BIO310 Syllabus Plant Morphology/Embryology Fall, 2007 Next offering Fall 2009 Extension 1263 bsmith@ycp.edu Bruce B. Smith, Ph.D. LS 235 Objectives and Course Description Plant Morphology/Embryology will have as its primary objective the understanding that a flowering plant species represents the zenith in terrestrial plant evolution. Because of this, knowledge of its development from seed to seed is essential. A selected species will be studied from seed germination through development of the next generation’s seed. Vegetative development, roots, flowers, pollen, ovules, pollination and embryogenesis will be the focus in lectures. The laboratory will stress techniques of growing, harvesting, collecting and microscopic analysis of selected tissues. 4-Credits Lecture: Laboratory: Text References1: TTH: 2:00-3:15 W: 11:00-1:45, Bowman, J. Editor.1994. Arabidopsis, An Atlas of Morphology and Development. Springer-Verlag. New York. (AAMD) This atlas is intended as a reference book, both for scientists already familiar with plant anatomy and for those utilizing Arabidopsis having come from other disciplines. Johri, B.M. et. al.1992. Comparative Embryology of Angiosperms,(Vols.1 and 2). Springer-Verlag. New York (CM) This text is a review of the developmental processes leading to sexual reproduction in flowering plants, and provides illustrated source material on sexual reproduction, primitive and advanced embryological characters, and phylogenetic relationships. 1 Lecture and laboratory materials will be taken from these references. There is not a text available for this course. You will need to take notes in both lecture and lab and make arrangements, if you feel it necessary, to peruse these references. Cristi, M. and A. Tiezzi. (Editors). 1992. Sexual Plant Reproduction. SpringerVerlag. New York. (SPR) This book includes all the lectures presented during a course held in Siena, Italy in 1991. It provides an overview of the state of the art of plant sexual reproduction known at that time. Cristi, M. et. al. (Editors). 1992. Atlas of Sexual Reproduction in Flowering Plants. Springer-Verlag. New York. (ASR) This atlas contributes to our understanding of plant reproductive cell structure, and gives a striking portrayal of images throughout embryogenesis. Raven, P.H. et. al. 1999. 6th Edition. Biology of Plants. W.H. Freeman and Company Worth Publishers. New York (BOP) Raven, et.al.2008. 8th Edition. Biology. McGraw-Hill. New York, NY. A standard text for botany throughout the world and is the text for our 200 level botany. The second Raven book is the book we chose to use in BIO I and II this year. Should you need a general reference, I have copies. Copies may be available in Schmidt Library or you could purchase them. Besides the books mentioned above there would be many other references made available on the major foci of this course. Lecture material is gleaned from these and other sources. Please come by my office should you need to review any of these references. GRADING: ½ of your grade comes from the average of the lecture exams including any final exam, and the other ½ comes from your laboratory work. Attendance: You are expected to attend all scheduled lectures and laboratories. Classroom Behavior: The student handbook should be consulted. The biology department’s academic honesty and safety policies are attached to this syllabus. 8/28 8/30 9/4 9/6 9/11 9/13 9/18 9/20 9/25 9/27 10/2 10/4 10/91 10/11 10/18 10/23 10/25 10/30 11/1 11/6 11/8 11/13 11/15 11/20 11/27 11/29 12/4 12/6 12/11 TBA Lecture Schedule Vegetative Development:(BOP,C23)(AAMD, C1) Introduction, Plant Form, Ch.36 ppt. Vegetative Development (Continued), Vegetative Development Vegetative Plant Development Ch. 37. Vegetative Development (Continued) Flowers: Introduction, Inflorescence Vegetative Development Plant Reproduction, Ch. 42 Roots: Introduction; Flowers: (continued) Floral Organs, Nectaries Roots: (Continued) Root Hairs; Pollen Introduction Roots: (Continued) Root Growth; Ovules: Introduction, Ovule development Ovules: (Continued) Megasporogenesis, Zygote and Early Embryo Development Exam #1 10.1_AAMDSeedling, Seed Shoot.ppt (Con’t) 11.2_AAMDRoots.ppt (con’t) 12.3_AAMDFlowers.ppt Fall Break, Sat., 10/13-Tues., 10/16 (Con’t) 13.4_AAMDPollen.ppt Exam #2 (Con’t) 14.5_AAMD Ovules.ppt (Con’t) 15.6_AAMD POLLINATION.ppt 16.7_Embryogenesis.ppt Developmental Reports, Power Point Presentations Developmental Reports, Power Point Presentations Thanksgiving Vacation, Wednesday, 11/21-Sunday, 11/25 Developmental Reports, Power Point Presentations Developmental Reports, Power Point Presentations Developmental Reports, Power Point Presentations Developmental Reports, Power Point Presentations Developmental Reports, Power Point Presentations Final Exam, ½ from previous exams, ½ from material after exam #2 8/29 9/5 9/12 9/19 9/26 10/3 10/10 10/17 10/24 10/31 11/7 11/28 12/5 Laboratory Schedule Laboratory orientation: preparation for keeping Laboratory Journal and drawings. Arabidopsis, Introduction to plant, preparations for germination and planting. Introduction to references. Assignment of articles to obtain and discuss. Continue Aribidopsis Seeding development studies from previous week. Equipment orientation (microscopes). Introduction to clearing technique, preparation of vegetative tissues from seed germination, preparation of solutions needed, “raj”slide preparation Continue observations of developing plants, Dissection of whole preserved flowers of Brassica sp. (Discuss Articles) Collection of materials from growing Aribidopsis and Brassica sps., Procedures of fixing, dehydrating tissues. (Discuss Articles) Analysis of selected, cleared tissues. Use both dissecting and phase microscopes (Discuss Articles) Dissection of ovules from selected Brassica sp.(Discuss Articles) Procedures for capturing images using Image grabber software. Storing and retrieving files. Continue use of procedures to collect, fix, and clear tissues. (Discuss Articles) Continue work on individual project for Journal2 Continue work on individual project for Journal Thanksgiving Vacation, Wednesday, 11/21-Sunday, 11/25 Continue work on individual project for Journal Continue work on individual project and turn in Journal LABORATORY GRADE: Attendance and participation in discussion of primary articles will account for 1/3, neatness, completeness, and accuracy of laboratory journal accounts for 2/3. 2 You will need to purchase a journal notebook from the bookstore and keep impeccable notes and records of all your laboratory work which will be handed in at the end of the semester and represents a portion of your lab grade. LAB RULES: No eating, drinking, gum/tobacco chewing No open toed shoes, sandals, flip flops Wear appropriate personal protection Clean up spills and broken glass immediately Know the location of safety equipment Label all chemicals Report unsafe situations to your instructor Wash hands when you leave the lab If you’re not sure, ASK! STAY SAFE ! ACADEMIC HONESTY IN THE YORK COLLEGE DEPARTMENT OF BIOLOGICAL SCIENCES Science and the teaching of science represent a search for truth and they rest on ethical behavior and intellectual honesty. As such, both the Department of Biological Sciences and York College of Pennsylvania unequivocally condemn academic dishonesty. Academic dishonesty is defined in the York College Student Handbook as “all situations where a student makes use of the work of others and claims such work as his/her own.” Because the Department of Biological Sciences maintains high expectations for all students and is committed to stringent standards of academic honesty, we contend that all published information, in any form, must not be used unless rigorously paraphrased and properly cited. Moreover, all tests, projects, assignments, and lab reports require a solo effort unless specifically noted otherwise by the instructor. This means that the sharing of text, images, tables, figures, or data analyses with classmates is a breach of academic honesty. Furthermore, providing such information to others will be considered as dishonest as accepting or taking the information. Work done in lab may involve partners, but the formal partnerships end when the laboratory period ends. At the end of a lab, each partner should leave with his or her group’s protocols, hypotheses, data, and any information about procedural problems. Once the in-lab work is completed, the work shifts from a group effort to a solo effort. This does not mean that students shouldn’t discuss lab concepts, problems, and general strategies and broad interpretations. Talking about science is healthy and is encouraged. And, it is understood that lab groups may obtain similar or identical quantitative data for a given project. In the end, however, data analyses and report writing as well as the overall presentation and interpretation of these data are to be done independently by the individual and not by the group. If work submitted by two or more students appears unexplainably and unreasonably similar, academic dishonesty will be assumed. In this event, the instructor will provide written notification to the student, the Department Chair, and the Dean of Academic Affairs of the charge and the sanction. Documentation related to instances of academic dishonesty will be kept on file in the student’s permanent record. If the academic dishonesty is the student’s first offense, the instructor will have the discretion to decide on a suitable sanction up to a grade of 0 for the course. If the Dean of Academic Affairs determines that the academic dishonesty is the student’s second offense. The Student Welfare Committee will automatically conduct a hearing to review the charge and decide on an appropriate sanction, which will involve academic suspension or dismissal from the College. Students are not permitted to withdraw from a course in which they have been accused of academic dishonesty. If questions about academic honesty arise, see the course instructor before completing and submitting your work. In addition, specific information about the York College of Pennsylvania Academic Dishonesty Policy can be found in the most recent edition of the Student Handbook. Sign below: I, _________________________, have read this statement and the syllabus for BIO152 and I understand and accept departmental and college expectations of academic honesty and ethical conduct. Last updated August 2005