Mark S. Nardone
FAES/NIH
Director, Bio-Trac Program
301-496-8290 nardonem@mail.nih.gov
One Cloister Court, Bldg 60, Rm 60
Bethesda, MD 20814-1460

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Hands-on Training Workshops in Targeted Areas of
Molecular Biology
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Taught by over 250 Active Researchers/Bench Scientists
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Presented at a Graduate/Post-Graduate Level
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Open to the Scientific Community
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Average 3-5 Days in Length
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Currently 56 Different Workshops Offered
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Specialty Programs

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NIH Researchers, Post Docs, Post Bacs, Fellows
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Lab Chiefs, Principle Investigators, Lab Technicians
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FDA, USDA, USUHS, WRAIR, USAMRIID, CDC,
US Patent Office, NIST
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Academia
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Researchers, Teachers, Graduate & Undergraduate
Students
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Private Industry
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Over 16,000 Scientists Have Participated in a Bio-Trac
Program
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Presidents & V.P’s of Pharmaceuticals; Lab Chiefs
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Bench Scientists
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Technicians

Where Are Bio-Trac
Attendees Coming From

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Effective Use of Time and Resources
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Stay on Top of the Latest Developments in
Your Area of Research
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Your Research Has Taken You into a New Field of Study
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Career Advancement
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Budget Cuts – Having to do More With Less

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Formally Established in the Fall of 1985
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Evolved Out of a Series of EEO Training programs in the late 70s
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Roland M. Nardone, Ph.D. – CUA
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Tissue Culture
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Cell Line Authentication
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Drs. Lou Cohen, Earl Laurence and Freddie
Brown (NIDDK)

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Immunofluorescence and Confocal Microscopy
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Digital Imaging in Microscopy

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Transgenic Technology

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Laser Capture Microdissection

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Cellular Immunology
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Immunocytochemistry
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Immunochemistry and Monoclonal Antibody
Production
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Vaccines: Development and Evaluation of Efficacy

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Proteomics: Principles and Methods
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Clinical Proteomics and Biomarker Discovery
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Genome Analysis
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Protein-DNA Interactions: Gene Specific and
Epigenetic Regulation of Expression of the
Mammalian Genome
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Protein Informatics
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Epigenetics
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Next Generation Sequencing
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RNA Seq

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DNA Micro Arrays: Fabrication and
Applications
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Advanced Microarray Analysis and Pathway
Integration
GeneChip ® CustomSeq™ Arrays

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Animal and Human Cell Culture: Methods and
Applications
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Cryobiology: Principles and Methods
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Cell Line Identification and Authentication
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Stem Cells

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Collaboration with the Center for
Regenerative Medicine Branch (CRM) at the
NIH
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Stem Cells: Principles and Methods
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Human Induced Pluripotent
Stem Cells (hiPSC); Differentiation to Neural
Lineages
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Engineering with CRISPR, TALENs, and ZNFs
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Making iPSCs From Blood
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Making Cardiomyocytes from iPSCs
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Stem Cell Industry Symposium

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PCR Methodology: Principles, Optimization and Applications
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Real Time and Quantitative PCR

Expression Analysis: siRNA & miRNA
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Small Interfering RNA (siRNA) and Functional
Genomics
• miRNA: Tools and Technologies for the
Quantitative and Functional Analysis in
Mammalian Cells
In situ analysis of miRNA expression

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NIH Institutes (NCI, NIEHS, NINR, NIDDK, NINDS)
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USDA
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FDA
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US Patent Office
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Merk, UpJohn, Eli Lilly, Glaxo SmithKline,
Boehringer Mannheim….
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Colleges

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Custom Designed Training Workshops
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Provide Training at Your Facility
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Save Time, Travel and Money
Next Gen Sequencing Workshop at Colorado State University
March 2014

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Lab Safety Group
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NCI Cancer Prevention Workshop
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NIAMS – Stem Cell Workshops - Center for
Regenerative Medicine (NCRM)
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Stemgent
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StemCell

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Transgenic Core Facility
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Angiogenesis Core Facility
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Microarray Core Facility
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Confocal Core Facility
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LCM Core Facility

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Zeiss – Immunofluorescent Microscopes
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Roche, ABI, Stratagene – Real Time PCR
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BD - Flowcytometer
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MMI, Zeiss Palm, Leica, MDS Arcturus – Laser
Capture Microdissection
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Nikon, Zeiss – Microinjection Scopes

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Minority Faculty Student Program
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Provides biotechnology training to minority students and faculty members
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Participants from Historically Black Colleges and Universities, Hispanic Serving Institutes and Indian Tribal Colleges or Universities
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Since the inception of the program in 1992, there have been 37 different MFSP workshops
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MFSP has provided over 480 students and 425 faculty members and touched over 90 institutions
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Participants gain insights and experience in relevant specialized areas of biotechnology through hands-on lab work and lectures
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Attendees also acquire information pertaining to research training opportunities at the NIH along with intramural and extramural funding opportunities

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Collaboration with the National Institute of Nursing Research
(NINR)
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One month intensive research hands-on training program to increase the research capability among graduate students and faculty
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Helps develop and expand the basis for clinical practice in genetics among clinicians
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Established in 2000 and has provided training to more than
230 nurses
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130+ peer reviewed publications have come from SGI
Participants
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47 SGI graduates have received Federal funding for the research proposal they developed during the SGI program

Junior Scientist Training Program
Established Fall 2010
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A quality training program at the NIH that bridges the gap between the laboratory skills acquired by biology graduates to the proficiency level necessary to be productive in a research laboratory setting
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9 Month Program
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16 Laboratory Sessions
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23 Lecture Presentations
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6 Graduate Credits through the FAES

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NIH Post Bacs, Post Docs, Fellows, Research
Scientists
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Strengthen laboratory skills
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Strengthen effectiveness as a researcher
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Recent College Graduates, 4 th Year Biology Major
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Strengthen graduate school application
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Strengthen skill set for employment in the sciences
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Strengthen internship application

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Acceptance into Graduate School
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Acceptance into Internship Programs
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Employment in the Sciences

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Expansion of JSTP in the USM System
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New Courses
– iPSC in Cardiomyocytes
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RNA-Seq
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Super Resolution Microscopy
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New Laboratory Facilities (2016-17)
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Two New Training Laboratories in Building 10
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Research Training for Medical Students
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Working with Area Medical Schools
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Collaborations With Area Institutions