The production of a priority list of crop wild relatives for Ireland The aims of this project were:1. Critical overview of Markkola (2005) who prepared a previous list of Crop Wild Relatives based upon IUCN Threat categories. 2.The establishment of criteria for Priority Listing including reference to Annex I of ITPGRFA, food potential, actual and potential economic benefits, socio-economic issues and population viability for exploitation. 3.Application of IUCN status to those species qualifying which do not occur on the Markkola’s list. 4.Production of a Priority List of Crop Wild Relatives for Ireland based on Criteria 2 above. 5.Pilot field study in SE Irelands on populations of a species (Black mustard) with a view to establishing a protocol for survey of other such population data-deficient species. 6.The collection of seed of black mustard for genebank storage and genetic investigation by UCC of selected populations from SE Ireland to establish its genetic characteristics. 7.Production of final report. Methods: Two publications Census catalogue of the flora of Ireland (Scannell & Synnott, 1987) and A catalogue of alien plants in Ireland (Reynolds, 2002) were used to provide an inventory of the entire flora of Ireland numbering c 2,000 species. The first step in establishing the CWR list was to screen the entire flora for the presence of those taxa listed on Annex I of the ITPGRFA. The second step was to screen the entire list of the Irish flora for those species which are not on Annex I but are known to have actual or potential for food, agriculture or forestry from standard texts and websites. The result of this was to arrive at a list of 181 CWRs for Ireland, each according to a set of 20 criteria which are listed on an EXCEL spreadsheet and Access database,. Summary accounts of the uses and value of each and whether there are known cultivars of Irish origin derived from them are also presented. Each species was then ranked and scored on a scale of 0-12 against four criteria and Priority was accorded to those which scored 8 or greater. Results: Following the compilation of a list of 181 species of Crop Wild Relatives for Ireland, 31 are considered to warrant Priority-listing for Ireland. Twenty one of the species are wild, five species are alien though all are well-established in the wild and five are cultivars which, occur as relics of or escapes from cultivation. Sixteen species on the current Priority list are of value and importance as Forage crops, three of which are Clovers and the remainder are grasses. The rest of the list contains five cereals whilst ten are of importance as Food crops, nine as herbs/vegetables and one fruit. A priority now should be the collection of seed material for all these species for storage in the national seed and gene bank with duplicates being lodged abroad. Comprehensive collections of some of the Legume and Grass Forages are being maintained at Oakpark but some species-Meadow and Red fescues are poorly represented and some not at all-Sheep’s fescue. Those species should be accorded priority for collection. The cereals on the list are reasonably represented in seed/gene banks with the exception of Rye but recent collections for the Landrace project have increased the number of accessions. Some collections of the herbs/vegetables notably Wild turnip and the Water-cresses are minimally or not at all represented, including Wild carrot. There is also a pressing need to increase the number of accessions of the four Protected and RDB-listed species. The current collections are inadequate to ensure the maintenance of viable, genetically-diverse stock. A practical approach to the collection of the Priority species would be to maximise efforts by concentrating of sites, such as species-rich forage areas and on sites on which a number of Priority CWRs occur together. The next stage should aim for the collection of a comprehensive range of accessions for all 31 Priority CWR species. The Pilot study on Black Mustard (Brassica nigra) demonstrated some practical difficulties in that some sites previously described for the species were found to be in error and only four authentic accessions were collected. The very wet summer also made the collection of dry material for genetic analysis difficult and it was necessary to have seed germinated in the laboratory to provide samples. Project Coordinator: Dr Tom Curtis