Undergraduate Course Synopsis: General Microbiology course synopsis 11:680:390; 4 credits. Two 1 hour 20 min lectures plus a mandatory 3 hour laboratory per week. The course is team taught by faculty of the Department of Biochemistry and Microbiology, SEBS. Offered: This lecture plus laboratory course is offered each Fall, Spring, and Summer. Prerequisites and Registration Restrictions: Pre-requisites: 01:119: 101-102 General Biology; 01:160:161-162 General Chemistry (4, 4) and 11:160:209 or 11:160:307 Organic Chemistry. Non-Rutgers courses offered as alternate prerequisites are individually assessed and approved depending on content. Description: This course covers basic principles of microbiology and provides an introduction to the diversity, physiology, morphology, genetics, ecology, applications and pathogenicity of microbes. STUDENT LEARNING OUTCOMES: Upon completion of the course students will Understand the structural similarities and differences among microorganisms and the unique structure/function relationships of prokaryotic cells. Know the fundamentals of microbial gene expression and regulation. Appreciate the diversity of microorganism and microbial and communities and recognize how microorganism solve the fundamental problems their environments present. Understand how microorganisms cause disease (and be able to explain why one strain is pathogenic and another is not.) In the laboratory students will Learn the proper use of a phase contrast microscope to observe microorganisms and report observed characteristics. Master aseptic technique and be able to perform routine culture handling tasks safely and effectively. Develop scientific literacy as a microbiologist. Learn to collect, interpret, and present scientific data in microbiology and related fields. Apply their knowledge of microbial structure and metabolism to the identification of unknown microorganisms. Lecture topics: Lec Topic Chap Instr 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 22 23 24 24 Introduction Microbial Cell Structure Microbial Cell Structure/Function Introduction to Viruses Nutrition and Metabolism Microbial Cell Growth Principles of Microbial Molecular biology Regulation of Gene Expression; Bacteria and Archaea Bacterial Genetics Bacterial Genetics Industrial Microbiology Microbial Evolution, Systematics and Taxonomy Prokaryotes Bacteria (Pt. 1) Bacteria - Proteobacteria Prokaryotes (part II continued) and Archaea Ecology - Introduction Ecology - Methods Ecology - Ecosystems Ecology (Cycling, Symbioses, Bioremediation) Eukaryotic microbes (Protists/Fungi) Viral Diversity Microbial Growth Control Microbial Interactions with Humans Epidemiology 1, 2 2 3 9 4 5 6(7) 8 10 11/12 15/26 16 17/18 18/19 22-25 22-25 22-25 22-25 20 21 26 27 32 DD DD DD DD CV CV CV CV CV CV CV TB TB TB TB TB TB TB DD DD DD DD DD 26 27 28 Person to Person Microbial Diseases/Vectorborne & Soilborne Pathogens Wastewater Treatment, Water Purification & Waterborne Microbial Diseases & Foodborne Microbial Diseases Food Preservation 33/34 35 36 DD DD DD Course Coordinator: Professor Diane Davis (DD), 848 932-5635 davis@aesop.rutgers.edu Professor Costa Vetriani (CV), 848.932.3379 vetriani@aesop.rutgers.edu Professor Tamar Barkay (TB), 848.932.5664 barkay@aesop.rutgers.edu Lab Coordinator: Professor Ines Rauschenbach, 848.932.5418; inesrau@aesop.rutgers.edu Assistant: Kathy Maguire, 848.932.5642 maguire@aesop.rutgers.edu Course Text: Brock’s Biology of Microorganisms. 2012. Madigan, M., J.M. Martinko, D.A. Stahl and D.P. Clark. 13th edition. [Benjamin Cummings, Boston, MA). Laboratory: Students will master the basic laboratory skills and techniques necessary to work efficiently and safely in a microbiology laboratory including the concept and use of “aseptic technique”, fundamental to practical microbiology. Students will strengthen written and oral communication skills. The laboratory exercises have been developed to stress the following areas critical for today’s microbiologist: microscopy, culture handling and maintenance, microbial biochemistry and physiology and molecular biology. Rutgers Laboratory Manual: 680:390 Laboratory Exercises is published through the Department of Biochemistry and Microbiology (2013). Purchase information is available to registered students through SAKAI. Additional laboratory Information: Contact Dr. Ines Rauschenbach via email: inesrau@aesop.rutgers.edu. Examinations: Student grades are based on three examinations (75%) and laboratory performance and assignments (25%). The theory examinations have a multiple choice plus short written answer format. The laboratory grading is based on reports and quizzes throughout the semester in combination with a practical laboratory examination. Other Requirements: The laboratory section of the course in an integral component. Successful completion of the lab is required to pass General Microbiology. Syllabus: A detailed syllabus will be available at the first class meeting and posted on the course Sakai site. Additional Information: Contact Dr. Diane Davis; davis@aesop.rutgers.edu