Philip A. Anfinrud
PERSONAL
Address:
Laboratory of Chemical Physics, NIDDK
National Institutes of Health
Bldg. 5 Rm. 134
5 Memorial Drive
Bethesda, MD 20892-0520
Phone & Fax: (301) 435-6034
Fax:
(301) 435-6710
Email:
anfinrud@helix.nih.gov
Web site: http://www.niddk.nih.gov/intram/people/panfinru.htm
EDUCATION
Ph.D.
B.S.
1987, Physical Chemistry, Iowa State University, Picosecond Excited State
Dynamics and Excitation Transport in Solution and on Surfaces
1981, Chemistry (Physics minor), North Dakota State University
PROFESSIONAL EXPERIENCE
1998 –
Senior Biomedical Research Scientist, National Institutes of Health
Summer 1997-Visiting Scientist at the European Synchrotron and Radiation Facility
(ESRF) in Grenoble, FRANCE (collaboration with Dr. Michael Wulff)
1995-1998
Associate Professor of Chemistry, Harvard University, Cambridge, MA
1990-1994
Assistant Professor of Chemistry, Harvard University, Cambridge, MA
1987-1989
Postdoctoral Research Associate with Professor Robin M. Hochstrasser,
University of Pennsylvania, Philadelphia, PA
1982-1987
Research Assistant with Professor Walter S. Struve, Iowa State University
and Ames Laboratory-USDOE, Ames, IA
1981-1982
Teaching Assistant, Iowa State University, Ames, IA
AWARDS
Outstanding Young Alumnus Award, Iowa State University
1997
Beckman Young Investigator Award (BYI)
1992-1994
NSF Young Investigator Award (NYI)
1992-1997
Alpha Chi Sigma Award (outstanding research in Physical Chemistry)
1987
Proctor and Gamble Fellowship
1984
PACE (Premium for ACademic Excellence) Award
1981-1983
Coover Scholarship in Physical Chemistry
1981
COURSES TAUGHT (HARVARD)
Chem. 10
“Foundations in Chemistry,” Fall 1992-1997
Chem. 98hf “Introduction to Research,” Fall 1990
Chem. 165
“Experimental Physical Chemistry,” Spring 1990-1996
Chem. 245
“Molecular Dynamics and Time-Resolved Laser Spectroscopy,” Fall 1991
EXTRAMURAL COMMITTEES
Member of the Advisory Editorial Board for Chemical Physics, 2005APS Scientific Advisory Committee (SAC) charged with a cross-cut review of "Science
Requiring the Pulsed Structure of the APS X-ray Beam,” 2005.
Scientific Program Advisory Committee, Workshop on Time-Domain Science using Xray Techniques, Lake Geneva, Wisconsin, August 2004.
Organizing Committee for Ultrafast Phenomena XIV, Niigata JAPAN, July 2004
Co-chair, Symposium on “Frontiers in Biophysical Methods” 226th ACS National
Meeting, New York, NY, Sept. 2003
DOE BERAC Subcommittee on Biological Applications of Synchrotron Radiation” 2003
Organizing Committee for Ultrafast Phenomena XIII, Vancouver CANADA, May 2002
Expert Committee for “Light Sources”, Canada Foundation for Innovation, March 2002.
Co-chair, Workshop on “Conformational Dynamics in Proteins,” Telluride, CO, 2001
Organizing Committee, German-American Frontiers of Science, 1999
Advisory Committee, International Conference on Oxygen-Binding Heme Proteins, 1998
Program Committee, International Quantum Electronics Conference, 1994
MEMBERSHIPS
American Association for the Advancement of Science
American Chemical Society
Biophysical Society
DEPARTMENT AND UNIVERSITY COMMITTEES
Physical Chemistry Seminar Series
Harvard-MIT Physical Chemistry Seminar Series
Graduate Admissions Committee
Graduate Advisory Committee
Sigma g: Graduate Quality of Life Committee
Shop Committee
Chemistry and Physics Concentration Committee
Publications
“Excitation Transport and Trapping in a Two-Dimensional Disordered System: Cresyl Violet on
Quartz,” Philip A. Anfinrud, Robert L. Crackel, and Walter S. Struve, J. Phys. Chem., 88, 5873,
(1984).
“Optical Shot-Noise-Limited Detection: A Single-Sideband Technique with Flexible Modulation
Frequencies,” Philip A. Anfinrud and Walter S. Struve, Rev. Sci. Instrum., 57, 380, (1986).
“Fluorescence Depolarization of Rhodamine 6G in Glycerol: A Photon-Counting Test of
Three-Dimensional Excitation Transport Theory,” Philip A. Anfinrud, David E. Hart, John F.
Hedstrom, and Walter S. Struve, J. Phys. Chem., 90, 2374, (1986).
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“Excitation Transport and Fluorescence Anisotropy of Rhodamine 3B on Amorphous Quartz,”
Philip A. Anfinrud, David E. Hart, John F. Hedstrom, and Walter S. Struve, J. Phys. Chem., 90,
3116, (1986).
“Picosecond Pump-Probe Experiments on Surface-Adsorbed Dyes: Ground-State Recovery of
Rhodamine 640 on ZnO and Fused Silica,” Philip A. Anfinrud, Timothy P. Causgrove, and
Walter S. Struve, J. Phys. Chem., 90, 5887, (1986).
“Excitation Transport in Solution: A Quantitative Comparison between GAF Theory and
Time-Resolved Fluorescence Profiles,” David E. Hart, Philip A. Anfinrud, and Walter S. Struve,
J. Chem. Phys., 86, 2689, (1987).
“Relationship between Excitation Transport and Fluorescence Depolarization in Two- and
Three-Dimensional Disordered Systems: Effect of Residual Polarization,” Philip A. Anfinrud
and Walter S. Struve, J. Phys. Chem., 91, 5058, (1987).
“Comparison of DODCI Fluorescence Depolarization in Glycerol and Ethylene Glycol: Effect of
Orientational Correlation on Excitation Transport,” Philip A. Anfinrud and Walter S. Struve, J.
Chem. Phys., 87, 4256, (1987).
“Time-Correlated Photon-Counting Probe of Singlet Excitation Transport and Restricted
Rotation in Langmuir-Blodgett Monolayers,” Philip A. Anfinrud, David E. Hart, and Walter S.
Struve, J. Phys. Chem., 92, 4067, (1988).
“Picosecond and Femtosecond Infrared Spectroscopy with CW Diode Lasers,” Philip A.
Anfinrud, Chul-Hee Han, Patricia A. Hansen, John N. Moore, and Robin M. Hochstrasser, in
Ultrafast Phenomena VI, eds. T. Yajima, K. Yoshihara, C.B. Harris, S. Shionoya (Springer,
Berlin), pp. 442-446 (1988).
“Minimization of Discrimination Jitter in Time-Correlated Single Photon Counting,” Philip A.
Anfinrud and Walter S. Struve, Rev. Sci. Instrum., 60, 800, (1989).
“Direct Observations of Ligand Dynamics in Hemoglobin by Subpicosecond Infrared
Spectroscopy,” Philip A. Anfinrud, Chul-Hee Han, Robin M. Hochstrasser, Proc. Natl. Acad.
Sci. USA, 86, 8387, (1989).
“Evolution of the Transient Vibrational Spectrum following Short Pulse Excitation,” Philip A.
Anfinrud, Chul-Hee Han, Tianquan Lian, and Robin M. Hochstrasser, J. Phys. Chem., 94, 1180,
(1990).
“Subpicosecond Infrared Spectroscopy: the Condensed Phase Photochemistry of Iron
Carbonyls,” Philip A. Anfinrud, Chul-Hee Han, Tianquan Lian, and Robin M. Hochstrasser, in
Ultrafast Phenomena VII, eds. C.B. Harris, E.P. Ippen, G.A. Mourou, A.H. Zewail, (Springer,
Berlin), 489-491, (1990).
“Femtosecond Infrared Spectroscopy of Complex Molecules Using CW IR Lasers,” Robin M.
Hochstrasser, Philip A. Anfinrud, Rolf Diller, Chul-Hee Han, Mark Iannone, Tianquan Lian, and
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Bruce Locke, in Ultrafast Phenomena VII, eds. C.B. Harris, E.P. Ippen, G.A. Mourou, A.H.
Zewail, (Springer, Berlin), 429-433, (1990).
“Femtosecond Infrared Spectroscopy: The Ultrafast Photochemistry of Iron Carbonyls in
Solution,” Philip A. Anfinrud, Chul-Hee Han, Tianquan Lian, and Robin M. Hochstrasser, J.
Phys. Chem., 95, 574, (1991).
“Ultrafast Laser Techniques in Spectroscopy,” Philip A. Anfinrud, Carey K. Johnson, Roseanne
J. Sension, and Robin M. Hochstrasser, in APPLIED LASER SPECTROSCOPY: Techniques,
Instrumentation, and Applications, VCH Publishers, Inc., (1993).
“Ultrafast Near-IR Spectroscopy of Carbonmonoxymyoglobin: The Dynamics of Protein
Relaxation,” Manho Lim, Timothy A. Jackson, and Philip A. Anfinrud, Springer Series in
Chemical Physics, 55, pp. 522-524 (1993).
“Nonexponential Protein Relaxation: Dynamics of Conformational Change in Myoglobin,”
Manho Lim, Timothy A. Jackson, and Philip A. Anfinrud, Proc. Natl. Acad. Sci. USA., 90, 58014 (1993).
“Dynamics of Conformational Relaxation in Myoglobin,” Manho Lim, Timothy A. Jackson, and
Philip A. Anfinrud, in Proc. SPIE-Int. Soc. Opt. Eng., 1921, pp. 221-30 (1993).
“Complex Non-Exponential Relaxation in Myoglobin after Photodissociation of MbCO:
Measurement and Analysis from 2 ps to 56 s,” Timothy A. Jackson, Manho Lim, and Philip A.
Anfinrud, Chemical Physics, 180, 131 (1994).
“Femtosecond IR Spectroscopy: Methods and Applications to Protein Dynamics,” Philip A.
Anfinrud, Manho Lim, and Timothy A. Jackson, SPIE-Int Soc. Opt. Eng. (Longer Wavelength
Lasers and Applications), 2138, 107 (1994).
“Ultrafast Mid-IR Spectroscopy of Carbonmonoxymyoglobin: The Dynamics of Ligand Motion,”
Manho Lim, Timothy A. Jackson, and Philip A. Anfinrud, Springer Series in Chemical Physics,
60, pp. 443-4 (1994).
“Mid-IR Vibrational Spectrum of CO after Photodissociation from Heme: Evidence for a Ligand
Docking Site in the Heme Pocket of Hemoglobin and Myoglobin,” Manho Lim, Timothy A.
Jackson, and Philip A. Anfinrud, J. Chem. Phys., 102, 4355 (1995).
“Probing the Dynamics of Ligand Motion in Myoglobin with Femtosecond IR Spectroscopy,”
Timothy A. Jackson, Manho Lim, and Philip A. Anfinrud, Proceedings of the Seventh
International Conference on Time-Resolved Vibrational Spectroscopy, Sante Fe, NM, pp. 9-13
(1995).
“Binding of CO to Myoglobin from a Heme Pocket Docking Site to Form Nearly Linear
FeCO,” Manho Lim, Timothy A. Jackson, and Philip A. Anfinrud, Science, 269, 962 (1995).
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“Femtosecond Near-IR Absorbance Study of Photoexcited Myoglobin: The Dynamics of
Electronic and Thermal Relaxation,” Manho Lim, Timothy A. Jackson, and Philip A. Anfinrud,
J. Phys. Chem., 100, 12043 (1996).
“Ultrafast Photoisomerization of Retinal in Bacteriorhodopsin: A New Twist,” Feng Gai, Kenton
C. Hasson, and Philip A. Anfinrud, Ultrafast Phenomena X, Springer Series in Chemical
Physics, 62, 353 (1996).
“The Photoisomerization of Retinal in Bacteriorhodopsin: Experimental Evidence for a ThreeState Model,” Kenton C. Hasson, Feng Gai, and Philip A. Anfinrud, Proc. Natl. Acad. Sci. USA,
93, 15124 (1996).
“Ultrafast Rotation and Trapping of Carbon Monoxide Dissociated from Myoglobin,” Manho
Lim, Timothy A. Jackson, and Philip A. Anfinrud, Nature Structural Biology, 4 (3), 209 (1997).
“Pump-Dump-Probe Spectroscopy of Bacteriorhodopsin: Evidence for a Near-IR Excited State
Absorbance,” Feng Gai, J. Cooper McDonald, and Philip A. Anfinrud, J. Am. Chem. Soc., 119,
6201 (1997).
“Modulating Carbon Monoxide Binding Affinity and Kinetics in Myoglobin: the Roles of the
Distal Histidine and the Heme Pocket Docking Site,” Manho Lim, Timothy A. Jackson, and
Philip A. Anfinrud, J. Biol. Inorg. Chem, 2, 531 (1997).
“Time-Resolved IR Spectroscopy: Methods and Applications,” Rebecca M. Slayton and Philip
A. Anfinrud, Current Opinion in Structural Biology, 7,717 (1997).
“Femtosecond Time-Resolved Spectroscopy of the Primary Photochemistry of Phytochrome,”
Frank Andel III, K.C. Hasson, Feng Gai, J. Clark Lagarias, Philip A. Anfinrud, and Richard A.
Mathies, Biospectroscopy, 3, 421 (1997).
“Chemical Dynamics in Proteins: The Photoisomerization of Retinal in Bacteriorhodopsin,”
Feng Gai, K.C. Hasson, J.C. McDonald, and Philip A. Anfinrud, Science 279, 1886 (1998).
“Femtochemistry,” Y. Tanimura, K. Yamashita, and P. A. Anfinrud, Proc. Natl. Acad. Sci., USA,
96, 8823 (1999).
“Ultrafast detection and control of molecular dynamics,” P. Anfinrud, R. de Vivie-Riedle, and V.
Engel, Proc. Natl. Acad. Sci., USA, 96, 8328 (1999).
“Vibrational population relaxation of carbon monoxide in the heme pocket of photolyzed
carbonmonoxy myoglobin: Comparison of time-resolved mid-IR absorbance experiments and
molecular dynamics simulations,” D. E. Sagnella, J. E. Straub, T. A. Jackson, M. Lim, and P. A.
Anfinrud, Proc. Natl. Acad. Sci., USA, 96, 14324 (1999).
“Ultrafast time-resolved studies of ligand dynamics ln Myoglobin: Watching proteins as they
function,” P. A. Anfinrud, Abstracts 219: 1009-CHED , Part 1 MAR 26 2000.
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“Time-resolved IR studies of ligand dynamics in heme proteins,” M. Lim, T.A. Jackson, and P.A.
Anfinrud, book chapter in Ultrafast Infrared and Raman Spectroscopy, Marcel Dekker, Inc.,
2001.
“Ultrafast photoinduced dynamics in protein crystals: Examining the influence of the crystal
lattice,” PA Anfinrud, JA Roberts, AV Smirnov, Biophysical Journal 80, 1713 (2001).
“Picosecond Structural Studies using Pulsed Synchrotron Radiation,” F. Schotte, S. Techert, P.A.
Anfinrud, V. Srajer, K. Moffat, and M. Wulff, book chapter in Third-Generation Hard X-ray
Synchrotron Radiation Sources: Source Properties, Optics, and Experimental Techniques,
Wiley, New York, 2002.
“Watching a protein as it functions with 150 ps time-resolved X-ray crystallography,” F. Schotte,
M. Lim, T.A. Jackson, A.V. Smirnov, J. Soman, J.S. Olson, G.N. Phillips, Jr., M. Wulff, and
P.A. Anfinrud, Science, 300, 1944 (2003).
“Complex landscape of protein structural dynamics unveiled by nanosecond Laue
crystallography,” D. Bourgeois, B. Vallone, F. Schotte, A. Arcovito, A. E. Miele, G. Sciara, M.
Wulff, P.Anfinrud, and M. Brunori, Proc. Natl. Acad. Sci. USA, 100, 8704 (2003).
“The orientational distribution of CO before and after photolysis of MbCO and HbCO: A
determination using time-resolved polarized mid-IR spectroscopy,” M. Lim, T.A. Jackson, and
P.A. Anfinrud, Journal of the American Chemical Society, 126, 7946-7957 (2004).
“Visualizing Chemical Reactions in Solution by Picosecond X-Ray Diffraction,” Anton Plech,
Michael Wulff, Savo Bratos, Fabien Mirloup, Rodolphe Vuilleumier, Friedrich Schotte, and
Philip A. Anfinrud, PRL 92, 125505 (2004).
“Chromophore Conformation and the Evolution of Tertiary Structural Changes in Photoactive
Yellow Protein,” Spencer Anderson, Vukica Srajer, Reinhard Pahl, Sudarshan Rajagopal,
Friedrich Schotte, Philip Anfinrud, Michael Wulff, and Keith Moffat, Structure 12, 1039 (2004).
“Picosecond Time-Resolved X-ray Crystallography: Probing Protein Function in Real Time,”
Friedrich Schotte, Jayashree Soman, John S. Olson, Michael Wulff, and Philip A. Anfinrud,
Journal of Structural Biology 147/3, 235 (2004).
“Watching proteins function with picosecond time-resolved X-ray crystallography,” Philip
Anfinrud, Friedrich Schotte, and Michael Wulff, in Ultrafast Phenomena XIV, Springer Series in
Chemical Physics, 79, 581 (2004).
“Unveiling functional protein motions with picosecond X-ray crystallography and molecular
dynamics simulations,” Gerhard Hummer, Friedrich Schotte, and Philip A. Anfinrud, Proc. Natl.
Acad. Sci. USA, 101, 15330 (2004).
“Ultrafast Time-resolved IR studies of Protein-Ligand Interactions,” Manho Lim and Philip A.
Anfinrud, Book Chapter in “Methods in Molecular Biology”, Ed. U. Nienhaus, Humana Press
Inc., 305, 243 (2005).
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“Visualizing reaction pathways in photoactive yellow protein from nanoseconds to seconds,”
Ihee H, Rajagopal S, Srajer V, Pahl R, Anderson S, Schmidt M, Schotte F, Anfinrud PA, Wulff
M, Moffat K., Proc. Natl. Acad. Sci., USA, 102, 7145 (2005).
“X-ray fingerprinting of chemical intermediates in solution,” Philip A. Anfinrud and Friedrich
Schotte, Science, XX, XXX (2005).
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