Doing a brilliant A2 Biology Coursework in Ecology. The A2 coursework builds on what you did at AS, but with some added twists: all your marks must come from one investigation (no pick & choose!), you need to state a proper hypothesis, you must use a correct statistical test for the higher marks, you must bring together relevant concepts from different topics for a new skill ‘E’. Follow these guidelines step-by step, & keep thinking … What you must do Explanation / example criterion Research the idea you have been given. Find out: General info about your species, Info about the habitat, What abiotic factors affect your species, What biotic interactions may affect it. Sketch the graph you would like at the end, with labelled axes (units!) for your input & output variables. Sketch a results table for the right data, and Choose the right statistical test NOW! Jot down all other variables you will need to take into account, and Jot down key points of your method Your H1 should be simple, clear and testable: it should state how the input variable will affect the output variable. Eg: ‘dogwhelks will require more force to dislodge them on shores more exposed to wave action’ the H0 (neutral, not opposite to H1) will be used as the basis for your stats test. Take care that all the information is relevant to your investigation, and you say how it is relevant you include concepts from other areas; e.g. temperature needs controlling because it affects enzymes & therefore … The most likely ‘other areas’ are: osmosis (re salinity), enzymes (re temperature / pH), respiration (via enzymes), photosynthesis (re light intensity). you present you ideas clearly and logically, using technical terms where appropriate E Think backwards to clarify your ideas! Don’t skimp this stage or you’ll regret it later (e.g. when 10 hours worth of data can’t be put into a statistical test!) Write your hypothesis (H1) and a null hypothesis (H0). Write an introduction, giving the background information you are using in planning your investigation. 106747358.doc page 1 All! A E 03/03/2016 Write your method Collect your data… … but don’t stop thinking now! Briefly discuss the precautions taken while collecting data Prepare your data Plot a graph before doing the stats Describe the pattern / trend 106747358.doc Use numbered steps, Use the future (impersonal) tense, e.g.: ‘50 dogwhelks will be chosen …’ Work individually, under supervision Give clear details of method, apparatus & precautions (as if someone else had to follow your plan) Explain your choice of technique Explain any necessary preliminary checks (e.g. for quadrat size, No of stations, etc) Say how you will minimise the effect of each of the ‘other factors’ (&/or measure them for later discussion) Say what data you will collect: how many samples, what units … Say what stats test you will use and why you chose it (re type of data) Any other analysis – e.g. graphs? Use a proper table, with headers & units Always keep & work from your raw data – copying it usually introduces errors Look at the data as you collect it: Does it look consistent? o If not, can you improve anything? Do you need to o sample more areas, or o fewer areas more thoroughly? o take some extra repeats? Is another factor affecting your results? o can you improve your method? o can you measure it? Don’t be afraid to stop collecting data if it looks highly significant already and you have a good sample size! I.e.: say what you were able to do (and could not do) to ensure reliable data. Do the totals, averages, percentages Give an example of your working Use units, signs (+/-) and sensible accuracy e.g. don’t give the average force to remove a whelk as 15.6723639 N! A simple graph (bar, line, or scatter) will give you a ‘feel’ for your results and what they show You will be less likely to believe a daft stats result if you know what the graph shows! Most studies show some trend: even if it is ‘non- page 2 B2,4,6 B8 C2, 4 C4 03/03/2016 shown by your graph Now that evil statistical test! But you chose the right test at the start, and you’ve got your Numerical Techniques book, so it will be a doddle! Write a conclusion. Just the evaluation left… State all the main limitations of your method. Assess the likely effects of each limitation on your data. Assess the overall validity of the data You’ve finished! 106747358.doc significant’ it is still a trend! Do consider the details, as well as the basic ‘up’ or ‘down’ – this is A2, after all! Either do it long-hand, and show the steps, or use a stats pack and state: exactly what test you did, on what package, the formula for the test what data you entered (totals, %, etc) how many degrees of freedom, the value for the statistic (e.g. t=3.145) the p-value (e.g. p<0.001) what the p-value means for your H0 & H1 (‘rejected’ or ‘supported’ not ‘proved’) This must be clearly based on your results, graph, and statistics. Summarise what you have found out. In detail, say how this relates to your hypothesis, and Explain your results using the biological ideas in your introduction (& any others?) … but it’s not like GCSE. This means the main unavoidable limitations inherent in doing it this way (e.g. ‘the shores differed in aspect as well as exposure to wave action’; but no marks for ‘human error’, ‘out of time’ ‘a sheep ate my data’, etc) How is the accuracy, or overall reliability likely to have been affected? E.g.: ‘whelks on the S-facing shore may be warmed by the sun, respire faster & thus hold on tighter, but the trend still held for 10 that were in complete shade on this shore…’ C6 C6 D2 D4 D6 For each limitation, explain whether it would undermine the overall conclusion. Don’t be too harsh on yourself – most studies should still be valid, and limitations are unlikely to overturn highly significant results. Round it off with a sentence giving your verdict on the validity of your study. The End. page 3 03/03/2016