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ANTINEOPLASTONS – HOW THEY WORK
BACKGROUND
Antineoplastons are naturally occurring peptides and amino acid derivatives, which control neoplastic growth.
Research on peptides involved in cell-to-cell communication has grown at an impressive pace. Most of the
effort has been concentrated in identification of the peptide growth factors, which by 1995 led to the discovery
of approximately a hundred growth factors. Much less activity has focused on the isolation of peptide growth
inhibitors. Thirty years ago, when the research on antineoplastons was started, very few researchers was
interested in biologically active peptides and only a few such peptides had been identified, including pituitary
hormones, plasma kinins, and angiotensins.
Neoplastic process is a disease of cell differentiation. Given the large number of differentiating cells and
assuming the possibility of error in the program of differentiation, groups of abnormally growing cells can arise
very often under the influence of carcinogenic factors. Without a reliable mechanism, which can induce normal
differentiation in cancer cells, chances for the survival of the organism will be very small. Such a corrective
system can reprogram the growth of newly developed neoplastic cells and direct them into normal
differentiation pathways. In the case of failure of this line of defense, the organism will not be able to defend
itself against chemical and physical carcinogens, viruses and other neoplastic growth inducing agents, and will
develop cancer.
We have proposed the name "antineoplastons" for the components of such a redifferentiation system, which
exists in the human organism. According to our definition, antineoplastons are the substances produced by the
living organism which protect it against the development of neoplastic growth by a non- immunological process
that does not significantly inhibit the growth of normal tissues. Based on theoretical grounds, it is very likely
that antineoplastons have a peptide or amino acid derivative structure. Assuming that an error in the program for
normal cell differentiation is responsible for neoplastic growth, the components of such a biochemical defense
system against cancer should be information carrying molecules. Because of their high information content,
peptides are ideal compounds to participate in such a line of defense.
The theory of antineoplastons was conceived through the application of the cybernetic theory of autonomous
systems to studies of peptides in human blood. The ideal cancer treatment should provide a large amount of
information and a small amount of energy. Such a treatment may control neoplastic growth by directing the
cancer cells into normal channels of differentiation. The practically infinite variety of peptides that can be
formed by the combination of the twenty common amino acids indicates the possibility of the existence of a
peptide system carrying information from cell to cell that is able to correct errors in the program for
differentiation.
The study of antineoplastic peptides existing in human blood and urine began in 1967. During the analysis of
peptides and amino acids in the blood of healthy people and of patients suffering from various diseases,
including cancer, it was noticed that there are significant differences in the peptide content in serum of cancer
patients as compared with the control group. In the next few years, it was found that similar peptide fractions
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exist in urine. It was postulated that these peptides are probably synthesized in the tissues and passed into the
blood and urine. Urine was proposed as the most economic source for their isolation and, therefore, a research
program was established for the isolation and identification of antineoplastic peptides from human urine.
Medicinal use of urine and urine extracts has been known for centuries. Serapion from Alexandria (III c.B.C.)
was probably the most famous Greek physician to apply medicines derived from urine, but his experience was
based on earlier Egyptian knowledge. Greek physicians practicing in ancient Rome were also very familiar with
this type of treatment. Such therapy was described by Galen who is credited with the first medical description of
cancer and also introducing the name "cancer." However, the most famous Greek in Rome in the second
century A.D. to use urine therapy was Xenokrates. Medicinal use of urine was also known in ancient India, the
importance of which is well described in a Sanskrit text entitled "Shivambu-kalp". Specifically, it should be
mentioned that the treatment of cancer with urine was also then known in India. The interest in urine from both
the therapeutic and diagnostic points of view persisted during the Middle Ages in Europe. "Essentia urinae "
was commonly used at that time, and the vessel for urine "matula" was as widely shown by painters as an
emblem of the practice of medicine as a cane with a snake in antiquity and the stethoscope in modern time’s.
Medicine men in North and Central America were also quite familiar with urine treatment for various
conditions, including cancer.
In modern times, the first approach to the study of growth inhibiting substances in urine was conducted in 1937.
Rohdenburg and Nagy, after studying samples from 541 cases, described the method of isolation of growth
inhibiting agents from human urine. Floyd C. Turner in 1939 went one step further and tested the effect of
Rohdenburg and Nagy's preparation on tumors in mice. He was able to find that a growth inhibiting substance
derived from human urine prevented the formation of tumors in mice following injections of carcinogens, but
the urinary derivative had little specific inhibiting effect on transplanted or spontaneous tumors in mice.
Magnelia, Kaplan, and Hyson in 1943 were among the first who tried to treat human cancer by urine injections.
In several cases of inoperable cancer, treated by daily urine injections and observed up to 18 months, they
noticed marked relief of symptoms with no side effects. Von Wollheim prepared in 1948 an ether extract from
human urine, which showed an inhibitory effect on cancer in mice. Considerable research, both basic and
clinical, was done in England and Germany on a polypeptide of unknown structure named H-11, which was
isolated from urine. In the initial clinical studies, 16 cases of inoperable lung cancer were treated by injections
of urine extract. Relief of symptoms, e.g., cough, improvement of the general condition and prolongation of life
in comfort was observed in most cases without any significant side effects. In more extensive trials described in
1968 by von Klose and Below, 49 cases of inoperable lung cancer were treated by injections of H-11 extract in
combination with chemotherapy. Combination therapy gave better results than treatment with urine extract or
chemotherapy alone. H-11 extract contained polypeptides, which were chemically different from
antineoplastons, as proved by our research and the analysis done by the manufacturer of H-11.
Antineoplastons are also completely different from peptides and amino acid derivatives isolated from urine by
the other authors. Investigations on peptides in urine began 97 years ago and were initiated by Polish researcher
Bondzynski. Further studies were continued by Bondzynski's former assistants in the University of Lwow and
University of Warsaw. These compounds, initially named oxyproteic acids, were proven to be a wellcharacterized group of peptides by Skarzynski and Sarnecka-Keller.
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