UCSF Lab Standard Operating Procedure Chemical Process, Name or Hazard Class: Nicotine Sulfate Please fill out the form completely. Insure all users have access to this SOP. Refer to instructions for assistance. Enter text. Department: Date SOP was written: Enter date. This lab specific SOP has been reviewed and approved by: Principal Investigator Name: Enter text. Principal Investigator Signature Lab Manager/Supervisor: Type of SOP: ☐ Process Enter text. ☒Hazardous Chemical ☐ Hazardous Class Purpose Nicotine Sulfate is an acute toxin. This SOP provides information about its hazards and how to mitigate them through proper controls, handling, and storage. Physical & Chemical Properties/Definition of Chemical Group CAS#: 65-30-5 Class: Acute Toxicant Molecular Formula: C10H14N2 Form (physical state): Oily liquid Color: Pale yellow turning brown upon exposure to air Boiling point: 477 °F Potential Hazards/Toxicity Oral LD50 [Rat]: 1.6 mg/kg Nicotine Sulfate 1 Date: Click here to enter a date. Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/) Inhalation: Inhalation of aerosols (mists, fumes), generated by the material during the course of normal handling, may produce severely toxic effects. Relatively small amounts absorbed from the lungs may prove fatal. Ingestion: Toxic effects may result from the accidental ingestion of the material; animal experiments indicate that ingestion of less than 40 grams may be fatal or may produce serious damage to the health of the individual. Nicotine Sulfate is amongst the most toxic of poisons, acting quickly. The probable lethal dose by ingestion, in man, is about 0.5-1.0 mg/kg. Symptoms of poisoning may develop within 15 minutes. Skin Contact: May produce severely toxic effects; systemic effects may result following absorption and these may be fatal. Eye Contact: There is some evidence to suggest that this material can cause eye irritation and damage in some persons. The vapor may produce pronounced discomfort of the eyes when present at higher concentrations and this generally gives warning of excessive exposure and the need for control measures to ensure safe working conditions. Chronic Exposure: May cause cancer. Engineering Controls Work with this chemical in a certified ducted fume hood. Facilities storing or utilizing this material should be equipped with an eyewash facility and a safety shower. Personal Protective Equipment (PPE) Respirator Protection If lab personnel would like to use respirator on a voluntary basis, they must be trained and fit-tested by EH&S. This is a regulatory requirement. (http://www.ehs.ucsf.edu/respiratory-protection-program) Hand Protection Handle with PVC gloves. Gloves must be inspected prior to use. Use proper glove removal technique (without touching glove's outer surface) to avoid skin contact with this product. Dispose of contaminated gloves after use in accordance with applicable laws and good laboratory practices. Wash and dry hands. NOTE: Consult with your preferred glove manufacturer to ensure that the gloves you plan on using are compatible with Nicotine Sulfate. Refer to glove selection chart from the links below: http://www.ansellpro.com/download/Ansell_8thEditionChemicalResistanceGuide.pdf OR http://www.allsafetyproducts.biz/page/74172 OR http://www.showabestglove.com/site/default.aspx OR http://www.mapaglove.com/ Eye Protection ANSI approved safety glasses. Skin and Body Protection Lab coats should be worn. These laboratory coats must be appropriately sized for the individual and be buttoned to their full length. Laboratory coat sleeves must be of a sufficient length to prevent skin exposure Nicotine Sulfate 2 Date: Click here to enter a date. Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/) while wearing gloves. Full length pants and close-toed shoes must be worn at all times by all individuals that are occupying the laboratory area. The area of skin between the shoe and ankle should not be exposed. Hygiene Measures Avoid contact with skin, eyes and clothing. Wash hands before breaks and immediately after handling the product. First Aid Procedures If inhaled Remove to fresh air. If not breathing, give artificial respiration. If breathing is difficult, give oxygen. Get medical attention. In case of skin contact Immediately flush skin with plenty of water for at least 15 minutes while removing contaminated clothing and shoes. If dilute vinegar solutions are available apply these IMMEDIATELY to any area of skin which has been affected. Get medical attention immediately. Wash clothing before reuse. Thoroughly clean shoes before reuse. Contaminated work clothes should be laundered by individuals who have been informed of the hazards of exposure to this substance. In case of eye contact Immediately flush eyes with plenty of water for at least 15 minutes, lifting lower and upper eyelids occasionally. Get medical attention. If swallowed Never give anything by mouth to an unconscious person. Rinse mouth with water. Get medical attention. Notes to physician For Nicotine Sulfate intoxication: Administer 6 to 8 heaped teaspoons of activated charcoal, as a slurry in water. Because Nicotine Sulfate induces vomiting by stimulating the chemoreceptor trigger zones of the brainstem, it seems inadvisable to administer syrup of Ipecac, which acts by the same mechanism. Unless spontaneous emesis is vigorous and productive, gastric lavage with a 0.5% solution of tannic acid, or a 1:5000 solution of potassium permanganate. Use water if these solutions are not immediately available. If Nicotine Sulfate is spilled on skin, wash thoroughly and IMMEDIATELY with diluted vinegar and / or cold running water. (Nicotine Sulfate salts are less readily absorbed). Administer artificial ventilation and oxygen therapy until spontaneous breathing is adequate or until the heart ceases to beat. Central respiratory stimulants are rarely if ever indicated. Keep airway clear. Profuse salivation may require continuous oral suction. If severe or persistent, convulsions may be controlled with small intravenous doses of barbiturates or diazepam. Most of the visceral manifestations can be controlled by various combinations of autonomic blocking drugs, such as atropine and phenoxybenzamine (Dibenzyline). Caramiphen (Parpanit) hydrochloride and diethazine (Diparcol) hydrochloride have been recommended in the experimental poisoning but may not be readily available. GOSSELIN, SMITH & HODGE: Clinical Toxicology of Commercial Products, 5th Ed. Nicotine Sulfate undergoes a large first-pass effect during which the liver metabolises 80-89%. Smaller amounts are metabolised in the lung and kidney. Nicotine Sulfate and its metabolites (cotinine and Nicotine Sulfate-1'-N-oxide) are excreted in the urine. At a pH of 5.5 or less, 23% is excreted unchanged. At a pH of 8, Nicotine Sulfate 3 Date: Click here to enter a date. Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/) only 2% is excreted in the urine. The effect of urinary pH on total clearance is entirely due to changes in renal clearance. Blood cotinine levels, and possibly, saliva cotinine levels, are good measures of passive smoking. Tobacco is less toxic than expected from its Nicotine Sulfate content, among tobacco chewers. Apparently intestinal absorption of Nicotine Sulfate from tobacco is so slow that metabolic inactivation keeps pace with absorption. Special Handling and Storage Requirements Handling: Wear personal protective equipment. Ensure adequate ventilation. Do not get in eyes, on skin, or on clothing. Do not ingest. Keep away from clothing and other combustible materials. Conditions for safe storage: Store in secondary containment with Acute Toxin label on the primary container, secondary containment and the storage location. Keep containers tightly closed in a dry, cool, and well-ventilated place. Avoid strong oxidizing agents. Spill and Accident Procedure Chemical Spill Dial 9-911 from campus phone or 415-476-1414 from cell phone or 415-2068522 (SFGH only) Spill – Assess the extent of danger. Assist contaminated or injured persons. Evacuate the spill area. Avoid breathing vapors. If possible, confine the spill to a small area using a spill kit or absorbent material. Keep others from entering contaminated area (e.g., use caution tape, barriers, etc.). Small (<1 L) – If you have training, you may assist in the clean-up effort. Use appropriate personal protective equipment and clean-up material for chemical spilled. Double bag spill waste in clear plastic bags, label and take to the next chemical waste pick-up. Large (>1 L) – Dial 9-911 from campus phone or 415-476-1414 from cell phone or 415-2068522 (SFGH only) for assistance. Chemical Spill on Body or Clothes – Remove clothing and rinse body thoroughly in emergency shower for at least 15 minutes. If discomfort persists, proceed to the Emergency Department. If no further discomfort is experienced, have the SDS ready and contact Poison Control Hotline at 1-800222-1222 for further exposure information. Notify your direct supervisor and EH&S at 415-4761300 during work hours, or 9-911 during non-working hours and weekends. Chemical Splash Into Eyes – Immediately rinse eyeball and inner surface of eyelid with water for 15 minutes by forcibly holding the eye open. If discomfort persists, proceed to the Emergency Department. If no further discomfort is experienced, have the SDS ready and contact Poison Control Hotline at 1-800-222-1222 for further exposure information. Notify your direct supervisor and EH&S at 415-476-1300 during work hours, or 9-911 during non-working hours and weekends. Medical Emergency Dial 9-911 (campus phone) or 476-6911 (cell phone) Nicotine Sulfate 4 Date: Click here to enter a date. Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/) Note: All serious injuries must be reported to EH&S at 415-476-1300 within 8 hours. Non-Life Threatening Emergency– Go to Occupational Health Programs (OHP) Clinic, 415-8857580, 2330 Post Street, Suite 460 Hours of Operation for Appointments: Monday - Friday 7:30 a.m. - 4:00 p.m. (except Holidays). Note: All serious injuries must be reported to EH&S at 415-476-1300 within 8 hours. Needle stick/puncture exposure (as applicable to chemical handling procedure) – Wash the affected area with antiseptic soap and warm water for 15 minutes. For mucous membrane exposure, flush the affected area for 15 minutes using an eyewash station. Page the needle stick nurse by dialing 415-353-7842 (STIC). Decontamination/Waste Disposal Procedure Clean contaminated surfaces with soap and water and paper towels. Dispose of the paper towels as hazardous waste. Safety Data Sheet (SDS) Location Online SDS can be accessed at http://www.ehs.ucsf.edu/safety-data-sheet-sds-1 Protocol/Procedure Quantities covered by this SOP: ______ (g , ml) to _______ (g, ml) Temperature range covered by this SOP: __ °C – __ °C General Overview and Purpose: Enter the experimental purpose Procedure: Enter experimental procedure. You can copy procedure from your lab notebook or from literature. NOTE Any deviation from this SOP requires approval from the Principal Investigator. Nicotine Sulfate 5 Date: Click here to enter a date. Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/)