National 4 -Energy from the sun Green plants are known as producers as they can make their own food using raw materials from the environment and energy from sunlight. The process by which green plants do this is known as photosynthesis and the food they make is carbohydrate. Animals are known as consumers as they can’t make their own food and eat green plants. Energy from the sun sun sun Raw materials from the environment Green plants make carbohydrate by photosynthesis Animals eat green plants CO2 National 4 and 5- Trapping light To carry out photosynthesis plants must be able to capture light from the sun. Plants do this using a special pigment called chlorophyll. Chlorophyll is found within structures called chloroplasts that are in the cytoplasm of certain plant cells. National 4 chloroplasts containing chlorophyll cytoplasm 1 National 4 and 5- Photosynthesis When plants carry out photosynthesis they use water from the soil and carbon dioxide from the air as raw materials and sunlight trapped by chlorophyll to provide the necessary energy. Carbohydrate is produced in the form of glucose and oxygen is produced as a by-product. This can be shown as a word equation: carbon dioxide + water raw materials light energy glucose + oxygen chlorophyll conditions required products National 4 and 5- Affects of light on photosynthesis When plants photosynthesise they make carbohydrate in the form of glucose which is then stored as starch. Therefore it can be assumed that if a plant has starch in its leaves then it has been photosynthesising, while if it does not have starch in its leaves then it has not been photosynthesising. The following experiment shows the effects of light on photosynthesis: 1. Place one plant in bright light and another in a cupboard for a few days water 2. Use a cork borer to remove a disc from each leaf and place them in labelled tubes of water as shown: 3. Place the tubes in a beaker of water and heat the water until it boils. 4. Turn the Bunsen off leaf disc from plant in bright light leaf disc from plant in cupboard 5. Pour the water out (use a wooden splint to hold the leaf discs in place), add enough ethanol to cover the leaf discs and place the tubes back into the beaker of water. 6. Once the green colour has been removed from the leaf discs, hold the disc in place with a wooden splint and pour the ethanol into the waste ethanol bottle. National 4 2 7.Use iodine to test the leaf discs for starch. Results Conclusion The leaf from the plant that had been in the light contained starch but the leaf from the plant that had been in the dark did not. This shows that plants must have light in order to carry out photosynthesis and produce glucose which is stored as starch. National 4 and 5- Affects of carbon dioxide on photosynthesis In order to make glucose, and subsequently starch, by photosynthesis plants must have carbon dioxide as a raw material. How much starch a plant can produce by photosynthesis is directly affected by how much carbon dioxide the plant has available to it. The following experiment is set up to show the effects of carbon dioxide on photosynthesis: Sealed bell jar Plants are well watered and kept in bright light for 1 week Potassium hydroxide which removes CO2 from the air Sodium bicarbonate which adds CO2 to the air After one week a leaf from each plant was tested for starch using the same method as in the affects on light on photosynthesis experiment. National 4 3 Results and conclusion The plant that had a high quantity of carbon dioxide available to it had lots of starch in its leaves while the plant that had a low quantity of carbon dioxide available to it had no starch in its leaves. This shows that the process of photosynthesis is dependant on carbon dioxide being available and if it is not then photosynthesis cannot be carried out and starch is not produced. Limiting factors and photosynthesis Photosynthesis will be slowed down under the following circumstances 1. If light or carbon dioxide is in short supply 2. If the temperature is too low or high Between points A and B the the rate of photosynthesis is increasing and is not going at its optimum rate. This means that light intensity must be limiting the rate of photosynthesis, so between points A and B light intensity is a limiting factor. After point B the rate of photosynthesis does not increase. This means that photosynthesis is being carried out as fast as the plant can, so after point B light intensity is not a limiting factor. National 4 Rate of photosynthesis Whichever factor is responsible for slowing down photosynthesis (carbon dioxide, light or temperature) is known as a limiting factor. The rate of photosynthesis will increase until it reaches a maximum then it will level off. Example of a limiting factor B A Light intensity 4 Overcoming limiting factors There are a number of ways to prevent carbon dioxide, light intensity or temperature becoming a limiting factor: Potential limiting factor Light Carbon dioxide Temperature Way of overcoming Supplementary lighting (lighting other than normal daylight) Supplementary lighting Plant s When paraffin or propane are burned they give off carbon dioxide. By using heaters that burn paraffin or propane carbon dioxide can be supplied to the plants Parafin/ propane heater Heating Advantages – the grower can produce crops earlier in the year than normal - higher yields are possible Disadvantages – these methods require electricity and fuel and are therefore expensive Level 4 E&O Nat 4 National 4 Photosynthesis- limiting factors If any of the requirements (light, water carbon dioxide or suitable temperature are low or missing the photosynthesis rate is limited. By overcoming these limitations, faster growth rates can be achieved 5 Energy Living cells cannot survive without energy as it is required for numerous functions carried out by the cell e.g. Cell Division Uptake of chemicals Cell Growth Energy Building up large molecules Muscular contraction Respiration While energy can be released from many different foods, the main food molecule used as a energy source in cells is glucose. However if cells released energy from glucose in one large quantity as it is above, it would result in damage to the cell. Cells avoid this by using a process known as RESPIRATION to release energy from glucose in a large number of small steps. Each of these steps is controlled by a different enzyme. As this is controlled by enzymes the rate of respiration will be effect by temperature (refer back to enzymes). Respiration takes the chemical energy from glucose and transfers it into other molecules in the cell. Any energy which is not transferred into these cell molecules is released as heat energy. If oxygen is available to cells then they will use aerobic respiration to break down glucose and release energy. Aerobic respiration is the process where cells use oxygen to break down food and release energy. National 4 6 In animal cells lactic acid is porduced during Aerobic respiration. In plant and yeast cells ethanol and carbon dioxide are produce during Aerobic respiration. Investigation into anaerobic respiration in yeast Background In the absence of oxygen yeast will respire anaerobically and produce ethanol and carbon dioxide. Method Thermometer 1. Boil a solution of glucose and allow it to cool To water pump Insulation 2. Set up the apparatus as shown: Layer of oil Lime water 3. The oil is added to prevent oxygen being added. 4. The insulation is added to prevent heat escaping. 5. Let experiment run for 24 hours Boiled and cooled glucose solution and yeast Thermometer Results Smell of flask Appearance of lime water Temperature of glucose solution (oC) At start Sugar clear 21 oC After 24 hours alcohol cloudy 26 oC Conclusion As there was no oxygen in the glucose solution we would expect the yeast to use anearobic respiration and produce ethanol and carbon dioxide. The experiment National 4 7 proved this as after 24 hours the the flask smelt of alcohol and the lime water had turned cloudy. National 4 - Comparison of aerobic and anaerobic respiration Aerobic respiration (oxgen available) Respriatory substrate Glucose Anaerobic respiration (no oxygen) In animals In plants and yeast glucose glucose Products Carbon and water dioxide Lactic acid Carbon and ethanol dioxide Energy Large number Little Little Level 4 E&O I can contribute to the design of an investigation to show the effects of different factors on the rate of aerobic respiration and explain my findings. Nat 4 National 4 Factor affecting respiration Respiration is used to release energy for use in cells. Oxygen may or may not be used in both yeast and animal cells. When oxygen is available, yeast plant and animal cells use oxygen to produce carbon dioxide and water. Without oxygen yeast and plant cells use glucose to produce alcohol and carbon dioxide. Without oxygen animal cells use glucose to produce lactic acid. More energy is released per molecule of glucose when oxygen in present. The process in enzyme controlled in all cases so is affected by temperature. 8 National 4 9