Impacts of Green Tea Polyphenols on Drosophila melanogaster Development and Reproduction Julia C. Barbour Mentor: Mahtab Jafari Green tea has been shown to increase the life span of the male fruit fly Drosophila melanogaster while reducing its fertility. The goal of this project was to determine if green tea polyphenols (GTPs) have detrimental effects on the reproductive organs of male fruit flies by examining their morphology after exposure. I fed the flies green tea, then removed their reproductive tracts and stained them with 4,6-diamidino-2-phenylindole dihydrochloride hydrate (DAPI) for visualization using a fluorescence microscope. The flies were exposed to GTPs throughout their development from embryos to adult flies. The reproductive organs appear to be mildly affected; however, many of the flies did not make it to adulthood because the higher doses of GTPs proved to be toxic. Upon discovering delayed development and larval mortality in the higher dose groups, my focus shifted to the impaired growth. The life cycle was monitored to determine when most of the flies were dying and if the flies that made it to the adult stage showed any impairments. My findings suggest that consumption of high doses of GTPs slows fly growth and results in less robust adult flies. The reproductive impacts are still being investigated.