1. Female: AO, since father had a B phenotype, he must have contributed an i allele. Male: AO, since mother must have donated an i allele. Possible genotypes, therefore would be A or O. Ans: two of the above are correct (e) 2. Dominance Relationships: C>ck>cd>ca Sepia x cream: since the sepia parent had an albino parent (caca) its genotype must be ckca. The cream individual had two sepia parents- one of those parents could be either ckcd OR ckca. One cross would therefore be: ckca x cdcd ½ cd ½ ck ½ ckcd (sepia) ½ cd ½ cd ½ ca ½ cacd (cream) ½ cd Ans: 1 sepia: 1 cream (a) The other cross would therefore be: ckca x cdca ½ cd ½ ck 2/4 ckcd (sepia) ½ ca ½ cd 1/4 cacd (cream) ½ ca ½ ca 1/4 caca (albino) Ans: 2 sepia: 1 cream: 1albino I will count both (a) and (e) on personal quizzes. 3. Sepia x cream: since the sepia parent had two full color parents (Cck x Cck,d,a) its genotype could be ckck,d, or a. Again, the cream individual had two sepia parents- one of those parents could be either ckcd OR ckca. The results could be the same as 2, and in addition, all sepia (if first parent was ckck ) I will count both (a) and (e) on personal quizzes. 4. These results are a variation on what would be expected with a monohybrid F1 cross, or a testcross. Since palomino does not breed true, it does not seem to be due to a specific allele, and cross #3 argues for incomplete dominance: Chestnut x cDcD = chestnut Chestnut x cdcd = cremelo Chestnut x cDcd = palomino cDcd x cDcd = ¼ cDcD: ½ cDcd: ¼ cdcd Ans: incomplete dominance (b) 5. Cross number 3 suggests that this cross is an example of 4 distinct phenotypes arising from a dihybrid cross, since it is a variant 9:3:3:1 ratio. The gray individuals would be homozygous at one locus (AAbb) the brown individuals would be homozygous at a second locus (aaBB) and individuals which inherit at least one dominant allele at both loci would be green (A_B_). Individuals that are homozygous recessive at both alleles (aabb) would be blue. Since the F2 ratio is a variant 9:3:3:1 ratio, the F1 must be heterozygotes (AaBb). Since one of the cats was gray (e.g. AAbb), the other parent must have been brown (aaBB). Ans: AAbb x aaBB; (c) 6. The parental classes would be the most abundant: (pr + +) and (+ v bm) The double recombinant classes would be the least abundant: (+ + +) and (pr v bm) Therefore, the middle allele must be purple: + pr + v + bm Ans: pr (a) 7. v-pr interval: v pr + + + bm +++ v pr bm 79 82 42 44 247 247/1109 = 0.223 22.3 map units Phenotype Number of Progeny virescent 200 purple 237 brown 82 virescent, brown 230 virescent, purple 79 purple, brown 195 wild type 42 virescent, purple, brown 44 pr-bm interval: + pr bm 195 v++ 200 +++ 42 v pr bm 44 481 481/1109 = 0.434 43.4 map units Ans: 43.4 map units (c) v- bm interval: 22.3 + 43.4 = 65.7 map units. 8. Observed DCOs: 42+44 = 86 Expected DCOs: (0.434*0.223*1109) = 107.3 86/107 ≈ 0.803 = coefficient of coincidence 1-0.803 = 0.196 Ans: (b) Spore number 1 2 3 4 5 6 7 8 totals type segregation -------------------------------------------------Ascus Types------------------------------------------ 1 2 3 4 5 6 7 ++ ++ ab ab +b +b a+ a+ 5 TT 2:2 ++ ++ ab ab ++ ++ ab ab 1 NPD 2:2 +b +b ab ab ++ ++ a+ a+ 5 TT 2:1 a+ a+ a+ a+ +b +b +b +b 808 PD 1:1 9. 5+1+5+90= 101/1000* ½ = 0.0505 = 5.05 map units Ans: (a) 10. RF between a and b = (½ TT+ NPD)/total ½ (5 + 5 + 90) + 2 = 52 52/1000 = 0.052 = 5.2 map units Ans: (b) +b +b a+ a+ +b +b a+ a+ 90 PD 2:2 ++ ++ ++ ++ ab ab ab ab 1 NPD 1:1 ++ ++ +b +b a+ a+ ab ab 90 TT 1:2