MCB 7300: MOLECULAR AND CELLULAR BIOLOGY LABORATORY
(Class Number 2992 – 3.0 Credit Hours)-Spring 2015
Time & Place: Mon. & Wed. 3:05 PM – 6:05 PM in the locations listed on the syllabus.
Course Coordinator: Allan Showalter
Instructors: MCB Faculty as listed on the syllabus
Text: None. Handouts and/or assigned readings will be provided by the instructors.
Requirements and Course Description:
Prerequisites: MCB 7200 or permission. The purpose of this course is to expose students to a
variety of laboratory techniques used in the broad field of molecular and cellular biology by
allowing the student to carry out these techniques in the laboratory.
Grades will be based on the following:
1. Each instructor, with the exceptions of Thuma and King, will evaluate the student based
on a 100 point scale (or 200 points in the case of the Wyatt lab and 300 points in the case of the
Kim lab, i.e., now Vis, Basu/Showalter, and Carrol labs) using one or more of the following
criteria: 1) a written lab report, 2) a laboratory examination, and 3) instructor observation of
student preparation and participation in the lab.
2. Each instructor, with the exceptions listed above, will submit student scores to the course
coordinator who will total these scores and record the final grade of the student based on a total
of 1100 possible points. (Note that the Wyatt and Kim labs will be worth 200 and 300 points
respectively, while the other labs will be worth 100 points each). A standard percentage grading
system will be used. Thus, 93.3% and above will earn an A, 90-93.3% an A-, 86.7-90% a B+,
83.3-86.7% a B, 80-83.3% a B-, 76.7-80% a C+, 73.3-76.7% a C, 70-73.3% a C-, 66.7-70% a
D+, 63.3-66.7% a D, 60-63.3% a D-, and below 60% an F.
Office Hours:
Allan Showalter- By appointment, Porter 504, phone 593-1135.
Email: showalte@ohio.edu
Academic Conduct:
The penalty for course-related academic dishonesty (i.e., plagiarism, cheating, etc.) will be failure
of the entire course.
Attendance Policy:
Attendance for laboratory is mandatory. All absences must be justified and explained to the
instructor and the course coordinator in advance. Each unjustified absence will result in the
reduction of the student's final course grade by one full letter grade.
Course web page: http://www.ohio.edu/plantbio/staff/showalte/MCB%20730/
MCB 7300: MOLECULAR AND CELLULAR BIOLOGY LABORATORY
Syllabus - Spring 2015
Instructors: MCB Faculty as listed below. Please take special note where each lab is to be held.
WEEK DATE
Laboratory (Instructor-lab location)
1
Jan. 12
Jan. 14
Introduction (Wyatt or Showalter -Porter 304)
Genomic DNA isolation, finding a gene of interest (Wyatt -Porter 304)
2
Jan. 19
Jan. 21
Martin Luther King Day (no class)
Electrophoresis, ligation & transformation (Wyatt -Porter 304)
3
Jan. 26
Jan. 28
Blue-white screening and plasmid prep (Wyatt -Porter 304)
Seahorse I (Hata-Konnecker Research Lab 126)
4
Feb. 2
Feb. 4
Restriction digestion and gel electrophoresis (Wyatt -Porter 304)
Seahorse II (Hata-Konnecker Research Lab 126)
5
Feb. 9
Feb. 11
Introduction to DNA sequencing/PCR (Vis-Porter 304)
DNA sequence analysis (Vis-Porter 304)
6
Feb. 16
Feb. 18
RNA isolation, RNA quality, qPCR setup (Basu/Showalter-Porter 304)
qPCR and qPCR analysis (Basu/Showalter-Porter 304)
7
Feb. 23
Feb. 25
Southern blot analysis I (Showalter-Porter 304)
Southern blot analysis II (Showalter-Porter 304)
8
March 2
March 4
Sprink Break (no class)
Sprink Break (no class)
9
March 9
March 11
Bioinformatics tools I (Hua-Porter 304)
Bioinformatics tools II (Hua-Porter 304)
10
March 16
March 18
Western blot analysis I (McCall-ARC 340)
Western blot analysis II (McCall-ARC 340)
11
March 23
March 25
Fluorescence-Activated Cell Sorting Lab (Goetz & Burdick-ARC 259)
Fluorescence-Activated Cell Sorting Lab (Goetz & Burdick- ARC 259)
12
March 30
April 1
Next gen sequencing (Carrol-Porter 304)
Introduction to microarrays (Carrol-Porter 304)
13
April 6
April 8
Fluorescence microscopy lab (Berryman-Porter 304)
Molecular Technologies Laboratory (J. D. Kittle- meet in Porter 304)
14
April 13
April 15
Confocal Lab I (Thuma-Wilson 014)
Confocal Lab II (Thuma-Wilson 014)
15
April 20
April 22
Diagnostic Hybrids Field Trip (Kevin King-meet in Porter 304)
Mammalian transfection lab (Lee-Konneker Research Lab 125)
Teaching assistants:
Xiao Liu: xl224810@ohio.edu
Raj Thapa: rt714114@ohio.edu