17. Punnett square 18. test cross 19. probability 20. pedigree 21. sex-linked trait 22. polygenic trait 23. incomplete dominance 24. codominance 25. multiple alleles CHAPTER 9 DNA: The Genetic Material ACROSS 2. DEOXYRIBOSE 4. POLYMERASE 5. VACCINE 6. TRANSFORMATION 8. HELIX 10. BACTERIOPHAGE 11. HELICASE 12. NUCLEOTIDE DOWN 1. COMPLEMENTARY 3. PAIRING S. VIRULENT 7. REPLICATION 9. FORK CHAPTER 10 How Proteins Are Made ACROSS 2. LAC 3. RIBONUCLEIC 4. POLYMERASE 5. MESSENGER 6. GENE 8. TRANSCRIPTION 12. CODON 14. OPERATOR 16. INTRON DOWN 1. URACIL 3. REPRESSOR 7. EXON 8. TRANSLATION 9. ANTICODON 10. RIBOSOMAL 11. TRANSFER 13. GENETIC 15. OPERON CHAPTER 11 Gene Technology 1. c; Human Genome Project 2. h; vector 3. g; plasmid 4. i; recombinant DNA 5. b; vaccine 6. e; restriction enzymes 7. d; gene therapy 8. j; gene cloning 9. f; electrophoresis 10. a; transgenic animal 11. k; genetic engineering CHAPTER 12 History of Life on Earth 1. A radioisotope is an unstable element that breaks up and gives off energy in the form of radiation; the half-life is the time it takes for half an amount of the element to change. 2. Spontaneous origin refers to the origin of life from simple chemicals; microspheres are vesicles made of short chains of amino acids and might have been the first step toward cellular organization. 3. Eubacteria are prokaryotes whose cell walls contain a chemical called peptidoglycan and have the same type of lipids in their cell membranes as eukaryotes; archaebacteria are prokaryotes that lack peptidoglycan in their cell walls and have unique lipids in their cell membrane. 4. Mutualism is a partnership in which both organisms benefit. An example is a mycorrhiza, which is the partnership between fungi and the roots of plants. The fungi provide minerals to the plant, and the plant provides nutrients to the fungi. 5. d 6. e 7. f 8. g 9. h 10. i 11. a 12. c 13. b CHAPTER 13 The Theory of Evolution 1. 2. 3. 4. 5. d a b c b Biology: Principles and Explorations Vocabulary Answer Key 69 < o B.t i £ I I •3 .S3 O I O U ».§ E « II II Biology: Principles and Explorations Concept Mapping Answer Key 49 . Date Name_ . Class . Class. CONCEPT MAPPING CONCEPT MAPPING Gene Technology History of Life on Earth Using the terms and phrases provided below, complete the concept map showing the evolution of early life forms. chloroplasts fossils prokaryotes cyanobacteria life on land 3.5 billion years ago endosymbiosis mitochondria eubacteria ozone Using the terms and phrases provided below, complete the concept map showing the uses and applications of gene technology. agriculture cloned animals electrophoresis genetic disorders genetic engineering medicines probes restriction enzymes vaccines Biology: Principles and Explorations Concept Mapping Chapter 11 Copyright © by Holt, Rinehart and Winston. All rights reserved. 11 12 Biology; Principles and Explorations Concept Mapping Chapter 12 4. 5. 6. 7. 8. 9. COMPLETION 25. genetics 26. self-pollination 27. phenotype, genotype 28. probability 29. Punnett square 30. monohybrid 31. polygenic traits 32. genetic disorders 33. Hemophilia 34. Phenylketonuria or PKU MATCHING ESSAY 35. Mendel allowed each variety of garden pea to self-pollinate for several generations, selecting plants from each generation that exhibited one form of a trait. He did this until all of the offspring of a given variety produced only one form of a particular trait. 36. Mendel calculated an approximate 3:1 ratio of contrasting traits. He derived this ratio by counting plants expressing each type of trait he was comparing. Using division, he found that the ratio of plants expressing the dominant trait to plants expressing the recessive trait was about 3:1. 37. the law of segregation and the law of independent assortment 38. In the winter, the genes of the arctic fox that code for enzymes involved in pigment production do not function because of the cold temperature. Thus, the coat of the fox is white, and the animal blends in well with its surroundings. In warmer weather, these genes function and the fox's coat darkens to a reddish brown. Fur color in Siamese cats is also influenced by temperature. The fur on the ears, nose, paws, and tail of Siamese cats is darker than the rest of their body. In plants, hydrangea flowers of the same genetic variety range in color from blue to pink, depending on the acidity of the soil. Hydrangea plants in acidic soil bloom blue flowers; those in neutral to basic soil bloom pink flowers. 39. Persons who are heterozygous for the sickle cell trait produce a defective form of hemoglobin that causes some of their red blood cells to bend into a sickle shape. If the parasitic protozoan that causes malaria infects an individual who is heterozygous for the sickle cell trait, the sickled red blood cells cause the death of the parasite. 40. The blood clotting disorder, hemophilia A, is a sex-linked trait. In hemophilia A, the mutation occurs on one of the genes on the X chromosome. The gene codes for a protein involved in blood clotting. CHAPTER 9 DNA: The Genetic Material MULTIPLE CHOICE 1. a 2. c 3. d 182 Biology: Principles and Explorations b b b d a b 10. d 11. b 12. n 13. m 14. i 15. a 16. h 17. o 18. k 19. f 20. e 21. j 22. 1 23. c 24. g COMPLETION 25. virulence 26. genetic or hereditary 27. host or bacteria 28. two 29. base-pairing 30. diffraction 31. complementary 32. replication 33. replication fork 34. S or synthesis 35. proofread ESSAY 36. The harmless R bacteria were transformed into virulent S bacteria, and the mice died. 37. His experiments showed that the activity of the material responsible for transformation was not affected by protein-destroying enzymes, but the activity was stopped when a DNA-destroying enzyme was present. Therefore, the genetic material was DNA. 38. Radioactive elements were used because they can be followed or traced. Hershey and Chase could use the radioactive elements to locate the genetic material of bacteriophages after they infected bacteria. 39. DNA polymerase proceeds along the new DNA strand only if the previous nucleotide is correctly paired to its complementary base. If a mismatched nucleotide occurs, the polymerase is able to backtrack along the new DNA strand. The mismatched nucleotide is removed, and the correct nucleotide is inserted. 40. DNA helicases are enzymes that unwind the double helix of the DNA molecule. The unwinding is accomplished by breaking the hydrogen bonds that link the complementary bases. 41. a piece of double-stranded DNA 42. A—hydrogen bonds; B—sugar-phosphate backbone; C—pyrimidine (or nitrogen base) Test Prep Pretest Answer Key a "01 o X 0. o 1-1 CHAPTER 10 How Proteins Are Made MULTIPLE CHOICE 1. 2. 3. 4. 5. 6. 7. 8. d a b a c d b c TRUE/FALSE 9. F >; 10. 11. 12. 13. 14. 15. 16. 17. 18. T F T T T F F T T \ % ., &- ft COMPLETION 19. uracil 20. RNA polymerase 21. codons 22. A 23. operator 24. represser 25. gene expression or protein synthesis 26. transcription 27. translation 28. transcription factors 29. introns 30. exons 31. point mutations 32. frameshift ESSAY £ I s o I o U 33. RNA consists of a single strand of nucleotides instead of the two strands that form the DNA double helix. RNA nucleotides have the fivecarbon sugar ribose rather than the sugar deoxyribose found in DNA nucleotides. RNA nucleotides have a nitrogen-containing base called uracil instead of the base thymine found in DNA nucleotides. 34. First mRNA binds to a ribosome. Then tRNAs carry amino acids to the ribosome according to the three-base codons on the mRNA. The amino acids are joined together to form a protein chain. 35. Messenger RNA (mRNA) is an RNA copy of a gene used as a blueprint for a protein. During translation, mRNA serves as a template for the assembly of amino acids. Transfer RNA (tRNA) molecules carry amino acids to the ribosome and act as interpreter molecules, translating mRNA sequences into amino acid sequences. Ribosomal RNA (rRNA) plays a structural role in ribosomes. 36. The lac operon consists of a cluster of genes that enables a bacterium to build the proteins needed for lactose metabolism only when lactose is present. Some of the genes determine whether or not other genes will be expressed; the other genes code for enzymes that break down lactose. 37. Eukaryotic cells contain more DNA than prokaryotic cells. Although both prokaryotes and eukaryotes use regulatory proteins, eukaryotes use many more proteins than prokaryotes do and the interactions are more complex. Instead of using operons, eukaryotic cells have genes with related functions scattered on different chromosomes. 38. Because of the existence of introns and exons, cells can shuffle exons between genes. Over time, this can result in new genes being made. For example, the twelve different hemoglobin genes in modern humans arose from one, ancestral hemoglobin gene. 39. In point mutations called substitutions, one nucleotide in a gene is replaced with a different nucleotide. An insertion is the addition of one or more nucleotides to a gene. When a deletion occurs, one or more nucleotides are deleted from a gene. 40. Mutations occur in all types of cells, including body cells and gametes. CHAPTER 11 Gene Technology MULTIPLE CHOICE 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. c a b a d c b d c c MATCHING 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 21. f i j e b k g h d a c COMPLETION 22. 23. 24. 25. gene vector complementary insulin Biology: Principles and Explorations Test Prep Pretest Answer Key 183 26. Anticoagulants 27. factor VIII 28. pathogen 29. Ti plasmid 30. glyphosate 31. DNA ligase ESSAY 32. The Human Genome Project is a research effort to identify, locate, and sequence the entire collection of genes in a human cell. 33. DNA fingerprints are used in paternity cases, forensics, and identifying genes that cause genetic disorders. 34. Because the human body contains small amounts of specific proteins that are required for proper functioning, large quantities of specific proteins needed to treat certain disorders are difficult to obtain. Genetic engineering technologies have allowed the production of large quantities of products containing the specific proteins required to treat many disorders. 35. Scientists removed the tumor-causing genes from the Ti plasmid. The vacant space in the now-harmless plasmid was then filled with specific DNA. 36. If crops are naturally resistant to insects, farmers can use fewer pesticides, which are harmful to the environment. 37. Wilmut deprived the mammary cells (the differentiated cells) of nutrients before combining them with egg cells in which the nuclei had been removed. The nutrient deprivation forced the differentiated cells to pause at the beginning of the cell cycle so that both the differentiated cells and the egg cells would be at the same stage in their cycles at the moment they were combined. CHAPTER 12 History of Life on Earth MULTIPLE CHOICE 1. 2. 3. 4. 5. 6. 7. 8. 9. d d b d d b c b a 10. 11. 12. 13. 14. c d b d c 18. F 19. T 20. T 21. F 22. T 23. T 24. T 25. T 26. F 27. T 28. T 29. T 30. T COMPLETION 31. bacteria 32. Archaebacteria 33. underwater bubbles 34. microspheres 35. animals 36. ozone 37. plants, fungi 38. mutualism 39. arthropods 40. prokaryotes or bacteria, cyanobacteria 41. genetic material, reproduction ESSAY 42. As ancient cyanobacteria carried out photosynthesis, they began to release oxygen in the oceans. After hundreds of millions of years, the water of the oceans became saturated with oxygen, and the gas began to bubble into the air. Over the billions of years that followed, more and more oxygen was added to the air. 43. It allowed "division of labor" among cells and cell specialization which lead to organism complexity. 44. Amphibians, specifically the ancestors of today's frogs, toads, and salamanders, were the first vertebrates to live on land. Their lungs enabled them to absorb oxygen from the air. A strong, flexible internal skeleton made walking possible and allowed vertebrates to grow larger than insects. 45. The primordial soup model requires more time for the formation of organic molecules than was available. Also, there was no ozone layer to protect Earth from the ultraviolet (UV) radiation that would have destroyed any of the hydrogen-containing gases needed for the chemical reactions of the model. 46. fungi and plants, arthropods, vertebrates 47. Snakes would be more likely to survive. A snake's watertight skin keeps it from becoming dehydrated. Because snake eggs are also watertight, they can survive in dry conditions. Frogs lack both watertight skin and watertight eggs. TRUE/FALSE 15. T 16. T 17. T 184 Biology: Principles and Explorations Test Prep Pretest Answer Key £