James D. Tucker
Professor and Chair
B.S. Genetics, University of California at Davis, 1976. Ph.D., Human Genetics, Oregon Health
Sciences University, 1981. Joined WSU Faculty August, 2002.
Department of Biological Sciences
1370 Biological Sciences Building
5047 Gullen Mall
Wayne State University
Detroit, MI 48202-3917
Office phone: (313) 577-2783
Office fax: (313) 577-3602
Departmental office phone: (313) 577-2873
e-mail: jtucker@biology.biosci.wayne.edu
RESEARCH GOALS:
My research is focused on understanding, characterizing and quantifying the biological
effects of environmental exposure to chemicals and ionizing radiation, and identifying the
molecular mechanisms involved in oncogenic processes.
Two research efforts are ongoing in my laboratory. The goal of the first project is to
produce a mobile radiation biodosimetry system for field deployment. This is being
accomplished by developing and incorporating technologies in biology as well as engineering.
The system will incorporate DNA probes which replicate the mRNA products of specified
radiosensitive (target) genes amplified from individual human blood cells via a molecular
reaction known as rolling circle amplification (RCA). The technology will be incorporated in a
measuring device (optimally hand held) which uses fluorescence to quantify the mRNA
products of the specified target genes. The degree of fluorescence produced by the RCA
reaction will be related to the amount of ionizing radiation absorbed by the cells.
The goal of the second project is to identify and characterize genetic changes associated
with tumor progression and metastasis. Many types of tumors are comprised of subpopulations
of cells that differ as a result of clonal expansion and concomitant accumulation of genetic
changes including structural and numerical chromosome aberrations and point mutations. This
tumor cell “evolution” creates a diversity of population sub-types with different potentials for
invasion of nearby tissues or metastasis to distant sites. While treatment with chemotherapy or
radiation therapy shrinks tumor mass by cell killing, some cells survive and many of these have
accumulated additional mutations as a result of treatment. The surviving cells may have greater
resistance to therapy and exhibit an associated growth advantage with adverse consequences to
the patient. Understanding and characterizing these changes is critical to our understanding of
tumor cell biology and is expected to be foundational to improving cancer therapies.
HONORS AND AWARDS:
Recipient of a 1994 R&D 100 Award for "Chromosome-Specific DNA Probes for the Common
Laboratory Mouse".
Recipient of a 2001 R&D 100 Award for "Gene Recovery Microdissection".
Recipient of a 2002 R&D 100 Award for “In Situ Rolling Circle Amplification”.
PATENTS:
"Amplification of Chromosomal DNA In Situ," U.S. Patent No.: 6,432,650, October, 2002.
"In Situ Rolling Circle Amplification to Detect Single Base Changes and Gene Expression
Levels in Single Cells," patent pending November, 2001.
RECENT PUBLICATIONS (peer-reviewed):
Jones, I.M., Galick, H., Kato, P., Langlois, R.G., Mendelsohn, M.L., Murphy, G.A., Pleshanov,
P., Ramsey, M.J., Thomas, C.B., Tucker, J.D., Tureva, L., Vorobtsova, I., and Nelson,
D.O. (2002) Three somatic genetic biomarkers and covariates in radiation exposed
Russian clean-up workers of the Chernobyl nuclear reactor , 6 – 13 years after exposure.
Radiation Research 158:424-442.
Gardner, S.N. and Tucker, J.D. (2002) The cellular lethality of radiation-induced chromosome
translocations in human lymphocytes. Radiation Research 157:539-552.
Tucker, J.D. (2002) Sensitivity, specificity, and persistence of chromosome translocations for
radiation biodosimetry. Military Medicine 167, Suppl. 1:8-9.
Christian, A.T., Pattee, M.S., Attix, C.M., Reed, B.E., Sorensen, K.J. and Tucker, J.D. (2001)
Detection of DNA point mutations and mRNA expression levels by rolling circle
amplification in individual cells. Proceedings of the National Academy of Sciences
98:14,238-14,243.
Jones, I.M., Tucker, J.D., Langlois, R.G., Mendelsohn, M.L., Pleshanov, P., Nelson, D.O. (2001)
Evaluation of three somatic genetic biomarkers as indicators of low-dose radiation effects
in cleanup workers of the Chernobyl nuclear reactor accident. Radiation Protection
Dosimetry 97: 61-67.
Tucker, J.D. (2001) FISH cytogenetics and the future of radiation biodosimetry. Radiation
Protection Dosimetry 97:55-60.
Sorensen, K.J., Zetterberg, L.A., Nelson, D.O., Grawe, J., and Tucker, J.D. (2000) The in vivo
dose rate effect of chronic gamma radiation in mice: translocation and micronucleus
analyses. Mutation Research 457:125-136.
Pluth, J.M., Nelson, D.O., Ramsey, M.J., and Tucker, J.D. (2000) The relationship between
genotype and chromosome aberration frequencies in a normal adult population.
Pharmacogenetics 10:311-319.
Tucker, J.D. (2000) Evaluation of chromosome translocations by FISH for radiation
biodosimetry: A view from one laboratory. Radiation Protection Dosimetry 88:87-92.
Pluth, J.M., Ramsey, M.J., and Tucker, J.D. (2000) Role of maternal exposures and newborn
genotypes on newborn chromosome aberration frequencies. Mutation Research 465:101111.
Spruill, M.D., Nelson, D.O., Ramsey, M.J., Nath, J., and Tucker, J.D. (2000) Lifetime
persistence and clonality of chromosome aberrations in the peripheral blood of mice
acutely exposed to ionizing radiation. Radiation Research 153:110-121.
NOTE: THE FOLLOWING SHOULD BE ACCESSIBLE BY CLICKING ON A BUTTON THAT
SAYS SOMETHING TO THE EFFECT OF “CLICK HERE FOR MORE REFERENCES”
OLDER PUBLICATIONS (peer-reviewed):
Aksoy, I.A., Ramsey, M.J., Fruman, D.A., Aksoy, S., Cantley, L.C., Tucker, J.D., and Roberts,
T.M. (1999) Mouse phosphoinositide 3-kinase p100a gene: Cloning, structural
organization and localization to chromosome 3 band B. Biochemical and Biophysical
Research Communications 262: 438-442.
Moore II, D.H. and Tucker J.D. (1999) Biological dosimetry of Chernobyl clean-up workers:
Inclusion of age and smoking data provide improved radiation dose estimates. Radiation
Research 152:655-664.
Radivoyevitch, T., Ramsey, M.J., and Tucker, J.D. (1999) Estimation of the target stem cell
population size in chronic myeloid leukemogenesis. Radiation and Environmental
Biophysics 38:201-206.
Gabriel, J.M., Merchant, M., Ohta, T., Ji, Y., Caldwell, R.G., Ramsey, M.J., Tucker, J.D.,
Longnecker, R., Nicholls, R.D. (1999) A transgene insertion creating a heritable
chromosome deletion mouse model of Prader-Willi and Angelman syndromes.
Proceedings of the National Academy of Sciences 96:9258-9263.
Johnson, K.L., Nath, J., Geard, C.R., Brenner, D.J., and Tucker, J.D. (1999) Chromosome
aberrations of clonal origin in irradiated and unexposed individuals: assessment and
implications. Radiation Research 152:1-5.
Christian, A.T., Garcia, H.E. and Tucker, J.D. (1999) Extending the useful lifespan of DNA
probes for fluorescence in situ hybridization. BioTechniques 26:1068-1072.
Christian, A.T., Garcia, H.E., and Tucker, J.D. (1999) PCR in situ followed by microdissection
allows whole chromosome painting probes to be made from single microdissected
chromosomes. Mammalian Genome 10:628-631.
Tucker, J.D., Spruill, M.D., Ramsey, M.J., Director, A.D., and Nath, J. (1999) Frequency of
spontaneous chromosome aberrations in mice: Effects of age. Mutation Research
425:135-141.
Johnson, K.L., Brenner, D.J., Nath, J., Tucker, J.D., and Geard, C.R. (1999) Radiation-induced
breakpoints in human chromosomes: random or non-random? International Journal of
Radiation Biology 75:131-141.
Johnson, K.L., Nath, J., Pluth, J., and Tucker, J.D. (1999) The distribution of chromosome
damage, non-reciprocal translocations and clonal aberrations in lymphocytes from
Chernobyl clean-up workers. Mutation Research 439:77-85.
Matsumoto, K., and Tucker, J.D. (1998) Detection of structural chromosome damage in rat
interphase cells using region-specific fluorescence in situ hybridization probes developed
by microdissection. Mutation Research 421:191-196.
Nakamura, N., Tucker, J. D., Bauchinger, M. Littlefield, L.G., Lloyd, D.C., Preston, R.J., Sasaki,
M.S., Awa, A.A., and Wolff, S. (1998) Meeting Report: F values as cytogenetic
fingerprints of prior exposure to different radiation qualities: Prediction, reality, and
future. Radiation Research 150:492-494.
Director, A.E., Tucker, J.D., Ramsey, M.J., and Nath, J. (1998) Chronic ingestion of clastogens
by mice and the frequency of chromosome aberrations. Environmental and Molecular
Mutagenesis 32:139-147.
Tucker, J.D., Sorensen, K.J., Chu, C.S., Nelson, D.O., Ramsey, M.J., Urlando, C., and Heddle,
J.A. (1998) The accumulation of chromosome aberrations and Dlb-1 mutations in mice
with highly fractionated exposure to gamma radiation. Mutation Research 400:321-335.
Littlefield, L.G., McFee, A.F., Salomaa, S.I., Tucker, J.D., Inskip, P.D., Sayer, A.M., Lindholm,
C., Makinen, S., Mustonen, R., Sorensen, K., Tekkel, M., Veidebaum, T., Auvinen, A.,
and Boice Jr., J.D. (1998) Do recorded doses overestimate true doses received by
Chernobyl cleanup workers? Results of cytogenetic analyses of Estonian workers by
fluorescence in situ hybridization. Radiation Research 150:237-249.
Ramsey, M.J., Nagao, M., Inoue, R., Fujita, H., Matsushima, T., and Tucker, J.D. (1998)
Chromosome aberrations induced in mice by chronic feeding of 2-amino-3,4dimethylimidazo[4,5-f]quinoline (MeIQ). Food and Chemical Toxicology 36:467-474.
Johnson, K.L., J.D. Tucker, and J. Nath. (1998) Frequency, distribution and clonality of
chromosome damage in human lymphocytes by multi-color FISH. Mutagenesis 13:217227.
Liu, N., Lamerdin J.E., Tebbs R.S., Schild, D., Tucker, J.D., Shen, M.R., Brookman, K.W.,
Siciliano, M.J., Walter C.A. Fan, W., Narayana, L.S., Zhou, Z-Q., Adamson A.W.,
Sorensen, K.J., Chen, D.J., Jones, N.J., and Thompson L.H., (1998) XRCC2 and XRCC3,
new human Rad51-family members, promote chromosome stability and protect against
DNA crosslinks and other damages. Molecular Cell 1:783-793.
Matsumoto, K., Ramsey, M.J., Nelson, D.O. and Tucker, J.D. (1998) Persistence of radiationinduced translocations in human peripheral blood determined by chromosome painting.
Radiation Research 149:602-613.
Tucker, J.D. (1998) The use of chromosome translocations for measuring prior environmental
exposures in humans. In: Biomarkers: Medical and Workplace Applications, pp. 117-132.
M. Mendelsohn, L. Mohr and J. Peeters, eds. Joseph Henry Press, Washington D.C.
Steenland, K., Cedillo, L., Tucker, J., Hines, C., Sorensen, K., Deddens, J., Cruz, V. (1997)
Thyroid hormones and cytogenetic outcomes in backpack sprayers using
ethylenebis(dithiocarbamate) (EBDC) fungicides in Mexico. Environmental Health
Perspectives 10:1126-1130.
Tucker, J.D., Breneman, J.W., Briner, J.F., Eveleth, G.G., Langlois, R.G., and Moore II, D.H.
(1997) The persistence of radiation-induced translocations in rat peripheral blood
determined by chromosome painting. Environmental and Molecular Mutagenesis 30:264272.
Kadkhodayan, S., Salazar, E.P., Ramsey, M.J., Takayama, K., Zdzienicka, M.Z., Tucker, J.D.,
Weber, C.A. (1997) Molecular analysis of ERCC2 mutations in the repair deficient
hamster mutants UVL-1 and V-H1. Mutation Research 385:47-57.
Moore II, D.H., Tucker, J.D., Jones, I.M., Langlois, R.G., Pleshanov, P., Vorobtsova, I., and
Jensen, R. (1997) A study of the effects of exposure on cleanup workers at the Chernobyl
nuclear reactor accident using multiple end points. Radiation Research 148:463-475.
Hando, J.C., Tucker, J.D., Davenport, M., Tepperberg, J., Nath, J. (1997) X chromosome
inactivation and micronuclei in normal and Turner individuals. Human Genetics,
100:624-628.
Tucker, J.D., Eastmond, D.A., Littlefield, L.G. (1997) Cytogenetic endpoints as biological
dosimeters and predictors of risk in epidemiological studies. In: “Application of
Biomarkers to Cancer Epidemiology”; pp. 185-200. P. Toniolo, P. Boffetta, D.E.G.
Shuker, N. Rothman, B. Hulka, and N. Pearce, ed. IARC Scientific Publication No. 142.
Liu, N., Lamerdin, J.E., Tucker, J.D., Zhou, Z.-Q., Walter, C.A., Albala, J.S., Busch, D.B., and
Thompson, L.H. (1997) The human XRCC9 gene corrects chromosomal instability and
mutagen sensitivities in CHO UV40 cells. Proc. Nat’l Acad. Sci., 94:9232-9237.
Tucker, J.D., Tawn, E.J., Holdsworth, D., Morris, S., Langlois, R., Ramsey, M.J., Kato, P.,
Boice, J.D. Jr., Tarone, R.E., Jensen, R.H. (1997) Biological dosimetry of radiation
workers at the Sellafield Nuclear Facility. Radiation Research 148:216-226.
MacGregor, J.T., Wehr, C.M., Hiatt, R.A., Peters, B., Tucker, J.D., Langlois, R. Jacob, R.A.,
Jensen, R.H., Yager, J.W., Shigenaga, M.K., Frei, B., Eynon, B.P., and Ames, B.N.
(1997) “Spontaneous” genetic damage in man: evaluation of interindividual variability,
relationship among markers of damage, and influence of nutritional status. Mutation
Research, 377:125-135.
Okonogi, H., Ushijima, T., Zhang, X.B., Heddle, J.A., Suzuki, T., Sofuni, T., Felton, J.S.,
Tucker, J.D., Sugimura, T., and Nagao, M. (1997) Agreement of mutational
characteristics of heterocyclic amines in lacI of the Big Blue mouse with those in tumor
related genes in rodents. Carcinogenesis 18:745-748.
Wu, R.W., Tucker, J.D., Sorensen, K.J., Thompson, L.H., and Felton, J.S. (1997) Differential
effect of acetyltransferase expression on the genotoxicity of heterocyclic amines in CHO
cells. Mutation Research 390:93-103.
Inskip, P.D., Hartshorne, M.F., Tekkel, M., Rahu, M., Veidebaum, T., Auvinen, A., Crooks,
L.A., Littlefield, L.G., McFee, A.F., Salomaa, S., Makinen, S., Tucker, J.D., Sorensen,
K.J., Bigbee, W.L., Boice, J.D. (1997) Thyroid nodularity and cancer among Chernobyl
clean-up workers from Estonia. Radiation Research 147:225-235.
Inskip, P.D., Tekkel, M., Rahu, M., Veidebaum, T., Hakulinen, T., Auvinen, A., Rytomaa, T.,
Servomaa, K., Obrams, G.I., Stengrevics, A., Kesminiene, A-Z., Kurtinaitis, J.,
Hartshorne, M.F., Littlefield, L.G., Salomaa, S., Tucker, J.D., Bigbee, W.L., Jensen,
R.H., Moloney, W.C., Boice Jr., J.D. Studies of leukemia and thyroid disease among
Chernobyl clean-up workers from the Baltics. In Boice JD Jr. (Ed): Implications of New
Data on Radiation Cancer Risk. Proceedings of the Thirty-Second Annual Meeting of the
National Council on Radiation Protection and measurements. Proceedings No. 18.
National Council on Radiation Protection and Measurements: Bethesda, Maryland, 1997,
pp. 123-141.
Savage, J.R.K. and Tucker, J.D. (1996) Nomenclature systems for FISH-painted chromosome
aberrations. Mutation Research 366:153-161.
Zhang, X.B., Felton, J.S., Tucker, J.D., Urlando, C., and Heddle,. J.A. (1996) Intestinal
mutagenicity of two food mutagens in transgenic mice: 2-amino-1-methyl-6phenylimidazo[4,5-b]pyridine (PhIP) and amino(a)carboline. Carcinogenesis 17:22592265.
Breneman, J.W., Briner, J.F., Ramsey, M.J., Director, A., and Tucker, J.D. (1996) Cytogenetic
results from a chronic feeding study of MeIQx in mice. Food and Chemical Toxicology
34:717-724.
Tucker, J.D. and Preston, R.J. (1996) Chromosome aberrations, micronuclei, aneuploidy, SCEs
and cancer risk assessment. Mutation Research 365:147-159.
Spruill, M.D., Ramsey, M.J., Swiger, R.R., Nath, J. and Tucker, J.D. (1996) The persistence of
aberrations in mice induced by gamma radiation as measured by chromosome painting.
Mutation Research 356:135-145.
Breneman, J.W., Yau, P.M., Swiger, R.R., Teplitz, R., Smith, H.A., Tucker, J.D., and Bradbury,
E.M. (1996) Activity banding of human chromosomes as shown by histone acetylation.
Chromosoma 105:41-49.
Swiger, R.R. and Tucker, J.D. (1996) Fluorescence in situ hybridization: a brief review.
Environmental and Molecular Mutagenesis 27:245-254.
Tucker, J.D. and Moore II, D.H. (1996) The importance of age and smoking in evaluating
adverse cytogenetic effects of exposure to environmental agents. Environmental Health
Perspectives 104(suppl. 3):489-492.
Tucker, J.D., Nath, J. and Hando, J.C. (1996) Activation status of the X chromosome in human
micronucleated lymphocytes. Human Genetics 97:471-475.
Director, A.E., Nath, J., Ramsey, M.J., Swiger, R.R. and Tucker, J.D. (1996) Cytogenetic
analysis of mice chronically fed the food mutagen 2-amino-1-methyl-6phenylimidazo[4,5b] pyridine (PhIP). Mutation Research 359:53-61.
Caldecott, K.W., Tucker, J.D., Stanker, L.H. and Thompson, L.H. (1995) Characterization of the
XRCC1-DNA ligase III complex in vitro and its absence from mutant hamster cells.
Nucleic Acids Research 23:4836-4843.
Wilkinson, D., Sandhu, J.K., Breneman, J.W., Tucker, J.D. and Birnboim, H.C. (1995) Hprt
mutants in a transplantable murine tumour arise more frequently in vivo than in vitro. Br.
J. Cancer 72:1234-1240.
Heddle, J.A., Tao, K., Swiger, R.R. and Tucker, J.D. (1995) The transmission rate of the lacI
transgene from the Big Blue‘ mouse. Mutation Research, 348:63-66.
Ramsey, M.J., Moore II, Dan H., Briner, J.F., Lee, D. A., Olsen, L.A., Senft, J.R., and Tucker,
J.D. (1995) The effects of age and lifestyle factors on the accumulation of cytogenetic
damage as measured by chromosome painting. Mutation Research, 338:95-106.
Nath, J., Tucker, J.D., Hando, J.C. (1995) Y chromosome aneuploidy, micronuclei, kinetochores
and aging in men. Chromosoma 103:725-731.
Tebbs, R.S., Zhao, Y., Tucker, J.D., Scheerer, J.B., Siciliano, M.J., Hwang, M., Liu, N.,
Legerski, R.J., and Thompson, L.H. (1995) Correction of chromosomal instability and
sensitivity to diverse mutagens by a cloned cDNA of the XRCC3 DNA repair gene. Proc.
Nat'l Acad. Sci., 92:6354-6358.
Tucker, J.D., Morgan, W.F., Awa, A.A., Bauchinger, M., Blakey, D., Cornforth, M.N.,
Littlefield, L.G., Natarajan, A.T., Shasserre, C. (1995) PAINT: A proposed nomenclature
for structural aberrations detected by whole chromosome painting. Mutation Research,
347:21-24.
MacGregor, J.T., Tucker, J.D. Eastmond, D.A., Wyrobek, A.J. (1995) Integration of cytogenetic
assays with toxicology studies. Environmental and Molecular Mutagenesis, 25:328-337.
MacGregor, J.T., Farr, S., Tucker, J.D., Heddle, J.A., Tice, R.R., and Turteltaub, K.W. (1995)
New molecular endpoints and methods for routine toxicity testing. Fundamental and
Applied Toxicology, 26:156-173.
Swiger, R.R., Tucker, J.D., and Heddle, J.A. (1995) A method for detecting transgenic animals
without cell culture using fluorescence in situ hybridization. Biotechniques 18:952-958.
Tucker, J.D., Lee, D.A., Moore II, D.H. (1995) Validation of chromosome painting II: A detailed
analysis of aberrations following high doses of ionizing radiation in vitro. Internat. J.
Rad. Biol. 67:19-28.
Tucker, J.D., Morgan, W.F., Awa, A.A., Bauchinger, M., Blakey, D., Cornforth, M.N.,
Littlefield, L.G., Natarajan, A.T., Shasserre, C. (1995) A proposed system for scoring
structural aberrations detected by chromosome painting. Cytogenetics and Cell Genetics
68:211-221.
Breneman, J.W., Swiger, R.R., Ramsey, M.J., Minkler, J.L., Eveleth, G.G., Langlois, R., Tucker,
J.D. (1995) The development of painting probes for dual-color and multiple chromosome
analysis in the mouse. Cytogenetics and Cell Genetics 68:197-202.
Swiger, R.R., Myhr, B., Tucker, J.D. (1994) The LacZ transgene in Muta™Mouse maps to
chromosome 3. Mutation Research 325:145-148.
Tucker, J.D., Lee, D.A., Ramsey, M.J., Briner, J., Olsen, L., and Moore II, D.H. (1994) On the
frequency of chromosome exchanges in a control population measured by chromosome
painting. Mutation Research 313:193-202.
Tucker, J.D., Senft, J.R. (1994) Analysis of naturally occurring and radiation-induced breakpoint
locations in human chromosomes 1, 2, and 4. Radiation Research 139:31-36.
Brookman, K.W., Tebbs, R.S., Allen, S.A., Tucker, J.D., Swiger, R.R., Lamerdin, J.E., Carrano,
A.V., and Thompson, L.H. (1994) Isolation and characterization of mouse Xrcc-1, a
DNA repair gene affecting ligation. Genomics 22:180-188.
Hando, J.C., Nath, J. and Tucker, J.D. (1994) Sex chromosomes, micronuclei and aging in
women. Chromosoma 103:186-192.
Matsuoka, A., Tucker, J.D., Hayashi, M., Yamazaki, N., and Sofuni, T. (1994) Chromosome
painting analysis of X-ray-induced aberrations in human lymphocytes in vitro.
Mutagenesis 9:151-156.
Caldecott, K.W., McKeown, C.K., Tucker, J.D., Ljungquist, S., and Thompson, L.H. (1994) An
interaction between the mammalian DNA repair protein XRCC1 and DNA ligase III.
Molecular and Cellular Biology 14:68-76.
Miyashita, K., Vooijs, M.A., Tucker, J.D., Lee, D.A., Gray, J.W., and Pallavicini M.G. (1994) A
mouse chromosome 11 library generated from sorted chromosomes using linker-adapter
polymerase chain reaction Cytogenetics and Cell Genetics 66:54-57.
Breneman, J.W., Ramsey, M.J., Lee, D.A., Eveleth, G.G., Minkler, J.L., and Tucker, J.D. (1993)
The development of chromosome-specific composite DNA probes for the mouse and
their application to chromosome painting. Chromosoma 102:591-598.
Steenland, K., Tucker, J., and Salvan, A. (1993) Problems in assessing the relative predictive
value of internal markers versus external exposure in chronic disease epidemiology.
Cancer Epidemiology, Biomarkers and Prevention 2:487-491.
Tucker, J.D., Ramsey, M.J., Lee, D.A, and Minkler, J.L. (1993) Validation of chromosome
painting as a biodosimeter in human peripheral lymphocytes following acute exposure to
ionizing radiation in vitro. International J. Rad. Biology 64:27-37.
Natarajan, A.T., Tucker, J.D., and Sasaki, M.S. (1993). Monitoring cytogenetic damage in vivo.
Scientific Group for the Safety Evaluation of Chemicals. Ed. R.G. Tardiff, P.H.M.
Lohman, and G.N. Wogan, In Methods to Assess DNA Damage and Repair: Interspecies
Comparisons. pp. 95-116.
Tucker, J.D., Auletta, A., Cimino, M.C., Dearfield, K.L., Jacobson-Kram, D., Tice, R.R., and
Carrano A.V. (1993) Sister-chromatid exchange: Second report of the Gene-Tox
program. Mutation Research 297:101-180.
Tucker, J.D., Vanderlaan, M., Kwan, T.C., Moore II, D.H., Felton, J.S. (1993). Effects of diet
and folate on levels of micronucleated polychromatic erythrocytes. Mutation Research
301:19-26.
Caldecott, K.W., Tucker, J.D., and Thompson, L.H. (1992) Construction of human XRCC1
minigenes that fully correct the CHO DNA repair mutant EM9. Nucleic Acids Research
20:4575-4579.
Straume, T., Lucas, J.N., Tucker, J.D., Bigbee, W.L., Langlois, R.G. (1992). Biodosimetry for a
radiation worker using multiple assays. Health Physics, 62:122-130.
Tucker, J.D., Jones, N.J., Allen, N.A., Minkler, J.L., Thompson, L.H., and Carrano, A.V. (1991).
Cytogenetic characterization of the ionizing radiation-sensitive Chinese hamster mutant
irs1. Mutation Research, 254:143-152.
Heddle, J.A., Cimino, M.C., Hayashi, M., Romagna, F., Vanparys, P., and MacGregor, J.T.
(1991) Micronuclei as an index of cytogenetic damage: Past, present, and future.
Environmental and Molecular Mutagenesis, 18:277-291.
Buonarati, M.H., Tucker, J.D., Minkler, J.L., Wu, R.W., Thompson, L.H., and Felton, J.S.
(1991). Metabolic activation and cytogenetic effects of 2-amino-1-methyl-6phenylimidazo[4,5-b] pyridine (PhIP) in CHO cells expressing murine cytochrome P450
IA2. Mutagenesis 6:253-259.
Tucker, J.D., Wyrobek, A.J., Ashworth, L.K., Christensen, M.L., Burton, G.V., Carrano, A.V.,
and Everson, R.B. (1990). Induction, accumulation, and persistence of sister chromatid
exchanges in women with breast cancer receiving cyclophosphamide, adriamycin, and 5fluorouracil chemotherapy. Cancer Research. 50:4951-4956.
Mayall, B.H., Tucker, J.D., Christensen, M.L., van Vliet, L.J. and Young, I.T. (1990).
Experience with Athena, a semi-automated karyotyping system. Cytometry 11:59-72.
Tucker, J., and Eastmond, D. (1989). Use of an antikinetochore antibody to discriminate between
micronuclei induced by aneuploidogens and clastogens. In: Progress in Clinical and
Biological Research, Vol. 340B, (M. L. Mendelsohn and R. J. Albertini, Eds.) pp. 275284.
Ishizaka, Y., Itoh, F., Tahira, T., Ikeda, I., Sugimura, T., Tucker, J., Fertitta, A., Carrano, A.V.,
and Nagao, M. (1989). Human ret proto-oncogene mapped to chromosome 10q11.2.
Oncogene 4:1519-1521.
Eastmond, D.A. and Tucker, J.D. (1989). Kinetochore localization in micronucleated
cytokinesis-blocked Chinese hamster ovary cells: A new and rapid assay for identifying
aneuploidy-inducing agents. Mutation Research 224:517-525.
Tucker, J.D., Taylor, R.T., Christensen, M.L., Strout, C.L., Hanna, M.L., and Carrano, A.V.
(1989). Cytogenetic response to 1,2-dicarbonyls and hydrogen peroxide in Chinese
hamster ovary AUXB1 cells and human peripheral lymphocytes. Mutation Research
224:269-279.
MacGregor, J.T., Tucker, J.D., Ziderman, I.I., Wehr, C.M., Wilson, R.E., and Friedman, M.
(1989). Non-clastogenicity in mice of fructose/lysine and other sugar/amino acid
browning products with in vitro genotoxicity. Food and Chem. Toxicol. 11:715-721.
Turteltaub, K.W., Knize, M.G., Healy, S.K., Tucker, J.D., and Felton, J.S. (1989). The metabolic
disposition of 2-amino-1-methyl-6-phenylimidazo[4,5- b]pyridine (PhIP) in the mouse. J.
of Food and Chemical Toxicology 27:667-673.
Mohrenweiser, H.W., Carrano, A.V., Fertitta, A., Perry, B., Thompson, L.H., Tucker, J.D., and
Weber, C.A. (1989). Refined mapping of the three DNA repair genes, ERCC1, ERCC2,
and XRCC1, on human chromosome 19. Cytogenetics and Cell Genetics 52:11-14.
Felton, J.S., Knize, M.G., Shen, N.H., Wu, R., Taylor, R.T., Fultz, E., Turteltaub, K.W.,
Vanderlaan, M., Hwang, M., Watkins, B.E., Mazrimas, J., Tucker, J.D. (1989).
Identification, metabolism, genotoxicity and modeling of mutagens produced in food by
heating. Var Foda 42:61-64.
Eastmond, D.A., Tucker, J.D., and Pinkel, D. (1989). Use of an antikinetochore antibody and
DNA probes to measure aneuploidy induction in interphase human lymphocytes and
Chinese hamster ovary cells. In: Proceedings of Hanford Life Science Symposium,
Multi-Level Health Effects Research: From Molecules to Man. J.F. Park and R.A. Pelroy,
(Eds.), pp. 157-165.
Tucker, J.D., Taylor, R.T., Christensen, M.L., Strout, C.L., and Hanna, M.L. (1989). Cytogenetic
response to coffee in Chinese hamster ovary AUXB1 cells and human peripheral
lymphocytes. Mutagenesis 4:343-348.
Eastmond, D.A. and Tucker, J.D. (1989). Identification of aneuploidy-inducing agents using
cytokinesis-blocked human lymphocytes and an antikinetochore antibody. Environmental
and Molecular Mutagenesis 13:34-43.
Tucker, J.D., Carrano, A.V., Allen, N.A., Christensen, M.L., Knize, M.G., Strout, C.L., and
Felton, J.S. (1989). In vivo cytogenetic effects of cooked food mutagens. Mutation
Research 224:105-113.
Tucker, J.D., Christensen, M.L., and Carrano, A.V. (1988). Simultaneous identification and
banding of human chromosomal material in somatic cell hybrids. Cytogenetics and Cell
Genetics 48:103-106.
Tucker, J.D., Ashworth, L.K., Johnston, G.R., Allen, N.A., and Carrano, A.V. (1988). Variation
in the human lymphocyte sister chromatid exchange frequency: Results of a long term
longitudinal study. Mutation Research 204:435-444.
Thompson, L.H., Tucker, J.D., Stewart, S.A., Christensen, M.L., Salazar, E.P., Carrano, A.V.,
and Felton, J.S. (1987). Genotoxicity of compounds from cooked beef in repair-deficient
CHO cells versus Salmonella mutagenicity. Mutagenesis, 2:483-487.
Tucker, J.D., Christensen, M.L., Strout, C.L., McGee, K.A., and Carrano, A.V. (1987). Variation
in the human lymphocyte sister chromatid exchange frequency as a function of time:
Results of daily and twice-weekly sampling. Environmental and Molecular Mutagenesis
10:69-78
Tucker, J.D. and Christensen, M. L. (1986). Effects of anticoagulants upon sister chromatid
exchanges, cell cycle kinetics, and mitotic index in human peripheral lymphocytes.
Mutation Research 190:225-228.
Tucker, J.D., Christensen, M.L., Strout, C.L., and Carrano, A.V. (1986). Determination of the
baseline sister chromatid exchange frequency in human and mouse peripheral
lymphocytes using monoclonal antibodies and very low doses of bromodeoxyuridine.
Cytogenetics and Cell Genetics 43:38-42.
Tucker, J.D., Strout, C.L., Christensen, M.L., and Carrano, A.V. (1986). Sister chromatid
exchange induction and persistence in peripheral blood and spleen lymphocytes of mice
treated with ethylnitrosourea. Environmental Mutagenesis 8:345-355.
Tucker, J.D., Xu, J., Stewart, J., Baciu, P.C., and Ong, T. (1986). Detection of sister chromatid
exchanges induced by volatile genotoxicants. Teratogenesis Carcinogenesis and
Mutagenesis 6:15-21.
Sousa, J., Nath, J., Tucker, J.D., and Ong, T. (1985). Dietary factors affecting the mutagenicity
assay system. I. Detection of mutagenic activity in human urine following a fried beef
meal. Mutation Research 149:365-374.
Tucker, J.D. and Ong, T. (1985). Induction of sister chromatid exchanges by coal dust and
tobacco snuff extracts in human peripheral lymphocytes. Environmental Mutagenesis
7:313-324.
Ong, T., Tucker, J.D., Stewart, J.D., and Whong, W-Z. (1984). Development of an in situ system
for detection of mutagens in the workplace. In: Short-Term Genetic Bioassays in the
Evaluation of Complex Mixtures, Waters, MD, Sandhu, S.S., Lewtas, J., Claxton, L.,
Strauss, G., and Nesnow, S. (Eds.).
Baciu, P., Keller, E.C. Jr., and Tucker, J.D. (1984). Association of cancer mortality rates and
exogenous environmental factors in West Virginia. Proc. West Virginia. Acad. Sci.
55:82-91.
Strouse, N., Keller, E.C. Jr., and Tucker, J.D. (1984). Relationships between legal game harvests
and environmental variables in West Virginia. Proc. West Virginia. Acad. Sci. 55:125131.
Tucker, J.D. and Ong, T. (1984). Induction of sister chromatid exchanges and chromosome
aberrations in human peripheral lymphocytes by 2,4,7-trinitro-9-fluorenone. Mutation
Research 138:181-184.
Tucker, J.D., Xu, J., Stewart, J., and Ong, T. (1984). Detection of sister chromatid exchanges in
human peripheral lymphocytes induced by ethylene dibromide vapor. Mutation Research
138:93-98.
Tucker, J.D., Whong, W-Z, Ju, X., and Ong, T. (1984). Genetic activity of nitrosated coal dust
extract in mammalian systems. Environmental Research 35:171-179.
Sorenson, W.G., Tucker, J.D., and Simpson, J.P. (1984). Mutagenicity of the tetramic mycotoxin
cyclopiazonic acid. Applied and Environmental Microbiology 7:1355-1357.