Blood Tube Guide
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DIRECTORATE of LABORATORY MEDICINE Blood Tube Guide April 2012 DLM Client Services 276 4062/4428 Clinical Biochemistry Lab 276 4375 Specialist Lab 276 4699 Duty Biochemist; Bleep 4375 Paediatric Core Laboratory 701 2233 Paediatric Duty Biochemist; 701 2255 Haematology Coag, Counts, Red cell 276 4030 Molecular Diagnostics 276 4809 Transfusion 276 4400 Immunology 276 6440 Microbiology 276 8788/8854/3961 Virology 276 8788/8854 Other Useful Numbers: Molecular Genetics Laboratory 276 -6122 Cytogenetics 76 6737 Transplantation Laboratory 276-6397 You can also visit DLM website at: www.cmft.nhs.uk/info-for-health-professionals/laboratory-medicine.aspx DIRECTORATE of LABORATORY MEDICINE ABBR EV ACTH TEST DEPARTMENT Biochemistry TUBE TOP COLOUR FOR ADULTS Green IN ICE CONTACT LAB APTT Activated partial thromboplastin time Haematology Light blue ALT Alanine transaminase Biochemistry Yellow Albumin Biochemistry Yellow ALP Aldosterone Alkaline phosphatase Biochemistry Biochemistry Green – urgent to lab Yellow Biochemistry Yellow or red AFP Alkaline phosphatase isoenzymes Alphafetoprotein Biochemistry Yellow Alpha-1 Antitrypsin Amiodarone Ammonia Biochemistry Biochemistry Biochemistry Amitriptyline Amylase Biochemistry Biochemistry Yellow Yellow Purple IN ICE. CONTACT LAB*. Yellow Yellow Amylase isoenzymes Biochemistry Yellow or red Androstenedione Angeotensin converting enzyme Biochemistry Biochemistry Yellow Yellow Anion-Gap Biochemistry Yellow Antibody to ENA Immunology Yellow Anticoagulant Control Haematology Light blue Anti-nuclear antibody Immunology Yellow Antioxidant profile Biochemistry Antithrombin Haematology CONTACT LAB BEFORE COLLECTION Light blue AP100 AP100 Immunology APO-E genoty pe AST Apolipoprotein-E genotype Biochemistry Red – URGENT TO LAB Purple Aspartate transaminase Biochemistry Yellow BCR-ABL 4 x Purple BHCG (Tumour Marker) Molecular Diagnostics Centre (Haematology) Biochemistry Bicarbonate Biochemistry Yellow Bile acid Biochemistry Yellow B12 Haematology Red ACE ENA ANA Yellow TUBE TOP COLOUR FOR PAEDIATRIC / NEONATAL Orange 4.0 ml top Orange 1.3ml or Green Neonatal 0.8ml Orange 1.3ml or Green Neonatal 0.8ml White Plain Clotted 2.0ml Orange 1.3ml or Green Neonatal 0.8ml Orange 1.3ml or Green Neonatal 0.8ml Orange 1.3ml or Green Neonatal 0.8ml White Plain Clotted 2.0ml White Plain Clotted 2.0ml Red top 1.3ml or EDTA White Plain Clotted 2.0ml Orange 1.3ml or Green Neonatal 0.8ml Orange 1.3ml or Green Neonatal 0.8ml White Plain Clotted 2.0ml White Plain Clotted 2.0ml Orange 1.3ml or Green Neonatal 0.8ml Orange 1.3ml or Green Neonatal 0.8ml Orange 1.3ml or Green Neonatal 0.8ml Orange 1.3ml or Green Neonatal 0.8ml Serum Z/1.3 bottle/white plain 2.0ml plastic Clotted DIRECTORATE of LABORATORY MEDICINE Bilirubin Biochemistry Yellow Blood count Haematology Purple Haematology Pink Red Top Biochemistry Biochemistry Purple Yellow Yellow White Plain Clotted 2.0ml C3 Blood grouping+Antibody screen Bone Markers CTX ,PINP Ca-125,Ca19-9,Ca15-3,CEA (tumour markers) C3 Immunology Yellow C4 C4 Immunology Yellow CRP C Reactive protein Biochemistry Yellow Caeruloplasmin Biochemistry Yellow Calcitonin Biochemistry Calcium Biochemistry Yellow – URGENT TO LAB ON ICE Yellow Carbamazepine Biochemistry Yellow Carbohydrate deficient transferrin CD4 Biochemistry Yellow Immunology Purple Cell Markers Immunology Purple CH100 Immunology Chloride Biochemistry Red – URGENT TO LAB Yellow Cholesterol Biochemistry Yellow Cholinesterase Biochemistry Yellow Chromagranin A and B Biochemistry Purple Chromosome Analysis Cytogenetics CIH inhibitor Immunology Clomipramine Biochemistry Light blue URGENT TO LAB Yellow Coagulation disorders Haematology Light Blue Coagulation screen Haematology Light Blue Cold agglutinins screen Haematology Pink + purple at 37’C By arrangement olny Copper Biochemistry Navy blue C -peptide Biochemistry Cord blood investigations Haematology Cortisol Biochemistry Yellow – URGENT TO LAB ON ICE 1 pink (maternal) 1 pink (cord) Yellow White Plastic Plain Clotted 5.0ml, usually ordered with caeruloplasmin unless for nutrition monitoring Orange 1.3ml or Green Neonatal 0.8ml C-reactive protein Biochemistry Yellow Creatinine Biochemistry Yellow Creatine kinase Biochemistry Yellow Cryoglobulins Immunology Red CONTACT LAB ext 6440 CD4 CRP CK Orange 1.3ml or Green Neonatal 0.8ml Orange 1.3ml or Green Neonatal 0.8ml White Plastic Plain Clotted 5.0ml, always ordered with copper White Plain Clotted 2.0ml Orange 1.3ml or Green Neonatal 0.8ml White Plain Clotted 2.0ml Orange 1.3ml or Green Neonatal 0.8ml Orange 1.3ml or Green Neonatal 0.8ml White Plain Clotted 2.0ml Orange 1.3ml or Green Neonatal 0.8ml Orange 1.3ml or Green Neonatal 0.8ml Orange 1.3ml or Green Neonatal 0.8ml Orange 1.3ml or Green Neonatal 0.8ml DIRECTORATE of LABORATORY MEDICINE CTX Crosslaps (bone marker) Biochemistry Purple Cyclosporin Biochemistry Purple D-dimer Haematology Light blue 11-deoxycortisol Biochemistry DHEA sulphate Biochemistry Yellow – KEEP ON ICE URGENT Yellow DIC screen Haematology Light blue Differential leucocyte count Haematology Purple Digoxin Biochemistry Yellow White Plain Clotted 2.0ml Direct Antibody Test (DAT) Haematology Pink Red Top DNA studies Purple EDTA Yellow Purple EDTA Dothiepin Molecular Genetics Laboratory Biochemistry Down's Syndrome Screen Biochemistry Yellow U&E Electrolytes Biochemistry Yellow EP Electrophoresis Immunology Yellow EOP Erythropotin Haematology Red ESR Erythrocyte sedimentation rate Haematology Ethanol Biochemistry Purple Can be performed from blood count sample Grey or yellow Ethosuximide Biochemistry Yellow Ethylene glycol Biochemistry Yellow or Grey Factor V Leiden (& prothrombin) genotyping Factor VIII Molecular Diagnostics Centre (Haematology) Haematology Purple Factor assay (pre/post dose) Haematology Light blue Ferritin Haematology Red FDP FSH FBC GGT Purple top White Plain Clotted 2.0ml White Plain Clotted 2.0ml Orange 1.3ml or Green Neonatal 0.8ml Serum Z/1.3 plastic bottle/white plain Clotted 2.0ml Purple/ black top. Can be performed from blood count sample Orange 1.3ml or Green Neonatal 0.8ml Orange 1.3ml or Green Neonatal 0.8ml OR White Plain Clotted 2.0ml Orange 1.3ml or Green Neonatal 0.8ml OR White Plain Clotted 2.0ml Light blue Fibrinogen estimation Haematology Light blue Fibrin degradation products (D-dimers) FK506 - Tacrolimus Haematology Light blue Biochemistry Purple Flecainide Biochemistry Yellow Folate (serum only) Haematology Red Folate ( RBC) Follicle Stim. Hormone Haematology Biochemistry purple Yellow free T4 Biochemistry Yellow Full Blood Count (FBC) Haematology Purple G6PD Haematology Purple Gamma-GT Biochemistry Yellow Serum Z/1.3 bottle/white plain 2.0ml plastic Clotted Purple top Serum Z/1.3 plastic bottle/white plain Clotted 2.0ml purple Orange 1.3ml or Green Neonatal 0.8ml Orange 1.3ml or Green Neonatal 0.8ml Orange 1.3ml or DIRECTORATE of LABORATORY MEDICINE Green Neonatal 0.8ml Genetic leuk 4 x Purple Gentamicin Molecular Diagnostics Centre (Haematology) Biochemistry GHRH Biochemistry Red GIFN Immunology Glandular fever Haematology Special 3 bottles sent with patient Purple Glucose Biochemistry Grey Yellow top 1.0ml GTT Glucose Tolerance Test Biochemistry Grey Yellow top 1.0ml HbA1c Glycated Haemoglobin Biochemistry Purple Purple top Growth Hormone Biochemistry Yellow White Plain Clotted 2.0ml Gut hormone Biochemistry Purple top on ice Haematinics Haematology Bottle from lab KEEP ON ICE – URGENT* Red + purple Haematocrit Haematology Purple Haemochromatosis genotyping Haemoglobin Molecular Diagnostics Centre (Haematology) Haematology Light blue or purple Haemoglobin F Haematology Purple Haemoglobin A2 Haematology Purple Haemoglobinopathy Haematology 2 x 5ml Purple purple HbA1c Biochemistry Purple Purple top Haemolytics Haematology purple purple Molecular Diagnostics Centre (Haematology) Haematology 4 x 5 ml Purple Haptoglobin Haematology Red Human chorionic gonadotropin Heparin Biochemistry Yellow Haematology Light blue HLA B27 Transplantation Lab Purple (EDTA tube) Purple (EDTA tube) HLA B57 typing for Abacavir hypersensitivity HLA antibodies renal recipient Transplantation Lab Purple (EDTA tube) Purple (EDTA tube) Transplantation Lab HLA typing HSCT patient Transplantation Lab Red Top tube (Plain). No anticoagulant Purple (EDTA tube) Red/White Top tube (Plain) No anticoagulant. Purple (EDTA tube) Homocysteine Biochemistry Orange 1.3ml or Green Neonatal 0.8ml Biochemistry Grey NOTE TIME ON BOTTLE and send immediately to lab. for rapid separation. Purple or yellow Biochemistry Yellow Immunoglobulins: Immunology Yellow IgA/IgG/IgM Immunology Yellow IgE – allergen specific Immunology Yellow IgE – total Immunology Yellow IGF1 ( Insulin like growth factor 1) Biochemistry Red HFE Haemophilia genetics mutation screen Hams test HCG Hydroxypcortisol cortisol) Imipramine IGF1 or (17-OH Red top Orange 1.3ml or Green Neonatal 0.8ml Purple Purple Orange 1.3ml or Green Neonatal 0.8ml DIRECTORATE of LABORATORY MEDICINE IgG/IgM antibody to ds-DNA ID INR LH MCT MCHC MCV Immunology Yellow IgG/IgM anti-cardiolipin Immunology Yellow IgG1/IgG2/IgG3/IgG4 subclass Immunodeficiency Immunology Yellow Immunology Purple Immunofixation Immunology Yellow Immunophenotyping Immunology Purple Insulin Biochemistry Red- ON ICE + grey Intrinsic factor antibodies Haematology Red INR Haematology Light blue Iron Saturation Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml Iron Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml Isoenzymes (Alkaline phosphatase/amylase isoenymes) Biochemistry Yellow or red Orange 1.3ml or Green Neonatal 0.8ml OR White Plain Clotted 2.0ml Karotyping (chromosome analysis) KCT Molecular Genetics Laboratory Haematology Green (lithium/Heparin) tube Light Blue Kleihauer test Haematology Pink Lactate Biochemistry Yellow top 1.0ml Lamotrigine Biochemistry Glucose tube (grey) SEND STRAIGHT TO LABORATORY Yellow Lead levels Biochemistry Purple Luteinising Hormone Biochemistry Light green Red top 1.3ml PLASTIC EDTA Orange 1.3ml or Green Neonatal 0.8ml Lipids Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml Lithium Biochemistry Yellow White Plain Clotted 2.0ml Long chain fatty acids Biochemistry Purple NOTE: this is not the same test as free fatty acids Lupus anticoagulant Haematology 2 x Light blue Lymphocyte subset Immunology Purple Magnesium Biochemistry Yellow Malarial parasites Haematology Purple Mast cell tryptase Immunology MCH Haematology Yellow NOTE TIME ON BOTTLES AND REQUEST CARD Purple Mean cell haemoglobin concentration Mean cell volume (MCV) Haematology Purple Haematology Purple Orange 1.3ml or Green Neonatal 0.8ml ON ICE White Plain Clotted 2.0ml Orange 1.3ml or Green Neonatal 0.8ml DIRECTORATE of LABORATORY MEDICINE Mercury levels Biochemistry Purple Methotrexate Biochemistry Yellow Mitochrondial DNA Monospot (Paul Bunnell) Molecular Genetics Laboratory Haematology Purple Neural tube defect screen Biochemistry Yellow Oestradiol Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml Orosomucoid Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml Osmolality Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml Procollogen type 1 (bone marker) Procollogen type 3 (liver fibrosis marker) Paul Bunnell test (Monospot) Biochemistry Yellow Biochemistry Yellow Haematology Purple Paracetamol Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml PTH Parathyroid hormone Biochemistry Purple Purple top PTHrp PTH related peptide Biochemistry Purple top PCR/ viral load Virology Contact lab for special tube Purple EDTA Phenobarbitone Biochemistry Yellow Phenytoin Biochemistry Yellow Phosphate Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml OR White Plain Clotted 2.0ml Orange 1.3ml or Green Neonatal 0.8ml OR White Plain Clotted 2.0ml Orange 1.3ml or Green Neonatal 0.8ml Plasma Metadrenalines Biochemistry Plasma osmolality Biochemistry Purple – URGENT TO LAB IMMEDIATELY Yellow Plasma renin Biochemistry Plasma viscosity Haematology green sent immediately to lab 4 x purple Platelet aggregation / function Haematology 6 x light blue (By appt) Platelet aggregation/function + nucleotides Porphyrins Haematology 8 x light blue (By appt) Biochemistry Purple top + urine. Contact lab. Before collection and send straight to lab. Protecting samples from light. Potassium Biochemistry Purple top + urine. Contact lab. Before collection and send straight to lab. Protecting samples from light. Yellow Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml PINP PIIINP/ P3NP Pregnancy testing (blood) White Plain Clotted 2.0ml Orange 1.3ml or Green Neonatal 0.8ml Purple top tube Orange 1.3ml or Green Neonatal 0.8ml DIRECTORATE of LABORATORY MEDICINE PSA, FPSA Progesterone Biochemistry Yellow White Plain Clotted 2.0ml Prolactin Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml Propranolol Biochemistry Yellow Prostate Specific Antigen ( Free or Total) Protein S (free) Biochemistry Yellow Haematology 2 x Light blue Protein C Haematology 2x Light blue Protein - total Biochemistry Yellow Prothrombin time Haematology Light blue Molecular Diagnostics Centre (Haematology) Immunology 1 x Purple Quantitative HCG (For Blood pregnancy test / ectopic pregnancy) Renin Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml Biochemistry Purple Reticulocytes count Haematology Rheumatoid factor Immunology dark green send immediately to lab (NOT on ice) Purple Can be performed from blood count sample Yellow Salicylate Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml Selenium Pancreatic Laboratory Red White Plastic Plain Clotted 5.0ml Serology bacteria / viral Virology Red Serum and red cell folate Haematology Purple + red SHBG Biochemistry Yellow Sickle test Haematology Purple Prothrombin (& Factor Leiden) genotyping Quantiferon QHCG RF V Orange 1.3ml or Green Neonatal 0.8ml Contact LAB Serum Z/1.3 plastic bottle/white plain Clotted 2.0ml and purple top. White Plain Clotted 2.0ml Sirolimus Biochemistry Purple Purple top Sodium Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml Tacrolimus Biochemistry Purple Purple top Testosterone Biochemistry Yellow White Plain Clotted 2.0ml Thalassaemia Haematology Purple Theophylline Biochemistry Yellow Haematology 2 x Light Blue Thyroglobulins Biochemistry Yellow TFT, T3, T4, TSH Thyroid Function Test Biochemistry Yellow TPO Thyroid peroxidase Immunology Yellow TIBC Total Iron Binding Capacity Biochemistry Transferrin saturation Biochemistry Not available for adults, see transferrin saturation Yellow FK506 Thrombophilia screening Orange 1.3ml or Green Neonatal 0.8ml Orange 1.3ml or Green Neonatal 0.8ml OR White Plain Clotted 2.0ml Orange 1.3ml or Green Neonatal 0.8ml DIRECTORATE of LABORATORY MEDICINE TropT Transferrin Biochemistry Yellow Triglycerides Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml Troponin T Biochemistry Yellow White Plain Clotted 2.0ml Tumour markers CA 15- 3,CA 19–9,CA 125, CEA Urate Biochemistry Yellow White Plain Clotted 2.0ml Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml Urea Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml Uric Acid Biochemistry Yellow Orange 1.3ml or Green Neonatal 0.8ml Valproic Acid/Valproate Biochemistry Yellow White Plain Clotted 2.0ml Virology Serology Virology Red Virology – PCR/viral load Virology Purple Vitamin B12 Haematology Red Vitamin C 2 x green Vitamin D Biochemistry 64067 prior to collection to alert lab to collect samples from OPD Biochemistry Yellow White Plain Clotted 2.0ml Vitamin E Biochemistry Yellow or red White Plain Clotted 2.0ml Von Willebrand Haematology 2 x light blue Von Willebrand genetics or mutation screen Zinc Molecular Diagnostics Centre (Haematology) Biochemistry 4 x light blue Navy blue White Plastic Plain Clotted 5.0ml DIRECTORATE of LABORATORY MEDICINE Coagulation Specimen Requirement Guide Coagulation Test Blue Top 4.5ml Number of Specimens COAG Profile DIC Screen (includes Fibrinogen and D-Dimer/FDP) D-DIMER (FDP) Any combination of these tests can be performed on 1 sample HEPARIN Control INR DLE (Lupus Screen) THROMBOPHILIA Screen (includes Anti-thrombin 3 AT111, Protein C, Protein S, Factor V Leiden F5L, Prothrombin Gene Variant PGV) FACTOR Assays (multiple) Von Willebrand Screen (includes Factor 8 assay F8C, Ristocetin Co Factor RCF, Von Willebrand antigen VWAG. Platelet Aggregation/Nucleotides For any queries contact the Coagulation lab. On ext 12123. 2 2 1 2 By appointment only, please check with the coagulation laboratory. Ext 12123.
