Antibodies and Related Reagents for Signal Transduction Research
DNMT
MeCP2
HMT
HP1
HDAC
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Epigenetics
Percent Input
C
1.6
1.4
1.2
1.0
0.8
0.6
0.4
0.2
0
Ezh2 (D2C9) XP®
Rabbit mAb #5246
Normal Rabbit
IgG #2729
HoxA2
α Satellite
Ezh2 (D2C9) XP Rabbit mAb #5246:
IHC analysis of paraffin-embedded human lymphoma
(A) using #5246. Confocal IF analysis of HeLa cells
(B) using #5246 (green) and S6 Ribosomal Protein
(54D2) Mouse mAb #2317 (blue). Actin filaments
were labeled with DY-554 phalloidin (red). Chromatin
immunoprecipitations (C) were performed with crosslinked chromatin from 4 x 106 NCCIT cells and either
5 µl of #5246 or 2 µl of Normal Rabbit IgG #2729
using SimpleChIP® Enzymatic Chromatin IP Kit
(Magnetic Beads) #9003. The enriched DNA was
quantified by real-time PCR.
®
B
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TOP IMAGE: Heterochromatin is characterized by a repressive, tight packaging of nucleosomes, which
impedes transcription factors from gaining access to regulatory sites on the DNA. Methylation of cytosine
bases in regions called CpG islands, found in many gene promoters, leads to formation of transcriptionally repressed heterochromatin. Methylation of cytosine bases by DNA methyltransferases (DNMTs)
facilitates recruitment of Methyl-CpG-binding Protein 2 (MeCP2), which brings along other associated
proteins, including histone deacetylases (HDACs), histone methyltransferases (HMTs), and Heterochromatin Protein 1 (HP1). These proteins then facilitate the deacetylation and methylation of histone
proteins, resulting in the formation and maintenance of the repressive state of heterochromatin. To view
our epigenetics digital animation movie and for more information, please visit www.cellsignal.com.
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The highest quality antibodies for the study of