Course Title: Advanced Plant and Soil Science Lesson Title: Compare Mitosis and Meiosis TEKS Addressed in Lesson: 130.21 (c) (15) (D) http://www.tea.state.tx.us/index2.aspx?id=5415#Subchapter A Lesson Objectives: The student will be able to…. 1. Compare the phases of Mitosis and Meiosis 2. Explain the significance of each process Tools and Equipment Power point entitled “Mitosis and Meiosis” YouTube video entitled “Mitosis” 6:11 http://www.youtube.com/watch?v=C6hn3sA0ip0&feature=related Power point of pictures of vegetables Mitosis notes sheet and teachers key Key Terms / Vocabulary mitosis prophase prometaphase metaphase anaphase telophase kinetochore centromere interphase G1 S phase G2 Meiosis 1 Interest Approach/Anticipatory Set At the beginning of the period, show the YouTube video entitled “Mitosis”. Afterwards show them the pictures of the vegetables from Alaska and ask the students “How did the vegetables get to such a large size?” Have them guess the weight of the potato. Teaching Plan and Strategy / Presentation of New Material Present new material using PowerPoint Presentation titled “Mitosis and Meiosis” Activity/Application/ Student Engagement /Laboratory After your introduction to mitosis, present the power point and have students fill in the notes. Evaluation / Summary Ask the students to devise a sentence to help them remember the order of the cell cycle. Then you can vote on which sentence is the best. Below is an example: I passed my algebra test, Cindy. Interphase, prophase, metaphase, anaphase, telephase, cytokinesis. References/Additional Materials / Extended Learning Opportunities/ Enrichment Postlethwait, John H. and Hopson, Janet L., et al. Modern Biology. 2nd ed. Austin, Tx: Holt, Rinehart and Winston, 2009. Campbell, Neil A., Reece, Jane B. and Mitchell, Lawrence G., et al Biology. 9th ed. Menlo Park, CA: Pearson, Benjamin Cummings, 2010 CTE Online Website. Assessed June 5, 2012 from http://www.cteonline.org/portal/default/default College & Career Readiness Standard Science VI A4 & D 5 ©Texas Education Agency, 2013 2 Mitosis and Meiosis Notes Fill and label all parts of the cell cycle: 3 Cell Cycle = Interphase and Mitosis: Draw the phases and record what occurs in each phase Phase Events that Occur Interphase Prophase Metaphase Anaphase Telophase Cytokinesis 4 Or Polar bodies/ Egg What are the purposes of Mitosis? What is the result of uncontrolled Mitosis? What is the purpose of Meiosis? Meiosis Stages Matching Events Events to Place in Order Stage Interphase o Crossing over Meiosis I Prophase I o Two daughter cells are formed o Four haploid daughter cells are formed o Chromosomes line up in Metaphase I 5 middle of cell Anaphase I Telophase I o Nuclear membrane breaks down o Nuclear membrane reforms o Spindle fibers appear Meiosis II Prophase II o Sister chromatids pulled to opposite ends of cell o Chromosomes are doubled o Nuclear membrane re- Metaphase II Anaphase II forms o Centrioles pulled to opposite ends of cell o Two new nuclei are formed Telophase II o Spindle fibers appear o Two new nuclei are formed in each cell o Nuclear membrane breaks down o Centromeres break down o Homologous chromosomes pulled to opposite ends of cell o Centrioles pulled to opposite ends of cell o Homologous chromosomes line up in middle of cell 6 Mitosis and Meiosis Notes Teacher Key Fill and label all parts of the cell cycle: G1- growth S- DNA synthesis G2- growth and organelle replication 1- prophase 2- metaphase 3- anaphase 4- telophase 5- cytokinesis 7 Cell Cycle = Interphase and Mitosis: Draw the phases and record what occurs in each phase Phase Interphase Events that Occur a cell performs all of its regular functions and gets ready to divide. Metabolic activity is very high. Cell does most of its’ growth during interphase Chromosomes are replicated Prophase Chromatin coils into visible chromosomes Centrosome: mitotic centers, poles for division migrate Nuclear envelope breaks. Microtubules (kinetochore + pole) attach to centrioles Metaphase Chromatids align on a plane at cell’s equator. Anaphase Chromatids separate simultaneously Sister chromatids become daughter chromosomes. Telophase Daughter chromosomes stop moving Chromosomes uncoil, nucleus and nucleoli reform 8 Cytokinesis Division of cell cytoplasm after mitosis Or Polar bodies/Egg What are the purposes of Mitosis? Growth, repair, asexual reproduction What is the result of uncontrolled Mitosis? cancer What is the purpose of Meiosis? Produce gametes for sexual reproduction Stage Interphase Meiosis I Meiosis Stages Matching Events Chromosomes are doubled Prophase I Crossing over Events to Place in Order o Crossing over o Two daughter cells are 9 Nuclear membrane breaks down formed o Four haploid daughter cells are formed Spindle fibers appear Centrioles pulled to opposite ends of cell Metaphase I Homologous Chromosomes line up in middle of cell Anaphase I Homologous chromosomes pulled to opposite ends of cell Telophase I Two daughter cells are formed Nuclear membrane reforms Meiosis II o Chromosomes line up in middle of cell o Nuclear membrane breaks down o Nuclear membrane reforms o Spindle fibers appear o Sister chromatids pulled to opposite ends of cell Two new nuclei are formed in each cell o Chromosomes are Spindle fibers appear Nuclear membrane breaks down o Nuclear membrane re- Prophase II doubled forms o Centrioles pulled to opposite ends of cell Metaphase II Two new nuclei are formed chromosomes line up in middle of cell formed o Spindle fibers appear Anaphase II Telophase II o Two new nuclei are Centrioles pulled to opposite ends of cell o Two new nuclei are Sister chromatids pulled to opposite ends of cell o Nuclear membrane Four haploid daughter cells are formed Nuclear membrane reforms o Centromeres break down formed in each cell breaks down o Homologous chromosomes pulled to Two new nuclei are formed opposite ends of cell o Centrioles pulled to 10 opposite ends of cell o Homologous chromosomes line up in middle of cell `Mitosis and Meiosis Reviewsheet Matching: match the term to the description A. Prophase B. Interphase C. Telophase D. Metaphase E. Anaphase _____ 1. The sister chromatids are moving apart. ___ 2. The nucleolus begins to fade from view. _____ 3. A new nuclear membrane is forming around the chromosomes. _____ 4. The cytoplasm of the cell is being divided. _____ 5. The chromosomes become invisible. _____ 6. The chromosomes are located at the equator of the cell. _____ 7. The nuclear membrane begins to fade from view. _____ 8. The division (cleavage) furrow appears. _____ 9. The chromosomes are moving towards the poles of the cell. _____ 10. Chromatids line up along the equator. _____ 11. The spindle is formed. _____ 12. Chromosomes are not visible. _____ 13. Cytokinesis is completed. _____ 14. The cell plate is completed. _____ 15. Chromosomes are replicated. _____ 16. The reverse of prophase. _____ 17. The organization phase. 11 Fill in the blank: (drag/drop or write the word) Some will be used more than once. A. Prophase D. Metaphase G. Chromatid J. Spindle fiber B. Interphase C. Telophase E. Anaphase H. Cytokinesis K. Cell Plate F. Centromere I. Mitosis E. Anaphase ________________18. What phase are daughter cells in as a result of mitosis? ________________19. During what phase of mitosis do centromeres divide and the chromosomes move toward their respective poles? ________________20. What is the phase where chromatin condenses to form chromosomes? ________________21. What is the name of the structure that connects the two chromatids? ________________22. In a chromosome pair connected by a centromere, what is each individual chromosome called? ________________23. What are the two parts of cell division? ________________ ________________24. What structure forms in prophase along which the chromosomes move? ________________25. Which phase of mitosis is the last phase that chromatids are together? ________________26. Which phase of the cell cycle is characterized by a non-dividing cell? ________________27. What structure is produced when protein fibers radiate from centrioles? ________________28. What forms across the center of a cell near the end of telophase? ________________29. The period of cell growth and development between mitotic divisions? ________________30. What is the phase where cytokinesis occurs? Review Questions: 31. During which stage of a cell’s cycle do the replicated chromosomes thicken and become visible? ______________________ 32. In animal cells, which structure is thought to produce the spindle fibers that help separate the sister chromatids during anaphase? ______________________ 12 33. Is this structure found in plant cells?__________ 5. The drawing below has been made from a photograph showing a cell undergoing mitosis. Based on the drawing, in what stage of mitosis must the cell have been in? 6. The drawings A-E show stages of mitosis in a plant cell. (a) Which of the drawings A -E shows (i) interphase ________ (DNA is replicated) (ii) prophase ________ (chromosomes – 2 sister chromatids – shorten) (iii) metaphase ________ (sister chromatids line up) (iv) anaphase ________ (sister chromatids separate) 13 (v) telophase ________ (new nucleus forms at each end) (vi) cytokinesis ________ (cell contents divided between 2 daughter cells) (b) Give two processes which occur during interphase and which are necessary for nuclear division to take place. 7. This drawing shows various stages of mitosis in a fast growing onion root tip. Identify the cells (by number) which are in the following stages of mitosis: interphase _____________ prophase _____________ metaphase _____________ anaphase _____________ telophase_____________ 14 `Mitosis and Meiosis Reviewsheet Teacher’s Key Matching: match the term to the description (drag/drop or write the letter) A. Prophase B. Interphase C. Telophase D. Metaphase E. Anaphase __E___ 1. The sister chromatids are moving apart. _ A__ 2. The nucleolus begins to fade from view. ___C__ 3. A new nuclear membrane is forming around the chromosomes. ___C__ 4. The cytoplasm of the cell is being divided. ___C__ 5. The chromosomes become invisible. ___D__ 6. The chromosomes are located at the equator of the cell. ___A__ 7. The nuclear membrane begins to fade from view. __C___ 8. The division (cleavage) furrow appears. ___E__ 9. The chromosomes are moving towards the poles of the cell. ___D__ 10. Chromatids line up along the equator. ___A__ 11. The spindle is formed. ___B__ 12. Chromosomes are not visible. __B___ 13. Cytokinesis is completed. __B___ 14. The cell plate is completed. __B___ 15. Chromosomes are replicated. __C___ 16. The reverse of prophase. __D___ 17. The organization phase. 15 Fill in the blank: (drag/drop or write the word) Some will be used more than once. A. Prophase D. Metaphase G. Chromatid J. Spindle fiber B. Interphase C. Telophase E. Anaphase H. Cytokinesis K. Cell Plate F. Centromere I. Mitosis E. Anaphase B. Interphase ________________18. What phase are daughter cells in as a result of mitosis? E. Anaphase ________________19. During what phase of mitosis do centromeres divide and the chromosomes move toward their respective poles? A. Prophase ________________20. What is the phase where chromatin condenses to form chromosomes? F. Centromere or Kinetocore ________________21. What is the name of the structure that connects the two chromatids? G.____________ Chromatid 22. In a chromosome pair connected by a centromere, what is each individual chromosome called? I. Mitosis ________________23. What are the two parts of cell division? H. Cytokinesis ________________ J. Spindle fiber ________________24. What structure forms in prophase along which the chromosomes move? D. Metaphase ________________25. Which phase of mitosis is the last phase that chromatids are together? B. Interphase ________________26. Which phase of the cell cycle is characterized by a non-dividing cell? J. Spindle fiber ________________27. What structure is produced when protein fibers radiate from centrioles? K. Cell Plate ________________28. What forms across the center of a cell near the end of telophase? B. Interphase ________________29. The period of cell growth and development between mitotic divisions? C. Telophase ________________30. What is the phase where cytokinesis occurs? Review Questions: 31. During which stage of a cell’s cycle do the replicated chromosomes thicken and become visible? ___Prophase___________________ 32. In animal cells, which structure is thought to produce the spindle fibers that help separate the sister chromatids during anaphase? _____centrioles or centrosomes__ 16 33. Is this structure found in plant cells?___yes, to centrosomes/ no to centrioles__ 5. The drawing below has been made from a photograph showing a cell undergoing mitosis. Based on the drawing, in what stage of mitosis must the cell have been in? ANAPHASE 6. The drawings A-E show stages of mitosis in a plant cell. (a) Which of the drawings A -E shows (i) interphase __c______ (DNA is replicated) (ii) prophase ____b____ (chromosomes – 2 sister chromatids – shorten) (iii) metaphase ____e____ (sister chromatids line up) 17 (iv) anaphase ______a__ (sister chromatids separate) (v) telophase ______d__ (new nucleus forms at each end) (vi) cytokinesis ___d_____ (cell contents divided between 2 daughter cells) (b) Give two processes which occur during interphase and which are necessary for nuclear division to take place. ____Growth________ of the cell and _____replication______ of the DNA 7. This drawing shows various stages of mitosis in a fast growing onion root tip. Identify the cells (by number) which are in the following stages of mitosis: interphase ___4,5,6,8,10,13,15,16,18__________ prophase ___2, 14__________ metaphase ___1, 11, 17__________ anaphase ___7, 9__________ telophase_____3, 12________ 18 Directions: 1. Label all key structures (homologues, sister chromatids, centromere, nuclear membrane, spindle fibers, nucleus). 2. Write a brief description of each phase (don’t forget crossing over). 3. Label each cell diploid or haploid. 4. Color each chromosome. a. Color one homologous pair of chromosomes dark ____red____________ and light ____blue______________. Choose the same color. b. Color the other homologous pair of chromosomes dark _____blue___________ and light ___pink_______________. Choose the same color, but different from step a. c. DON’T forget to color 19