Characterization and Primary Functional Analysis of Phenylalanine Ammonia-Lyase Gene from Phyllostachys edulis Z.M. Gaoa* · X.C. Wanga · Z.H. Penga,b* · B. Zhenga · Q. Liua a International Center for Bamboo and Rattan, State Forestry Administration Key Open Laboratory on Bamboo and Rattan Science and Technology, Beijing 100102, P. R. China b Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China Corresponding author. Zhimin Gao, Tel.: +86-10-84789802; fax: +86-10-84789803, E-mail address: firstname.lastname@example.org *Co-Corresponding author. Zhenhua Peng, E-mail address: email@example.com ESM_2_ HPLC Analysis of trans-Cinnamic Acid Produced by a PAL Reaction Using Purified Recombinant PePAL. Absorbance at 278 nm (A) (B) (C) Retention Time (min) A standard solution (6.7 mM) of trans-cinnamic acid (10 μL) was analyzed at 278 nm (A). The results of the PAL reactions, which were carried out with either the protein fraction purified from E. coli harboring an empty pET-30a vector (B) or the purified recombinant PePAL (C), are also shown. The arrowheads indicate the peaks of trans-cinnamic acid.