bursting of an animal cell membrane in a hypotonic environment Plasmolysis: The shrinking of cells as a result of water loss. Contractile Vacuoles: Tiny vacuoles found in one-celled animals which pump out excess water Passive and Active Transport Talk to your group about the difference between passive and active transport. Decide what these terms mean and come up with reasons why passive and active transport would be used by cells. 9 Passive and Active Transport Both diffusion and facilitated diffusion are forms of passive transport of molecules down their concentration gradient, while active transport requires an investment of energy to move molecules against their concentration gradient. Passive Transport Passive Transport: Passing of materials into and out of the cell without the use of energy (ATP) • Diffusion • Facilitated Diffusion: Proteins assist certain molecules to move through cell membrane • Carrier Molecules: The proteins that assist in facilitated diffusion • Ex: Glucose molecules going in/out of the cell • Ion Channels: Membrane proteins that help such ions as Na+, Ca2+, Cl-,K+, to cross the cell membrane. These ions are not soluble in lipids and need assistance to cross. 10 Active Transport Transport of materials into or out of the cell with the use of cell energy (ATP) • Active transport using carrier molecules involves actual movement of materials in a cell from regions of low concentrations to regions of higher concentrations. • Ex: Moving glucose from blood stream into our liver cells Bulk Transport A type of active transport where materials into or out of the cell that cannot normally pass through the cell membrane Exocytosis: The bulk transport of substances out of the cells Endocytosis: Bulk transport of substances into the cell • Phagocytosis: solids or larger particles • Pinocytosis: liquids 11 Comprehension Check Requires Energy Requires NO Energy Place the following terms into one of the boxes above: Diffusion Osmosis Active Transport Facilitated Diffusion Passive Transport Exocytosis Endocytosis Sodium-Potassium Pump Video Real World Example: Active Transport Sodium Potassum Pump The movement of Na+ and K+ against their concentration gradients Typically, an animal cell has higher concentrations of K+ and lower concentrations of Na+ inside the cell. The sodium-potassium pump uses the energy of one ATP to pump three Na+ ions out and two K+ ions in.\ At top speed, the Na+/K+ Pump can transport about 450 Na+ and 300 K+ ions per second Used in the conduction of nerve 12