Maiss AbdulHadi Marafie
Date of Birth 18/10/1985
Maiss_Marafie@hotmail.com
+ (965) 99884983
Jabriya, Block 11, Street 108, House 49
EDUCATION
Kuwait University
B. Sc.
Major in Microbiology
Minor in English Translation
M. Sc.
Major in Microbilogy
GPA:
Major GPA:
Graduation Date:
GPA:
Graduation Date:
3.33
3.76
June ‘08
3.77
June ‘12
OBJECTIVE
Microbiology graduate keen for scientific research/masters/PhD and interested in
teaching biological sciences. Very eager to improve knowledge and experience in
environmental microbilogy and is willing to put forward full efforts. Have laboratory
skills in relation to biology especially microbiology and molecular biology.
RESEARCH EXPERIENCE
Hydrocarbon Utilization by Microorganisms under Halostress[2012]
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This title was my Masters degree topic in Microbiology
Samples were collected from soil, water pond, rhizosphere and phyllosphere
of the plant Halocnemum strobilaceum in Al-Sabkha area in Al-Khairan.
Samples were grown on specific nutrient media for bacterial and archaeal
isolation and enumeration.
Most abundant colonies were grown, purified for further analysis.
Genomic DNA was collected for Microbial identification by 16S rRNA.
Representative isolates were compared and phylogenetic trees were drawn.
Potential of individual microorganisms to degrade simple and complex
hydrocarbon compounds was tested on 1 molar NaCl nutrient media.
Potential of individual microorganisms and microbial consortia to
bioremediate hydrocarbon compounds at different salt concentrations was
tested.
Density Gradient Gel Electrophoresis (DGGE) was accomplished using fresh
environmental samples and 9 month incubated soil samples.
Results have shown that most microorganisms were able to biodegrade oil
compounds at different salinities where the highest degradation was between 1
molar and 2 molar salt concentration.
Oil phytoremediation potential of hypersaline coasts of the Arabian Gulf using
rhizosphere technology[2010]
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Rhizospheric and phyllospheric samples were collected from Halocnemum
strobilaceum plant from the Arabian Gulf Harbor.
Hydrocarbon utilizing bacteria were grown on specific nutrient media and
enumerated.
Molecular analysis for microbial identification was followed to collect the
genomic DNA.
16S RNA genes were amplified by PCR and subjected for sequencing
Representative isolates were compared and phylogenetic tree was drawn.
Hydrocarbon biodegradation potential for individual isolated bacterium and
microbial consortia was tested
Paper was published in, Bioresource Technology, Volume 101, Issue 15,
August 2010, Pages 5786-5792.
Potentially Pathogenic Gram Negative Bacteria in Al-Sabah area - Kuwait Bay
[January 2008]
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Random samples from Kuwait Bay were collected and tested for the presence
of pathogenic Gram Negative bacteria and their ability of surviving within the
area.
The experiment was accomplished by bacterial enumeration and identification
using molecular and microbiological tests.
Analysis revealed the presence of a number of Gram Negative pathogenic
bacteria capable of surviving in Kuwait Bay for a period of time.
Presented work to Biological Sciences faculty members and participated in the
Scientific Poster Day contest.
SKILLS
Practical Laboratory Skills
 Sampling
 Media Preparation
 DNA Extraction
 Gel Electrophoresis
 Sequencing PCR
 DGGE
 Cloning
Computer Skills
 Microsoft Word
 Microsoft PowerPoint
 Currently taking a
course in ICDL
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TLC (Thin Layer Chromatography)
GLC (Gas Liquid Chromatography)
PCR (Polymerase Chain Reaction)
Respirometer
Antibiotic Sensitivity Tests
API Tests
Bioscreening
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Microsoft Excel
Mini Tab
Teaching Skills
 Participated in teaching Introduction to Microbiology
(105) during my M.Sc. studies
Languages
 English
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Arabic
AWARDS
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First Prize Winner for Best Poster and Best Performance in the “7th
Scientific Poster Day” competition [2008]
Certificate of Honor [2008]
Certificate of Honor [2007]
Certificate of Honor [2006]
INTERESTS
Travelling: Interested in exploring new cultures and new places around the world
Crocheting: Sell my crochet work for charity funds
Reading