Answers to Quiz #2: 1. In birds, the female is the heterogametic sex (ZW), and the male is the homogametic sex (ZZ). In this cross, there are two allelic variants on the Z chromosome that govern feather color: G = green, is dominant; g = yellow, is recessive. ZGW ♀ x ZgZg♂ Green females x yellow males ↓ ZgZG♂ and ZgW ♀ Green males and yellow females ↓ g g F2 males would be ½ Z Z (yellow) and ½ ZgZG (green) Ans: all of the above (d). 2. C (full color) >ck (sepia) > cd (cream) > ca (albino). If both of the parentals had an albino parent, their genotypes must be: ck ca ckca x cdca sepia x cream ↓ d c ca d k a k c c (sepia) c c (sepia) cdca (cream) caca (albino) Ans: 2 sepia: 1 cream: 1 albino (e) none of the above. Cross Parental F1 F2 1 Green x gray Green ¾ green: ¼ gray 2 Green x brown Green ¾ green: ¼ brown 3 Gray x brown Green 9/16 green: 3/16 brown 3/16: gray: 1/16 blue The last cross here shows a 9:3:3:1 relationship, indicative of a dihybrid cross situationtwo separate gene loci are involved. This would imply that at least one dominant allele is required for green (A_B_) and the blue phenotype is all homozygous recessive (aabb). Gray and Brown phenotypes would represent situations where only one locus had a dominant allele and the other was homozygous recessive. (e.g. A_bb = gray; aaB_ = brown). Since cross #3 is with true-breeding strains, AAbb x aaBB → AaBb x AaBb 3. Ans: false. 4. AABB x aabb would produce green individuals who were homozygous recessive for both loci, the same as cross # 3. Ans: (c) the same results as cross #3. 5. The male parent (AO) must have inherited an O allele from his mother: otherwise, the male would be AB. The same logic holds for the female parent (BO); she must have inherited an O allele from her father. The cross is therefore: AO x BO→ 1 AO: 1BO: 1AB: 1OO; Ans: (e) all classes would be expected. Phenotype Number of progeny Abc 18 aBc 630 abc 148 AB c 132 a BC 22 abC 120 ABC 140 AbC 615 total 1825 Phasing relationships in heterozygous parent: a c B A C b a-c interval a+b 120 +c+ 132 a++ 22 +cb 18 Total 292/1825 = 0.16 c-b interval acb 148 +++ 140 a++ 22 +cb 18 Total 338/1825 = 0.1797 6. Ans: 16 map units (c) 7. (0.16)(0.1797)1825 = 52.48 COC = 40/52.48 = 0.762 Ans: (c) Phenotype Number of progeny Looking at this data set, we see distributions that are significantly different than in the data set above; abC 228 in this example, four classes are found in high aBc 235 frequency and 4 are found in low frequency. This aBC 16 suggests that one gene locus may be showing abc 22 independent assortment, i.e. is not linked to the AbC 224 other two. To determine this, look at how each of ABc 233 the two genes segregate relative to one another: ABC 22 Abc 20 total 1000 AB = 233 + 22 BC = 16 + 22 Ab = 224 + 20 Bc = 235 + 233 aB = 235 + 16 bC = 228 + 224 ab = 228 + 22 bc = 22 + 20 The A and B gene loci are assorting independently- all gametes are found in about equal frequencies; the B and C gene loci are linked- the nonrecombinant chromosomes have genes in the trans configuration : Bc/bC are the nonrecombinants, bc/BC are the recombinants. Recombination between the B and C gene loci here are 80/1000 = 0.08 or 8 map units. Ans: (d) all of the above Spore pairs total 1 Ab Ab aB aB 127 2 AB AB ab ab 125 3 Ab AB ab aB 100 4 Ab ab AB aB 36 5 Ab aB Ab aB 2 6 AB ab AB ab 4 7 AB ab Ab aB 6 9. To measure the distance between a gene and a centromere, identify number of asci showing a second division segregation pattern… 36 + 2 + 4 + 6 = 48/400 (1/2) = 0.06 Ans: (a) 6 map units 10. Are the two genes linked? If the genes are linked, then PD >> NPD PD = 127 + 2 = 129 NPD = 125 + 4 = 129 The genes are NOT linked… Ans: (e)