Name: ______________________________________ Unit 6: Asexual & Sexual Reproduction Part A: Cells cell theory cell division Per. ________ Part E: Meiosis Part B: Asexual Reproduction Asexual Reproduction o Binary fission ex. o Budding ex. o Spore Formation ex. o Regeneration ex. o Vegetative Propagation ex. Sexual Reproduction o Sex cells (gametes): egg & sperm o Fertilization zygote o Homologous Chromosomes haploid/ diploid/ monoploid/ n/ 2n Meiosis = reduction division o Significance of meiosis o synapsis / tetrad formation o gametogenesis o spermatogenesis o oogenesis polar bodies Part C: Chromosomes chromatin chromosome histone chromatid centromere autosomes Karyotype diploid haploid Part D: Mitosis cell division Interphase Phase of mitosis o Prophase chromosomes centrioles/asters spindle fibers o Metaphase equator traction fibers o Anaphase o Telophase cleavage furrow cell plate daughter cells Cytokinesis Differences between plant and animal mitosis cancer 1 Part A: Cells Cell Theory All living things are made of cells Cells are the basic unit of life All cells arise from pre-exisiting cells Cell Division Cells produce new cells through the process of cell division Cell division different in prokaryotes and eukaryotes Some tissues are repaired more often than others o ex: WBC’s (white blood cells); skin cells; gut; embryonic cells o cells that don’t divide after birth: RBC’s (red blood cells); nerve cells; brain cells Reason for Cell Division Cell growth Repair & replacement of damaged cells Reproduction of species Types of Reproduction Asexual reproduction Sexual reproduction Part B: Asexual Reproduction type of reproduction that involves 1 parent offspring is genetically IDENTICAL to the parent more common in invertebrate animals than in vertebrate animals unicellular & multicellular plants can reproduce both asexually & sexually Cloning - offspring from a single body cell of a parent Types of Asexual Reproduction: 1. binary fission -simplest type of asexual reproduction -cell splits in half -daughter cells equal in size -ex. amoeba, algae, bacteria, protozoa 2. budding = growth of small reproductive fragment from parent. -unequal cell division ex. yeast, hydra 3. sporulation -multiple asexual reproductive cells released from parent ex. ferns, mosses, fungi 2 4. regeneration -new organism develop from part of the parent -replacement of lost body parts ex. planaria, sponges, starfish, lobster claw 5. vegetative propagation -asexual reproduction from parts of plant o runners: strawberries o tubers: potatoes o bulbs: onions o rhizomes: iris o cutting: aloe o grafting: similar plants Label the following diagrams of types of asexual reproduction as binary fission, budding, sporulation, regeneration, or vegetative propagation. Give two examples of organisms that use each method of reproduction. 1. 2. 3. Type: binary fission Type: sporulation Type: budding Examples: amoeba Examples: fungi Examples: yeast bacteria mosses 4. hydra 5. Type: vegatative propagation Type: regeneration Examples: strawberries Examples: starfish Potatoes planaria 3 Practice Questions ______ 1. Which method of reproduction is shown in the diagram at the right? 1. stem cutting 3. tuber formation 2. budding 4. sporulation ___1__ 2. Which diagram represents binary fission? sporulation 3. The diagram at the right includes information on asexual reproduction. Which term belongs in the area labeled X? Types of Asexual Reproduction Binary Fission Budding Amoeba Hydra X Bread Mold Vegetative Propagation Potato ______ 4. Which phrase best describes the process represented in the diagram below? 1. germination of a pollen grain in a flower 2. identical gametes being formed by mitotic cell division 3. development f seeds in an ovule 4. daughter cells being formed by mitotic cell division ______ 5. In certain plants, roots will develop from a stem cutting. This type of reproduction is known as 1. vegetative propagation 2. binary fission 3. unicellular budding 3. spore formation ______ 6. One way to produce large numbers of genetically identical offspring is by 1. cloning 3. changing genes by agents such as radiation or chemicals 2. fertilization 4. inserting a DNA segment into a different DNA molecule ______ 7. Potatoes, strawberries, and seedless oranges are generally produced by an asexual means of reproduction known as 1. sporulation 2. budding 3. binary fission 4. vegetative propagation 4 Part C: Chromosomes most of the time, genetic material is spread out into long, thin strands called chromatin when cell division starts, chromatin condenses to form chromosome o rod-shaped structures made of DNA tightly coiled around proteins called histones in cells preparing to divide, DNA has been duplicated to produce two identical chromatids chromatids are joined at the centromere Chromosomes numbers are constant within a species: o dog 78 o lettuce o fruit fly 8 o gorilla o human 46 o soybean 18 48 40 o o o potato 48 camel 70 guinea pig 64 Types of Chromosomes 1. sex chromosomes: one pair of chromosomes that determine gender ex. humans female= XX male= XY 2. autosomes: all of the remaining non-sex chromosomes ex.- 22 pairs of chromosomes having the same shape and carrying the same kinds of traits - one autosome is inherited from each parent karyotype = Photomicrograph of chromosomes in a dividing cell. In every cell but sex cells, there are distinct numbers of pairs of chromosomes: ex. during normal cell division: This number, which is typical of the species, is the diploid number (2n) During sex formation, this number is halved and is called the haploid (n) number Why is this necessary? If reproducing cells were diploid, then the new organism would have too many chromosomes. 5 Part D: Mitosis Cell Division in prokaryotes = binary fission is the division of the cell into two new daughter cells o each daughter cell has exactly the same kind and amount of DNA as the original cell had in eukaryotes = mitosis and/or meiosis (reduction division) Cell Cycle sequence of events in cell division: o Interphase o Mitosis o Cytokinesis Interphase resting stage cell spend most of their time in this phase (up to 90%) chromosomes not visible- in form of chromatin cell prepares for division o G1: cell grows o S (synthesis): DNA is copied o G2: cell prepares to divide Mitosis (M Phase) division of nucleus duplicated DNA evenly distributed to two daughter cells 1) Prophase 2) chromatin thickens and shortens into chromosome (now visible) centrioles migrate to opposite poles spindle fibers form nuclear membrane disappears Metaphase chromosomes line up at middle of cell centromeres divide; chromatids separate chromosomes easiest to identify in this phase 3) Anaphase chromatids (daughter chromosomes) move to opposite poles of the dividing cell pulled by traction fibers attached to centromeres 4) Telophase spindle disappears chromosomes become chromatin nuclear membranes reform Remember… People Meet 6 And Talk Cytokinesis division of cytoplasm Cancer mitosis controlled by proteins produced by oncogenes mutation in these proteins causes cancer abnormal cell growth causes tumors o benign: mass of “normal” cells o malignant: mass of cancerous cells metastasis- spread of cancer cells beyond their original site Fill in each box. centrioles chromatin interphase prophase spindle fibers metaphase anaphase telophase cytokinesis 10. 11. 12. 13. 14. 15. In humans, each cell (except sex cells) has how many chromosomes? 46 After mitosis, how many daughter cells are produced? 2 After mitosis (in a human cell), each daughter cell has how many chromosomes? 46 How many phases are in MITOSIS? 4 (Prophase, Metaphse, Anaphase, Telophase) Which phase of the cell cycle is the longest? interphase During which phase does cytokinesis begin? telophase 7 The diagram below shows six cells in various phases of the cell cycle. Note the cells are not arranged in the order in which mitosis occurs and one of the phases of mitosis occurs twice. Use the diagram to answer questions 1-7. 1) Cells A and F show an early and a late stage of the same phase of mitosis. What phase is it? prophase 2) Which cell is in metaphase? C 3) Which cell is in the first phase of mitosis? A 4) In cell A, what structure is labeled X? centrioles 5) Which cell is in the “in between” phase of mitosis? interphase 6) Place the diagrams in order from first to last. D A F C E B 7) Are the cells depicted plant or animal cells? Explain your answer. animal cells; they’re round and have centrioles. 8) What is the longest phase of the cell cycle? interphase 9) Why is mitosis important? growth & repair Practice Questions _____ 1. Which process is represented by the photographs below? 1. mitotic cell division 3. internal fertilization 2. zygote formation 4. segregation and recombination _____ 2. The diagrams below represent stages of a cellular process. 1. A B CD 2. B D C A 3. C B D A _____ 3. Normal mitotic cell division results in each daughter cell having 1. half the number of chromosomes as the parent cell 2. the same number and kinds of chromosomes as the parent cell 3. the same number but different kinds of chromosomes as the parent cell 4. twice the number of chromosomes as the parent cell 8 4. D B A C _____ 4. Which statement best describes the process of asexual reproduction? 1. It involves two parents. 3. It results in variation in offspring. 2. It occurs without the fusion of nuclei. 4. It involves the production of gametes. _____ 5. A photomicrograph of cells involved in various stages of nuclear division is shown at the right. Which title is most appropriate for this photomicrograph? 1. Mitosis in an Onion Root Tip 2. Cell Division in Human Blood Cells 3. Meiosis in Male Gametes 4. Gametogenesis in Yeast Cells _____ 6. Which would most likely result if mitosis was not accompanied by cytoplasmic division? 1. two cells, each with one nucleus 3. one cell with two identical nuclei 2. two cells, each without a nucleus 4. one cell without a nucleus _____ 7. The diagram illustrates the process of cell division. What is the significance of anaphase in this process? 1. Anaphase usually ensures that each daughter cell has the same number of chromosomes as the parent cell. 2. Anaphase usually ensures that each daughter cell has twice as many chromosomes as the parent cell. 3. In anaphase, the cell splits in half. 4. In anaphase, the DNA is being replicated. haploid haploid egg sperm diploid Part E: Meiosis Sexual Reproduction two parents genetically different offspring fusion of two gametes (sex cells) zygote = fertilization egg + sperm = zygote 23 + 23 = 46 value of variation is to allow species to adapt rapidly to new conditions o Egg and Sperm Production = gametogenesis 1. spermatogenesis = development of 4 sperm from each spermatogonial cell 2. oogenesis = development of 1 egg and 3 polar bodies from each oogonial cell Why? Unequal sharing of cytoplasm during cell division Fertilization restores diploid (2n) number of chromosomes one homologous chromosome comes from each parent voila! Variety!! 9 Meiosis cell division producing 4 cells with half the number of chromosomes (sperm & egg only) normal chromosome number restored at fertilization Meiosis I in interphase I, DNA replicates in prophase I chromosomes become visible, homologous chromosomes synapse (coming together) forming a tetrad in metaphase I, tetrads move to center of cell crossing-over may occur at this point (exchange of chromosomal segments) o allows genetic recombination anaphase I results in homologous chromosomes separating and moving to opposite poles of cell (chromatids do not separate) telophase I results in two daughter cells that are haploid 10 Meiosis II no replication prophase II- short or absent metaphase II- chromosomes to center of cell anaphase II- chromatids separate and move to poles telophase II- 4 cells form (haploid) nondisjunction- failure of chromosomes to separate during meiosis 11 12 Phases of Meiosis Name of Phase Description 1. prophase I Homologous chromosomes pair up and form tetrad 2. anaphase I Spindle fibers move homologous chromosomes to opposite sides 3. telophase II Nuclear membrane reforms, cytoplasm divides, 4 daughter cells formed 4. metaphase II Chromosomes line up along equator, not in homologous pairs 5. metaphase I Crossing-over occurs 6. anaphase II Chromatids separate 7. metaphase I Homologs line up alone equator 8. telophase I Cytoplasm divides, 2 daughter cells are formed anaphase I metaphase I prophase II anaphase II metaphase II prophase I telophase I telophase II 13 Meiosis On each of the images, label the phase of meiosis 1. anaphase II 4. telophase II 7. metaphase II 14 2. cytokinesis I 3. metaphase I 5. telophase I 6. cytokinesis II 8. prophase I 9. prophase II 10. anaphase I 11. A cell with a diploid number of 20 undergoes meiosis. This will produce 4 daughter cells, each with 10 chromosomes. 12. Synapsis occurs during this phase: prophase I 13. Tetrads line up along the equator during this phase: metaphase I 14. At the end of meiosis I, 2 daughter cells are created. These daughter cells are [ diploid | haploid ]. 15. Meiosis occurs in what type of cells: gametes (sex cells) 1. Each body cell of a chimpanzee contains 48 chromosomes. How many chromosomes would normally be present in a gamete produced by this chimpanzee? 1. 24 2. 36 3. 48 4. 96 _____2. The diagram shows a sequence of events . Which processes are indicated by letters A and B? 1. meiotic cell division and fertilization 2. mitotic cell division and fertilization 3. meiotic cell division and cleavage 4. mitotic cell division and cleavage _____3. In animals that reproduce sexually, which is the correct sequence for the development of a new organism? 1. gametes – zygote – fertilization – embryo 3. zygote – gametes – fertilization – embryo 2. fertilization – zygote – gametes – embryo 4. gametes – fertilization – zygote – embryo _____4. Which diagram best represents part of the process of sperm formation in an organism that has a normal chromosome number of eight? 1. 2. 3. _____5. The diagram below shows a process that can occur during meiosis. 4. The most likely result of this process is 1. a new combination of inheritable traits that can appear in the offspring 2. an inability to pass either of these chromosomes on to offspring 3. a loss of genetic information that will produce a genetic disorder in the offspring 4. an increase in the chromosome number of the organism in which this process occurs Mitosis and Meiosis Worksheet 15 I. Draw the chromosomes in the cell as it undergoes Mitosis: a a A b A B b B Prophase Metaphase Anaphase Telophase Daughter Cells II. Draw the chromosomes in the cell as it undergoes Meiosis (same parent cell): Prophase I Metaphase I Anaphase II Telophase II Comparing Mitosis & Meiosis 16 Anaphase I Metaphase II Telophase I Prophase II Daughter Cells Meiosis Mitosis # of cells made 4 2 # of cell divisions 2 1 chromosome # haploid (n) diploid (2n) cell type gametes body cells function sexual reproduction growth & repair Practice Questions _____1. The members of a certain species of grass in a lawn are genetically identical. The best explanation for this observation is that the species most probably reproduces 1. by an asexual method 3. after pollination by a particular species of bee 2. after pollination by the wind 4. by identical sperm fertilizing the eggs _____2. In humans, which cell is produced most directly by mitotic cell division? 1. a sperm cell 2. a skin cell 3. an egg cell 4. a zygote _____3. The diagram shows a sequence of events. The sequence of events from rabbits to zygote is known as 1. sexual reproduction 2. fission 3. sporulation 4. vegetative propagation _____4. Complex organisms produce sex cells that unite during fertilization, forming a single cell known as 1. an embryo 2. a gamete 3. a gonad 4. a zygote _____5. A cell with a diploid chromosome number of 12 divided two times, producing four cells with six chromosomes each. The process that produced these four cells was most likely 1. internal fertilization 2. external fertilization 3. mitotic cell division 4. meiotic cell division _____6. Which phrase best describes the process represented in the diagram? 1. 2. 3. 4. germination of a pollen grain in a flower identical gametes being formed by mitotic cell division development of seeds in a ovule daughter cells being formed by mitotic cell division 17 _____7. A colony of red bacteria is allowed to reproduce for 16 generations. A scientist examines the colony at the end of this time and notes that all the individuals are almost identical in all characteristics. This evidence suggests that the bacteria 1. did not receive the proper nutrients 3. exchanged genetic material 2. reproduced sexually 4. reproduced asexually _____8. Which statement best explains the significance of meiosis in the evolution of a species? 1. Meiosis produces eggs and sperm that are alike. 2. Meiosis provides for chromosomal variation in the gametes produced by an organism. 3. Equal numbers of eggs and sperm are produced by meiosis. 4. The gametes produced by meiosis ensure the continuation of any particular species by asexual reproduction. _____9. The diagrams represent some events in a cell undergoing normal meiotic cell division. Which diagram below most likely represents a new cell resulting from meiotic cell division of the cell? 1. 2. 3. 4. _____10. One way to produce large numbers of genetically identical offspring is by 1. cloning 3. changing genes by agents such as radiation or chemicals 2. fertilization 4. inserting a DNA segment into a different DNA molecule _____11. How does the type of reproduction shown in method A in the diagram differ from the type of reproduction shown in method B ? 1. Method A illustrates sexual reproduction, and method B illustrates asexual reproduction. 2. Offspring produced by method B will be genetically alike, but offspring produced by method A will be genetically different. 3. The two cells shown in the last step of method A are genetically alike, but the two cells shown in the last step of method B are genetically different. 4. Offspring produced by method A will be genetically like the parent, but offspring produced by method B will be genetically different from the parents. _____12. The nucleus is removed from a body cell of one organism and is placed in an egg cell that has had its nucleus removed. This process, which results in the production of organisms that are genetically alike, is known as 1. cloning 2. fertilization 3. biological adaptation 4. DNA production 18 _____13. Most cells in the body of a fruit fly contain eight chromosomes. In some cells, only four chromosomes are present, a condition which is a direct result of 1. mitotic cell division 2. meiotic cell division 3. embryonic differentiation 4. internal fertilization _____14. The chromosome content of a skin cell that is about to form two new skin cells is represented in the diagram. Which diagram below best represents the chromosomes that would be found in the two new skin cells produced as a result of this process? 1. 3. 2. 4. _____15. Strawberries can reproduce by means of runners, which are stems that grow horizontally along the ground. At the region of the runner that touches the ground, a new plant develops. The new plant is genetically identical to the parent because 1. it was produced sexually 2. nuclei traveled to the new plant through the runner to fertilize it 3. it was produced asexually 4. there were no other strawberry plants in the area to provide fertilization _____16. Which statement is true of both mitosis and meiosis? 1. Both are involved in asexual reproduction. 3. The number of chromosomes is reduced by half. 2. Both occur only in reproductive cells. 4. DNA replication occurs before the division of the nucleus. _____17. Meiosis and fertilization are important for the survival of many species because these two processes result in 1. large numbers of gametes 3. cloning of superior offspring 2. increasingly complex multicellular organisms 4. genetic variability of offspring _____18. The least genetic variation will probably be found in the offspring of organisms that reproduce using 1. mitosis to produce a larger population 2. meiosis to produce gametes 3. fusion of eggs and sperm to produce zygotes 4. internal fertilization to produce an embryo 19 _____19. Which row in the chart below best describes asexual reproduction? Number of Parents Comparison of Offspring to Parents 1. one identical 2. one different 3. two identical 4. two different Row _____20. Marine sponges contain a biological catalyst that blocks a certain step in the separation of chromosomes. Which cellular process would be directly affected by this catalyst? 1. mitosis 2. diffusion 3. respiration 4. photosynthesis _____21. Which two structures of a frog would most likely have the same chromosome number? 1. skin cell and fertilized egg cell 3. kidney cell and egg cell 2. zygote and sperm cell 4. liver cell and sperm cell _____22. The diagram to the left represents a nucleus containing the normal chromosome number for a species. Which diagram below bests illustrates the normal formation of a cell that contains all of the genetic information needed for growth, development, and future reproduction of this species? 1. 2. 3. 4. _____23. Which statement best explains the significance of meiosis in the process of evolution within species? 1. 2. 3. 4. 20 The gametes produced by meiosis ensure the continuation of any particular species by asexual reproduction. Equal numbers of eggs and sperm are produced by meiosis. Meiosis produces eggs and sperm that are alike. Meiosis provides for variation in the gametes produced by an organism. 21