Course in Proteome
Separation, Analysis and Quantification
from 5 to 9 February 2007
at the Turku Centre for Biotechnology
Organized by Plant Biology and Biological Interaction graduate schools,
and the CBT VTT Proteomics Facility
The five-day training course will include both hands-on experience and lectures on basic
proteomics techniques. The aim of the training course is to provide an overview of
proteomics methods covering sample preparation, protein and peptide separations, and mass
spectrometric analyses. Tutorial sessions will cover the theory of the techniques used in the
course. Lectures will cover proteomics applications and theory about mass spectrometry in
proteomics. This course is useful for those who want to learn more about gel based
separation techniques, protein identification by mass spectrometry or quantitative proteomics
analyses.
The course will cover the following topics
 Sample preparation, for identification and
quantitative analysis
 Purification of single proteins, complex mixtures
and protein complexes
 Principles of MS methods
 Protein identification via MS
 Protein phosphorylation analysis via MS
 Quantitative analysis of proteins
Techniques
 One and two-dimensional electrophoresis
 Peptide isoelectric focusing (IEF)
 Blue Native gel electrophoresis
 Capillary liquid chromatography
 Quantitative analysis via iTRAQ chemistry
 MALDI-TOF MS & MALDI-TOF/TOF MS/MS
 LC/ESI-MS/MS
 Data analysis and interrogation
Literature task
 Publications on state-of-the-art proteomics applications and technologies will be sent to all participants
beforehand. During the course participants (in groups of four) will prepare a ‘journal club’ overview for
presentation.
Course Details
Location
Duration
Maximum participants
Organisation
Program coordination
Course fee
Applications
Registration deadline
More information
CBT VTT Proteomics Facility, Turku Centre for Biotechnology, Tykistökatu 6, BioCity 5th floor,
20520 Turku, Finland
5 days
16 (8 from each graduate school)
Eva-Mari Aro, Plant Biology graduate school, Turku, evaaro@utu.fi
Garry Corthals, CBT VTT Proteomics Facility, Turku, gcorthal@btk.fi
Questions about course material
Anne Rokka, CBT VTT Proteomics Facility, Turku, arokka@btk.fi
Natalia Battchikova, Plant Phys & Mol Biol. lab.,. Turku, natbat@utu.fi
Priority for PB and BI graduate schools, no course fee. Others considered afterwards
By email to Eva-Mari Aro evaaro@utu.fi
20th January 2007
http://www.sci.utu.fi/biovuo/index.html
http://www.helsinki.fi/bioscience/fgspb