Sequencing Report
Sequencing result
(1) No signal
Possible problem
(1-1) Insufficient DNA concentration
(1-2) Indication of primer concentration is
wrong.
Suggestion
(1-1a) Increase DNA concentration
(1-2a) Check primer concentration
(1-3) No primer annealing spot
(1-3a) Please check accuracy of the primer
(1-4) A clone problem
(1-4a) Please check whether clone is been cloned or not
(1-4b) Please check the vector is been modified or not
(2) Signal disorderly
(2-1) Low specificity of primer
(2-2) Insufficient DNA concentration
(2-3) Primer degraded (N-1, N-2..)
(2-4) Multiple templates
(2-1a) Redesign primer (Suitable Tm is between 50℃ to
55℃); or, change primer
(2-2a) Increase DNA concentration
(2-3a) Please check the storage condition of primer, or use
another new dissolved primer instead
(2-4a) No single product, please purify template again
(2-4b) Please purify single colony again
(2-5) Multiple priming sites
(2-5a) Please change another primer, which has a single
annealing spot
(2-6) Fragment shift mutation (SNP,
insertion or deletion).
(2-7) Low quality of sample (poor quality (2-7a) Please remove inhibitors completely among
DNA)
(3) Signal interrupted
earlier
sequencing reaction (salt, phenol, EDTA and etc.)
(2-8) Sample smear
(2-8a) Please prepare the sample again
(3-1) Homopoly (T),(A),(G),(C)
(3-1a) Please change the other side of primer
(3-1b) Please avoid the repeated fragment and design
primer again
(3-2) Secondary structure
(Repeat sequence, hair pin, siRNA..)
(3-2a) Please use forward/reverse primer
(3-2b) Use unwind secondary structure reagents (dGTP,
DMSO)
Order number: SD130611003
Date
Sample name
Handling method
07/15
Thermophile-1_BSR1541
We could not improve the noisy signal.
The data would not be re-tested temporarily.
Please contact with us if you have any questions.
Handler: Camilla Lai
Ext: 137
Genomics Bioscience & Technology Co., Ltd.
14F., No.108, Sec. 1, Xintai 5th Rd., Xizhi City, Taipei County 221, Taiwan (R.O.C.)
www.genomics.com.tw
+886-2-2696-1658