Table 1. Portuguese Water Dog Autopsy Protocol I. Gross Metrics Weight body weight (lb) tongue (g) brain, total (g) cerebral hemispheres (g) pons (g) cerebellar hemispheres (g) medulla oblongata (g) temporalis (g) lung right caudal lobe (g) lung right accessory lobe (g) lung right medial lobe (g) lung right cranial lobe (g) lung left caudal lobe (g) lung left medial lobe (g) lung left cranial lobe (g) heart (g) esophagus weight (g) stomach (g) pancreas (g) liver & gall bladder (g) gall bladder (g) adrenal (g) spleen (g) Method intrinsic muscles only left, right vacated of residual blood vacated of residual food both lobes left, right Length achilles tendon (mm) neck length (cm) right ear pinna (cm) left ear pinna (cm) trunk length (cm) trunk circ. (cm) trunk circ. (cm) sternum length (cm) tail length (cm) trachea length (cm) trachea height (mm) trachea width (mm) ventricle, l, width (mm) ventricle, r, width (mm) esophagus (cm) small intestine (cm) large intestine (cm) Method muscle belly to insertion occiput to Neural Spine of C7 pinna tip to skull pinna tip to skull tail base to Neural Spine of C7 level of xiphoid level of axilla manubrium to xiphoid base to tip base of cricoid to carina 3rd and 4th ring below cricoid 3rd and 4th ring below cricoid caliper thickest point caliper thickest point anterior trachea to stomach II. Histological sections Organ sampled Location of Sample skin nasal skin dorsum of back skin Right hock (ankle) eye right, all structures tongue tonsils salivary gland lymph node thyroid parathyroid brain pituitary spinal cord lung heart heart heart heart heart kidney adrenal glands urinary bladder spleen right kidney (g) liver left kidney (g) bladder & urethra (g) prostate (g) triceps (g) gluteus medius (g) quadriceps femoris group (g) liver gall bladder pancreas pancreas stomach duodenum right side right side right side papillary region right hock (ankle) right side right side cervical right right side all structures all portions C1 level mid-right caudal lobe atrioventricular valves right, left left ventricular free wall right ventricular free wall interventricular septum right atrium at SA node cross section right side right, left Inferior portion mid-cranial proximal left lateral lobe mid portion proximal right lateral lobe mid portion whole gall bladder head duodenal portion tail colonic portion cardiac mid-segment hamstrings (g) gastronemus & plantaris (g) adductor mass (g) achilles tendon (g) right side right side right side right side jejunum ileum colon colon colon bone bone bone skeletal muscle mid-segment mid-segment ascending mid-segment transverse mid-segment descending mid-segment scapular articulation, right humeral head, right marrow humeral head and neck vastus lateralis, mid portion, right side Journal: Age Title: “Age Relationships of Postmortem Observations in Portuguese Water Dogs” Authors: Chase K1, Lawler D2, McGill L3, Miller S1, Nielsen M1 and Lark KG1 1Department 21105 of Biology, University of Utah, 257 South 1400 E., Salt Lake City, UT 84112 USA Woodleaf Drive, O'Fallon, IL 62269 USA 3ARUP, Animal Reference Pathology Division, 500 Chipeta Way, Salt Lake City, UT 84108 USA Corresponding Author: Karl G. Lark, University of Utah, Department of Biology, 257 South 1400 E., Room 201 Salt Lake City, UT 84112 Ph.: 801-581-7364 Fax: 801-585-9735 E-Mail: lark@bioscience.utah.edu