Retrogradation behavior of some wheat starches and relation to staling of bread The retrogradation of amylopectin is the main factor responsible for the staling of bread, e.g. the increase in firmness during storage of bread. However, studies are appearing where it is shown that the use of a waxy wheat starch improves the shelf life of bread. The question is why would a waxy starch reduce staling of bread? As waxy starches contain almost only amylopectin, and no amylose, it would be expected that these starches should retrograde more than the normal starch with around 75% amylopectin and 25% amylose. There might be several explanations for the influence on bread staling. There might be differences in the water holding capacity between the starches, something that might influence moisture relations during storage. The retrogradation behavior might also differ between starches differing in amylopectin content, and such differences could result in a different development of firmness. In this project the retrogradation behavior of some different starches will be investigated. Normal wheat starch, waxy wheat starch, durum wheat starch and waxy durum wheat starch will be included in the study. The project will start with a literature study to summarize what is found in literature about retrogradation and bread staling for waxy starches. Starch retrogradation will be followed using differential scanning calorimetry (DSC), and retrogradation will be studied as a function of, for example, time, water content, and temperature. DSC can also be used to study the amount of unfreezeable water during storage. Texture analyzer will be used to follow the development of firmness during storage. Baking experiments might also be performed. The project is performed in collaboration with Colin Jenner, School of Agriculture and Wine, Adelaide University, South Australia Supervisors: Ann-Charlotte Eliasson, ann-charlotte.eliasson@food.lth.se and Jeanette Purhagen, jeanette.purhagen@food.lth.se Division of Food Technology, Lund University 2008-02-13