MLT 210 Flashcards UNIT FOUR 1. A routine urinalysis can provide information regarding : A. The state of the kidney B. The state of the urinary tract C. Metabolic or systemic (nonrenal) disorders D. All of the above 2. When performing a urinalysis, all of the following factors are important except knowledge of : A. Common interfering substances (false-negative and false-positive reactions) B. Any critical steps or considerations in the procedure C. The clinical application of the substance being tested for and its correlation to other findings in the urinalysis D. Appropriate treatment protocols for disorders detected by the urinalysis 3. The functional (working) unit of the kidney is the: A. Nephron B. Glomerulus C. Cortex D. Medulla 4. Functions of the kidney include all of the following except: A. Removal of waste products B. Processing of nutrients C. Retention of nutrients D. Acid-base, water, and electrolyte balance 5. The function of the distal convoluted tubule of the kidney is to: A. Reduce the volume of the urine B. Maintain water and electrolyte balance C. Remove acid from the body D. Both B and C 6. Important factors in the collection and handling of urine include all of the following except the: A. Container used B. Collection procedure C. Conditions of storage and preservation from the time of collection until the specimen is tested D. Use of a sterile container 7. The most desirable type of routine urinalysis specimen is: A. Sterile B. First, early morning C. Random, mid-day D. 24-hour 8. Urine should be examined ____ collection. A. Immediately after B. Within 30 minutes, if not refrigerated or suitably preserved at C. Within 2 hours if kept at room temperature after D. Within 24 hours if refrigerated D D A B D D B B MLT 210 Flashcards UNIT FOUR 9. The major contributing factor to the decomposition of urine is: A. The action of bacteria B. The formation of crystals C. Fading of color D. Change in pH 10. The minimum acceptable volume of urine for routine urinalysis is ____ mL. A. 5 B. 12 C. 25 D. 50 11. The first part of the routine urinalysis is the ____ examination. A. Physical B. Chemical C. Microscopic D. Either B or C 12. Urine specimens must be fresh and centrifuged for reagent strip testing. A. True B. False 13. The longer a reagent strip is in contact with a urine specimen, the better the results that will be observed. A. True B. False 14. Reagent strips will deteriorate if the cap is kept open for a prolonged period. A. True B. False 15. Reagent strips will deteriorate if exposed to moisture or heat. A. True B. False 16. Reagent strips must be used within 1 year of opening the container. A. True B. False 17. Reagent strips (e.g., Chemstrips) have a stable reaction color for up to 5 minutes. A. True B. False 18. The normal pH of freshly voided urine is: A. 4 or 5 B. 5 or 6 C. 6 or 7 D. 7 or 8 A B A B B A A B B B MLT 210 Flashcards UNIT FOUR 19. In the detection and diagnosis of renal disease, the most significant single finding probably is A. Glucose B. Protein C. pH D. Ketones 20. In testing for glucose in the urine, all of the following are true except: A. If the blood glucose concentration becomes too high (usually greater than 180-200 mg/dL), the excess glucose will not be reabsorbed into the blood and will be eliminated from the body in the urine. B. The renal threshold is the lowest blood glucose concentration that will result in glycosuria. C. The most common condition in which the renal threshold for glucose is exceeded is diabetes mellitus. D. The occurrence of glycosuria is diagnostic of diabetes mellitus. 21. Reagent strips for glucose detection use a(n): A. Enzyme, peroxidase B. Chemical, hydrogen peroxide C. Chemical, copper sulfate D. Both A and B 22. A false-positive urinary glucose result can be observed if: A. Contamination by bleach or other strong oxidizing agents occurs B. Reagent strips are exposed to air by improper storage C. Large urinary concentrations of ascorbic acid are present D. Both A and B 23. Ketone bodies can be detected in urine in: A. Lack of adequate insulin control B. Starvation C. Extreme dieting D. All of the above 24. The presence of bilirubin in the urine is an early sign of: A. Liver disease B. Pancreatic cancer C. Intestinal inflammation D. Various infectious diseases 25. Specimens for bilirubin testing must be A. Absolutely fresh B. Less than 2 hours old C. Less than 8 hours old D. Less than 24 hours old 26. 0-5/hpf (high-power field) red blood cells A. Normal B. Abnormal 27. >10 white blood cells/hpf A. Normal B. Abnormal B D D D D A A A B MLT 210 Flashcards UNIT FOUR 28. Moderate number of squamous epithelial cells A. Normal B. Abnormal 29. Moderate number of renal epithelial cells A. Normal B. Abnormal 30. Few bacteria in a fresh, nonsterile specimen A. Normal B. Abnormal 31. Hyaline cases (20-30/lpf [low-power field]) A. Normal B. Abnormal 32. Structures that can be confused with red blood cells include: A. Leukocytes (white blood cells) B. Yeast C. Calcium oxalate crystals D. Both B and C 33. The least pathological type of cast is: A. Hyaline B. Finely granular C. Waxy D. Fatty 34. Upon standing, red blood cells either swell or become crenated. A. True B. False 35. White blood cells rapidly deteriorate in hypotonic solutions. A. True B. False 36. Casts disintegrate as urine becomes alkaline. A. True B. False 37. The growth of bacteria in urine produces a more acidic solution. A. True B. False MATCHING A. Any increase in urine volume, even if the increase is only temporary B. Complete absence of urine formation C. Abnormally small amount of urine, less than 500 mL/24 hr D. Excretion of more 400 mL urine at night E. Consistent elimination of an abnormally large volume of urine 38. Polyuria 39. Diuresis 40. Oliguria 41. Anuria 42. Nocturia A B A B D A A A A B ANSWER 38. E 39. A 40. C 41. B 42. D MLT 210 Flashcards UNIT FOUR 13. 14. 15. 16. 43. Proteins are present without the presence of albumin A. False positive B. False negative 44. A blue color forms for the protein reaction, if the urine is exposed to the reagent strip for too long A. False positive B. False negative 45. The reaction for protein may be produced, if a urine specimen is exceptionally alkaline or highly buffered A. False positive B. False negative 46. In the reaction to detect blood in the urine, microbial peroxidase activity associated with urinary tract infection can produce A. False positive B. False negative 47. In the detection of blood in the urine, ascorbic acid in urine specimens containing more than 25 mg/dL A. False positive B. False negative 48. Medications (e.g., azo-containing compounds or dyes that color urine red or that turn red in an acidic medium when testing for nitrite for the detection of bacteria in urine) A. False positive B. False negative 49. Urinary preservatives in urine testing for leukocyte esterase A. False positive B. False negative MATCHING A. Exposure to nephrotoxic substances B. Urinary tract infection C. Acute pyelonephritis D. Glomerular damage 50. White blood cell casts 51. Epithelial casts 52. Red blood cell casts and red blood cells 53. Bacterial casts B A A A B A A ANSWER 50. B 51. A 52. D 53. C MLT 210 Flashcards UNIT FOUR MATCHING A. Found in acid urine B. Envelope-shaped, glistening and colorless C. Coffin lids D. Colorless slender needles that are strongly birefringent with polarized light E. Yellow or reddish-brown shapes (e.g., lemon- or barrel-shaped forms) 54. Amorphous urates 55. Uric acid 56. Calcium oxalate 57. Triple phosphate 58. Acyclovir 1.My 59. Mycology is the study of: A. Viruses B. Parasites C. Yeasts and molds D. Bacteria 2.What 60. What is the name of the type of infection that is acquired in a hospital? A. Opportunistic infection B. Normal flora C. Community acquired infection D. Nosocomial infection 61. All of the following are structures found in prokaryotic organisms except: A. Mitochondria B. Capsules C. Ribosomes D. Cell wall 62. Which of the following systematic designations is correct? A. Staphylococcus aureus B. Staphylococcus Aureus C. Staphylococcus aureus D. Staphylococcus Aureus 63.What is the biosafety level of most clinical microbiology laboratories? A. Biosafety Level 1 B. Biosafety Level 2 C. Biosafety Level 3 D. Biosafety Level 4 64.Which of the following is a false statement when considering proper disinfection processes? A. The disinfectant should be dried immediately for best results. B. Phenol and diluted bleach are two effective disinfectants. C. The work area should be disinfected prior to use and again when work is completed. D. Disinfection is an ongoing process. ANSWER 54. A 55. E 56. B 57. C 58. D C D A C B A MLT 210 Flashcards UNIT FOUR 65. The technique of killing or destroying all microorganisms including bacterial spores (endospores) is called: A. Antisepsis B. Sterilization C. Physical disinfection D. Chemical disinfection 66. Which of the following is a sterilization procedure that uses moist heat and how is this method effective in killing bacterial spores? A. Filtration; filters all microbes larger than the filter pore size B. Boiling; kills all microorganisms and spores at 100C (boiling) C. Hot-air chamber; kills all microorganisms and bacterial spores at 171C for at least 1 hour D. Autoclaving; kills all microorganisms and bacterial spores at 121C for 15 minutes at 15 lb above atmospheric pressure 67. Sending appropriately labeled and collected specimens before antibiotic therapy to the laboratory is important because: A. Pathogenic bacteria will be hard to recover because of the effects of the antibiotic. B. Improperly labeled specimens will cause erroneous results and possible serious consequences for the patient. C. Collection of specimens from around the wound area can result in contamination of the culture. D. Collection of a sputum specimen contaminated with saliva could yield a culture result that reflects normal mouth flora. E. All of the above. 68.Which of the following specimens can be refrigerated? A. Urine for routine culture B. Cerebrospinal fluid C. Genital culture D. Culture for meningitis 69.Which of the following methods is appropriate to collect blood for culture? A. Cleaning the venipuncture site with 70% alcohol B. Cleaning the venipuncture site with 70% alcohol, followed by povidoneiodine, moving in a circular motion from the intended venipuncture site outward C. Cleaning the venipuncture site with 70% alcohol, followed by povidoneiodine, moving in a circular motion beginning at the periphery of the area to be cleaned inward toward the venipuncture site D. All of the above methods can be used for collection of blood for culture 70. Which of the following specimen types for culture can be collected in a clean, nonsterile container? A. Urine B. Stool C. Cerebrospinal fluid B D E A B B MLT 210 Flashcards UNIT FOUR 71. A growth of bacteria that began as one parent bacterium and divided multiple times to become a visible aggregate is called a: A. Culture B. Pure culture C. Slant culture D. Colony 72. .Routine microbiology laboratories have incubators that are set at all of the following temperatures except: A. 30 C B. 35 C C. 42 C D. 50 C 73. Which of the following is a differential stain used routinely in microbiology laboratories? A. Methylene blue B. Flagella stain C. Gram stain D. Capsule stain 74. The prefix strepto- refers to bacteria in which of the following groupings? A. Clusters B. Pairs C. Chains D. Singular 75. Why do gram-positive bacterial cells retain the crystal violet–iodine complex when the decolorizer is added? A. Gram-positive cells have an outer membrane that traps the complex. B. Gram-positive bacterial cells have a thinner peptidoglycan layer with teichoic acid cross-linkages. C. Gram-positive bacterial cells have a thicker peptidoglycan layer with teichoic acid cross-linkages. D. Gram-positive bacterial cells have a thick capsule layer that traps the complex. 76. What is the color of gram-negative bacterial cells after the decolorization step? A. Purple/blue B. Red/pink C. Iodine yellow D. Colorless 77. Which of the following is the “cold” method for staining acid-fast bacteria? A. Ziehl-Neelsen B. Kinyoun C. Fluorochrome D. All of the above D D C C C D B MLT 210 Flashcards UNIT FOUR Which 78. Which of the following bacteria are typically stained using the acid-fast technique? A. Mycobacteria B. Staphylococci C. Streptococci D. Escherichia coli Which 79. Which type of microscope is used to observe acid-fast bacteria when they are stained with auramine and rhodamine? A. Brightfield B. Electron C. Fluorescent D. All of the above 22.Gro 80. Growth of several colonies originating from a single colony, and thus a single cell, is known as a: A. Subculture B. Primary culture C. Mixed culture D. Pure culture Which 81. Which of the following descriptions refer to colony morphology? A. Cocci in chains B. Cocci in pairs C. Shiny, wet, or mucoid D. Yellow, dry, rough E. A and B AB. C and D Which 82.Which of the following is an organism that can grow in the presence or absence of oxygen? A. Aerobe B. Anaerobe C. Facultative anaerobe D. Microaerophile Where 83. Where do strict aerobes grow in thioglycolate broth? A. At the top B. In the middle C. At the bottom D. Throughout the broth Media 84. Media that have dyes, antibiotics, or other chemical compounds added to inhibit certain bacteria while allowing others to grow is called _____ media. A. Enrichment B. Supportive C. Selective D. Differential A C D AB C A C MLT 210 Flashcards UNIT FOUR Media 85.Media that contain factors such as lactose with an indicator present to give distinctive, recognizable characteristics is called _____ media. A. Enrichment B. Supportive C. Selective D. Differential Chocol 86. Chocolate agar contains all of the following except: A. Antibiotics B. X factor C. V factor D. Lysed blood 29. 87. Which of the following is a selective and differential medium for the recovery of gram-positive organisms? A. Columbia colistin-nalidixic acid agar with blood (CNA) B. Eosin-methylene blue agar (EMB) C. MacConkey agar (MAC) D. Xylose-lysine desoxycholate agar (XLD) 30. 88. If a microbiologist wants to add a selective and enriched medium for the recovery of Neisseria gonorrhoeae from genital specimens, which of the following would be appropriate? A. Chocolate agar (CHOC) B. Sheep blood agar (SBA or BA) C. Modified Thayer-Martin agar (MTM) D. Phenylethyl alcohol agar (PEA) 89. A microbiologist observes colonies on Hektoen enteric agar (HE) that appear green. Which of the following organisms should the microbiologist suspect? A. Salmonella spp. B. Escherichia coli C .Proteus spp. D. Shigella spp. 90. Which of the following tests identifies Staphylococcus aureus? A. PYR and catalase B. Catalase and coagulase C. Bile solubility and optochin 91. Which of the following is a positive catalase test? A. Fibrin clot B. Bubbles of oxygen C. Visible clumping D. Blue-green color 92. Which of the following tests is primarily done to presumptively identify Pseudomonas aeruginosa? A. Spot oxidase B. Coagulase C. PYR D. Spot indole D A A C D B B A MLT 210 Flashcards UNIT FOUR 39. 93. Which of the following is a rapid method to screen sputum specimens for the yeast Cryptococcus neoformans? A. Coagulase B. Urease C. Indole D. Oxidase 94. The microbiologist uses TSI and LIA agars to rule out stool pathogens, Salmonella and Shigella spp. The reactions observed are TSI: K/A, H2S+ and LIA: R/A. What should the technologist do next? A. Report Salmonella spp. recovered. B. Report Shigella sp. recovered. C. Report normal stool flora. D. Repeat all testing. 95. The technologist uses TSI and LIA agars to rule out stool pathogens, Salmonella and Shigella spp. The reactions observed are TSI: K/A H2S+ and LIA: K/K. What should the technologist do next? A. Report Salmonella spp. recovered. B. Report Shigella spp. recovered. C. Do further biochemical and serological tests to confirm Salmonella spp. D. Repeat all testing. 96. Which of the following media contain sodium thiosulfate and ferric ammonium citrate for the detection of hydrogen sulfide production? A .Hektoen enteric agar (HE) B. Triple sugar iron agar (TSI) C. Lysine iron agar (LIA) D. Xylose-lysine-desoxycholate agar (XLD) E. All of the above 97. A clean-catch midstream urine specimen is sent to the laboratory for culture. The results of the culture yield three organisms present, with no predominating organism. What type of workup should the technologist do? A. Complete workup of all organisms present B. No workup because of possible contamination C. Workup one of the organisms D. Workup two of the organisms 98. A positive leukocyte esterase and nitrite test (urine rapid-screening test strips) indicates: A. Most likely the patient has a UTI. B. The patient definitely does not have a UTI. C. A new specimen should be collected because these tests indicate contamination. D. The test strips are not reacting properly, and the quality control should be rechecked. B C C E B A MLT 210 Flashcards UNIT FOUR 99. If a urine culture was planted using a 0.001-mL loop and 75 colonies were observed, what is the colony-forming units per millimeter of urine (CFU/mL)? A. 75 CFU/mL B. 750 CFU/mL C. 7500 CFU/mL D. 75,000 CFU/mL 100. Rapid screening of throat specimens (nonculture method) is done to detect which of the following pathogens? A. Streptococcus pneumoniae B. Escherichia coli C. Group A ß-hemolytic streptococcus D. Staphylococcus aureus 101. Which of the following describes alpha hemolysis on sheep blood agar? A. Complete clearing around the colonies of the red blood cells in the medium B. Partial hemolysis of the red blood cells in the medium appearing as a green discoloration of the medium C. Partial hemolysis of the red blood cells in the medium appearing as a slight clearing around the colonies D. No hemolysis of the red blood cells in the medium; therefore no change in the color of the medium 102. Which of the following tests can be used to identify group A ßhemolytic streptococcus? A. PYR test B. Bacitracin disk C. Optochin D. A and B E. B and C 103. Which of the following is/are done to identify Streptococcus pneumoniae? A. Ethylhydrocupreine hydrochloride B. Optochin C. Bile solubility D. Desoxycholate E. All of the above 104. Which of the following is one cause of bacterial vaginosis and is best diagnosed by the presence of microscopic “clue cells”? A. Gardnerella vaginalis B. Neisseria gonorrhoeae C. Chlamydia trachomatis D. Trichomonas vaginalis D C B D E A MLT 210 Flashcards UNIT FOUR 105. Which of the following is an obligate intracellular pathogen that is a prevalent sexually transmitted disease? A. Gardnerella vaginalis B. Neisseria gonorrhoeae C. Chlamydia trachomatis D. Trichomonas vaginalis 106. Which of the following organisms can be routinely detected by culture and nucleic acid testing? A. Gardnerella vaginalis B. Neisseria gonorrhoeae C. Chlamydia trachomatis D.Trichomonas vaginalis E. A and C AB. B and C AC. C and D 107. A progressive syndrome with symptoms of fever, chills, low blood pressure, respiratory distress, and disseminated intravascular coagulation is called: A. Bacteremia B.Septic shock C. Transient bacteremia D. Exotoxin 108. A common gram-negative organism found in blood is: A. Staphylococcus aureus B. Escherichia coli C. Coagulase-negative staphylococci D. Streptococcus pneumoniae 109. Which of the following bacteremias can result from possible wound abscesses and are present for a time in the blood, followed by a period with no bacteria? A. Intermittent bacteremia B. Transient bacteremia C. Septicemia D. Continuous bacteremia 110. Which of the following is false about blood cultures? A. Blood/medium ratio 1:2 to 1:5 B. Blood/medium ratio 1:5 to 1:10 C. Sodium polyanethol sulfonate (anticoagulant) D. Broth medium C AB B B A A MLT 210 Flashcards UNIT FOUR 111. Automated methods of culturing blood have replaced traditional methods in most laboratories because of: A. More rapid detection time for many pathogens B. Monitoring of growth without visual inspection or subculture of the bottles C. The ability to use the report in conjunction with a computerized laboratory management information system D. All of the above 112. The lowest concentration of an antimicrobial agent that will visibly inhibit the growth of the organism being tested is called: A. Bacteriostatic B. Bactericidal C. Minimal inhibitory concentration D. Minimum bactericidal concentration 113. Which of the following is an agar disk diffusion method of testing for antimicrobial susceptibility? A.Microdilution B. Macrodilution C.Kirby-Bauer method D. Minimum bactericidal concentration 114. How do you interpret no zone of inhibition around an antibiotic disk in the disk diffusion method? A.Susceptible B.Resistant C.Intermediate D. Repeat testing; there should always be a zone of inhibition 115. A microbiologist is preparing a Mueller-Hinton agar for disk diffusion testing and has inoculated the plate with the appropriate standardized inoculum. The microbiologist is interrupted by a phone call from a physician that takes 20 minutes to track the requested information. How should the technologist proceed with the disk diffusion testing? A. Continue as planned and place the disks on the agar plate. B. Continue as planned, but add quality control testing to ensure proper results. C. Discard the plate and, with the same inoculum, swab a new Mueller-Hinton agar. D. Discard the plate and the inoculum and start anew. 116. All of the following are characteristics of fungi except: A. Cell wall made of peptidoglycan B.Hyphae C. “Fuzzy” or woolly appearance D.Eukaryotic D C C B D A MLT 210 Flashcards UNIT FOUR 117. A fungal infection that affects the deeper layers of the skin, including muscle and connective tissue, is called _____ mycosis. A. Superficial B. Cutaneous C. Subcutaneous D. Systemic 118. Which is the least desirable specimen for fungal cultures? A. Hair B. Skin C. Sterile body fluids D. Swabs 119. Which of the following organisms can be detected by India ink staining and appear as budding yeast surrounded by a large clear area (capsule)? A. Nocardia spp. B. Candida albicans C. Cryptococcus neoformans D. Candida spp. 120. Which procedure can identify Candida albicans? A. India ink stain B.Germ-tube test C. Gram stain D. Acid-fast stain 121. Which of the following are the nematodes? A. Roundworms B. Flatworms C. Tapeworms D. Flukes 122. Which of the following is the feeding and reproductive stage of protozoa and what consistency of stool would you usually find them in? A. Cysts; loose or watery stool B. Cysts; formed stool C. Trophozoites; loose or watery stool D. Trophozoites; formed stool 123. What is the recommended protocol for collection of stool specimens for ova and parasite (O&P) detection? A. Three stool samples 1 or 2 days apart, all within a 10-day period B. Two stool samples 1 or 2 days apart, all within a 5-day period C. Three stool samples 1 or 2 days apart, all within a 5-day period D. Two stool samples 1 or 2 days apart, all within a 10-day period C D C B A C A MLT 210 Flashcards UNIT FOUR 124. Which of the following specimens is appropriate for detection of Plasmodium spp.? A. Stool B. Wound C. Blood D. Urine 125. Motile trophozoites found microscopically in fresh urine or fresh genital secretions is diagnostic for which of the following parasites? A. Plasmodium spp. B. Trichomonas vaginalis C. Enterobius vermicularis D. Pneumocystis jiroveci 126. The cellophane tape method is used to detect which of the following? A. Plasmodium spp. B. Trichomonas vaginalis C. Enterobius vermicularis D. Pneumocystis jiroveci 127. What is the diagnosis from the following Gram stain of urethral discharge from a male: Many polymorphonuclear leukocytes (PMNs) Few gram-negative intracellular diplococci 128. Interpret the following case study: A 25-year-old female presented to the emergency room with vaginal discomfort. The physician ordered a hanging drop (wet prep for Trichomonas vaginalis), KOH prep, Gram stain, culture, and Gen-Probe. The results are: Hanging drop: no motile trichomonas seen, clue cells present KOH prep: no fungal elements seen Gram stain: Many gram-variable bacilli Few squamous epithelia Rare amount gram-positive bacilli Genital culture: pending Gen-Probe: negative for Neisseria gonorrhoeae and negative for Chlamydia trachomatis 129. A fundamental principle of immunology is recognition of: A. Body cells B. Molecules C. Self from non-self D. Antibodies 130. Serology is the study of: A. Antigens B. Antibodies C. Chemical reactions D. Infectious diseases E. Both A and B C B C Neisseria gonorrhoeae Gardnerella vaginalis causing bacterial vaginosis (BV) C E MLT 210 Flashcards UNIT FOUR 131. Age is a factor that influences an individual’s defense against disease. A. True B. False 132. Factors that influence an individual’s defense against disease include status of general health. A. True B. False 133. Factors that influence an individual’s defense against disease include nutritional status. A. True B. False 134. Factors that influence an individual’s defense against disease include taking mega-doses of vitamins. A. True B. False 135. The first line of body defense is: A. Unbroken skin B. Normal biota C. Body fluids (e.g., tears, saliva) D. All of the above 136. Another name for innate immunity is ____ immunity. A. Natural B. Inborn C. Acquired D. Both A and B 137. The second line of body defense consists of: A. Neutrophils, tissue basophils, macrophages B. Complement and lysozyme C. Interferon D. All of the above 138. The third line of defense is: A. Acquired B. Adaptive C. Natural D. Both A and B 139. Humoral-mediated immunity is associated with: A. T lymphocytes B. B lymphocytes C. Neutrophils D. Monocytes 140. Immediate hypersensitivity reactions are associated with: A. IgM B. IgG C. IgE D. IgD A A A B D D D D B C MLT 210 Flashcards UNIT FOUR 141. Arrange the stages in an antibody response in the correct sequence. 1 = Log phase 2 = Lag phase 3 = Plateau phase 4 = Decline A. 1, 2, 3, 4 B. 2, 3, 1, 4 C. 4, 2, 1, 3 D. 2, 1, 3, 4 142. A typical antibody response takes about ____ to develop sufficiently. A. 24 hours B. 72 hours C. 1 week D. 2 weeks 143. The antibody subclass associated with a primary response is: A. IgM B. IgG C. IgD D. IgE 144. The antibody subclass associated with a secondary response is: A. IgM B. IgG C. IgD D. IgE 145. An anamnestic response differs from a primary antibody response in several important aspects. A secondary response has a longer lag phase, a longer plateau phase, and a more gradual decline in antibody titer. A. True B. False 146. An anamnestic response differs from a primary antibody response in several important aspects. IgG-type antibodies are the principal class of antibody formed in a primary response. A. True B. False 147. An anamnestic response differs from a primary antibody response in several important aspects. In a secondary response, antibody concentrations reach a higher titer. A. True B. False 148. An anamnestic response differs from a primary antibody response in several important aspects. The plateau levels in a secondary response are typically tenfold or more than the plateau levels in the primary response. A. True B. False D D A B B B A A MLT 210 Flashcards UNIT FOUR 149. Agglutination is comprehensively defined as: A. Aggregation of soluble test antigens B. Aggregation of particulate test antigens C. The physical attachment of antibody molecules to antigens D. The establishment of cross-links between sensitized particles 150. A popular method for waived, over-the-counter testing is: A. Chemiluminescence B. Fluorescent in situ hybridization (FISH) C. Signal amplification technology D. Enzyme immunoassays 151. A convalescent blood specimen should be collected ____ after the onset of symptoms. A. 24 hours B. 72 hours C. 1 week D. 2 weeks 152. -HCG can be detected in over-the-counter tests in about ____ after a missed period. A. 2 to 3 days B. 7 days C. 8-10 days D. 12 days 153. Testing for ß-hCG is more sensitive if ____ is used as a specimen. A. Urine B. Serum C. Saliva D. Tears 154. If urine is used for pregnancy testing, it should be: A. First morning B. Random C. 24-hour D. Sterile 155. A positive reaction when using the MonoSlide rapid slide test for infectious mononucleosis indicates the presence of: A. Epstein-Barr virus B. Heterophil antibodies C. Forssman antibodies D. Guinea pig antibodies 156. The antinuclear antibody (ANA) assay is specific for systemic lupus erythematosus. A. True B. False B 157. Rheumatoid factor has been associated with IgM, IgG, and IgA immunoglobulin classes. A. True B. False A D D D B A B B MLT 210 Flashcards UNIT FOUR 158. Rheumatoid factors are autoantibodies. A. True B. False 159. In testing for rheumatoid factor, lipemic or hemolyzed specimens can cause: A. False positive results B. False negative results C. No effect on the test results A 160. In testing for rheumatoid factor, systemic lupus, syphilis or hepatitis can cause: A. False positive results B. False negative results C. No effect on the test results 161. In the RPR test for syphilis, the presence of conditions such as infectious mononucleosis can cause: A. False positive results B. False negative results C. No effect on the test results Match the term with the appropriate definition. A. Vaccination B. Infusion of plasma containing antibodies C. Obtained from colostrum D. As a result of suffering from the disease 162. Active natural immunity 163. Active artificial immunity 164. Passive natural immunity 165. Passive artificial immunity Match the leukocyte type with a specific function A. Recognition of foreign antigen and antibody production B. Phagocytosis and antigen-presentation C. Phagocytosis without antigen presentation 166. Granulocytes 167. Monocytes-macrophages 168. Lymphocytes-plasma cells Match the antibody response term with the correct description. A. No detectable antibody B. Antibody titer stabilizes C. Antibody is catabolized D. Antibody titer increases 169. Lag 170. Decline 171. Log 172. Plateau Match each statement with correct item below. A. A conjugated antibody is used to detect antigen-antibody reactions. B. Antibodies can act as antigens and react with antiimmunoglobulins. 173. Direct immunofluorescent assay (DFA) 174. Indirect immunofluorescent assay (IFA) A A A 162.D 163.A 164.C 165.B 166.C 167.B 168.A 169.A 170.C 171.D 172.B 173.A 174.B MLT 210 Flashcards UNIT FOUR