Supplementary table 1. Taxonomic assignment of significant populations predicted by
AdaptML.
Population
Closest relative
Method1
1
2
3
4
5
6
7
8
9
10
11
12
13
14
Vibrio aestuarianus
Vibrio ordalii
Enterovibrio calviensis
Enterovibrio norvegicus
Vibrio breoganii
Vibrio crassostreae
Vibrio sp. F10
Vibrio sp. F12
Vibrio splendidus
Vibrio kanaloae
Vibrio tasmaniensis
Vibrio tasmaniensis
Vibrio gigantis
Vibrio cyclotrophicus
16S (99.6%)
MLSA
MLSA
MLSA
MLSA
MLSA
MLSA
MLSA
MLSA
16S (99.7%)
MLSA
MLSA
16S (98.9%)
MLSA
Population ID
in 20062
G3
G1
G2
G9
G13
G10
G12
G18
G16
G16
G15 (II.A)
1
In case of populations congruent with a significant population identified in our first sampling in
2006, taxonomic assignment is based on multilocus sequence analysis (MLSA) as described in
(Preheim et al, 2011b). Closest relative of populations, which were not clustered with a significant
population from 2006, are given on the basis of partial 16S rRNA gene sequence similarity.
2
Group numbers of significant populations in 2006 as described in (Hunt et al, 2008a).
Supplementary table 2. Comparison of nutrient concentrations at the three sampling dates.
9/06/061
10/02/092
10/06/092
Temperature
(°C)
16
13.5
13.5
NH4+
(µg N/L)
144
26
17
Silicate
(µg Si/L)
ND
122
136
PO43(µg P/L)
25
2
1
ND – not determined
BDL – below detection limit
1
analyses performed at the University of New Hampshire, Durham, NH
2
analyses performed at Woods Hole Oceanographic Institution
NO3-/NO2(µg N/L)
5
BDL
3