Nicotine Sulfate CAS No.65-30-5

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UCSF Lab Standard Operating Procedure
Chemical Process, Name or Hazard Class:
Nicotine Sulfate
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☒Hazardous Chemical
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Purpose
Nicotine Sulfate is an acute toxin. This SOP provides information about its hazards and how to mitigate them
through proper controls, handling, and storage.
Physical & Chemical Properties/Definition of Chemical Group
CAS#: 65-30-5
Class: Acute Toxicant
Molecular Formula: C10H14N2
Form (physical state): Oily liquid
Color: Pale yellow turning brown upon exposure to air
Boiling point: 477 °F
Potential Hazards/Toxicity
Oral LD50 [Rat]: 1.6 mg/kg
Nicotine Sulfate
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Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/)
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Inhalation: Inhalation of aerosols (mists, fumes), generated by the material during the course of
normal handling, may produce severely toxic effects. Relatively small amounts absorbed from the
lungs may prove fatal.
Ingestion: Toxic effects may result from the accidental ingestion of the material; animal experiments
indicate that ingestion of less than 40 grams may be fatal or may produce serious damage to the
health of the individual. Nicotine Sulfate is amongst the most toxic of poisons, acting quickly. The
probable lethal dose by ingestion, in man, is about 0.5-1.0 mg/kg. Symptoms of poisoning may
develop within 15 minutes.
Skin Contact: May produce severely toxic effects; systemic effects may result following absorption
and these may be fatal.
Eye Contact: There is some evidence to suggest that this material can cause eye irritation and damage
in some persons. The vapor may produce pronounced discomfort of the eyes when present at higher
concentrations and this generally gives warning of excessive exposure and the need for control
measures to ensure safe working conditions.
Chronic Exposure: May cause cancer.
Engineering Controls
Work with this chemical in a certified ducted fume hood. Facilities storing or utilizing this material should be
equipped with an eyewash facility and a safety shower.
Personal Protective Equipment (PPE)
Respirator Protection
If lab personnel would like to use respirator on a voluntary basis, they must be trained and fit-tested by
EH&S. This is a regulatory requirement. (http://www.ehs.ucsf.edu/respiratory-protection-program)
Hand Protection
Handle with PVC gloves. Gloves must be inspected prior to use. Use proper glove removal technique (without
touching glove's outer surface) to avoid skin contact with this product. Dispose of contaminated gloves after
use in accordance with applicable laws and good laboratory practices. Wash and dry hands.
NOTE: Consult with your preferred glove manufacturer to ensure that the gloves you plan on using are
compatible with Nicotine Sulfate.
Refer to glove selection chart from the links below:
http://www.ansellpro.com/download/Ansell_8thEditionChemicalResistanceGuide.pdf
OR
http://www.allsafetyproducts.biz/page/74172
OR
http://www.showabestglove.com/site/default.aspx
OR
http://www.mapaglove.com/
Eye Protection
ANSI approved safety glasses.
Skin and Body Protection
Lab coats should be worn. These laboratory coats must be appropriately sized for the individual and be
buttoned to their full length. Laboratory coat sleeves must be of a sufficient length to prevent skin exposure
Nicotine Sulfate
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Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/)
while wearing gloves. Full length pants and close-toed shoes must be worn at all times by all individuals that
are occupying the laboratory area. The area of skin between the shoe and ankle should not be exposed.
Hygiene Measures
Avoid contact with skin, eyes and clothing. Wash hands before breaks and immediately after handling the
product.
First Aid Procedures
If inhaled
Remove to fresh air. If not breathing, give artificial respiration. If breathing is difficult, give oxygen. Get
medical attention.
In case of skin contact
Immediately flush skin with plenty of water for at least 15 minutes while removing contaminated clothing
and shoes. If dilute vinegar solutions are available apply these IMMEDIATELY to any area of skin which has
been affected. Get medical attention immediately. Wash clothing before reuse. Thoroughly clean shoes before
reuse. Contaminated work clothes should be laundered by individuals who have been informed of the
hazards of exposure to this substance.
In case of eye contact
Immediately flush eyes with plenty of water for at least 15 minutes, lifting lower and upper eyelids
occasionally. Get medical attention.
If swallowed
Never give anything by mouth to an unconscious person. Rinse mouth with water. Get medical attention.
Notes to physician
For Nicotine Sulfate intoxication:
Administer 6 to 8 heaped teaspoons of activated charcoal, as a slurry in water. Because Nicotine Sulfate
induces vomiting by stimulating the chemoreceptor trigger zones of the brainstem, it seems inadvisable to
administer syrup of Ipecac, which acts by the same mechanism.
Unless spontaneous emesis is vigorous and productive, gastric lavage with a 0.5% solution of tannic acid, or a
1:5000 solution of potassium permanganate. Use water if these solutions are not immediately available.
If Nicotine Sulfate is spilled on skin, wash thoroughly and IMMEDIATELY with diluted vinegar and / or cold
running water. (Nicotine Sulfate salts are less readily absorbed).
Administer artificial ventilation and oxygen therapy until spontaneous breathing is adequate or until the
heart ceases to beat. Central respiratory stimulants are rarely if ever indicated. Keep airway clear. Profuse
salivation may require continuous oral suction.
If severe or persistent, convulsions may be controlled with small intravenous doses of barbiturates or
diazepam.
Most of the visceral manifestations can be controlled by various combinations of autonomic blocking drugs,
such as atropine and phenoxybenzamine (Dibenzyline). Caramiphen (Parpanit) hydrochloride and diethazine
(Diparcol) hydrochloride have been recommended in the experimental poisoning but may not be readily
available.
GOSSELIN, SMITH & HODGE: Clinical Toxicology of Commercial Products, 5th Ed.
Nicotine Sulfate undergoes a large first-pass effect during which the liver metabolises 80-89%. Smaller
amounts are metabolised in the lung and kidney. Nicotine Sulfate and its metabolites (cotinine and Nicotine
Sulfate-1'-N-oxide) are excreted in the urine. At a pH of 5.5 or less, 23% is excreted unchanged. At a pH of 8,
Nicotine Sulfate
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Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/)
only 2% is excreted in the urine. The effect of urinary pH on total clearance is entirely due to changes in renal
clearance. Blood cotinine levels, and possibly, saliva cotinine levels, are good measures of passive smoking.
Tobacco is less toxic than expected from its Nicotine Sulfate content, among tobacco chewers. Apparently
intestinal absorption of Nicotine Sulfate from tobacco is so slow that metabolic inactivation keeps pace with
absorption.
Special Handling and Storage Requirements
Handling: Wear personal protective equipment. Ensure adequate ventilation. Do not get in eyes, on skin, or
on clothing. Do not ingest. Keep away from clothing and other combustible materials.
Conditions for safe storage: Store in secondary containment with Acute Toxin label on the primary
container, secondary containment and the storage location. Keep containers tightly closed in a dry, cool, and
well-ventilated place. Avoid strong oxidizing agents.
Spill and Accident Procedure
Chemical Spill Dial 9-911 from campus phone or 415-476-1414 from cell phone or 415-2068522 (SFGH only)
Spill – Assess the extent of danger. Assist contaminated or injured persons. Evacuate the spill area.
Avoid breathing vapors. If possible, confine the spill to a small area using a spill kit or absorbent
material. Keep others from entering contaminated area (e.g., use caution tape, barriers, etc.).
Small (<1 L) – If you have training, you may assist in the clean-up effort. Use appropriate personal
protective equipment and clean-up material for chemical spilled. Double bag spill waste in clear
plastic bags, label and take to the next chemical waste pick-up.
Large (>1 L) – Dial 9-911 from campus phone or 415-476-1414 from cell phone or 415-2068522 (SFGH only) for assistance.
Chemical Spill on Body or Clothes – Remove clothing and rinse body thoroughly in emergency
shower for at least 15 minutes. If discomfort persists, proceed to the Emergency Department. If no
further discomfort is experienced, have the SDS ready and contact Poison Control Hotline at 1-800222-1222 for further exposure information. Notify your direct supervisor and EH&S at 415-4761300 during work hours, or 9-911 during non-working hours and weekends.
Chemical Splash Into Eyes – Immediately rinse eyeball and inner surface of eyelid with water for
15 minutes by forcibly holding the eye open. If discomfort persists, proceed to the Emergency
Department. If no further discomfort is experienced, have the SDS ready and contact Poison
Control Hotline at 1-800-222-1222 for further exposure information. Notify your direct
supervisor and EH&S at 415-476-1300 during work hours, or 9-911 during non-working
hours and weekends.
Medical Emergency Dial 9-911 (campus phone) or 476-6911 (cell phone)
Nicotine Sulfate
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Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/)
Note: All serious injuries must be reported to EH&S at 415-476-1300 within 8 hours.
Non-Life Threatening Emergency– Go to Occupational Health Programs (OHP) Clinic, 415-8857580, 2330 Post Street, Suite 460 Hours of Operation for Appointments: Monday - Friday
7:30 a.m. - 4:00 p.m. (except Holidays).
Note: All serious injuries must be reported to EH&S at 415-476-1300 within 8 hours.
Needle stick/puncture exposure (as applicable to chemical handling procedure) – Wash the
affected area with antiseptic soap and warm water for 15 minutes. For mucous membrane
exposure, flush the affected area for 15 minutes using an eyewash station. Page the needle stick
nurse by dialing 415-353-7842 (STIC).
Decontamination/Waste Disposal Procedure
Clean contaminated surfaces with soap and water and paper towels. Dispose of the paper towels as
hazardous waste.
Safety Data Sheet (SDS) Location
Online SDS can be accessed at http://www.ehs.ucsf.edu/safety-data-sheet-sds-1
Protocol/Procedure
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General Overview and Purpose:
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NOTE
Any deviation from this SOP requires approval from the Principal Investigator.
Nicotine Sulfate
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Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/)
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