Bacteria and Archaea KEY CONCEPT Bacteria and archaea are both single-celled prokaryotes. Bacteria and Archaea Bacteria and Archaea Prokaryotes are widespread on Earth. • Prokaryotes can be grouped by their need for oxygen. – obligate anaerobes are poisoned by oxygen – obligate aerobes need oxygen – facultative aerobes can live with or without oxygen Bacteria and Archaea • Bacteria commonly come in three forms. – rod-shaped, called bacilli – spiral, called spirilla or spirochetes – spherical, called cocci Lactobacilli: rod-shaped Spirochaeta: spiral Enterococci: spherical Bacteria and Archaea • Bacterial Cell Structure. – No nucleus – Chromosomal DNA plasma – Plasmid (circular DNA membrance with only 3 or chromosome 4 genes) cell wall – Flagellum plasmid – Pili – Cell Wall This diagram shows the typical structure of a prokaryote. Archaea and bacteria look very similar, although they have important molecular differences. pili flagellum Bacteria and Archaea • Gram Positive and Negative is based on the composition of the cell wall. – Gram Positive have 1 cell wall – Gram Negative have 2 cell walls with less peptidoglycan GRAM NEGATIVE GRAM POSITIVE Bacteria and Archaea • Gram staining identifies bacteria. - gram-positive stains purple (antibiotics work) - gram-negative stains pink (resistant to many antibiotics) Gram-negative bacteria have a thin layer of peptidoglycan and stain red. Gram-positive bacteria have a thicker peptidoglycan layer and stain purple. Bacteria and Archaea Bacteria Reproduce through Binary Fission • Binary fission is like mitosis – One cell creates 2 identical daughter cells – Can occur once every 20 minutes Bacteria and Archaea Bacteria have various strategies for survival. • Prokaryotes exchange genes during conjugation. – Most of the time, plasmids move through the conjugation bridge. conjugation bridge TEM; magnification 6000x Bacteria and Archaea Bacteria can evolve resistance to antibiotics by mutation, and then pass it to others through conjugation. A bacterium carries genes for antibiotic resistance on a plasmid. A copy of the plasmid is transferred through conjugation. Resistance is quickly spread through many bacteria. Bacteria and Archaea Bacteria may survive by forming endospores. • Endospores are thick cell walls that protect bacteria in harsh environments. – The cell goes dormant • When conditions become favorable, the endospore dissolves and the cell wakes up. Bacteria and Archaea Prokaryotes provide nutrients to humans and other animals. – make vitamins – break down food Bacteria and Archaea • Bacteria help ferment many foods. – yogurt, cheese – pickles, sauerkraut – soy sauce, vinegar Bacteria and Archaea Prokaryotes play important roles in ecosystems. – photosynthesize – recycle carbon, nitrogen, hydrogen, sulfur – fix nitrogen – Clean oil spills – Break down trash Bacteria and Archaea Some bacteria cause disease. • by invading and consuming tissues or making toxins. • A toxin is a poison released by an organism to slow an immune response. Bacteria and Archaea Bacteria and Archaea Bacteria and Archaea Antibiotics are used to fight bacterial disease. • Antibiotics destroy cell walls. • Other antibiotics destroy the ribosomes of bacteria • Many vaccines are also effective against bacterial infections • Antibiotics do not work on viruses. Bacteria and Archaea Archaebacteria vs. Eubacteria • Believed to be the oldest life forms on the planet • Are very different genetically from regular Eubacteria (normal bacteria) • Are found living in extreme environments – Acidic Hot Springs – Under Sea Volcanic Vents – High Salt Water Areas – Methanogens live in the gut of animals