Name:____________________________ Lab Partners:____________________________________________________ Mrs. Gomez Biology Due Date:_________________________ Osmosis Potato Lab Introduction: Purpose: The purpose of this lab is to determine what percent of sugar each type of osmotic solution will occur. Potato pieces will be placed in fresh water, and three types of sugar solutions, the direction of water movement will be determined based on the sugar concentration of the potato. Background: Answer the following questions within the body of your lab report. 1. What is diffusion? Give an example. 2. What is osmosis? 3. Describe the three types of osmotic solutions. 4. Give at least one example of a way you can create a hypertonic solution and one way you can create a hypotonic solution within your body. 5. Explain why water moves (equilibrium) **Be sure to make proper citations from the textbook or other source (Biggs et al, Pg #) Hypothesis: Complete the following statements: The freshwater will be a _______________solution because....... The 2% sugar solution will be a ________________solution because.. The 4% sugar solution will be a ________________solution because.. The 6% sugar solution will be a ________________solution because.. Design Setup: Attach a hand drawn diagram to the lab, you may also use some clip art All materials used Label materials Label all solution types Provide a purpose or justification for each material Materials: 1. Beakers or plastic cups, 4 2. fresh water, 40 mL 3. 2% sugar water, 40 mL 4. 4% sugar water, 40 mL 5. 6% sugar water, 40 mL 6. Potato pieces, 4 pieces 7. triple beam balance 8. paper towels 9. forceps 10.marker Procedure: 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. Collect all materials Pat pieces of potato dry with paper towel Place each piece of potato is a different beaker and label the beakers Remove piece of potato and weigh using the triple beam balance Record each weight in grams in your table In beaker A add 40mL of freshwater from flask In beaker B add 40mL of 2% sugar solution In beaker C add 40mL of 4% sugar solution In beaker D add 40mL of 6% sugar solution Label the beakers with pod names and place in safe place, allow to sit for 48 hours After 48 hours, remove potato from beaker and pat dry with paper towel Weigh each potato with triple beam balance and record in table Discard potato pieces, paper towel and solutions Determine the change in mass (final mass - initial mass) Determine the % change in mass (change in mass/initial mass) Determine if the solution is isotonic, hypertonic or hypotonic based on the movement of water Graph the % change as a line graph (x-axis - solution, y-axis - % change) Data: Potato A Potato B Potato C Initial Mass (g) Final Mass (g) Change in Mass (g) % Change in Mass Movement of water Type of solution make sure you explain what data you are showing in the table Potato D Graph your data and make sure you explain the graphs Calculate the isotonic solution Analysis: Explain what happen in the lab, which potato gained/lost mass and how you justified the type of solution Conclusion: 1. Restate purpose statement 2. Restate your hypothesis 3. Accept, reject or partially accept your hypothesis and explain (This is where you need to bring in supporting data) 4. Explain what type of solution was in each beaker 5. Use the solution terms and terms that you have used within the introduction 6. Estimate the % sugar in the potato based on your data (predict the isotonic solution) Possible Errors and Critical Thinking: What are some possible errors (human, experiment?) Why is it important to understand osmosis What is a question that you came up with during the experiment that could have affected your data What did you learn from this lab Works Cited: create a bibliography using MLA format Textbook: Biggs, Alton, and Whitney C, Hagins. Biology: The Dynamics of Life. New York: McGraw Hill Companies, Inc. 2004. Make sure to include any other sources that you used. Helpful Hints: Double Space Keep data tables and graphs on one page underline heading and space after sections set margins at 1 inch NO PERSONAL PRONOUNS Have someone proof read your report Make sure everything is labeled properly (graphs, tables, design setup, etc.) Do not forget to turn in sections of your lab report (Design setup, graphs, etc.) Read and follow your lab report rubric, you need to include more detail than is included here in order to get a 5 Do not forget units anywhere Look over your final product and check the formatting