MAKERERE UNIVERSITY FACULTY OF SCIENCE MASTER OF SCENCE DEGREE PROGRAMS JUNE 2009 CURRICULUM FOR THE MASTER OF SCIENCE IN BIOCHEMISTRY (MSc Biochemistry) COURSE-WORK AND DISSERTATION (PLAN A) DAY PROGRAMME Course Name: Course Code: Course Level: Course Credit: MICROBIAL BIOCHEMISTRY AND PHYSIOLOGY MBC7101 1 4 CU Brief Course Description This course examines: a) the principles underlying microbial systematics, b) microbial growth and metabolism, c) information flow, and d) signal transduction and homeostasis. Course Objectives At the end of this course learners should be able to: Isolate, characterize, and classify microorganisms Discuss the various approaches to microbial systematic Use mathematical models to describe the kinetics of microbial growth Discuss microbial signal transduction and homeostasis Describe a microbial genome Describe mutation, mutagenesis, mutants and mutation analysis Discuss the molecular basis of mutations Compare the prokaryotic and eukaryotic genomes Course outline Microbial Systematics (10 hours) Approaches to microbial taxonomy: a) Numerical taxonomy (based on morphological, physiological and biochemical characteristics), b) phylogenetic or molecular taxonomy (based on rRNA cataloguing, mol % G+C content, DNA: DNA hybridization), c) classification of bacteria (eubacteria and archeabacteria), d) taxonomic criteria of fungal classification, and e) viruses classification Microbial growth, physiology and metabolism (10 hours) a) Growth kinetics, mathematical models for batch (closed) and continuous (open) culture system; b) Microbial physiology and metabolism: overview of primary metabolism, secondary metabolism and microbial signal transduction and homeostasis (quorum sensing, chemotaxis and efflux systems) Microbial genetics (15 hours) a) An overview of the organization of the prokaryotic and eukaryotic genomes, Typical examples of bacteria genome (haploid genome) and the eukaryotic yeast genome(diploid) b) The gene: organization (the operon model), and gene expression or protein synthesis, control/regulation of gene expressions (transcription and translation control mechanisms, post-translational control , global regulators and involvement of RNA in the regulation of gene expression); c) Bacterial recombination: molecular basis of recombination, conjugation, transduction, complementation and transformation; d) Extra-chrosomosomal elements: plasmids/cosmids, transposons and phages; e) Mutation: Mutagens (chemical mutagens, ionizing radiations), mutagenesis (chemical mutagenesis, radiation mutagenesis, PCR-based mutagenesis methods) and mutants, molecular basis of mutations, isolation of mutants, determination of mutation rates. Tutorial (20 hours) Practical (30 hours) Mode of delivery This course will be taught by using lectures, practical and coursework assignments. Assessment Assignments, reports, tests, practical reports and end of module examination. Their relative contribution to the final grade is shown below: Requirement Contribution Progressive (Practicals and assignments) 20 % Test 20 % Final examination 60 % Total 100 % Reading List LENGELER, J. W., DREWS, G AND SCHLEGEL, H. G (1999). The Biology of the Prokaryotes SINGLETON, P (1997). Bacteria in Biology, Biotechnology and Medicine. Course Name: Course code: Course Level: Course Credit: GENETIC ENGINEERING AND BIOTECHNOLOGY MBC7102 1 3 CU Brief Course Description This course begins by introducing students to the concept of genetic engineering and biotechnology. It then examines the molecular cloning methods, the various cloning vectors and their hosts, and how to find the right vector for molecular cloning. Aspects of DNA amplification and analysis techniques, cloning and expression of mammalian and plant genes in bacteria and practical applications of genetic engineering and biotechnology are covered under this course. The course ends with the analysis of the legal, ethical, and biosafety aspects of genetic engineering. Course Objectives At the end of this course learners should be able to: Explain the concept of genetic engineering and biotechnology Explain the principles underlying molecular cloning Describe the various cloning vectors and their hosts Describe the principles underlying DNA amplification and analysis Explain the steps involved in cloning and expression of mammalian and plant genes in bacteria Describe the various practical applications of genetic engineering and biotechnology in agriculture, industry, medicine and environmental protection Analyze the legal, ethical, and bio-safety implications of genetic engineering. Course outline Concept of genetic engineering and biotechnology hours) (2 Definition of genetic engineering and biotechnology, and how have they emerged from other sciences. A historical perspective of biotechnology, and the key events in the evolution of biotechnology. Cloning, expression and analysis of genes and their products hours) (7 Concept of molecular cloning, plasmids as cloning vectors, Bacteriophage Lambda as a cloning vector, hosts for cloning vectors, finding the right vector, expression vectors. Synthetic DNA, Amplifying DNA: The polymerase chain reaction (PCR). Cloning and expression of mammalian and plant genes in bacteria, In vitro and site-directed mutagenesis. Practical applications of genetic engineering and biotechnology (7 hours) Production of Transgenic plants and animals, Production of enzymes and medicines (industrial applications), Production of Mammalian Products and Vaccines by Genetically Engineered Organisms (GMOs), Use of GMOs in pollution control (bioremediation). Legal, ethical and bio-safety aspects of genetic engineering (4 hours) Institutional, National and international legal framework for biotechnology, ethical concerns of genetic engineering (culture, religion and trade), safety and risks of genetically engineered products to human health and environment. Tutorials (20 hours) Practical hours) (30 Mode of course delivery This course will be conducted in three main ways i.e formal lectures, reading assignments/coursework, and participatory discussions/presentations. Assessment End of module examination, tests, assignments reports, and presentations. Their relative contribution to the final grade is shown below: Requirement Contribution Progressive (Practicals and assignments) 20 % Tests 20 % Final examination 60 % Total 100 % Reading List The recommended reading will include but not limited to the following literature. Michael T. Madigan, John M. Martinko and ack Parker (2000). Brock. Biology of microorganisms (9th and 10th Editions), Prentice Hall International, Inc. Joseph Kyambadde (2005). Optimizing processes for biological nitrogen removal in Nakivubo wetland, Uganda. PhD Thesis, Royal Institute of Technology, Stockholm, Sweden, ISBN 19-7283-962-7 Essential Biosafety (CD-ROM, 2nd edition): The latest scientific and regulatory information for genetically modified and other novel crops and foods. AGBIOS, Canada http://www.agbios.com/main.php Course Name: ADVANCED ENZYMOLOGY Course code: MBC7103 Course Level: 1 Course Credit: 3 CU Brief Course Description This course begins with the Extraction and purification of enzymes, Enzyme activity, Factors affecting enzyme activity, enzyme inhibition. Enzyme kinetics: Multisubstrate enzyme mechanisms (Chance mechanisms, Ping-pong mechanisms, Random sequential and ordered Bi-Bi mechanisms). Mechanisms in families of enzymes (Serine proteases, Dehydrogenases, Carboxypeptides, Lysozyme), Multisite and Allosteric enzymes (Noncooperative sites, Cooperative binding in allosteric enzymes, the Hill equation for Allosteric enzymes, Sigmoidicity of velocity curves) Enzyme regulation and stability, Fine control of enzyme activity (Steady state fluxes, and flux regulation by feedback, Saturated or substrate independent reactions, Kacser and Burns theory, switch mechanisms, role of near equilibrium reactions in maintaining metabolite concentrations).Finally the course ends up with measurement of enzyme rate constants (Rapid mixing and sampling technique and Relaxation methods). Course Objectives At the end of this course learners should be able to: Explain the methods of extraction and purification of enzymes. Explain the standardized reporting of enzyme activities (International units, katals, turnover number) Describe factors that affect enzyme activities and types of enzyme inhibitions Describe enzyme kinetics of multisubstrate enzyme mechanisms Explain enzyme reaction mechanisms used in selected families of enzymes. Explain the binding of substrates to multisite and allosteric enzymes Explain enzyme fine regulation and stability. Describe the theories of Kacsaer and Burns, Switch mechanisms and near equilibrium reactions. Describe methods used in measuring enzyme rate constants. Course outline Extraction and purification methods for enzymes (4 hours) Description of available extraction and purification methods, and show how specific activities of enzymes. The objective of each step is to retain as much enzyme as possible while getting rid of unwanted contaminants. The efficiency of each step is given by yield and increase in specific activity. Factors that affect rates of enzyme activities and types of enzyme inhibitions (8 hours) Detailed explanation of factors that affect rates of enzyme activities, fine control of enzyme activities such as steady state fluxes, flux regulation by feedback, enzyme kinetics involving multisubstrate enzyme mechanisms involving random sequential mechanism, ordered BiBi reaction mechanisms, Ping Pong kinetic mechanism and Theorell-Chance mechanism Enzyme reaction mechanisms used in selected families of enzymes (8 hours) Detailed description of enzyme reaction mechanisms used by Serine proteases, Dehydrogenases, Carboxy peptidases and Lysozymes. Binding of substrates to multisite and allosteric enzymes involving cooperative and non-cooperative sites, and Hill equation for allosteric enzymes and sigmoidicity of velocity curves. The theories of Kacsaer and Burns, Switch mechanisms and Near equilibrium reactions hours) (5 The theories of Kacser and Burns, switch mechanisms and near equilibrium reactions will be explained. The principles learnt in the mechanisms are explained in rapid mixing and relaxation methods used in measuring enzyme rate constants. Tutorials (10 hours) Practical (30 hours) Mode of course delivery This course will be conducted in four main ways namely: Structured lectures, Coursework, practical, and Tutorials. Assessment The following instruments (Test, presentations and examination) will be used to assess the students. Their relative contribution to the final grade is shown below: Requirement Contribution Progressive (Practicals and assignments) 20 % Tests 20 % Final examination 60 % Total 100 % Reading List The reading list includes but not limited to the following textbooks. i) Lehninger ,A.L, Nelson, D.L., and Cox, M.M. (1993) Principles of Biochemistry 2nd Edition. Worth Publishers, New York. ii) Stryer, L (2005) Biochemistry. 5th Edition. W.H. Freeman and Co. New York Course Name: Course Code: Course Level: Course Credit: ADVANCED METABOLISM MBC 7104 1 3CU Brief course description This course will explore the principles that underlie the integration of metabolism in mammals. It will also examine metabolism under stress and disease, and compare metabolism in different organisms and their applications for drug design and therapeutics. The concepts and key mediators of signal transduction will also be examined. Course Objectives At the end of this course, learners should be able to: Critically discuss metabolism under stress and disease conditions, Compare the metabolism in different organisms (ruminants, non-ruminants, micro-organisms), Discuss the importance of various metabolic pathways in drug, vaccine and therapeutics Development, Describe how signal transduction cascades mediate the sensing and processing of stimuli and how molecular circuits integrate diverse external signals to generate responses. Course outline Metabolism under stress and disease (16hours) An overview of metabolism of major nutrients; implications of reaction stochiometry; the phenomenon of reverse electron transfer; metabolism under prolonged starvation, strenuous exercise, extreme temperatures, pregnancy and high altitude with limited oxygen. Metabolic adaptation, metabolism in plants under stress (salinity, drought, pathogenesis, heavy metals); metabolic control mechanism; changes in metabolism due to disease states and implication of the remedies; interconnection of pathways in metabolism, major organ systems and their specialized metabolic roles will be discussed. Comparison of metabolism in different organism (6 hours) Metabolism in protozoa, worms and bacteria will be compared; ruminants and nonruminants and application of different metabolic pathways in drug, vaccine designs and therapeutics will be discussed. Signal transduction and homeostasis (8 hours) Signal transducing receptor classes, receptor tyrosine kinases, Non- receptor tyrosine kinases, phospholipids, phospholipases, G-protein coupled receptors, G-protein regulators, intracellular hormones, Ca2+ as a cytosolic messenger will be considered. Mechanisms of signal transduction and defects in signalling; cytokine signallingcytokine receptor/receptor families (EGF-cytokine receptor, PDGF-receptor,TGFβreceptors); mechanisms by which intracellular signalling is initiated will be discussed Practical hours) (30 Teaching and assessment pattern Mode of Delivery This course will be taught using lectures, reading assignments and practical classes. Assessment method This will include assignments, tests, laboratory practical reports and end of module examination. Their relative contribution to the final grade is as shown below: Requirement Contribution Progressive (Practicals and assignments) 20% Tests 20% Final Examination 60% Total 100% Reading list J.G Salway (1999). Metabolism at a glance, 2nd edition. Frayn K.N and Keith N (1996) Metabolic regulation: A human perspective Marshell William and Stephen K Bangert (1995) Clinical Biochemistry: metabolic and clinical aspects Charles Scrivener (1995) Metabolic basis of inherited disease Volume 1-3, 7th Edition Stryer (1992). Biochemistry. 5th edition, W.H freeman and Company, New York. Lehninger, Nelson and Cox (1993). Principles of biochemistry. 2nd edition, Worth Publishers, New York. Voet D., Voet J., Pratt C.(2006). Fundamentals of Biochemistry, life at molecular level. 2nd edition published by John Wiley and Sons,Inc. Murray, Granner, Mayes, Rodwell (2003). Harpers Illustarated Biochemistry, 26th edition,Mcgraw-Hill Companies U.S.A. Course Name: Course Code: Course Level: Course Credit: STATISTICAL DATA ANALYSIS MBC7201 1 3 CU Brief Course Description This course introduces students to statistical methods often used in scientific data analysis. It begins by giving a statistical view to students and then introduces them to the basics of descriptive statistics. The probability theory and concept, probability distributions and statistical inference are also covered. Students are then introduced to hypothesis testing, comparison of two mean values, basics of experimental design and one way Anova. Significance of the F test, experiments with a block structure, factorial experiments, random and hierarchical models, split-plot experiments and checking the assumptions in Anova are also covered in this module. Course Objectives At the end of this course learners should be able to: Explain the principles underlying the various statistical methods used for data analysis Analyze scientific data using various statistical approaches Apply statistical models during their experimental design process Report their data collected in a scientific way backed up by statistical analysis Course outline Statistical view and basics of descriptive statistics (4 hours) Statistics and biological sciences, populations and samples and statistical inferences, making measurements, summarizing numerical data, graphical summaries of numeric data, and summarizing qualitative data. Probability and probability distribution (4 hours) The probability concept, probability rules, discrete random variables, continuous random variables, rules for expectations and variance and the distribution of the mean. Statistical inference and hypothesis testing (5 hours) Point estimation, properties of estimators, interval estimation, testing the hypothesis, the p-value of a test, single-sided alternative hypothesis, testing Ho: µ = µo when σ is unknown. Comparing two mean values and basics of experimental design (5 hours) Inference on µ1 – µ2: matched data, inference on µ1 – µ2: principles, inference on µ1 – µ2: small samples but equal variances, inference on µ1 – µ2: small samples but unequal variances, inference on µ1 – µ2: large samples, Minitab example: comparing two groups; key concepts in experimental design, examples of experimental designs One-way ANOVA and significance of the F test (5 hours) Introductory example, model restrictions, subdivisions of Sums of Squares, ANOVA table, analysis by computer; comparisons between the treatments, problems when you make many tests, recommendations. Block structure and factorial experiments (3 hours) Randomized block design, Latin Square experiments, two fixed factors in blocks (factorial experiments), experiments with more than two fixed factors, unbalanced, and experiments. Introductory example, model restrictions, subdivisions of Sums of Squares, ANOVA table, analysis by computer; comparisons between the treatments, problems when you make many tests, recommendations. Random and hierarchical models (3 hours) Models with random factors (one-way Anova, two-way Anova), hierarchical models (Crossed and nested factors, some examples). Split-plot experiments (3 hours) Introductory example, Model and Anova table, Analysis by computer. Checking the assumptions in Anova (3 hours) Analysis of the residues, normality, homoscedasticity, what happens if the data is not independent, validity of the model (outliers), and residual plots in MINITAB Tutorials (20hours) Mode of course delivery This course will be conducted in three main ways i.e. formal lectures, reading assignments/coursework, and participatory discussions/presentations. Assessment End of module examination, tests, assignments reports, and presentations. Their relative contribution to the final grade is shown below: Requirement Contribution Assignments/presentations % Tests % 10 Final examination % Total 100 % 70 20 Reading List The recommended reading will include but not limited to the following literature. Ulf Olsson and Ulla Engstrand (2002). Statistics for Biologists. Swedish University of Agricultural Sciences, Department of Biometry and Informatics Any other statistics text books Course Name: Course code: Course Level: Course Credit: BIOINFORMATICS MBC 7202 1 2 CU Brief Course Description This course starts with introduction to Bioinformatic, what it can and why it is now very important. This will be followed by introduction to DNA databases (NCBI, EMBL, DDBJ), Protein databases (GenPept, TrEMBL, Swiss Prot, PIR, ExPasy, SMART, etc). Search tools for data retrieval (Entrenz, DBGET, PubMed), Sequence alignment soft ware programmes (BLAST, FASTA, etc), Sequence alignment types. Main sequence types (mRNA, cDNA, genomic DNA ESTs, GSS) and end with Phylogenic analysis and Structural modelling. Course Objectives At the end of this course learners should be able to: Define bioinformatics and explain what it can do Describe the various DNA and Protein databases. Explain the principles search tools for data retrieval Explain soft ware programmes available for sequence alignments Explain the sequence alignment types Describe the main sequence types. Explain Phylogenic analysis and structural modelling. Course outline Concept of bioinformatics hours) (6 Definition of bioinformatics, what it can do, and how it has influenced research. Different Nucleic acid databases such as the National Centre for Biotechnology Information (NCBI), European Molecular Biology Laboratory (EMBL), DNA Data Bank of Japan (DDJ), and GeneBank store DNA sequences. Search tools for retrieval of data namely: Sequence Retrieval System (SRS), Entrez and DBET will be explained and demonstrated using Internet. Soft ware programmes available for sequence alignments (6 hours) The practical use of software programmes (BLAST, FASTA, etc) for sequence alignments Will be demonstrated using a computer connected to the internet, sequence alignment types such as Local sequence alignment, Global sequence alignment and multiple sequence alignment will be explained. Main sequence types in the GenBanks hours) (3 Main sequence types in the GenBanks are mRNA, cDNA, genomic DNA ESTs and GSS will be explained. The course will end with Phylogenic analysis and Structural modeling. Tutorials hours) (10 Practical hours) (20 Mode of course delivery This course will be conducted in three main ways involving formal lectures, practical Assignments using computers/Coursework, and Presentations. Assessment Assessment will be by examination, tests, assignments reports, and presentations. Their relative contribution to the final grade is shown below: Requirement Contribution Assignments and presentations 20 % Tests 20 % Final examination 60 % Total 100 % Reading List 1. Bioinformatics, A Practical Guide to the Analysis of Genes and Proteins. Second edition, A john Wiley & sons, InC., Publications Edited by Andreas D. Baxevanis and B.F.Francis Ouellette 2. NCBI databases and tools http://www.ncbi.nlm.nih.gov 3. BLAST http://www.ncbi.nlm.nih.gov/BLAST/ 4.ENTREZ http://www.ncbi.nlm.nih.gov/Entrez/ Course Name: MEDICAL BIOCHEMISTRY Course Code: MBC 7203 Course Level: 2 Course Credit: 4 CU Brief Course Description This course requires prior knowledge of the basic biological sciences at BSc or equivalent level. The course will teach an understanding of the pathophysiology of human diseases and equip students with the practical skills to pursue research and development into novel concepts of diagnosis, natural history of disease and innovative approaches to management and care for the sick. Course Objectives At the end of this course students should understand: Fluid and electrolyte homeostasis. Endocrine regulation of energy mobilisation, metabolism and reproduction. Haemopoiesis and haematological disorders. The physiological response to infection and trauma. The diseases of the vital organs (liver, kidney and heart). Genetic markers of disease and the principles of disease screening. Inborn errors of metabolism and management of perinatal conditions. Clinical Biochemistry at the extremes of life. Course outline Homeostasis (5 hours) The balance of the internal environment is an interplay of fluid and electrolyte homeostasis. The course will detail the physiological processes, which regulate these and their role in disease and management of clinical emergencies. Haematology and Inflammation (8 hours) The course will cover the regulation of haemopoiesis and the haematological disorders. It will also deal with the physiological response to infection and trauma with emphasis on the balance between inflammatory acute-phase response and the resolution of infection. Diseases of the vital organs (8 hours) A number of specific areas of medical importance will be covered under the theme of diseases of the vital organs. This will give the students an understanding of the coordinated roles of the kidney, lung and heart in acid-base balance and cardiorespiratory function. The course will highlight the importance of electrolyte balance for the maintenance of normal electrical activity of the heart and clinical emergencies that arise due to electrolyte imbalance. Laboratory case management will be illustrated for investigating injury or functional deficit of vital organs and monitoring in critical care. Genetic diseases (4 hours) The students will get an understanding of the genetic basis of disease, their diagnosis and management. Thy will grasp the use of disease markers and the principles of prenatal diagnosis and screening for disease. This will include common inborn errors of metabolism and paediatric clinical chemistry. Clinical Chemistry at the extremes of life (5 hours) The course will induct students on population, age and gender factors on reference values. They will then cover life support and the management of pre-term babies with regard to common clinical indications such as respiratory distress, hyperbilirubinemia and complications arising from pregnancy diabetes. This will be compared with care of the elderly and diseases of old age. Tutorials (30 hours) Practicals (30 hours) Mode of delivery This course will be taught by using lectures, practicals and coursework assignments and tutorial. Assessment Assessment will be done through coursework assignments and practical reports, which will constitute a progressive score, and end of module examinations. Their contribution to the final grade is shown below: Components Contribution Progressive (Practicals and assignments) % Test % Final examination % Total % 20 20 60 100 Reading list: 1. Introduction to Clinical Chemistry. Derek A Woodrow. Butterworths 2. Lecture notes on Clinical Biochemistry. AF Smith, GJ Beckett, SW Walker, PMW Rae. Sixth Edition; Blackwell Publishing. 3. Clinical Chemistry in Diagnosis and Treatment. Joan F Zilva, Peter R Pannal, Philip D Mayne. Fifth Edition. Edward Arnold. Textbook of Clinical Chemistry. Edited by Norbert Tietz. WB Saunders Course Name: ENVIRONMENTAL AND INDUSTRIAL BIOTECHNOLOGY Course Code: MBC7204 Course Level: 2 Course Credit: 4 CU Brief Course Description This course examines the important aspect of microbiology i.e. how are they amenable to man? Or how can man make microbes amenable? Advances in this aspect of microbiology are explored in such fields as environment, industry, and biotechnology. The inter-relationships of micro-organisms in nature are also explored with regards to nutrient cycling. Course Objectives At the end of this course learners should be able to: Discuss the microbial associations in different environments/ habitats Explain the principles underlying nutrient cycling Describe the application of microorganisms in nutrient cycling Relate the metabolic activities of microorganisms to their potential use in solving the pollution problems (bioremediation) Design a bioremediation strategy Assess microbial potential for use in biotechnology innovations Describe the applications of microorganisms in production of industrially valuable products Course outlines Microbial Ecology (6 hours) Microbial habitat/niches (terrestrial, marine and air), microbial associations/interactions, population dynamics, factors affecting these interactions, nutrients and nutrients cycling. Environmental microbiology and biotechnology (22 hours) Environmental pollution; micro-organisms in the polluted environments; metabolic adaptations of such microorganisms; principles underlying the application of microorganisms to ameliorate the pollutants and /or their effects; bioremediation. Sewage and wastewater microbiology; activated-sludge reactors and their designs; application of activated-sludge technology to treat nutrient rich wastewaters. Production of biofuels (bioethanol, biodiesel, biogas): raw materials; micro-organisms and bio-energy production; process biochemistry and limitations; considerations for development of biofuel industry; high rate anaerobic bioreactor systems; biofuel upgrading; integrated biofuel technology for rural development; carbon-trade and life cycle assessment. Industrial microbiology and biotechnology (17 hours) Industrial microorganisms and products: growth and product formation in biocatalysis; characteristics of large-scale fermentations; fermentation scale-up; Isolation and characterisation; industrial production of antibiotics: penicillins and tetracyclines; vitamins and amino acids; exopolysaccharides [EPS], surfactants, flocculants, microbial insecticides; microbial bioconversions; enzymes; vinegar; citric acid and other organic compounds; yeast as an agent in fermentation and as food; alcohol and alcoholic beverages; mushrooms as a food source. Practical (30 hours) Mode of delivery This course will be taught by using lectures, practical and coursework assignments. Assessment Assignments reports, tests, practical reports and end of module examination. Their relative contribution to the final grade is shown below: Requirement Contribution Progressive (Practicals and assignments) 20 % Test 20 % Final examination 60 % Total 100 % Reading List LENGELER, J. W., DREWS, G AND SCHLEGEL, H. G (1999). The Biology of the Prokaryotes SINGLETON, P (1997). Bacteria in Biology, Biotechnology and Medicine. HURST, C. J., CRAWFORD, R. L., KNUDSEN, G. R., MCINERNEY, M. J AND STETZENBACH, L. D (2002) Ed. Manual of Environmental Microbiology, 2nd Edition. American Society for Microbiology Press, Washington, DC Course Name: ADVANCED IMMUNOLOGY Course Code: MBC7205 Course Level: 2 Course Credit: 4 CU Brief Course Description This course requires prior knowledge of the basic biological sciences at BSc or equivalent level. The course will teach an understanding of the immunological basis of disease focusing on self-recognition and the role played by the immune system in the containment of infection, cancer surveillance and tissue rejection and the causes and management of immunodeficiencies. Course Objectives At the end of this course learners should be able to Describe lymphocyte development. Describe immune response to infection. Explain tumour development Describe features of tissue compatibility and transplant immunology. Discuss acquired and inborn immunodeficiencies. Course outline Lymphocyte development (2 hours) The course will cover the development of T and B-lymphocytes with emphasis on the negative selection of self-reacting clones and activation of mature peripheral lymphocytes during immune response. Factors that affect this process and diseases arising from deregulation will be highlighted. Immune response to infection (3 hours) A central theme of the course will be the development of specific, adaptive immune response to pathogens and recall of this response in subsequent infections. This will be the basis of a sub-theme, the application of immunological memory in vaccine development and conditions predisposing to autoimmune disease. Tumour development and chemotherapy (2 hours) The course will cover the role of the immune system in recognition of non-self as the basis of surveillance and removal of cancerous cells. Processes that support tumour establishment, the immunosuppressive effects of chemotherapy and their sequelae. Transplant Immunology (2 hours) The course will explain basis of MHC compatibility and tissue matching. The management of transplant rejection and attendant immunosupression. Acquired and inborn immunodeficiencies (3 hours) The basis of inborn immological diseases such as severe combined immunodeficiency (SCID) and the Bruton’s disease will be highlighted. Comparison will be made with the aetiology and management of acquired immunodeficiency such as AIDS. Tumor immunology (3 hours) Tumor as tissues graft, immune surveillance, tumor-associated antigens detected by immune cells (shared tumor antigens & specific tumor antigens) and by antibodies. Human tumor immune responses and escape mechanisms, Immunodiagnosis (use of antibodies to tumor associated molecules for tumor diagnosis) and immunotherapy (specific active immunization with inactivated tumor cells or non-specific stimulation of immune responses with other agents) and passive immunotherapy using monoclonal antibodies. Immunological tolerance and Immune deficiencies hours) (3 Tolerance mechanisms, central thymic tolerance to self, Post-thymic tolerance to selfantigens, B-cell deletion, B-cell-anergy, autoimmunity results into breakdown of toleramce Artificially induced tolerance. Defective B-cell responses due to lack of B-cell function, Defective cell mediated immune response due to failure of T-cell function e.g. SCID, MHC II deficiency, DiGeorge syndrome, secondary immune deficiencies (due to irradiation, malnutrition, drugs or infection e.g. HIV/AIDS) hereditary defects and deficiencies in complement proteins (C5,C6, C7 &C8), defects in oxygen reduction pathways of phagocytes hence inability to generate toxic H2O2 and free radicals). Advances in vaccination (3 hours) Antigens used as vaccines, effectiveness of vaccines (i.e. induction of the right immune response, stability on storage and sufficient immunogenicity); vaccine safety and cost of vaccination, adjuvants; types and adjuvanticity, passive immunization, non-specific immunotherapy by non-specific stimulation or inhibition of certain components of the immune system (e.g. IL-1 inhibitors against severe cerebral malaria), vaccination against cancer, anti-fertility vaccines (e.g. vaccine based on -chain of hCG linked to tetanus or diphtheria toxoid successfully prevented conception. Immunity to bacteria, viruses and fungi (3 hours) Mechanisms of immunity to bacteria, first and second lines of defences to bacterial infection, antibodies against bacterial infection, of phagocytic actions on bacteria. Models of virus infection, innate immune responses to viruses, host defence involving B and T– cells, immune evasion strategies, immunopathology. Immunity to fungi, monocyte/macrophage killing of fungi. Immunity to protozoa and worms (3 hours) Features of parasitic infections, effector of hosts’ resistance mechanisms, importance of T-cell in development of immunity, evasion of host’s immune response mechanisms, immunopathological consequences of parasitic infection, vaccine for protozoan parasites and worms. Immunological Techniques (3 hours) Antibody-antigen interactions, isolation of pure antibodies and subclasses, assay of complement, isolation of lymphocyte populations, effector-cell assays, gene-targeting and transgenic animals. Precipitation in gels, simple radial immunoassays, double immuno-diffusion assays, turbidity and nephelometry, immuno-electruphoresis, electrophoresis in antibody containing media, 2D-immunoelectrophoresis, use of antibodies for purification of antigens. Tutorials (30 hours) Practicals (30 hours) Mode of delivery This course will be taught by using lectures, practicals and coursework assignments and tutorials. Assessment Assessment will be done through coursework assignments and practical reports, which will constitute a progressive score, and end of module examinations. Their contribution to the final grade is shown below: Components Contribution Progressive (Practicals and assignments) 20 % Test 20 % Final examination 60 % Total 100 % Reading list: 1. Cellular and Molecular Immunology (2002) by Abul K Abbas, Andrew H Litchman, Jordan S Pober. WB Saunders. 2. Laboratory Medicine. Test selection and Intepretation. Joan Howanitz, Peter Howanitz. Edited by: P Joane Cornbleet, Ron B Schifman, Lawrence D Petz. Churchill Livingstone. 3. Immunology Fourth Edition (1996) Edited by Ivan Roit, Jonathan Brosoff and David Male. 4. Basic Immunology: Functions and Disorders of the Immune system 2nd Edition (2004) by Abul Abas Saunder An Imprint of Elservier. Course name: Course Code: Course level: Course Credit: NUTRITIONAL BIOCHEMISTRY MBC7206 2 4 CU Brief course description The course will examine (a) the cellular nutrient homeostasis, proliferation and apoptosis. (b) role of nutrients in signal transduction, gene expression, (c) The relationship between nutrients and immune function. Course objectives At the end of the course the learners should be able to: Describe the role of nutrients in cell signalling Describe the regulation of cellular cholesterol, its trafficking and import into the mitochondria Discuss the interaction between nutrients and the human genome Critically assess the effect of nutrients on immune function Course outline Nutrients and cell signalling hours) (10 Vitamin D regulated pathways: impact on cell proliferation, differentiation, and apoptosis, Mechanisms for dietary regulation of nitric oxide synthesis in mammals The roles of thioredoxin reductases in cell signalling, Roles for biotinylation of histones in chromatin structure, Insulin release, signalling, and insulin resistance, Calcium-dependent signalling, Control of intracellular calcium levels, Calcium-mediated signalling of neurons in the physiological and diseased state: role of endoplasmic reticulum Molecular aspects of Nutrition (10 hours) The core concepts in molecular biology will be reviewed, Effects of genetic code on the response to nutrients (Gly972 Arg Polymorphism in insulin receptor substrate, tafft acids and gene expression,), Nutrient regulation of gene expression (nutrient regulation of gene transcription, transcription factors, translation and post translation) Nutrition and immunity hours) (10 Overview of the immune system, impact of infection on nutrient status, malnutrition and immune function, influence of individual micronutrients on immune function, amino acids, fat and immune function. Tutorials hours) Practical hours) (30 (30 Mode of delivery This course will be taught by using Lectures, tutorials and assignments. Assessment Assignments reports, tests, practical reports and end of module examination. Their relative contribution to the final grade is shown below: Requirement Progressive (Practicals and assignments) Test Final Examination Total Contribution 20% 20% 60% 100% Reading list 1. Michael J. Gibney, Ian A. Macdonald and Hellen M. Roche (2003) Nutrition and Metabolism 2. Garrow JD, James WPT, Ralph A (2000). Human nutrition & Dietetics. 10th Ed.Churchill Livingstone. 3. Anderson RE. (2003). Obesity: Aetiology, assessment, treatment and prevention. Champaign, Illinois: Human Kinetics. 4. Berdanier CD (1996). Nutrient regulation of Gene expression: Clinical aspects, Boca Raton, FL: CRC Press. Course Name: RESEARCH ETHICS AND METHODS Course code: MBC7301 Course Level: Course Credit: 2 U Brief Course Description This course begins with introduction to research planning, experimental designs and scientific research methods, data sourcing, introduction to proposal writing, social research methods, ethical clearance, biosafety and intellectual property rights (IP). Finally the course ends with IP management tools involving different types of agreements and standard elements of agreements. Course Objectives At the end of this course students should be able to: Plan and design experiment Select appropriate research methods for the experiments designed Source appropriate data pertaining to the research envisaged. Write up a research proposal. Explain the use of social research methods. Describe the need for research ethics, clearance, biosafety regulations and intellectual property rights Course outline Concept of research planning hours) (2 Define the concept of research planning, the need for project identification involving. Site selection, study design followed by team selection (multidisciplinary/collaborative). Experimental design, scientific methods and data sourcing (5 hour) Concept of experimental design and scientific methods are explained. Data sourcing from available databases, which involve libraries and websites. Introduction to proposal writing (7 hour) This to include topic selection, literature review, time management, fieldwork where appropriate, problem statement, rationale, justification, hypothesis, objectives and sample size. Social research methods involving structured interviews, participatory rural appraisal, questionnaires and focus discussion. Legal, ethical and bio-safety aspects of research (10 hours) Introduction to fundamentals ethical principles, the current major international guidelines (Nuremberg Code, Helsinki Declaration, Belmont Report, CIOMS & WHO). Ethical review boards, informed consent standards of care, researcher responsibilities and venerable groups in research. Institutional and National/Country ethical clearance requirements explained and participants’ role in the research. Biosafety concerns to cover regulatory organs (National Biosafety Committee and Institutional Biosafety Committee), Institutional guidelines, laboratory safety and disposal methods and sites. Introduction to intellectual property rights. Tutorials hours) (12 Mode of course delivery This course will be conducted in three main ways i.e. formal lectures, reading assignments/coursework, and participatory discussions/presentations. Assessment End of module examination, tests, assignments reports, and presentations. Their relative contribution to the final grade is shown below: Requirement Contribution Progressive (Practicals and assignments) 20 % Tests 20 % Final examination 60 % Total 100 % Reading List The recommended reading will include but not limited to the following literature. African Malaria Network Trust (Amanet) Basic Research Ethics Course.Web based course, www.amanet-trust.org Essential Biosafety (CD-ROM, 2nd edition): The latest scientific and regulatory information for genetically modified and other novel crops and foods. AGBIOS, Canada Course Name: Course Code: BIO-ANALYTICAL TECHNIQUES MBC 7302 Course Level: 1 Course Credit: 4CU Brief Course Description This introduces students to all the experimental techniques and their corresponding principles used in practical biochemistry and molecular biology. It begins with detailed explanation of theories and principles behind the techniques before practical sessions. Broadly, it comprises separation, analytical, immunological and molecular biological techniques. The techniques of separation include chromatographic methods, filtration, centrifugation, precipitation, and electrophoresis. Furthermore, Analytical techniques consist of spectroscopic, mass spectrometric, radioisotopic and electrochemical techniques. Additionally, immunochemical, Molecular biological, recombinant DNA and genetic analysis techniques are covered. Course Objectives At the end of this course learners should be able to: Explain the principles underlying the various biochemical experimental techniques. Demonstrate practical (hands on) competence (skill) with the various biochemical experimental techniques. Design approaches through integration of the biochemical experimental techniques to contribute to the investigation of scientific new problems in biochemistry Course outline Separation techniques: (8 hours) The concepts of the separation techniques: chromatographic methods, filtration, centrifugation, precipitation, and electrophoresis. Chromatographic techniques comprising. Principles of chromatography Chromatographic performance parameters Liquid chromatography (LPLC and HPLC) Adsorption chromatography Partition chromatography Ion-exchange chromatography Molecular exclusion (gel filtration) chromatography Affinity chromatography Gas– liquid chromatography Thin-layer (planar) chromatography Selection of a chromatographic system Electrophoretic techniques of proteins and nucleic acids, Support media (agarose ,polyacrylamide) Types of electrophoresis Capillary electrophoresis Microchip electrophoresis autoradiography isoelectric focusing separation Analytical methods: (8 hours) The principles of mass spectroscopy, spectrophotometric methods and microspcopy are covered. Spectroscopic techniques comprise Atomic and molecular electronic spectroscopy and Vibrational spectroscopy and electron and nuclear spin orientation in magnetic fields. Atomic and molecular electronic spectroscopy includes X-ray, Ultraviolet and visible light spectroscopy, Spectrofluorimetry, Circular dichoursoism, Turbidimetry nephelometry luminometry and atomic spectroscopy Lasers. Vibrational spectroscopy and electron and nuclear spin orientation in magnetic fields consists of Infrared and Raman spectroscopy Electron spin resonance spectroscopy, Nuclear magnetic resonance spectroscopy. Mass spectrometric techniques comprise Ionisation, Mass analysers, Detectors, Structural information by tandem mass spectrometry Analysing protein complexes. Microscopy principles behind light, electron, phasecontrast and other microscopes are covered. Specialised imaging techniques including Image archiving, presentation, Optical-sectioning Imaging living cells and tissues are covered. Immunological techniques: (7 hours) The principles and techniques concerning production of mono- and polyclonal antibodies, purification and fragmentation of immunoglobulins , Affinity and avidity Labeling of antibodies and various immunoassays are covered. Immunoassays including ELISA’s(sandwich et.c) Immunoprecipitation,Immunofluorescence assays (IFA), Immunochemical use of surface plasmon resonance , immunoelectrophoresis(1D and 2D ) , Western blotting, flow cytometry , radioimmunology, Immunohistology, immunosorption.. and dot immunobinding assay. Immobilization of antibodies, and immunohistochemistry, image processing Protein A, T-gel, HOURSP, biotinavidin are covered Molecular biology, recombinant DNA and genetic analysis (7 hours) The manipulation of nucleic acids: basic tools and techniques: Isolation and separation of nucleic acids, molecular biology and bioinformatics, Molecular analysis of nucleic acid sequences, The polymerase chain reaction (PCR), Northern Blotting, Southern Blotting, RFLPs. Nucleotide sequencing of DNA, Constructing gene libraries, Cloning vectors, Hybridisation and gene probes. Screening gene libraries, Applications of gene cloning, Expression of foreign genes. Analysing genes and gene expression, Pharmacogenomics. Analysing whole genomes Tutorials (20 hours) Practicals (40 hours) Mode of course delivery This course will be conducted in three main ways involving Formal Lectures, Practical sessions, Coursework, and Presentations. Assessment Assessment will be by examination, tests, assignments reports, presentations. Their relative contribution to the final grade is shown below: and Requirement Contribution Progressive (Practicals and assignments) 20 % Tests 20 % Final examination 60 % Total 100 % Reading List 1. - Keith Wilson and John Walker (2000) Principles and Techniques of Practical Biochemistry, Fifth Edition, Cambridge University. 2. John F. Robyt and Bernard J. White (1990) Biochemical Techniques: Theory and Practice Waveland Press 3. Keith Wilson and John Walker (1999)6th Edition Principles and Techniques of Biochemistry and Molecular Biology, Cambridge University Press 4 Alfred Pingoud , Claus Urbanke , Jim Hoggett ,Albert Jeltsch (2002)Biochemical Methods: A Concise Guide for Students and Researchers Wiley-VCH Elective Courses: The course requires the completion of one elective course from the following four choices. Students then proceed to develop a research project in a speciality within this field. Course Name: SEMINAR SERIES I Course Code: MBC 7303 Course Level: 1 Course Credit: 2CU Brief Course Description Seminar topics focussing on the process of proposal development, identification of research problems, designing conceptual and theoretical frameworks for studying a research problem. Skills in literature review and synthesis, report writing, and proposal presentations for group review by peers and supervisors. Course Objectives At the end of this course learners should be able to: Identify a research problem Design a scientific framework for studying the problem identified Review and synthesise the available literature Write a scientific proposal Course Name: Course Code: SEMINAR SERIES II MBC 7401 Course Level: 1 Course Credit: 2CU Brief Course Description Seminar topics focussing on their research problems shall be presented regularly during the course of the research process. Literature reviews shall be presented in addition to partial results obtained from their research work. Progress of students’ research and understanding of the conceptual frameworks shall be regularly presented and discussed. Course Objectives At the end of this course learners should be able to: Demonstrate a clear understanding of the research problem Analyse and communicate their findings RESEARCH PROJECTS Brief description Research projects are undertaken in the second year of the Masters Programme. Students can select a research topic of their interest according to the elective courses done in the second semester of their first year. Objectives of research projects At the end of the research project the learners should be able to: Demonstrate ability to design projects Write proposals of acceptable standard Demonstrate their ability to use theoretical knowledge to develop and execute scientifically sound projects Mode of delivery Research projects are undertaken according to the general guidelines and requirements of the graduate school. Students are supposed to write a proposal, which has to be approved before they can commence their research projects. MAKERERE UNIVERSITY DEPARTMENT OF BOTANY MASTER OF SCIENCE IN BOTANY, REVISED EDITION SEMESTERIZED PROGRAMME NOVEMBER, 2008 TABLE OF CONTENTS PAGE 1. Background 2 2. Objectives 2 3. Justification 2 4. Career opportunities 3 5. Facilities/Resources 3 6. Admission 4 7. Structure of the Programme 4 7.1 Part I: Course Work 5 7.1.1 Summary of Part I Programme 5 7.1.2 Semester II (Areas of Specialization) 5 7.2 Part II Dissertation 7 8. Examination Regulations 7 8.1 Progressive Assessment 7 8.2 Course Work Examination Scheme 8 8.2.1 Grading System 8 8.2.2 Progression 8 8.2.3 Re-taking a Course 9 8.3 Part II: Research and Dissertation 9 9. Award of the Degree 9 10. Detailed Syllabuses 10 MAKERERE UNIVERSITY DEPARTMENT OF BOTANY REVISED PROGRAMME FOR THE DEGREE OF MASTER OF SCIENCE IN BOTANY BY COURSEWORK AND DISSERTATION 1. BACKGROUND The Department of Botany has had an M.Sc programme by course work and dissertation since 1994. The Coursework is taught in two semesters of 15 weeks each. During Semester I, core or cross-cutting courses are taught. The core courses are taught jointly with the Department of Zoology to avoid duplication of courses by sister Departments in the same Faculty. In Semester II, elective courses are taught for the purpose of introducing students to various areas of specializations. This programme was revised to take into account the emerging challenges, needs and opportunities in plant resource use and biodiversity conservation. More advanced knowledge of plant science is needed these days of great concern about depletion of biodiversity and global warming. In any case, plants constitute the basis of life on earth and are the livelihood of a great number of people all over the world. The Department of Botany has produced a good number of higher degree graduates who have been absorbed in both the public and private sectors. The Department of Botany has therefore taken up the challenge to contribute towards the production of the critical mass of high caliber crop of plant scientists to meet the changing and increasing needs of the country for plant resources. 2. OBJECTIVES OF THE PROGRAMME The main objective of this programme is to produce graduates with more advanced knowledge and research skills in various disciplines of plant science which are relevant to scientific development and conservation of plant diversity for socio-economic development of the country Specific Objectives After completion of the MSc degree programme, the graduate should be able to (i) (ii) (iii) (iv) (v) Identify research problems; formulate testable objectives, develop appropriate methods and experimental designs and write up viable research proposals in plant science. Implement research projects and produce credible reports Contribute towards finding of solutions for societal problems associated with plant science and resources. Apply knowledge of plant science in development of plant products Apply knowledge of plant science in the restoration of degraded environments (vi) Influence and guide government policy on the sustainable utilization of plant resources through research work and dissemination of knowledge. (vii) Design and implement projects related to plant resources which are geared towards poverty alleviation (viii) Monitor and evaluate research projects concerning ecology, improvement, utilization and conservation plant resources. (ix) Train others in various aspects of pure and applied Botany in the identification of practical problems and the acquisition of practical skills and techniques for handling issues concerned with plant resources 3. JUSTIFICATION There is a great need for M.Sc holders in Botany to fill up vacant posts of lectureships in Departments of Botany (Biology) in Uganda and elsewhere. The graduates are also on demand at National Research Institutes like Kawanda, Kabanyolo, Namulonge, Serere, Uganda Virus Research Institute among others to work on aspects like crop diseases, plant breeding, plant tissue culture, biotechnology etc. Microbiologists are also needed in hospitals, industries like breweries. The programme will produce the above manpower at a cheaper cost than training abroad. The programme will also produce the required personnel to carry out studies like plant inventories, ecology of forests, savanna and wetlands. Such data is required by environmental conservationists and in other sectors of the economy like livestock industry, arable farming and wildlife. Graduates will also be important in carrying out applied botanical research, for example study of economic and social characteristics of indigenous plants that like the medicinal and food plants where currently more attention is being paid. Highly trained manpower in fresh water primary productivity will be produced and their contribution in the development of the fisheries industry is highly important. 4. CAREER OPPORTUNITIES After graduation at postgraduate level, the students find avenues of employment in private sector and government ministries such as Ministry of Agriculture, Animal resources and fisheries, the Ministry of Lands, Water and Environment. Other graduates are employed in research organisations of Natural Research Organisation (NARO), Wildlife Authority, Uganda Forestry Authority, Uganda Wetlands Inspection Division, Non-governmental Organisations (NGOs), The Police and Uganda Peoples Defence Forces (UPDF). Some graduates with Diploma in Education are employed by the Ministry of Education, while others take up postgraduate training in Botany and other related fields such as Forestry and Agriculture. 5. FACILITIES/RESOURCES (i) Funding Sources The past sources of funding for the programme have been the Uganda government, and the linkage between the University Of Vienna, Institute of Plant Physiology (Austria) and the Department of Botany, Makerere University. Other finances have come from the NUFU and NORAD projects funded by the Norwegian government; People and Plants Initiative (WWW/UNESCO/KEW); DAAD, etc. (ii) Physical Facilities The Department of Botany is moderately equipped with research laboratories, microscopes, LCD overhead and slide projectors, video facilities, dark room facilities, chromatography facilities, experimental garden in the Botany Garden (with a greenhouse), two growth rooms, working cold room, incubators, the largest Herbarium in the country, with facilities being continuously improved etc. The Department has a number of computers and has an e-mail connection. (iii) Academic Staff Position The academic staff position in the department is good consisting of two Professors, five Associate Professors, three senior Lecturers, three, several part-time lecturers, two Assistant Lecturers and two Teaching Assistants. In addition to this, in the first semester other resource persons come from the Department of Zoology. The links between the Agricultural University of Norway and the Department of Botany have also involved staff exchange programmes in areas where the department may lack a local person. This has been for short or long periods depending on circumstances. Visiting Lecturers have also come from the Universities of Hull and Southampton, U.K. and from Egypt. 6. ADMISSION REQUIREMENTS Candidates with at least a lower second degree in Botany, Botany combined with Zoology or any other relevant subjects like Geography, Chemistry, Forestry, Agriculture (crop science) and Environmental Science from a recognized University may be admitted to the course. Eligible candidates may be government or privately sponsored. 6.1 Student Capacity The optimum number of students per year is 25 (5 students per each area of specialization i.e. Genetics (Molecular Biology); Natural Resources Ecology and Conservation; Plant Taxonomy & Biosystematics; Microbiology & Plant Pathology; and Plant Physiology). 7. STRUCTURE OF THE PROGRAMME (i) The programme shall run on a semester system consisting of four semesters. Each semester shall last 17 weeks, 15 of which are for instruction (lectures/tutorials/seminars/practicals), etc. One week is for registration and (ii) (iii) the other for examinations. Each course will be divided into course units. A course unit is defined as one contact hour per week per semester. One hour of lecture, tutorial or seminar is one contact hour; two hours of practical or field work are equivalent to one contact hour. Students will be required to have both theoretical and practical experiences during all the course units. The Curriculum shall be divided into two parts. Semesters 1I and II will constitute the first year (Part I). At the end of Semester II there is a Recess Term during which students will finalize the preparation of research proposals. The third and fourth semesters will form the second year (Part II) in which candidates will do research and submit dissertations at the end of the year. 7.1 Part I. Course Work: These are done in the first semester of first year. These courses are offered jointly with the Department of Zoology. 7.1.1: Summary of Part A Programme SEMESTER I CORE COURSES No. i. ii. iii. iv. v. Course Code and Title BOZ7101: Acquisition, Processing and Analysis of Data BOZ7102: Key Aspects of Uganda’s Environment: Climate and Living Resources BOZ 7103: Field Course BOT 7104: Natural Resources Law BOZ 7105: Communication Skills TOTAL LH 30 PH 30 CH 45 CU 3 25 10 30 2 45 30 130 60 100 30 45 30 180 2 3 2 12 7.1.2: SEMESTER II (ELECTIVE COURSES) Summary of Part B Programme A student will be required to choose only one area of specialization elective course from the following: (i) (ii) (iii) (iv) Molecular Biology and Genetics Natural Resources Ecology and Conservation Plant Taxonomy and Biosystematics Mycology, Seed and Plant Pathology (v) Advanced Plant Physiology It is planned that students who are deficient or have interests in some special field may be required to take collateral courses to strengthen their standing. These may include Plant Breeding, Plant Stress Physiology, Cell Biology, Plant Biotechnology and Plant Cell and Tissue Culture. These courses are normally offered in the Department of Botany as some of the final year options at undergraduate level B1: MOLECULAR BIOLOGY AND GENETICS No. i. ii. iii iv. v. vi. Course Code and Title BOT7201: Cell and Molecular Genetics BOT7202: Genetic Engineering BOT7203: Cytogenetics BOT7204: Population & Evolutionary Genetics BOT7205: Advanced Plant Molecular Biology BOT7206: Ecological Genetics TOTAL LH 20 20 20 30 20 20 125 PH 20 20 20 30 20 20 170 CH 30 30 30 45 30 30 210 CU 2 2 2 3 2 2 13 B.2 NATURAL RESOURCES ECOLOGY & CONSERVATION No. i. ii. iii. iv. v. vi. Course Code and Title BOT7207: Renewable Natural Resources Ecology BOT7208: Rangeland Ecology and Management BOT7209: Tropical Rain Forest as Climax Vegetation BOT7210: Natural Resource Conservation and Management BOT7211: Island Biogeography for Conservation Biology BOT7212: Research, Experimentation and Survey Sampling TOTAL LH 20 20 25 PH 20 20 10 CH 30 30 30 CU 2 2 2 25 10 30 2 30 - 30 2 20 20 30 2 140 80 180 12 LH 30 PH - CH 30 CU 2 20 25 35 20 30 50 10 20 20 30 45 30 45 30 45 3 2 3 2 3 B.3 PLANT TAXONOMY & BIOSYSTEMATICS No. i. ii. iii iv. v. vi. Course Code and Title BOT7213: Development and Systems of Classification BOT7214: Classification and Identification Methods BOT7215: Nomenclature: The Taxonomic Structure BOT7216: Elements of Applied Plant Taxonomy BOT7217: Taxonomic Techniques and Facilities BOT7218: Field and Herbarium Techniques TOTALS 170 110 225 15 LH PH CH CU 35 20 45 3 20 20 20 35 15 20 20 20 20 30 30 30 30 45 30 2 2 2 3 2 B.4 MICROBIOLOGY AND PLANT PATHOLOGY Course No. Course Titles BOT 7219 Biology and Systematics of Fungi and Bacteria. Principles of Plant Pathology The Physiology of Fungi and Bacteria. Advanced Plant Virology. Applied Microbiology and Biosafety. Compendium of Diseases of major Crops in Uganda and Crop Epidemics. Food Microbiology. Total BOT 7220 BOT 7221 BOT 7222 BOT 7223 BOT 7224 BOT 7225 30 30 45 3 165 160 270 17 B.5 PLANT PHYSIOLOGY No. i. ii. iii. iv. v. vi. vii Course Code and Title BOT 7226: Mineral Nutrition in Plants BOT 7227: Plant Water Relations BOT 7228: Stress Physiology and Phytoremediation BOT 7229: Plant Growth and Development BOT 7230: Physiology of Growth and Crop Yield BOT 7231: Plant Energetics BOT 7232 Plant Tissue Culture TOTALS LH 20 20 20 20 20 20 20 140 PH 20 20 20 20 20 20 20 140 CH 30 30 30 30 30 30 20 210 CU 2 2 2 2 2 2 2 14 NOTE: A detailed syllabus of the courses (7101 – 7230) is attached in Section 9. 7.2 YEAR TWO: DISSERTATION Activity Period Production of Research Proposal Research and Production of Dissertation Recess Term of Year 1 Semesters III & IV 8. EXAMINATION REGULATIONS 8.1 Progressive Assessment (PA) Progressive Assessment shall consist of test(s), practicals, assignments, seminar presentation, etc. done during the courses. Progressive Assessment will contribute 40% of the total mark for each course unit. 8.2 Course Work Examination Scheme At the end of each semester there will be a written examination in each of the courses offered during the semester. The examination score will contribute 60% (and PA 40%) of the final mark for the course. A pass mark in any course is of at least 50%. 8.2.1 Grading System (a) Each course shall be graded out of a maximum of 100 marks and assigned appropriate letter grades and grade point average as follows: Marks (%) 80-100 75-79.9 70-74.9 65-69.9 60-64.9 55-59.9 50-54.9 45-49.5 40-44.9 35-39.9 Below 35 Letter Grade A B+ B BC+ C CD+ D DE Grade Point 5.0 4.5 4.0 3.5 3.0 2.5 2.0 1.5 1.0 0.5 0 (b) These additional letters shall be used where appropriate: (c) The pass grade point per course shall be 2.0 8.2.2 Progression Through the Course Progression through the course shall be assessed in there ways: (a) Normal Progression This occurs when a student passes all courses taken. (b) Probationary This is a warning stage and occurs if: (i) a student fails a core/compulsory course, or (ii) a student obtains a grade point average (GPA) or a cumulative grade point average (CGPA) of less than 2.0 Probation is removed when either of the conditions in (a) or (b) no longer holds. (c) Discontinuation A student shall be discontinued from the programme if one of the following conditions obtains: (i) (ii) Receiving to probations on the same core/compulsory course. Receiving two consecutive probations based on GPA or CGPA. 8.2.3 Re-taking a Course There shall be no supplementary examinations in any course of the programme. However a student may retake any course when it is offered again in order to: (a) pass it if the student had failed it before (b) improve the grade if the first pass grade was low A student who does not wish to retake a failed optional course shall be allowed to take a substitute optional course. 8.3 Part II: Research and Dissertation (i) (ii) (iii) (iv) (v) (vi) This comprises of an acceptable research project of original nature based on the relevant areas of specialization leading to the submission of a dissertation. Both the research proposal and dissertation must conform to the University regulations. The research project will be undertaken in the second year of the programme (the third and fourth semesters). A candidate shall be required to develop/produce and present an acceptable research proposal during the Recess Term. A candidate must do the research and work on the dissertation in Semesters III and IV and submit the dissertation at the end of the Semester IV. The dissertation shall be marked pass or fail. A candidate with a dissertation requiring minor corrections will be required to resubmit a corrected version within 3 months. A candidate with a failed dissertation will be required to resubmit within 6 months. 9. AWARD OF THE DEGREE The degree of Master of Science in Botany shall be awarded to a candidate who successfully completes all parts of the course. The degree of Master of Science in Botany shall be awarded without classification; however the Academic Transcript shall be graded as follows: A = 80-100% B+ = 75-79.9 B = 70-74.9 B- = 65-69.9 C+ = 60-64.9 C = 55-59.9 C- 0-49.9 D+ = 45-49% (Compensatable Fail) F = 0-44% (Fail) 10. DETAILED SYLLABUSES 10.1 Part I (i) First Semester (Compulsory courses): BOZ. 7101 Acquisition, Processing and Analysis of Data (3 CU) Course Objectives • At the end of the course, the student should be able to: • Justify the importance of statistics in biological studies. • Apply skills of computer-based packages for statistical analyses. • Explain the various experimental designs in biological studies. • Develop proposals, collect data, analyse it and write a report. Course outline Source of Data; Libraries, Herbarium, Museums, National and International information services. The planning of research project. Experimental design and the scientific method Organization and collection of data. Types of scientific calculators and computers. Introduction to computing. Introduction to data analysis. Information handling, storage, and retrieval. Probability. Type of statistical analysis, their uses and applications. Library programmes e.g. Linear programming. Presentation of data in graphical and tabular forms. Interpretation of results. Preparation of scientific reports. BOZ. 7102 Key aspects of Uganda’s Environment: Climate and Living Resources (2 CU) Course Objectives • At the end of the course, the student should be able to: • Relate physical environment to biological communities. • Interpret climatologically related data. • Outline main characteristics of major biological resources in East Africa. • Demonstrate the skills in planning and implementing conservation activities. Course outline Introduction to climate of Africa. Introduction to tropical convergence zone and factors affecting its behaviour. Climate measurements; microclimates. The hydrological cycle. Major biological resources; traditional impacts. Introduction to current human impacts. Conservation and Management of Natural Resources. BOZ. 7103 Field Course (2 CU) Course Objectives • At the end of the course, the student should be able to: • Justify the importance of measuring and analysing biodiversity. • Apply biodiversity research methods. • Explain the various experimental designs in biological studies. • Develop proposals, collect data, analyse it and write a report. • Demonstrate the use of a variety of field research equipment and materials. • Conduct field research projects Course outline A two week programme of visits to a variety of places designed to illustrate aspects of course work. Some field techniques will be introduced, including photography and survey. BOT 7104 Natural Resources Law (2 CU) Course Objectives • At the end of the course, the student should be able to: • Justify the importance of appropriate policies and laws in biodiversity management. • Explain the various conservation related international and national policies and legislation. • Interpret biodiversity related policies and laws. • Relate theory of Intellectual property Rights regimes to natural resources. Course outline National vs International law and natural resources. The court system, adversarial system of legal process and problems in defence of the environment. The role of law in ecosystem protection (regulatory standard setting/enabling conflict resolution). State intervention or public trust. Relevant substantive and common law in Uganda and their relevance to plant ecological protection. Legislative deficiencies and stop gap administrative measures. Contemporary legal concepts, impact assessment, polluter pays principal etc. International law enforcement mechanisms, relevance and short comings as natural resources regulatory system. territorial plant protection, general concerns and relevance (customary and treaty based.) State obligation arising thereunder. BOZ 7105 Science Communication Skills (2 CU) Course Objectives • At the end of the course, the student should be able to: • Outline the basic elements of communication skills.. • Explain the importance of effective communication of scientific information. • Identify and use appropriate techniques and skills for developing information for oral and written presentations. Course outline What is scientific research? The scientific method. Writing objectives. The art of scientific investigation: preparation, experimentation, chance, hypothesis, imagination, intuition, reason, observation, difficulties, strategy. Writing technical reports. Writing a scientific paper. Writing a research proposal. Writing a review of a report/paper book. Literature search/internet. Presenting a paper at a conference. Editing a book/paper 10.2 PART I (ii) Second Semester: Areas of Specialisation Students are expected to select only ONE of the following areas of specialization (B.1, B.2, B.3, B.4 and B.5). All courses in any one area of specialization are core courses. B.1: MOLECULAR BIOLOGY AND GENETICS BOT 7201 Cell and Molecular Genetics (2 SCH) Course objectives: At the end of this course the student should be able to: Describe the structure and function of DNA Describe and explain the steps involved in a PCR reaction Outline the principles underlying the DNA sequencing technique Describe the process of DNA replication, transcription and translation in eukaryotes and prokaryotes Compare and contrast the Lac and Trp operons Course outline: Physical and chemical structure of DNA i.e. base pairing and double helix, base composition, forms of helices and size of molecules. Denaturation and melting curves. The polymerase chain reaction and its applications, DNA replication – mechanisms, enzymology, initiation and terminations. Hydrogen bonding and hydrophobic interactions. Base stacking. Principles of DNA and protein sequencing; Replication in pro- and eukaryotes. Transcription and processing of RNA molecules, Lac and Trp operons. Translation, elongation and termination. Genetic code and decoding system, regulation of the gene expression in pro-and eukaryotes operon function. BOT 7202 Genetic Engineering (2 CU) Course objectives: At the end of this course the student should be able to: Describe the different methods of gene transfer in plants Discusss the applications of recombinant DNA technology in plant science Describe techniques of identification of desired genes in a cloned library Course outline: Primary objectives of genetic engineering in plant science; The concept of a transgene; Genetic engineering and agricultural development in the developing world; Recombinant DNA techniques; Tools and techniques in genetic engineering; Plasmid, cosmids, YAC, and viral vectors; Molecular cloning; Genomic and cDNA libraries; Methods of gene transfer in plants; Plant genetic transformation; Selection of candidate genes for genetic engineering in plants; Construction of transgenic plants; Assessment of environmental impact of transgenics; Bio-ethics, Bio-safety, and Bio-Policies; Role of genetic engineering and food security. BOT 7203 Cytogenetics (2 SCH) Course objectives: At the end of this course the student should be able to: Describe the hierarchical organization of DNA into chromosomes Discuss the evolutionary implications of chromosome structural changes Describe the causes and consequences of chromosome numerical changes within cells Explain the significance of chromosome fusion and fission in karyotype evolution Course outline: Organization of genetic material into Chromosomes – histones, chromatin, nucleosomes, etc. Polytene and Lumpbrush chromosomes. Structural changes in chromosomes: duplications and deficiencies, inversions and interchanges with emphasis on their evolutionary significance and their applications in gene-mapping. Chromosome numerical variation – aneuploidy, euploidy, evolutionary significance and induction of autopolyploidy and allopolyploidy. Applications of polyploidy. B-chromosomes. Chromosome structural variation – Fusion, fission, inversions and interchanges. Karyotype evolution (both quality and quantity). Meiosis – Synaptonemal complexes and recombination modules. Developmental genetics of Drosophila. BOT 7204 Population and Evolutionary Genetics (3 CU) Course objectives: At the end of this course the student should be able to: Explain the Hardy – Weinberg Principle Discuss factors that change gene frequencies in populations Describe techniques used to quantify genetic variation within and between populations Outline evidences for evolution Discuss contemporary examples of evolution in action through natural section Course outline: The behavior of genes in populations; the Hardy-Weinberg Principle; Factors that affect the Hardy-Weinberg equilibrium; Types of equilibrium, genetic drift, founder effect, equilibrium between determinate and stochastic forces. Partitioning of variance, measuring heritability, response to selection, genotype – environment interactions. Avoidance of inbreeding theories of evolution – mutationism, creationism, Darwinism, Lamarckism, Neodarwinism, Evidences for evolution. Sources and measurement of genetic variation – visible mutants, lethals, isozymes, chromosome variation, variation of nucleic acid sequences, transposable elements. Industrial Melanism, insecticide resistance, heavy metal tolerance. BOT 7205: Advanced Plant Molecular Biology (2CU) Course objectives: At the end of this course the student should be able to: Discuss different approaches of studying gene function in plants Explain molecular mechanisms of plant develpoment and defenses Do a comparative analysis of the RNA and DNA worlds in plant cells Propose an executable molecular biology experiment Course outline A review of the central theme of molecular biology; Model organisms for plant molecular biology; Plant genomes, transcriptomes, proteomes and metabolomes; Plant genomics and systems biology; Reverse and forward genetics; Mapping; Plant nuclear and organelle gene structure, function, and expression; Regulation of gene expression in plants; Reproductive and vegetative development; Molecular defence of plants to biotic and abiotic stresses; Innate immunity in plants; Light and hormone signalling The small RNA world, RNA silencing, and suppression of RNA silencing in plants; Applications of RNA silencing in plant molecular biology; DNA and protein bioinformatics tools; Techniques in plant molecular biology. Experimental design in plant molecular biology. BOT 7206 Ecological Genetics (2 SCH) Course objectives: At the end of this course the student should be able to: Explain sources of polymorphism within populations Explain the differences between Batesian and Mullerian mimicry Differentiate between allopatric, sympatric, parapatric and peripatric speciation Discuss the merits and demerits of the different concepts of a species Course outline: Polymorphism – Mimicry, color forms of Papilio dardnus and Acrea encedon. Automimicry, chemical mimicry, Batesian and Mullerian mimicry. Industrial melanism. Insecticide resistance Heavy metal tolerance. Eb2HbA alleles, Anaemia, Malaria. Speciation, extinction and genetic conservation. Species criteria, reproductive isolation clines, hybrid zones, mechanisms of speciation. B.2: NATURAL RESOURCES ECOLOGY AND CONSERVATION BOT 7207 Renewal Natural Resources Ecology (2 CU) Course Description The course introduces the learner to the historical development of Ecology as a discipline. It will also relate the basic principles of ecology to the management of the natural resources especially the major ecosystems’ renewable resources. Course Objectives • At the end of the course, the student should be able to: • Outline the roots, development and application in Ecology. • Explain the types of natural resources and their appropriate wise use. • Explain the role of disturbance in ecological systems • Explain the possible causes, consequences and remedies to environmental pollution • Discuss the conservation and management of the resources. Course outline Introduction and definition of ecology, The roots of ecology, historical development of ecology, the application of ecology, Basic principles, problems and approaches in ecology. Introduction to the science of natural resources. Disturbance ecology; Successions and Regeneration. Major terrestrial ecosystem of the world grasslands, savanna and woodlands, shrublands, forests, tundras, deserts and wetlands: location, soils and climate, type structure and function. Toxic effects of environmental pollutants. BOT 7208 Rangeland Ecology and Management (2 CU) Course Description The course introduces the learner to the types and major characteristics of wildlife habitats. It will also relate the application of principles of ecology to the management of the wildlife habitats. Course Objectives • At the end of the course, the student should be able to: • Outline the types and characteristics of wildlife habitats. • Explain the methods of and problems in the conservation of wildlife in their habitats. • Describe the importance of these resources and relate them to the social, economic and ecological development. • Discuss the rangelands and wildlife as renewable natural resources. Course outline Wildlife habitats: important ecological principles; size of an area, flow of energy, ecosystems concept, plant succession, stability and resilience of ecosystems, irregular events and carrying capacity. Habitat edges and ecotones. Habitat analysis and evaluation. Habitat improvement techniques. Fire ecology; fire and human civilization, types and behaviour of fires. Plant/fire/animal interactions. Effects of fire on habitats in different environments. Use of fire in natural resources management. Effects of grazing and management on grasslands. Desertification. Socio-economic and cultural values of rangelands. BOT 7209 The Tropical Rain Forest as Climax Vegetation (3 CU) Course Description The course introduces the learner to the types and major characteristics of tropical rain forests. It will also relate their ecology to the utilization and management. Course Objectives • At the end of the course, the student should be able to: • Classify and define the various tropical rainforests climaxes. • Describe the structure, dynamics and functions of tropical rainforests. • Discuss the tropical rainforests’ values and benefits. • Appreciate the causes and problems of deforestation. Course outline Climatic, biotic and abiotic climaxes, tropical rain forest composition, life forms and tree populations. Profile diagrams and variation. Forest dynamics: phenology and seasonality. Tree growth rates, longevity and mortality. Deforestation problems; types and levels of disturbances; recruitment, regeneration and recovery; forest succession. The Socio-economic, cultural and ecological values and functions. Ethnobotany and conservation for the people. Wild plant trade: Potentials and problems. BOT 7210 Natural Resource Conservation and Management (3 CU) Course Description The course introduces the learner to the types and major characteristics of natural resources. It will also relate the ecology to their utilization and management. Course Objectives • At the end of the course, the student should be able to: • Relate conservation and management of natural resources. • Explain the objectives of conservation and management of natural resources. • Develop, monitor and evaluate management plans for wildlife resources. • Evaluate damaged ecosystems for restoration, rehabilitation and reallocation. Course outline What is Conservation Biology? Principles of Natural Resources Conservation and Management. Collaborative Resources Management. Conservation and Land Use. Sources of Biotic degradation. Establishment and Management of in situ and ex situ Conservation areas. Conservation related Enterprises. Environmental and Conservation Planning, Environmental auditing. Species Recovery Planning. Ecosystems Rehabilitation and Restoration. BOT 7211 Island Biogeography: Implication to Conservation Biology (3 CU) Course Description The course introduces the learner to the theory of island biogeography and its application to the design of protected. Course Objectives • At the end of the course, the student should be able to: • Discuss the island biogeography and equilibrium theory of Macarthur and Wilson • Relate island biogeography and equilibrium theory to the design of nature reserves. • Advise on the design of the various categories of nature reserves or protected areas. Course outline Types of Botanic Gardens and Herbaria. Role of Botanic Gardens & Herbaria in Plant Resources Conservation and Management. Basic Principles of Landscaping. Establishment and Management Skills of Botanic Garden and Herbaria. Development of Communication strategy and Interpretation Centres. Databases and their Management. Guidelines for the selection of nature reserves. Assessment of biodiversity and site evaluation. Design of nature reserves. BOT 7212 Research, Experimentation and Survey Sampling (2 CU) Course Description The course introduces the learner to the types and major characteristics of natural resources. The course will further introduce the learner to the theory and practice of the laboratory and field methods in ecological. It will also relate the ecology to their utilization and management. Course Objectives • At the end of the course, the student should be able to: • Justify the importance of measuring and analysing biodiversity. • Apply biodiversity research methods. • Explain the various experimental designs in biological studies. • Develop proposals, collect data, analyse it and write a report. • Demonstrate the use of a variety of field research equipment and materials. • Conduct field research projects Course outline Introduction to ecological survey sampling. Experimental vs descriptive research. Stages of research experiment. Basic terminology in survey sampling. Problem analysis and statement. Some basic concepts in research and monitoring. Sampling survey designs. Types of surveys. Some special considerations and problems. Plant survey sampling methods and techniques. Animal census and survey methods and techniques. Revisiting inventory, censuses and indices. Considerations in selecting of methods. Most field practicals will be conducted at MUBFS in Kibale Forest and UIE at Mweya. Possible projects will include forest and rangeland management, damage assessment, forest gap dynamics, forest regeneration evaluation of enrichment of ecosystems and socio-economic analysis of local resource users. Extra field exercises will cover sampling techniques, plant community measurements, development impact assessments and mini-projects; design, executions and write up. B.3 PLANT TAXONOMY AND BIOSYSTEMATICS (16 CU) COURSE OBJECTIVES: By the end of the course students should be able to: Discuss the development of and identify the various systems of classification Discuss the concept of and the various types of characters Construct, use and discuss the various types of identification keys Discuss the principles of nomenclature, differentiate the various types and analyze problems of nomenclature of cultivated plants and hybrids Assess the ethnobotanical methods and outline the systems and practices of African traditional medicine; identify the native and introduced poisonous plants. Survey and discuss the various types of taxonomic evidence Describe the acquisition of data including field and herbarium data and demonstrate the analysis of taxonomic data Carry out a scientific plant collection and curate the collections BOT 7213: Development and Systems of Classification (2 CU) Course objectives: By the end of the course students should be able to: (i) Discuss the development of and identify the various systems of classification (ii) Compare and contrast Pre- and Evolutionary classifications (iii) Discuss the principles of speciation Course Outline Pre-evolutionary classifications: Ancient classifications; The herbalists; Linnaeus and his apostles. Evolutionary classifications (Phylogenetic systems): Engler & Prantl; Bessey; Hutchinson, Cronquist and Takhtajans’s systems. Effects of evolutionary theory on systematics, including evolution and the differentiation of species. Abrupt and gradual speciation. Species, races and isolating mechanisms like geographical, ecological, seasonal, temporal and mechanical isolation; Internal barriers. Delimitation of taxa showing evolutionary patterns; comparability of species and higher taxa and use of intraspecific ranks. BOT 7214: Classification and Identification Methods (3 CU) Course objectives: By the end of the course students should be able to: (i) (ii) (iii) Define character Discuss the concept of and the various types of characters Construct, use and discuss the various types of identification keys Course Outline Introduction to the concept of characters, definition and value of character, analytical and synthetic characters. Qualitative and Quantitative characters;“Good” and “Bad” characters. Weighting of characters. Construction and use of diagnostic identification keys i.e. sequential keys like the computer, edge-punched and body- punched (petyclave) keys. BOT 7215: Nomenclature: The Taxonomic Structure (2 CU) Course objectives: By the end of the course students should be able to: (i) (ii) (iii) (iv) Discuss the principles of nomenclature Define and differentiate the various types of names Outline and analyze the problems of nomenclature of cultivated plants and hybrids Describe the taxanomic categories and groups and their usage Course Outline Typification. Principles of nomenclature. Discussion and application of the international codes; Publication. Principle of priority. Legitimate names; synonyms and orthography. Author citations. Problems of nomenclature of cultivated plants and hybrids. The hierarchical system. Taxanomic categories and groups and their usage with particular reference to species, genera, families and practical examples. BOT 7216: Elements of Applied Plant Taxonomy (3 CU) Course objectives: By the end of the course students should be able to: (i) Assess the ethnobotanical methods especially those relevant to plant taxonomy (ii) Outline the systems and practices of African traditional medicine with particular reference to commonly used medicinal plants Identify the native and introduced poisonous medicinal, poisonous and other categories of useful plants Course Outline General ethnobotany with particular reference to methodology. Systems and practices of African traditional medicines: history of plants in medicine and ancient western medicinal practitioners; ethnosystematics; traditional methods of preparing and dispensing herbal drugs; modern methods of identifying authentic medicinal plants; phytochemical and other methods of testing the active principles of medicinal plants ; drugs from plants (antivirals, antibiotics). Identification of native and introduced poisonous plants; poisonous plants affecting man and domestic animals and their toxic chemical constituents; methods of controlling poisonous plants. Other categories of useful plants: food; fibres; plants used in making handcrafts; etc. BOT 7217: Taxonomic Techniques and Facilities (2 CU) Course objectives: By the end of the course students should be able to: (i) (ii) (iii) Survey and discuss the various types of taxonomic evidence Discuss the merits and demerits of the various types of taxonomic evidence Describe the acquisition of data including field and herbarium data and demonstrate the analysis of taxonomic data Course Outline Taxonomic evidences: morphology; anatomy; palynology; embryology; cytology; molecular and biochemical systematics; numerical taxonomy; experimental taxonomy; phytogeography. Botanic gardens; herbarium, i.e. size, state, history and development, purposes, sources of materials etc. Library. Character variation; data accumulation and analysis. Data presentation and documentation including use of dendograms, phenograms, histograms and interpretation of results. BOT 7218: Field and Herbarium techniques (3 CU) Course objectives: By the end of the course students should be able to: (i) (ii) (iii) Discuss the setting-up and functions of botanic gardens and herbaria Carry out a scientific plant collection Curate plant collections Course Outline Field and collecting expeditions. Flora inventory procedures and processes. Herbarium practices emphasizing the making of a herbarium and the techniques on preparation and curating of specimens. The making and organizing of botanic gardens. Students will also be required to write essays on selected topics as a training in searching for information in monographs, floras, abstracts, journals, etc. Students will carry out individual projects on selected taxa (preferably a genus) and will also be introduced to writing project reports. For the taxon selected they will be required to consider among other things: Exomorphology, Endomorphology (Anatomy) Chemotaxonomy, Palynology and Numerical taxonomy. B.4 MICROBIOLOGY AND PLANT PATHOLOGY Course objectives 1. To offer a strong science foundation for advanced learners in courses of biology of microorganisms, microbial diversity, pathogenicity and food microbiology. 2. To highlight ecological concerns impacting food production and the environment. 3. To train competent scientists in the fields of Microbiology in the areas of medical, agricultural, food, industrial, microbial genetics or physiology. 4. To furnish necessary experience in academic and practical skills in pure or applied biological sciences. BOT 7219 Principles of Plant Pathology (3CU) Diversity of plant pathogens; Plant pathogens as a threat to food security; The significance of plant pathology; The disease triangle; Plant pathology in the tropical climate; Plant disease and climate change; Characteristics plant diseases due to different pathogens; General principles of plant disease etiology; Nature of pathogenesis; The dynamic survival game between plants and their pathogens; Diversity of host plant- pathogen interactions; Plant disease epidemiology; General principles of plant disease control; Non-parasitic diseases of plants; Strategies and practices for integrated disease control; Plant protection and quarantine. BOT 7220: Biology and Systematics of Fungi and Bacteria ((2CU) Phyllum Chytridiomycota: The vegetative body, modes of reproduction and the morphology and behaviour of reproductive structures. Similarities and differences between Order Blastocladiales and Order Monoblepharidales. Phyllum Oomycota (chromista). Formation of sexual and asexual reproductive structure, their morphology and behaviour in the Order Saprolegniales. Why are the Oomycota, not true fungi? The Order Peronosporales: Formation, morphology and behaviour of reproductive structures. The fungi, which causes serious plant diseases (blights and mildews). Phytophthora infestans and Plasmopara viticola in world history. Phylum Ascomycota: The major divisions of Ascomycota, modes of sexual and asexual reproduction. The basic sexual cycle. The morphology of the ascocarp, ascus and ascospores. Brief study of the Order Erysiphales emphasizing the conidial forms and their importance. Detailed study of the Order Eurotiales: The morphology and criteria for the identification of the Aspergilli and Penicillin and their teleomorphs. The importance off the anamorphs to the humans. Order Sordariales covering Sordaria, Podospora, Neurospora and Chaetomium. Order Xylariales – the stromatic pyrenomycetes. The morphology of stomata, the ascocarps, asci and ascospores of Xylaria, Hypoxylon, Daldinia and Rosellinia. Order Hypocreales, Morphology of stromata of genera Hypocrea and Nectria. The anamorphs of the Hypocreales genera and their importance. Class Discomycetes: Occurrence in nature; the general structure of the apothecium, Order Pezizales and the characteristics of operculate forms and Order Helotiales and characteristics of the inoperculate forms. Mention of the hypogean discmycetes. Deuteromycetes: The form, orders in Deuteromycetes and the form families of the Moniliales. The Hyphomycetes and Saccardo’s system of classification of conidial fungi. Phyllum Basidiomycota: The major divisions in the Basidiomycota. The development of mycelium and the basidium. Order Agaricales – criteria for the identification of the mushrooms. Brief treatment of common families. The importance of the Agaricales. Order Aphyllophorales: Identification of the Aphyllophorales. Description of the families. Importance of the Aphyllophorales. Systematics of bacteria Class I: Schizophyceae , (Cyanobacteria). Class II: Schizomycetes (The Eubacteria), Pseudomonadales, Chlamydobacteriales. Hyphomicrobiales, Eubacterials, Caryophanales, Actinomycetales, Begiatoales, Myxobacterials, Spirochaetales, Mycoplasmatales. Class III: Microtatobiales(order Rickettsiales). BOT 7221: The Physiology of Fungi and Bacteria (2CU) Fungi: Dormancy and germination of spores of fungi: The growth of fungi and its definition – increase in cell numbers and linear extension of hyphae – Growth on solid and in liquid media both unicellular and mycelial fungi. Factors which affect fungal growth: Temperature, pH, Nutrients (Carbon, Nitrogen, minerals and vitamin), light, Air, Water. Reproduction of fungi: Asexual and sexual reproduction. Factors which affect reproduction: Light, pH, Nutrients (Carbon, Nitrogen, Minerals, Vitamins), light, Air Water. Mechanisms of spore liberation and spore dispersal. Metabolism and metabolic products of importance to humans: acids, enzymes, antibiotics, mycotoxins etc. Bacteria: Growth of bacteria. Factors that affect bacterial growth. Reproduction in Bacteria. Mechanisms of dispersal of bacteria. Metabolism and metabolic products of importance to humans. BOT 7222: Advanced Plant Virology (2CU) Diversity of plant viruses, viroids, defective [satelite] viruses, and satelite RNAs; Significance of plant virus diseases; Major tropical plant viruses of economic importance; Plant virus taxonomy and contemporary plant virology; Virulence and pathogenicity of plant viruses; Symptoms and aetiology of plant virus diseases; Methods of studying plant viruses; Plant virus diagnostics; Diagnostics Modes of plant virus transmission by vectors; Non-vector mediated transmission of plant of plant viral proteins; Plant virus infection process; viruses; Plant virus ecology and epidemiology; Control strategies of plant virus diseases; Mechanisms of virus movement in plants; Structure of virus particles; Replication of plant RNA viruses; Replication of plant DNA viruses; Genome expression strategies in various plant virus taxonomic groups; Evolutionary dynamics of plant viruses; Similarities between plant and animal viruses; Synergism, competition, complementation and social strife in plant virus world; Mechanisms of host plant resistance to plant viruses; RNA silencing; Virus-induced gene silencing (VIGS) in plants; RNA silencing suppression by plant viruses; Applications of VIGS in plant molecular biology; Plant virology and nanotechnology; Comparative and evolutionary genomics of selected plant virus families. BOT 7223 Applied Microbiology and Biosafety (3 CU) Classical microbial processes; beer and wine making; bread making; the processing of milk to diary products by lactic acid; cheap carbohydrate containing wastes conversion to acetone; butanol, etc. via fermentation; biodegradation. Application of microorganisms in medicine ; agriculture, energy production (biogas; gashol); food product; production of pharmaceuticals; production of antibiotics; steroids; microbial leaching; teaching of industrial effluent in modern genetic techniques. Biosafety: Microorganisms and modes of disease transmission. Standard precautions. Principles of contamination and infection control. Health and hygiene standards. Biological safety cabinets and related devices. General precautions and equipment. Waste management. BOT 7224: COMPEDIUM OF DISEASES OF MAJOR CROPS IN UGANDA AND CROP EPIDEMICS (2CU). Students will be required to compile the Host (Crop), Pathogen, Disease it causes, how the disease causes crop loss and how it is controlled. The crops will include: Bananas, Coffee, Tomatoes, Common beans (Phaseolus) Maize, Sugar canes, Sorghum Cassava, Sweet potatoes, Groundnuts and Cotton. Plant epidemics, conditions which cause their outbreak and conditions which lead to decline epidemics of international importance. BOT 7225: FOOD MICROBIOLOGY (3CU). Microbial flora of fresh foods, Microbial spoilage of foods. Methods of detection of micro-organisms in foods. Methods of preservation of foods. Micro-organisms as Food Single-Cell Protein. Recent developments in procedures used to assay and control the microbiological quality of food and protect public health. Fungi: The occurrence of fungi on crops before harvest, after harvest and during storage. The effects of fungi on fresh food stuffs (fresh fruits, grains and root crops) and stored dry foodstuffs. Methods of detecting toxigenic fungi in food stuffs and feeds. Common toxigenic fungi and the mycotoxins they produce. Occurrence of mycotoxins in food stuffs and feeds. Methods of detecting mycotoxins in contaminated foods and feeds. Prevention of contamination of foods and feeds by mycotoxins. Mycotoxin Survaillance International standards on mycotoxin contamination of food stuffs. B.5 PLANT PHYSIOLOGY BOT 7226 Mineral Nutrition in Plants (3 CU) Course objectives: At the end of the course, the student should be able to: i) ii) iii) iv) v) vi) Review the biogeochemical cycle Describe the mechanism of mineral uptake Describe the availability of mineral nutrients Discuss the roles of mineral nutrients in plants Describe the effect of excess and deficiency of mineral nutrients in plants Identify suitable nutrients types and levels for improving plant production Course outline Soil as a source of mineral nutrients; Mineralization of organic matter; Mechanisms of mineral uptake by plants and vertical transport. Physiological roles of mineral salts (boron, molybdenum, zinc, copper, manganese, iron, chlorine, and calcium) in plants. The roles of other beneficial elements (silicon, sodium, cobalt, nickel, selenium and aluminum) in plants. Effects of deficiency and excess on plants. Salt stress. Determination of soil pH. Metabolism of mineral salts by plants: their incorporation into organic compounds; enzyme activities. Analysis of mineral contents of plants and soils. Application of mineral salts in agriculture as fertilizers, germination, stimulation, herbicides and disease control. . BOT 7227 Plant Water Relations (2 CU) Course objectives At the end of the course, the student should be able to: Describe the physical and properties of water Relate the physiological functions in plants to the physical and chemical properties of water Outline the mechanisms of water uptake and distribution in plants Relate the hydrological cycles to the global distribution of vegetations Apply the knowledge of phloem transport to primary productivity Physical and chemical properties of water and their physiological effects on plant growth and development. Water uptake and its transportation in plants. Transport of materials in water within plants; apoplast, symplast and plasmodesmata. Types of soil water and its availability to plants. Water & global distribution of vegetation. Phloem transport; properties of the phloem tissue. Phloem loading and unloading; hypotheses of phloem transport. Effects of damage of the bark on plants. Relevance of phloem transport in plant growth development and productivity. BOT 7228 Stress Physiology and Phytoremediation (2 CU) Course objectives At the end of the course, the student should be able to: Review the diversity of stress factors and their effects on plant metabolism Relate stress factors to plant production Classify plants according to their tolerance characteristics Identify bioindicators of environmental pollution/stress Identify suitable plant species for the restoration of degraded environments Water stress and aridity. Ecophysiology of C3, C4 and CAM plants. Metabolic, stomatal and growth responses to water stress. Water use efficiency. Drought avoidance and tolerance mechanism. Stress factors (radiation, temperature, salinity (alkalinity & acidity), oxygen, heavy metals) and their effects on primary productivity; anthropogenic factors; xerophytes, hygrophytes, metallophytes, toxicophytes and their adaptations to the stress factors, Bio-indicators of plant stress. Use of plants to restore degraded environments. BOT 7229 Plant Growth and Development (3 CU) Course objectives At the end of the course, the student should be able to: Describe the molecular basis of plant growth and development Describe the mechanisms of action and physiological roles of Phytohormones in plants Apply knowledge of Phytohormones in improving plant production Apply knowledge of Phytohormones for the preservation of plant products in the post-harvest period The molecular basis of plant growth and development. The role of plant growth hormones in regulating cellular activities and in correlative growth. The growth and development of the whole plant from seed germination to seed production. The influence of Phytohormones and environmental factors on plant growth and distribution. Mechanisms of hormonal action. Metabolism of plant growth hormones; Biosynthesis and catabolism. Interactions between Phytohormones. Detection, isolation, biological and chemical assay methods. Application of plant hormones in agriculture/horticulture as growth regulators; floral initiation; fruit growth and development; fruit harvesting and ripening; rooting from stem cuttings and uses as herbicides. Recombinant DNA techniques in Plant Physiology. Growth patterns of higher and lower plants. BOT 7230 Physiology of Growth and Crop Yield (2 CU) Course objectives At the end of the course, the student should be able to: Explain the effects of environmental on crop/plant yield Develop appropriate strategies spacing for optimal energy capture and production by plants Explain the reproductive and development patterns of different crops/plants and resource allocation Developmental and environmental physiology with particular reference to growth and yield of selected dicotyledonous plants and cereals. The development of leaf canopies and tiller production. Root growth. Reproductive growth and development. Resource allocation. Community physiology and growth. BOT 7231 Plant Energetics (3 CU) Course objectives At the end of the course, the student should be able to: Describe the leaf morphology and anatomy with respect to their photosynthetic potentials Explain the energy use efficiency of different plant types Outline the importance dark respiration and photorespiration on primary and secondary production in plants Explain the mechanisms of extrinsic and intrinsic regulations of photosynthesis Explain the variations in the growth rates of plants growing under different light regimes Morphology of leaf cells. Chloroplasts and their various activities. Photochemistry and photosynthetic electron transport. Biochemical pathways of CO2 fixation in photosynthesis. Dark respiration and photorespiration. Glycolate metabolism and the mechanism of photorespiration. Photosynthesis and physiological control of net photosynthesis in single leaves. Relations of photosynthesis, total respiration and other factors to control productivity in plant stands. Measurements of light in plant communities. BOT 7232 Plant Tissue Culture (3 CU) Course description Methods and techniques in plant cell and tissue culture; differentiation and organogenesis, application in agriculture, horticulture, forestry, medicinal plant conservation through clonal multiplication; laboratory exercises are included. Learning outcomes By the end of the course students should be able to: Establish and maintain organs using asceptic techniques in controlled conditions Interpret and understand basic information on induction of organs from callus of plant with applied influence of hormone and external factors Explain applications of plant tissue culture in molecular biology and genetic engineering to produce transgenic plants Course outline Introduction to plant tissue culture; general methods in plant tissue culture, laboratory and equipment; media components, preparation, auto claving and contamination; protoplast technology; regeneration and morphogenesis, cryopreservation theory and techniques; somatic embryology; physiology of plant cell culture; organogenesis; tissue culture of trees; tissue culture of medicinal plants and production of secondary metabolites in tissue culture; genetic engineering in plant tissue culture; application of plant tissue culture to industry. Appendix 1 Personnel FULL TIME STAFF 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. Dr. R. Bukenya-Ziraba Dip. Ed., B.Sc. (Mak.) M.Sc. (Ghana) Ph.D. (Mak). Professor. Dr. H. Oryem-Origa B.Sc., M.Sc., Ph.D. (Mak) Professor. Dr. G. Mutumba Dip. Ed., B.Sc. (Dar), Ph.D (Wales) Assoc. Prof. Dr. E. K. Kakudidi Dip. Ed., B.Sc. (Mak), Dip. Sc., M.Sc. (Aus. Nat. Univ.), Ph.D. (Mak) Assoc. Prof. Dr. P. Mucunguzi B.Sc. (Mak) M.Sc. (Mak), Ph.D (Mak) Assoc. Prof. Dr. S. Byarujali Dip. Ed. (Mak) M.Sc. (Mak) Ph.D (Mak), Lecturer Dr. S. Nyakaana Dip Ed., B.Sc., (Mak) M.Sc. (Wales) Ph.D,(Mak) Assoc. Prof. Dr. J. Kalema B.Sc., M.Sc., Ph.D (Mak) Senior Lecturer Mr. C. Nyakoojo, Dip Ed., B.Sc.,M.Sc. (Mak)., (Ph.D, student), Lecturer. Dr M. Kamatenesi Dip Ed., B.Sc., M.Sc., PhD (Mak), Senior Lecturer. Dr. P. Ipulet BSc, MSc, PhD (Mak), Lecturer. Mr. Tugume A. B.Sc., M.Sc.( Agric/) (Mak) (Ph.D student)Assistant Lecturer. Dr. Nabulo Grace B.Sc. (MU) M.Sc. M.Sc Assistant Lecturer. Ms. P. Tugume B.Sc. (MU), M.Sc. (MU) Teaching Assistant. Dr. P. Segawa BSc (Forestry), MSc., PhD (Mak), Herbarium Curator Dr. M. Namaganda BSc., MSc. (Mak), PhD (ULS), Principal Asst. Herbarium Curator Dr. E. Olet, BSc., MSc., (Mak), PhD (ULS) Lecturer PART TIME AND CONTRACT STAFF 18. 19. 20. 21. 22. 23. 24. 25. Dr. H. K. Taligoola B.Sc. (E.A.) M.Sc. Ph.D. (Nottingham) Professor – PartTime Mr. J. Kashambuzi, B.Sc. (M.U), M.Sc. (M.U) Senior Lecturer- Contract Dr. J.M. Kasenene Dip. Ed. (MU) B.Sc. M.Sc. (MU) Ph.D (Michigan) – Assoc. Professor- Contract Dr. J. Tabuti B.Sc. (MU), M.Sc.(MU) Ph.D. (AUN) Assoc. Prof. Mrs. B. S. Male-Kayiwa B.Sc. (MU) M.Sc. (Birmingham) – Part-time Lecturer. Dr. P.S.N Ssekimpi B.Sc. (MU), M.Sc. (MU) Ph.D. (Arizona) Part-time Lecturer. Dr J. Mpagi B.Sc (MU) M.Sc, PhD – Part time Lecturer J. Ssenteza BA., MA (Mak), Part-Time Lecturer APPENDIX II: FINANCES (a) FEES The fees for Master of Science (Botany) will initially be 1,800,000 per year or 540,000/= per semester. For non-Ugandans, the fees will be US $ 1000.00 per academic year, or $ 500.00 per semester. However, the University Council will review tuition and other fees from time to time. The earnings from the fees will be used to cover aspects like field trips for practical training, inviting and paying guest Lecturers, buying equipment etc. Savings from this money will also be used for capital development like creating space on other teaching facilities like purchase of computers, chemicals, etc. (b)- Student Capacity The program shall initially admit up to 25 students as private students per year. (c) ESTIMATED REVENUE FROM SELF SPONSORED STUDENTS (i) Estimated number of students 25 at 1,800,000= (ii) Summary of expenditure Day Programme (25 students) 1. 2. 17,550,000/= Balance (61%) 27,450,000/= Faculty (20%) Department (80%) 5,490,000/= 21,960,000/= Details of expenditure - Day program (i) Teaching Expenses: Teaching for 1200 hrs on average per year at 15,000/= 18,000,000/= Personnel Allowances Program co-ordination honorarium per annum (iii) 45,000,000/= Central activities (39%) (d) (ii) 45,000,000/= Stationery 360,000/= Photocopying 10,000 x 10 reams 100,000/= Printer toner (1 x 250,000/=) 250,000/= Photocopier toner (1 x 250,000/=) 250,000/= Sub-Total 600,000/= (vi) Laboratory and field Work (Government + Private) 3,000,000/= Grand Total Ug. Shs. = 21,960,000/= MAKERERE UNIVERSITY FACULTY OF SCIENCE DEPARTMENT OF CHEMISTRY A CURRICULUM REVIEW OF MASTER OF SCIENCE IN CHEMISTRY PROGRAMME MARCH 2006 THE DEGREE OF MASTER OF SCIENCE IN CHEMISTRY BY COURSE WORK AND DISSERTATION I : BACK GROUND The Department of Chemistry opened in 1944 and was at its best both in research and teaching from 1970 to 1974. At the end of that period most of the academic staff abandoned University service because of the prevailing political and economic turmoil. This period was followed by a general decline in research, teaching and qualified personnel capacity. Since 1980, the department has been recovering slowly in all these aspects although the ability to develop staff has remained a problem. Secondly, although chemistry has been taught at Makerere University for the last 50 years the impact of the subject on society is not obvious. This could be attributed to the inherent inability of the undergraduate program to relate to local issues of scientific nature and hence stimulate thinking and action on local problems. Currently, the department has two professors, one associate professor, five senior lecturers, seven lecturers, two assistant lecturers and five teaching assistants. In addition to these, Uganda now has a pool of qualified PhD holders outside the university form which the department can draw part-time lecturers in critical shortages of staff. In terms of facilities and space, the department has nine (9) research laboratories capable of housing 40 research students in addition to six undergraduate laboratories. The department has a GC-MS, two gas liquid chromatographs, a polarographic analyzer, an atomic absorption spectrophotometer, HPLC, IR, UV, a fully equipped unit operations laboratory which can be utilized for industry-related research and other small equipments. II: JUSTIFICATION In spite of having a good number of qualified academic staff, numerous research laboratories and an annual average intake of 80 students offering chemistry in their first year, chemistry as a science has not made a noticeable impact on society in general and on industry in particular. The department has an obligation to provide research scientists for both industry and research institutions. These institutions have so far found our graduates wanting in many aspects especially in applying their training to real life practical situations. The course outlined below is designed to answer the needs of industry, research institutions and colleges. It provides a short-cut to long laborious hours of unfruitful research which have been a deterrent to postgraduate work in chemistry. For this reason the course should be attractive to employers who need more knowledgeable, quickly and cheaply trained chemists for their work. The course being proposed is intended for the following categories of people: (a) (b) ( c) Graduate students who wish to work in research institutions and industries. Career research scientists working in research institutions. Career teachers who wish to broaden their background in chemistry. III: OBJECTIVES The main objectives of the course being proposed will be two-fold: (a) (b) To provide advanced knowledge in chemistry and applied chemistry to first degree holders. To develop researchers in chemistry and applied chemistry and nurture a quality assurance-conscious group of scientists in order to safeguard users of chemicals. In the process of fulfilling these objectives, it is our hope that: (1) the participants will develop critical and analytical attitudes and skills towards problem finding and solving. (2) the participants will have broader views on environmental problems and develop research oriented attitudes to environmental conservation. IV: REGULATIONS The regulations for the degree of Master of Science in Chemistry including the curriculum and syllabuses the degree are attached. V: RESOURCES The Department of chemistry is an established department within the Faculty of Science. In addition to the physical facilities, the Department has qualified personnel as well as the basic laboratory equipment to facilitate the running of the course being proposed. REGULATION FOR THE DEGREE OF MASTER OF SCIENCE (MSC) IN CHEMISTRY BY COURSE WORK AND DISSERTATION 1. GENERAL Admission to the Masters Degree in Chemistry will be governed by the general Makerere University and Faculty of Science entrance requirements for postgraduate work. In particular the following regulations will apply: 2. ADMISSION Candidates should be in possession of at least an upper second class honors degree in chemistry of chemistry combined with another subject from a recognized University. Candidates with lower qualifications will be considered for admission provided they have demonstrated academic growth in chemistry or in one of the specified options. 3. CURRICULUM i) The duration of the program will be two calendar years or four semesters. Each Semester shall consist of twenty weeks of teaching and three weeks of examinations. The program will consist of two parts; part I (Course Work) and part II (Research and Dissertation). Part I of the program will be completed in two semesters and will consists of course work with sixteen course units or a total of eight courses. One courses unit shall be equivalent to one contact hour per week per semester. One hour of lecturers shall be equivalent to one contact hour, Two hours of ii) iii) iv) v) vi) viii. ix) practical shall be equivalent to one contact hour and one tutorial hour shall be equivalent to one contact hour. There will be four compulsory courses which shall be completed in the first semester. The four courses CHM 7102, CHM 7102, CHM 7103 and CHM 7104 have been designed to give candidates a broad background knowledge on relevant topical issues in chemistry. Four courses shall be completed in the second semester. At least three of the courses shall be selected from any of the given areas in consultation with the supervisor(s). In part II of the program, candidates will undertake individual research projects in their areas of specialization which will form the subject of a concise dissertation. The areas of specialization are: Inorganic Chemistry Analytical/Environmental Chemistry Organic Chemistry Physical Chemistry Applied Chemistry COURSE WORK (PART I) First Semester COMPULSORY COURSES Every candidate will be required to do the following courses in the first semester: Code Course title Lecture hours Contact hours Course Units CHM 7101 Research methodology 40 40 2 CHM 7102 Instrumentation 40 40 2 CHM 7103 Key aspects of 40 Uganda’s Environment Atmospheric Chemistry 40 40 2 40 2 CHM 7104 b) Second Semester In the second semester, every candidate will be required to take four courses from the list given under the five specialized areas of research below: At least three courses must be drawn from one area of specialization and where necessary the fourth course will be a related course selected from other areas. The table below gives the areas of specialization and the courses in each area. I. INORGANIC CHEMISTRY Code Course title CHM 7205 Advanced chemistry of p- 40 block elements Chemistry of metal clusters 40 CHM 7206 CHM 7207 CHM 7208 II. Lecture hours Coordination compounds 40 & Redox reactions Organometallic & 40 bioinorganic chemistry Contact hours 40 Course units 40 2 40 2 40 2 2 ANALYTICAL/ENVIRONMENTAL CHEMISTRY Code CHM 7209 CHM 7210 CHM 7211 CHM 7212 Course Title Analytical Methods Contact hours 40 Separation and analysis of 40 real samples Chemistry of water 40 environment Land pollution 40 Lecture hours 40 Course units 2 40 2 40 2 40 2 III ORGANIC CHEMISTRY Code Course title Advanced spectroscopy Contact hours 40 Lecture hours 40 Course Units 2 CHM 7213 CHM 7214 Organic synthesis 40 40 2 CHM 7215 Natural Products 40 chemistry Synthesis of heterocyclic 40 natural compounds 40 2 40 2 CHM 7216 IV PHYSICAL CHEMISTRY Code CHM 7217 CHM 7218 CHM 7219 Course title Advanced thermodynamics Advanced reaction kinetics Advanced topics in electro chemistry Lecture hours 40 Contact hours 40 Course units 2 40 40 2 40 40 2 V APPLIED CHEMISTRY Code CHM 7220 CHM 7221 Course Title Ceramic, Glass & Cement industries Food & Fermentation industries Contact hours 40 Lecture hours 40 Course units 2 40 40 2 CHM 7222 CHM 7223 CHM 7224 Sodium & Phosphorus 40 industries Geochemical 40 techniques Water-Interactions 40 40 2 40 2 40 2 4. EXAMINATION REGULATIONS 4.1 There will be written examinations at the end of each Semester of part I of the program. Written examinations shall constitute 70% of the marks, progressive assessment shall contribute the remaining 30%. Progressive assessment shall consist of tests, assignments and/or seminar presentations. At least 2 assignments/tests shall be set per course per semester. For each course the assignments/tests will be weighted at 1.2 course units. 4.2 4.3 4.4 Pass Candidates will be deemed to have passed part I of the program under the following conditions: a) have obtained at least 60% in each course. b) Have passed at least two papers in each semester and have obtained an average of 60% with not less than 55% in any one paper at a first sitting. c) Have obtained 60% in each of failed papers at a supplementary sitting. d) Have obtained 60% in each of the failed courses on repeating. 4.5 Supplementary examinations Supplementary examinations will be held within a period of six weeks after the end of the second Semester of part I of the program. A candidate who fails to pass part I will be allowed to sit for a supplementary examination of the courses failed provided he/she has not been repeating part I. 4.6 Repetition Repeating of Part I will only be allowed on failure to pass the supplementary examination for candidates who are not already repeating. 4.7 Discontinuation a) A candidate who fails to qualify for supplementary examination after failing part I of the program shall be discontinued. b) A candidate who fails part I of the program when repeating shall be discontinued. c) A candidate who fails a re-submission of the dissertation shall be discontinued. 5. Scheme of Course work examination a) First Semester Paper Course(s) Examination Marks Paper 1 CHM 6201 3 100 Weighting hours 0.15 Paper 2 CHM 6202 3 100 0.15 Paper 3 CHM 6203 3 100 0.15 Paper 4 CHM 6204 3 100 0.15 Paper 5 Progressive Assessment - 100 0.40 b) Second Semester At the end of the second semester, each candidate will sit four papers, three of which must be from his area of intended specialization. Each paper will be of a three hour duration and will be marked out of 100%. 6. RESEARCH AND DISSERTATION (PART II) a) A candidate will be required to pass part I before proceeding to part II of the program. b) A candidate will be required to carry out research in one of the option areas. c) Each candidate will be required to submit a project proposal approved by the Departmental Research Committee to the Faculty Research and Postgraduate Committee within the second semester of the first part of his program. d) A dissertation shall be submitted at the end of part II. e) The university regulations governing the submission and examination of these will apply to this part of the program. 7. AWARD OF THE DEGREE The degree of Master of Science in Chemistry shall be awarded to a candidate who successfully completes all parts of the program. The degree of Master of Science in Chemistry shall be awarded without classification; however, the Academic transcript shall be graded as follows: A = B+ = B = C = D = E = 8.0 80 – 100% 70 – 79% 60 – 69% 50 – 59% 45 – 49% (Compensatable Fail) 0 – 44% (Fail) OUTLINE OF THE COURSES CHM 7101 RESEARCH METHODOLOGY 2 CU The planning or research projects. Experimental design and the scientific method. Collection, organization, analysis and presentation of data in tabular and graphical forms. Types of scientific calculators and computers. Introduction to programming. Microcomputers and their applications. Information handling, storage and retrieval. Types of statistical analyses and their applications. Interpretation of results and preparation of scientific reports. CHM 7102 2 CU INSTRUMENTATION This course will cover the theory behind the principal types of chemical instrumentation. The spectroscopic techniques will include ultra-violet and visible spectrophotometry, atomic absorption spectrophotometry, infrared spectrophotometry, nuclear magnetic resonance spectroscopy and electron spin resonance spectroscopy. Chromatographic techniques will include gas chromatography and high performance liquid chromatographic methods. Electrochemical techniques will include polarographic methods. Miscellaneous techniques such as mass spectrometry, and gas chromatography-mass spectrometry will also be discussed. CHM 7103 KEY ASPECTS OF UGANDA’S ENVRIRONMENT 2 CU Major physical resources: water, rocks and minerals; their distribution and exploitation. The human population; its composition, distribution in relation to climate, history and trends. National and international data bases. An outline of environmental laws and agreements; national and international. CHM 7104 ATMOSPHERIC CHEMISTRY 2 CU Physical characteristics of the atmosphere and its stratification. Ions and radicals in the atmosphere. Reactions of atmospheric oxygen, nitrogen and carbon dioxide. Sources, composition and environmental effects of particles. Gaseous inorganic pollutants, e.g. oxides of carbon, nitrogen, sulphur and halogen compounds; their reactions and effects on the environment. Organic air pollutants; their sources and effects on the environment. Smog-forming automotive emissions; reactions and mechanism of formation. I. INORGANIC CHEMISTRY CHM 7205 ADVANCED CHEMISTRY OF p-BLOCK ELEMENTS 2 CU Advanced treatment of bonding and structure of boranes and their derivatives. Widespread manifestation of concept of disproportionation among compounds of these elements. Redox interactions in thiosulfate and polythionate systems and mechanisms thereof. Advanced treatment of halogens and their derivatives: polyhalgen and interhalogen cationic systems; polyhalile anionic systems, both mono and mixed; the treatment to include preparation of these systems, their structures and stability. CHM 7206 CHEMISTRY OF METAL ATOM CLUSTERS 2 CU Distinguishing criterion of metal atom cluster compounds and extent of nuclearity. Bonding and structure in metal cluster: metal-metal multiple bonding; cluster valence electron (CVE) counting scheme and its correlation with structure; isoelectornic and isolabal relationship in clusters. Varieties of metal clusters: cabonyl clusters and their derivatives containing hydride, carbide, nitride, phosphide, and sulphide species; survey of clusters for various transition elements. Synthesis of the clusters and their reactions. CHM 7207 COORDINATION COMPOUNDS AND REDOX REACTIONS 2 CU This course will cover introduction to ligand substitution reactions in octahedral, square planar, and complexes of other geometries. Racemization reactions, catalysis of substitution by redox processes and photochemical reactions will be discussed. Conventions regarding standard states, methods of determining changes in free energy, calorimetric data, schematic representation of galvanic cells, Pourbaix diagram as well as periodic trends among the transition elements and their half-cell emf values will also be covered in this course. CHM 7208 ORGANOMETALLIC AND BIOINORGANIC CHEMISTRY 2 CU The course will cover carbonyl complexes, bonding or organic ligands to metals, experimental evidence of back donation, survey of -donor complexes, complexes with metal-carbon sigma bonds, selected stiochiometric reactions of organometallic and homogenous catalysis by soluble transition metal complexes. Selected aspects of bioinorganic chemistry including essential elements, blood and vitamin B12, means of supplying iron in lower organisms, the role of copper as well as photosynthesis will also be discussed. II ANALYTICAL/ENVIRONMENTAL CHEMISTRY CHM 7209 ANAYTICAL METHODS 2 CU Evaluation of analytical data including detection and correction of determinate and indeterminate errors as well as discussion of significance tests. Reagents and calculations used in titrimetric methods of analysis such as precipitation, complexometric and amperometric titrations will be discussed. CHM 7210 SEPARATION AND ANALYSIS OF REAL SAMPLES 2 CU Analytical separations and the nature of separation processes. A discussion of separation processes will cover precipitation, extraction, ion exchange and distillation. Chromatographic separation methods will include column chromatography, paper chromatography, ion exchange chromatography as well as gas liquid chromatography. The choice of method for analysis of complex samples and their preliminary treatment will be illustrated through discussion of case studies.. CHM 7211 CHEMISTRY OF WATER ENVIRONMENT 2 CU Fundamentals of aquatic chemistry; water acidity and alkalinity. Metal species and the nature of their existence. Aquatic biochemical processes; microbial transformations of carbon, nitrogen, sulphur, metals and metalloids and their compounds. Properties of water and water bodies. Types and courses of water pollutants. Methods of water treatment for various uses. Industrial wastes and disposal with respect to Uganda. CHM 7212 LAND POLLUTION 2 CU The nature of solids in the geosphere. Sediments, clays and soil. Soil chemistry including acid base reactions and micronutrients. Agricultural land pollutants; fertilizers, pesticides and herbicides. Sources and nature of hazardous wastes; their chemistry and methods of disposal. III . ORGANIC CHEMISTRY CHM 7213 ADVANCED SPECTROSCOPY 2 CU A review of structure identification by use of the following methods will be made: Infrared spectroscopy, ultraviolet spectroscopy, nuclear magnetic resonance spectroscopy and mass spectrometry. Carbon-13 NMR will be discussed by interpretation of spectra for specific examples of compounds. CHM 7214 ORGANIC SYNTHESIS 2 CU Synthetic methodologies introduced at undergraduate level will be reviewed. Synthetic methods will be illustrated with examples from natural products such as Dictamine, Flindersine, Penecillins and Cephalosporines. The methodology of planning an organic synthesis will be discussed through examples of natural products and other molecules of theoretical interest. The aim is to introduce a student to formulating possible sequences for target molecules and critically appraise each sequence from the point of view of probability of success and efficiency of the yield. Synthetic targets for study will be chosen in such away as to highlight general synthetic reactions and modern techniques of structure identification. CHM 7215 NATURAL PRODUCTS CHEMISTRY 2 CU The aim of this course is to show how organic constituents of living organisms are produced in nature. It will cover the distinction between primary and secondary metabolites, fatty acid biosynthesis as an example of primary metabolism, the acetyl coenzyme A and the acetate-malonate pathways, theories of biogenetic pathways of natural products in living organisms. The biosynthesis of terpeniods, steroids and alkaloids will be discussed with the help of specific examples. CHM 7216 SYNTHESIS OF HETEROCYCLIC NATURAL COMPOUNDS 2 CU This course will review the structure, reactivity and synthesis of a representative number of heterocycles. In addition, the chemistry of heterocycles of pharmaceutical interest such as penicillins, antibacterial agents, anticonvulsive agents, antipyretic agents, barbiturates, stimulants, vitamins B1, B2, B12 etc will be discussed. The use of simple heterocyles in the synthesis of complex natural products will be illustrated with emphasis on heterocycles as strategic synthons. Examples to be discussed will include synthesis in which heterocycles are performed and those in which ring formation is a key step. Use of heterocycles for activation and masking of functionality will also be discussed. IV PHYSICAL CHEMISTRY CHM 7217 ADVANCED THERMODYNAMICS 2 CU The laws of thermodynamics and equilibrium will be reviewed. Activity coefficients will be stressed. Depending on the area of interest of the student, one or more of the following will be treated in detail: thermodynamics and electrode potentials; thermodynamics of the activated state in solution; bond energies and thermodynamic measurements; phase diagrams, especially as applicable to the ceramic, cement and metallurgical industries; thermodynamics of biochemical compounds; thermodynamics of industrial chemical systems. CHM 7218 ADVANCED REACTION KINETICS 2 CU The mathematics of rate equations. Computer models for rate equations. Experimental methods, including relaxation methods. Catalysis. Kinetic studies of short-lived species by mass spectrometry, and matrix isolation. Detailed treatment of the theory of reaction rates; the collision theory and the transition state theory. Mechanisms and the steady state approximation. CHM 7219 ADVANCED TOPICS IN ELECTROCHEMISTRY 2 CU This course will cover irreversible electrode processes, electrode kinetics, corrosion science, potentiometry and ion selective electrodes. V. APPLIED CHEMISTRY CHM 7220 CERAMIC, GLASS AND CEMENT INDUSTRIE 2 CU The chemistry and manufacture of ceramics, white wares, structural clay products, refractories, enamels and enameled metal, portland, pozzolan and magnesium oxchloride cements. The composition, chemistry and manufacture of glass. CHM 7221 FOOD AND FERMENTATION INDUSTRIES 2 CU Conventional and nonconventional foods. Food, milk, meat, poultry and by-product processing. Mechanical and chemical preservation of foods, chemistry and mode of action for food additives. Manufacture of absolute and industrial alcohols, wines and liquors, vinegar and acetic acid, lactic acid and enzymes. CHM 7222 SODIUM AND PHOSPHORUS BASED INDUSTRIES 2 CU The uses and manufacture of various salts of sodium such as sodium chloride (common salt), sodium sulphite, sodium sulphate, sodium silicates, etc. The phosphate rock and the manufacture of super phosphates and fire retardant phosphorus based chemicals. CHM 7223 GEOCHEMICAL TECHNIQUES 2 CU Chemistry of Minerals and Rocks. Techniques of Rock collection and Mineral separation. Rock dissolution and wet chemical analysis including AAS, ICP, MassSpectrometry, Dry techniques, Rock Thin Section, Staining, Catholominescenece, XRD, XRF, Microprobe, SEM, TEA, DTA Neutron Activation. In each case the aspects relating specifically to Rock Analysis will be explored rather than general discussion of techniques. CHM 7224 WATER-ROCK INTERACTIONS 2 CU Chemistry of continental and oceanic water. Weathering processes. Sedimentation processes, Isotope Geochemistry D, 18O. Rate of water in magmatic process, mineral deposition and metamorphic reactions. Pollution, aspects relating to Environmental Geochemistry. MASTER OF SCIENCE IN MATHEMATICS Semester I Core Courses Advanced Group Theory Functional Analysis Measure theory & Probability Further Modules and Rings Semester II Core Courses Topology Stochastic Differential Equations Dynamical Systems and chaos Partial Differential equations Semester III Research methods and proposal writing Two advanced course taken among the list of electives that are related to the students’ project area. Elective Courses (Choose 2) Field Theory Commutative Algebra Homological algebra Harmonic Analysis Measure and Integration Topology and Lie groups Geometry of Manifolds Topics in Complex Analysis Mathematical Epidemiology Mathematical Ecology and Natural Resource Modelling Operations Research and Optimization Theory Financial Mathematics Life Insurance Mathematics Non-Life Insurance Mathematics Biostatistics and Biodata Modelling Statistical Modelling Mathematical Economics/Game Theory Control Theory Hydrodynamic Stability Theory Environmental Modelling Time Series and Forecasting Semester IV Dissertation CURRICULUM: MASTER OF SCIENCE PHYSICS Course work will last two semesters and the following courses will be taken. (a) Course arrangement (i) Semester I Core courses: PHY 7108 PHY 7101 PHY 7102 PHY 7109 One elective Computer Science Methods of Mathematical Physics Classical Mechanics Research Methods (ii) Semester II 3 CU 3 CU 3 CU 2 CU 3CU Core courses: PHY 7206 PHY 7205 PHY 7207 One elective (b) Statistical Mechanics Classical Electrodynamics I Advanced Quantum Mechanics I Dissertation 3CU 3CU 3CU 3CU 26 CU Research towards a dissertation will last two semesters. (a) CURRICULUM: DOCTOR OF PHILOSOPHY PHYSICS Course arrangement The course work will last one semester and the following courses will be offered. (i) Semester I PHY 9105: Classical Electrodynamics II PHY 9107: Advanced Quantum Mechanics One elective from the 700 series RESEARCH (ii) Semester II 3CU 3CU 3CU (b) Research towards a dissertation will last through Semesters II – VI. A candidate will work with one of the research groups in the Department. EXAMINATION Every course unit will be assessed out of 100 marks allocated as follows: Progressive assessment Written Examination The pass mark will be 40% 60% 60% The M.Sc. dissertation will be graded out of 100 marks. Each course unit will be graded out of a maximum of 100 marks according to the following scale. Marks Grade Points 80 - 100 75 – 79.9 70 – 74.9 65 – 69.9 60 – 64.9 55 – 59.9 50 – 54.9 45 – 49.9 Below 45 A B+ B BC+ C CD E 5 4.5 4.0 3.5 3.0 2.5 2.0 1.5 0.0 To obtain an M.Sc. in Physics a candidate must have accumulated at least 26 CU in the courses and submitted a dissertation equivalent to 26 CU To obtain a Ph.D. in Physics a candidate must have accumulated at least 9 CU in the courses and submitted a dissertation. COURSES CODE PHY 7101 PHY 7102 PHY 7106 PHY 7108 PHY 7109 PHY 7205 PHY 7207 PHY 9105 COURSE NAME Methods of Mathematical Physics Classical Mechanics Statistical Mechanics Computer Science Research Methods Classical Electrodynamics I Advanced Quantum Mechanics I Classical Electrodynamics II HOURS 45 45 45 45 30 45 45 45 CU 3 3 3 3 2 3 3 3 PHY 9107 Advanced Quantum Mechanics II 45 3 ELECTIVE COURSES CODE COURSE NAME PHY 7103 Solid State Physics PHY 7104 Energy Physics PHY 7202 Geophysics PHY 7203 Materials Science PHY 7204 Radiation Physics PHY 7105 Atmospheric Physics PHY 7206 Astrophysics HOURS 45 45 45 45 45 45 45 CU 3 3 3 3 3 3 3