Quantitative Determination of Fluoxetine in Pharmaceutical Formulations by Reversed-Phase Thin-Layer Chromatography Ibrahim. A. Amara, T. Gondovab, Abubaker. Sharifa a Department of Chemistry, Faculty of Sciences, Sebha University, Sebha Libya E-mail; [email protected] b Department of physical and Analytical Chemistry, Faculty of Sciences,P.J.Safarik, University Moyzesova 11, SK- 040 01 Kosice, Slovak Republic A simple, rapid, selective and precise PR-TLC densitometric method has been established for determination of fluoxetine (FLX) in pharmaceutical formulations. The method employed RP 18-TLC plates with methanol-0.05 M phosphate buffer pH 5triethylamine (68 : 27 : 5, v/v/v) as mobile phase. Densitometric analysis was performed in the absorbance mode at λ = 230 nm. The analytical method has excellent linearity (r = 0.9993) in the working concentration rang 100-1000 μg ml-1. The limits of detection and quantitation were found to be 163.5 and 545.7 ng per spot respectively. The recoveries of fuoxetine in pharmaceutical formulations were 97.48 and 106.75 %. Statistical analysis proved that the method was precise and accurate for estimation of the drug.