Course Outline
BIOCHEMISTRY 4200 (Proteins and Nucleic Acids)
Spring Semester 2005
Lecture: Tu, Th 16:30 h – 17:45 h D633
Lab:
M
13:00 h – 15:30 h E770
Department of Chemistry and Biochemistry
University of Lethbridge
Lecturer:
Hans-Joachim Wieden
E868 UH
Office Hours:
hj.wieden@uleth.ca
Ph: 329 2303
M 13:00 h – 15:00 h
W 9:00 h – 11:00 h or by appointment
Lab Instructor
Michelle Duke
E856 UH
duke@uleth.ca
Ph: 329 2088
Textbook:
Lehninger Principles of Biochemistry, 4th Ed. 2004
By D.L. Nelson and M. M. Cox
Prerequisits:
Chemistry 2410, 2710, Biochemistry 3020
General Information Biochemistry 4200 will deal with the biochemistry of proteins and nucleic
acids including protein-ligand and protein-protein interactions; membrane proteins, enzyme
mechanisms, and modification of gene expression and rapid kinetics. Laboratory projects will
involve the purification and characterization of enzymes and DNA.
Reference material will consist of textbook chapters, selected research articles, and specific
internet resources. Reference material will be posted weekly. Copies of all reference material will
be made available.
The midterm exams will deal with material covered in the lectures prior to the exam. The final
exam will be comprehensive, but there will be an emphasis on the more recent material.
Lecture Outline
The course will be separated into three sections of roughly equal size.
For each section, a topic will be introduced, several example systems will be studied and
underlying methodologies will be discussed.
I.
Biophysical Methods
Modern research tools and techniques for studying macromolecular function.
II.
Macromolecular Interactions
Structure and function of representative enzymes.
III.
Omics
Genomic, proteomics, metabonomics, and others.
Laboratory Outline
Will be provided or discussed in lab
Course Outline
Grade Composition:
Midterm Exams
50min
50min
15%
15%
Feb 3rd
Mar 10th
Final Exam
180min
40%
Date set by registrar
Laboratory
(tentative date, 2 weeks notice)
(tentative date, 2 weeks notice)
30%
TOTAL
100%
Please Note : Failure to attend a Midterm exam will normally result in a score of zero, unless you
have a good reason for being absent (Instructors discretion). In the case of illness, a medical
certificate must be presented. In either case, the final exam will be pro-rated to reflect the missed
Midterm exam. A missed final exam will normally result in a score of zero. Only the Dean (or
Associate Dean) of Arts & Science can authorize a "make-up" final exam.
A passing grade must be achieved in both the lecture and laboratory component of the
course in order to obtain credit.